Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0825
(660 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 185 3e-47
At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 179 1e-45
At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 175 2e-44
At5g64290.1 68418.m08076 oxoglutarate/malate translocator, putat... 28 4.8
At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-... 28 6.3
At3g49180.1 68416.m05375 transducin family protein / WD-40 repea... 28 6.3
At3g06490.1 68416.m00753 myb family transcription factor (MYB108... 28 6.3
At4g35910.1 68417.m05103 expressed protein 27 8.4
At1g56345.1 68414.m06477 pseudouridine synthase family protein l... 27 8.4
>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Arabidopsis thaliana}
Length = 158
Score = 185 bits (450), Expect = 3e-47
Identities = 81/148 (54%), Positives = 110/148 (74%), Gaps = 1/148 (0%)
Frame = +2
Query: 71 HTLIPEDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIF 250
H D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V IDIF
Sbjct: 6 HHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIF 65
Query: 251 NGKKYEDICPSTHNMDVPHVKREDYQLTDISDNGYLTLMADNGDLREDLKIPDGD-LGTQ 427
KK EDI PS+HN DVPHV R DYQL DIS++GY++L+ DNG ++DLK+P+ D L Q
Sbjct: 66 TSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQ 125
Query: 428 LRTDFDSGKELLCTVLKSCXEECVIXVK 511
+++ FD GK+L+ +V+ + EE + +K
Sbjct: 126 IKSGFDDGKDLVVSVMSAMGEEQINALK 153
>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similarity to
eukaryotic initiation factor 5A (2) (Nicotiana
plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Lycopersicon esculentum}
Length = 158
Score = 179 bits (436), Expect = 1e-45
Identities = 80/148 (54%), Positives = 108/148 (72%), Gaps = 1/148 (0%)
Frame = +2
Query: 71 HTLIPEDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIF 250
H D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V IDIF
Sbjct: 6 HHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAIDIF 65
Query: 251 NGKKYEDICPSTHNMDVPHVKREDYQLTDISDNGYLTLMADNGDLREDLKIP-DGDLGTQ 427
KK EDI PS+HN DVPHV R DYQL DIS++G+++L+ DNG ++DLK+P D L TQ
Sbjct: 66 TSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQ 125
Query: 428 LRTDFDSGKELLCTVLKSCXEECVIXVK 511
L+ F+ GK+++ +V+ + EE + +K
Sbjct: 126 LKNGFEEGKDIVVSVMSAMGEEQMCALK 153
>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similariy to
SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
(eIF-5A 1) {Lycopersicon esculentum}
Length = 159
Score = 175 bits (426), Expect = 2e-44
Identities = 78/148 (52%), Positives = 105/148 (70%), Gaps = 1/148 (0%)
Frame = +2
Query: 71 HTLIPEDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIF 250
H +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V IDIF
Sbjct: 6 HHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIF 65
Query: 251 NGKKYEDICPSTHNMDVPHVKREDYQLTDISDNGYLTLMADNGDLREDLKIP-DGDLGTQ 427
KK EDI PS+HN DVPHV R DYQL DI+++G+++L+ D+G ++DLK+P D L Q
Sbjct: 66 TAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQ 125
Query: 428 LRTDFDSGKELLCTVLKSCXEECVIXVK 511
+R FD GK+++ +V+ S EE + VK
Sbjct: 126 MRLGFDEGKDIVVSVMSSMGEEQICAVK 153
>At5g64290.1 68418.m08076 oxoglutarate/malate translocator, putative
similar to SWISS-PROT:Q41364 2-oxoglutarate/malate
translocator, chloroplast precursor. [Spinach]{Spinacia
oleracea}
Length = 563
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/71 (22%), Positives = 28/71 (39%)
Frame = +3
Query: 93 PGPQPPSPCNVRPCVKMVSLC*RVVHARLLKCPHPKPESTATLKFTWLGLISSMVKSMKI 272
P P PSP K++ L + +L+ P PE + L + S + + +
Sbjct: 80 PPPPSPSPSPSPQGAKLIPLILSISVGLILRFAVPVPEGVTPQGWQLLSIFLSTIAGLVL 139
Query: 273 SVPPHTTWTYL 305
S P W ++
Sbjct: 140 SPLPVGAWAFI 150
>At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box
RNA helicase [Chlamydomonas reinhardtii] GI:12044832;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1226
Score = 27.9 bits (59), Expect = 6.3
Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = +2
Query: 308 VKREDYQLTDISDNGYLTLMADNGDLREDLKIPDGDL-GTQLRTDFDSGKE 457
V+R+ +++ Y L ADN + + G + GT+++T+FDS +E
Sbjct: 350 VRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEEE 400
>At3g49180.1 68416.m05375 transducin family protein / WD-40 repeat
family protein contains 5 WD-40 repeats (PF00400);
GTP-binding protein beta chain homolog, Nicotiana
tabacum, PIR:T16970
Length = 438
Score = 27.9 bits (59), Expect = 6.3
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -3
Query: 523 SCVCFDGDDALLTAGFQHGA 464
+C+ F GDD+LL +G Q G+
Sbjct: 124 TCLVFSGDDSLLVSGSQDGS 143
>At3g06490.1 68416.m00753 myb family transcription factor (MYB108)
identical to transcription factor MYB108 GI:15375290
from [Arabidopsis thaliana]
Length = 323
Score = 27.9 bits (59), Expect = 6.3
Identities = 17/56 (30%), Positives = 27/56 (48%), Gaps = 2/56 (3%)
Frame = +3
Query: 168 HARLLKCPHPKPESTATLKFTWLGLISSMVKSMKIS--VPPHTTWTYLT*SAKTTN 329
HA+ LKC + T+K+ W+ + ++S S TT T T SA T++
Sbjct: 122 HAKQLKCDVNSQQFKDTMKYLWMPRLVERIQSASASSAAAATTTTTTTTGSAGTSS 177
>At4g35910.1 68417.m05103 expressed protein
Length = 458
Score = 27.5 bits (58), Expect = 8.4
Identities = 13/30 (43%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
Frame = -2
Query: 110 RWLRPRSLPVSKCVFFDVTHCCVLN-LTTV 24
RW P LP+ CV ++T C+L+ L TV
Sbjct: 260 RWKVPIVLPLRDCVRLEITRLCLLDGLKTV 289
>At1g56345.1 68414.m06477 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 322
Score = 27.5 bits (58), Expect = 8.4
Identities = 21/69 (30%), Positives = 36/69 (52%), Gaps = 1/69 (1%)
Frame = -3
Query: 475 QHGAQQFLAAVEVSTQLCAEVTIRDFEVLTQ-VTVVSHQGQVAIIRDISQLVVFALHVRY 299
+HGA + AA++V L +RD E + V+V S + + + D++ ++V A R
Sbjct: 176 KHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIV-AEGER- 233
Query: 298 VHVVCGGTD 272
+ CGG D
Sbjct: 234 -ELSCGGDD 241
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,981,946
Number of Sequences: 28952
Number of extensions: 342684
Number of successful extensions: 934
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 899
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 931
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1383534864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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