BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0885
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC9G1.07 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 28 1.2
SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate |Schizos... 26 5.0
SPAC26A3.09c |rga2||GTPase activating protein Rga2|Schizosacchar... 26 5.0
SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|c... 26 5.0
SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc... 26 6.6
SPAC17H9.05 |ebp2||rRNA processing protein Ebp2|Schizosaccharomy... 25 8.7
SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces pom... 25 8.7
SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9 |Schiz... 25 8.7
>SPAC9G1.07 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 418
Score = 28.3 bits (60), Expect = 1.2
Identities = 27/98 (27%), Positives = 37/98 (37%)
Frame = +1
Query: 445 LRDGTVALPDSYKEIMELLAEAKYFLIEELTESCLQALAKKEREAEPICRVPLITSQKEE 624
L + TVA+ +K + LIE + SC KE E P + L + +E
Sbjct: 221 LLNNTVAVKQDHKLSANTFLSSPVKLIERESNSCSTISINKEDEVVPKSPLSLDRKKVDE 280
Query: 625 QLLIMSTSKPVVKLLINRHNNKYSYTSTSDDNLLKNIE 738
S +K + I K S TS S D N E
Sbjct: 281 VEEHSSIAKLDSEEKIRETKKKNSSTSLSPDPTSDNFE 318
>SPCC4B3.10c |ipk1||inositol 1,3,4,5,6-pentakisphosphate
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 640
Score = 26.2 bits (55), Expect = 5.0
Identities = 9/31 (29%), Positives = 19/31 (61%)
Frame = -1
Query: 486 LFITVG*RYCAVTKIIQNSTEMLPTTINQNP 394
+++ + +YC V K +++ EM P + Q+P
Sbjct: 471 IYLNLNEQYCLVMKDLKDGIEMKPKWLTQSP 501
>SPAC26A3.09c |rga2||GTPase activating protein
Rga2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1275
Score = 26.2 bits (55), Expect = 5.0
Identities = 19/80 (23%), Positives = 30/80 (37%)
Frame = +1
Query: 445 LRDGTVALPDSYKEIMELLAEAKYFLIEELTESCLQALAKKEREAEPICRVPLITSQKEE 624
LRD +P E+ + T S L+ + + P VP+I Q
Sbjct: 920 LRDSMEQVPLENHREFEISDRVSELSFDSSTGSVLEIADTRRNQDAPEKHVPVIEIQSSR 979
Query: 625 QLLIMSTSKPVVKLLINRHN 684
L + V+LLI+ H+
Sbjct: 980 PSLEKTDQSTPVELLIDSHS 999
>SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 598
Score = 26.2 bits (55), Expect = 5.0
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = +1
Query: 403 IDRCGKHFGTILNYLRDGTVALPDSYKEIMELL 501
+DR G+ GT++N + D + DS EI+++L
Sbjct: 534 LDRLGEDLGTVINQMNDFSKP-DDSISEIVKVL 565
>SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1679
Score = 25.8 bits (54), Expect = 6.6
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = +1
Query: 304 YTTIGTLTKSDNMLRT 351
YTTIG LTK DN L T
Sbjct: 342 YTTIGLLTKVDNHLIT 357
>SPAC17H9.05 |ebp2||rRNA processing protein Ebp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 333
Score = 25.4 bits (53), Expect = 8.7
Identities = 20/66 (30%), Positives = 30/66 (45%)
Frame = +1
Query: 520 LIEELTESCLQALAKKEREAEPICRVPLITSQKEEQLLIMSTSKPVVKLLINRHNNKYSY 699
LI+E T L AKK+RE + + + Q+E Q T + + LL +H
Sbjct: 188 LIKEATAKKLSQQAKKQRELKKFGKQVQLAKQEERQREKKETLEK-INLLKRKHTG--GD 244
Query: 700 TSTSDD 717
+T DD
Sbjct: 245 LTTEDD 250
>SPCC364.05 |vps3||GTPase regulator Vps3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 910
Score = 25.4 bits (53), Expect = 8.7
Identities = 20/76 (26%), Positives = 34/76 (44%), Gaps = 2/76 (2%)
Frame = +1
Query: 487 IMELLAEAKYFLIEELT--ESCLQALAKKEREAEPICRVPLITSQKEEQLLIMSTSKPVV 660
I+ L+ E + L++ LT + L + K + E + P+I E LLI + K +
Sbjct: 161 IVCLVTENSFELLDILTFDTTPLFPIIKYDSENNGLMYKPMIVLHSNEFLLITGSPKDAI 220
Query: 661 KLLINRHNNKYSYTST 708
L ++ N T T
Sbjct: 221 GLFVDSQGNVTRSTLT 236
>SPAC227.11c |||sensor for misfolded ER glycoproteins Yos9
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 322
Score = 25.4 bits (53), Expect = 8.7
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = -3
Query: 274 HIVTDCLLTTFCDLPTLFQNEEITSTR*YETTSTPGLSTIFLFQM 140
H++ +T D P + Q +E++S T PGL ++ F++
Sbjct: 211 HVILSYECSTNSDTPEITQYQEVSSCAYSMTIHVPGLCSLPAFKI 255
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,957,140
Number of Sequences: 5004
Number of extensions: 58925
Number of successful extensions: 158
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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