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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0825
         (660 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_27207| Best HMM Match : eIF-5a (HMM E-Value=3.1e-15)                87   4e-31
SB_5387| Best HMM Match : DNA_pol_E_B (HMM E-Value=9.2e-35)            24   2.4  
SB_579| Best HMM Match : No HMM Matches (HMM E-Value=.)                29   3.4  
SB_22707| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.4  
SB_12083| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.4  
SB_8527| Best HMM Match : zf-C2H2 (HMM E-Value=4.3e-21)                28   7.7  

>SB_27207| Best HMM Match : eIF-5a (HMM E-Value=3.1e-15)
          Length = 710

 Score = 87.4 bits (207), Expect(2) = 4e-31
 Identities = 38/46 (82%), Positives = 43/46 (93%)
 Frame = +2

Query: 89  DSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKV 226
           +SGAS T+P QCS+LRKNG V++KGRPCKIVEMSTSKTGKHGHAKV
Sbjct: 599 ESGASDTYPAQCSSLRKNGHVVIKGRPCKIVEMSTSKTGKHGHAKV 644



 Score = 65.3 bits (152), Expect(2) = 4e-31
 Identities = 27/62 (43%), Positives = 43/62 (69%)
 Frame = +2

Query: 326 QLTDISDNGYLTLMADNGDLREDLKIPDGDLGTQLRTDFDSGKELLCTVLKSCXEECVIX 505
           ++T+I ++GYL LM DNGD R D+K+ D D+  ++R  F++ +  + TVLK+  EE V+ 
Sbjct: 643 KVTNIEEDGYLELMDDNGDTRADIKLQDNDIAKEIRAKFEASENFMVTVLKAMGEETVVG 702

Query: 506 VK 511
           VK
Sbjct: 703 VK 704


>SB_5387| Best HMM Match : DNA_pol_E_B (HMM E-Value=9.2e-35)
          Length = 458

 Score = 24.2 bits (50), Expect(2) = 2.4
 Identities = 13/53 (24%), Positives = 26/53 (49%)
 Frame = +3

Query: 111 SPCNVRPCVKMVSLC*RVVHARLLKCPHPKPESTATLKFTWLGLISSMVKSMK 269
           +PC ++ C + +S+    V   ++K P   P    TL   ++G  +  +K +K
Sbjct: 398 NPCRIQYCTQEISMTPIHVLLLIVKAPILDPSLVVTLCSRFIGHQARKLKIVK 450



 Score = 23.8 bits (49), Expect(2) = 2.4
 Identities = 8/13 (61%), Positives = 9/13 (69%)
 Frame = +3

Query: 93  PGPQPPSPCNVRP 131
           PGPQ P P N+ P
Sbjct: 362 PGPQDPGPGNILP 374


>SB_579| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 154

 Score = 29.1 bits (62), Expect = 3.4
 Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
 Frame = +2

Query: 137 KNGFVMLKGRPCKIV-EMSTSKTGKHGHAKVHLVGIDIFNG 256
           + G +M +G+PCKI   +   K G HG   +H+ G D  NG
Sbjct: 17  RRGVMMAEGKPCKITGTIEGLKAGNHGF-HIHVYG-DNTNG 55


>SB_22707| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1499

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 24/68 (35%), Positives = 33/68 (48%), Gaps = 8/68 (11%)
 Frame = +2

Query: 29  LLNSKHNNG*HRRTH-TLIPEDSGASAT-------FPMQCSALRKNGFVMLKGRPCKIVE 184
           LL SKH    HR+   +L PED  A  T        P   S++  +G+V+  G P K   
Sbjct: 73  LLGSKHG---HRKVKLSLRPEDVNAGVTTIVPGFVLPGCVSSVEDHGYVLSFGIPGKTGF 129

Query: 185 MSTSKTGK 208
           +S  KTG+
Sbjct: 130 LSKKKTGR 137


>SB_12083| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1671

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 16/43 (37%), Positives = 18/43 (41%)
 Frame = +2

Query: 251 NGKKYEDICPSTHNMDVPHVKREDYQLTDISDNGYLTLMADNG 379
           NG  Y   CP   N D    + EDY  +   D  YLT   D G
Sbjct: 400 NGHTYHMTCPGQTNFDPAKKRCEDYDCSG-RDVAYLTDQNDGG 441


>SB_8527| Best HMM Match : zf-C2H2 (HMM E-Value=4.3e-21)
          Length = 1049

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 18/70 (25%), Positives = 30/70 (42%), Gaps = 3/70 (4%)
 Frame = -1

Query: 558 NPINAELVYLSRAVF---ALTXMTHSSXQDFSTVHNNSLPLSKSVRNCVPRSPSGILRSS 388
           NP    L Y + A+F    +      S QD+  +H NS      + N +P+  S I ++ 
Sbjct: 69  NPSFGPLNYATMAIFPPNTVPAPPQDSTQDYREIHTNSFYFQHQMSNTLPKEES-IQQAD 127

Query: 387 RRSPLSAIRV 358
             +P   + V
Sbjct: 128 ESAPHDILSV 137


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,180,732
Number of Sequences: 59808
Number of extensions: 530072
Number of successful extensions: 1395
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1255
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1391
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1693527500
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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