BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0825 (660 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_27207| Best HMM Match : eIF-5a (HMM E-Value=3.1e-15) 87 4e-31 SB_5387| Best HMM Match : DNA_pol_E_B (HMM E-Value=9.2e-35) 24 2.4 SB_579| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.4 SB_22707| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.4 SB_12083| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.4 SB_8527| Best HMM Match : zf-C2H2 (HMM E-Value=4.3e-21) 28 7.7 >SB_27207| Best HMM Match : eIF-5a (HMM E-Value=3.1e-15) Length = 710 Score = 87.4 bits (207), Expect(2) = 4e-31 Identities = 38/46 (82%), Positives = 43/46 (93%) Frame = +2 Query: 89 DSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKV 226 +SGAS T+P QCS+LRKNG V++KGRPCKIVEMSTSKTGKHGHAKV Sbjct: 599 ESGASDTYPAQCSSLRKNGHVVIKGRPCKIVEMSTSKTGKHGHAKV 644 Score = 65.3 bits (152), Expect(2) = 4e-31 Identities = 27/62 (43%), Positives = 43/62 (69%) Frame = +2 Query: 326 QLTDISDNGYLTLMADNGDLREDLKIPDGDLGTQLRTDFDSGKELLCTVLKSCXEECVIX 505 ++T+I ++GYL LM DNGD R D+K+ D D+ ++R F++ + + TVLK+ EE V+ Sbjct: 643 KVTNIEEDGYLELMDDNGDTRADIKLQDNDIAKEIRAKFEASENFMVTVLKAMGEETVVG 702 Query: 506 VK 511 VK Sbjct: 703 VK 704 >SB_5387| Best HMM Match : DNA_pol_E_B (HMM E-Value=9.2e-35) Length = 458 Score = 24.2 bits (50), Expect(2) = 2.4 Identities = 13/53 (24%), Positives = 26/53 (49%) Frame = +3 Query: 111 SPCNVRPCVKMVSLC*RVVHARLLKCPHPKPESTATLKFTWLGLISSMVKSMK 269 +PC ++ C + +S+ V ++K P P TL ++G + +K +K Sbjct: 398 NPCRIQYCTQEISMTPIHVLLLIVKAPILDPSLVVTLCSRFIGHQARKLKIVK 450 Score = 23.8 bits (49), Expect(2) = 2.4 Identities = 8/13 (61%), Positives = 9/13 (69%) Frame = +3 Query: 93 PGPQPPSPCNVRP 131 PGPQ P P N+ P Sbjct: 362 PGPQDPGPGNILP 374 >SB_579| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 154 Score = 29.1 bits (62), Expect = 3.4 Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Frame = +2 Query: 137 KNGFVMLKGRPCKIV-EMSTSKTGKHGHAKVHLVGIDIFNG 256 + G +M +G+PCKI + K G HG +H+ G D NG Sbjct: 17 RRGVMMAEGKPCKITGTIEGLKAGNHGF-HIHVYG-DNTNG 55 >SB_22707| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1499 Score = 28.7 bits (61), Expect = 4.4 Identities = 24/68 (35%), Positives = 33/68 (48%), Gaps = 8/68 (11%) Frame = +2 Query: 29 LLNSKHNNG*HRRTH-TLIPEDSGASAT-------FPMQCSALRKNGFVMLKGRPCKIVE 184 LL SKH HR+ +L PED A T P S++ +G+V+ G P K Sbjct: 73 LLGSKHG---HRKVKLSLRPEDVNAGVTTIVPGFVLPGCVSSVEDHGYVLSFGIPGKTGF 129 Query: 185 MSTSKTGK 208 +S KTG+ Sbjct: 130 LSKKKTGR 137 >SB_12083| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1671 Score = 28.7 bits (61), Expect = 4.4 Identities = 16/43 (37%), Positives = 18/43 (41%) Frame = +2 Query: 251 NGKKYEDICPSTHNMDVPHVKREDYQLTDISDNGYLTLMADNG 379 NG Y CP N D + EDY + D YLT D G Sbjct: 400 NGHTYHMTCPGQTNFDPAKKRCEDYDCSG-RDVAYLTDQNDGG 441 >SB_8527| Best HMM Match : zf-C2H2 (HMM E-Value=4.3e-21) Length = 1049 Score = 27.9 bits (59), Expect = 7.7 Identities = 18/70 (25%), Positives = 30/70 (42%), Gaps = 3/70 (4%) Frame = -1 Query: 558 NPINAELVYLSRAVF---ALTXMTHSSXQDFSTVHNNSLPLSKSVRNCVPRSPSGILRSS 388 NP L Y + A+F + S QD+ +H NS + N +P+ S I ++ Sbjct: 69 NPSFGPLNYATMAIFPPNTVPAPPQDSTQDYREIHTNSFYFQHQMSNTLPKEES-IQQAD 127 Query: 387 RRSPLSAIRV 358 +P + V Sbjct: 128 ESAPHDILSV 137 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 22,180,732 Number of Sequences: 59808 Number of extensions: 530072 Number of successful extensions: 1395 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 1255 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1391 length of database: 16,821,457 effective HSP length: 79 effective length of database: 12,096,625 effective search space used: 1693527500 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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