Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0822
(664 letters)
Database: tribolium
336 sequences; 122,585 total letters
Searching.......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF506022-1|AAM46898.1| 685|Tribolium castaneum polyubiquitin pr... 286 1e-79
AF506020-1|AAM46896.1| 112|Tribolium castaneum polyubiquitin pr... 222 2e-60
AF506023-1|AAM46899.1| 76|Tribolium castaneum polyubiquitin pr... 146 1e-37
AF506021-1|AAM46897.1| 64|Tribolium castaneum polyubiquitin pr... 128 5e-32
AM712901-1|CAN84640.1| 205|Tribolium castaneum hypothetical pro... 23 1.7
>AF506022-1|AAM46898.1| 685|Tribolium castaneum polyubiquitin
protein.
Length = 685
Score = 286 bits (701), Expect = 1e-79
Identities = 142/153 (92%), Positives = 142/153 (92%)
Frame = +2
Query: 56 MLIFVKTLTGKTITLEVEASDTIXNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 235
M IFVKTLTGKTITLEVE SDTI NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 236 XQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDTIENVKAKIXDKEGIPPDQQRLI 415
QKESTLHLVLRLRGGMQIFVKTLTGKTITLEVE SDTIENVKAKI DKEGIPPDQQRLI
Sbjct: 61 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI 120
Query: 416 FAGKQLEDGRTLSDYNIQKEXTLHXXXRLXGGM 514
FAGKQLEDGRTLSDYNIQKE TLH RL GGM
Sbjct: 121 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGM 153
Score = 286 bits (701), Expect = 1e-79
Identities = 142/153 (92%), Positives = 142/153 (92%)
Frame = +2
Query: 56 MLIFVKTLTGKTITLEVEASDTIXNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 235
M IFVKTLTGKTITLEVE SDTI NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 77 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 136
Query: 236 XQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDTIENVKAKIXDKEGIPPDQQRLI 415
QKESTLHLVLRLRGGMQIFVKTLTGKTITLEVE SDTIENVKAKI DKEGIPPDQQRLI
Sbjct: 137 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI 196
Query: 416 FAGKQLEDGRTLSDYNIQKEXTLHXXXRLXGGM 514
FAGKQLEDGRTLSDYNIQKE TLH RL GGM
Sbjct: 197 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGM 229
Score = 286 bits (701), Expect = 1e-79
Identities = 142/153 (92%), Positives = 142/153 (92%)
Frame = +2
Query: 56 MLIFVKTLTGKTITLEVEASDTIXNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 235
M IFVKTLTGKTITLEVE SDTI NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 153 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 212
Query: 236 XQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDTIENVKAKIXDKEGIPPDQQRLI 415
QKESTLHLVLRLRGGMQIFVKTLTGKTITLEVE SDTIENVKAKI DKEGIPPDQQRLI
Sbjct: 213 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI 272
Query: 416 FAGKQLEDGRTLSDYNIQKEXTLHXXXRLXGGM 514
FAGKQLEDGRTLSDYNIQKE TLH RL GGM
Sbjct: 273 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGM 305
Score = 286 bits (701), Expect = 1e-79
Identities = 142/153 (92%), Positives = 142/153 (92%)
Frame = +2
Query: 56 MLIFVKTLTGKTITLEVEASDTIXNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 235
M IFVKTLTGKTITLEVE SDTI NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 229 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 288
Query: 236 XQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDTIENVKAKIXDKEGIPPDQQRLI 415
QKESTLHLVLRLRGGMQIFVKTLTGKTITLEVE SDTIENVKAKI DKEGIPPDQQRLI
Sbjct: 289 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI 348
Query: 416 FAGKQLEDGRTLSDYNIQKEXTLHXXXRLXGGM 514
FAGKQLEDGRTLSDYNIQKE TLH RL GGM
Sbjct: 349 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGM 381
Score = 286 bits (701), Expect = 1e-79
Identities = 142/153 (92%), Positives = 142/153 (92%)
Frame = +2
Query: 56 MLIFVKTLTGKTITLEVEASDTIXNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 235
M IFVKTLTGKTITLEVE SDTI NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 457 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 516
Query: 236 XQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDTIENVKAKIXDKEGIPPDQQRLI 415
QKESTLHLVLRLRGGMQIFVKTLTGKTITLEVE SDTIENVKAKI DKEGIPPDQQRLI
Sbjct: 517 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI 576
Query: 416 FAGKQLEDGRTLSDYNIQKEXTLHXXXRLXGGM 514
FAGKQLEDGRTLSDYNIQKE TLH RL GGM
Sbjct: 577 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGM 609
Score = 284 bits (696), Expect = 5e-79
Identities = 141/152 (92%), Positives = 141/152 (92%)
Frame = +2
Query: 56 MLIFVKTLTGKTITLEVEASDTIXNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 235
M IFVKTLTGKTITLEVE SDTI NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 533 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 592
Query: 236 XQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDTIENVKAKIXDKEGIPPDQQRLI 415
QKESTLHLVLRLRGGMQIFVKTLTGKTITLEVE SDTIENVKAKI DKEGIPPDQQRLI
Sbjct: 593 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI 652
Query: 416 FAGKQLEDGRTLSDYNIQKEXTLHXXXRLXGG 511
FAGKQLEDGRTLSDYNIQKE TLH RL GG
Sbjct: 653 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 684
Score = 283 bits (695), Expect = 7e-79
Identities = 141/153 (92%), Positives = 141/153 (92%)
Frame = +2
Query: 56 MLIFVKTLTGKTITLEVEASDTIXNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 235
M IFVKTLTGKTITLEVE SDTI NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 305 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 364
Query: 236 XQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDTIENVKAKIXDKEGIPPDQQRLI 415
QKESTLHLVLRLRGGMQIFVKTLTGKTITLEVE SDTIENVKAKI DKEGIPPDQQR I
Sbjct: 365 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRSI 424
Query: 416 FAGKQLEDGRTLSDYNIQKEXTLHXXXRLXGGM 514
FAGKQLEDGRTLSDYNIQKE TLH RL GGM
Sbjct: 425 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGM 457
Score = 283 bits (695), Expect = 7e-79
Identities = 141/153 (92%), Positives = 141/153 (92%)
Frame = +2
Query: 56 MLIFVKTLTGKTITLEVEASDTIXNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 235
M IFVKTLTGKTITLEVE SDTI NVKAKIQDKEGIPPDQQR IFAGKQLEDGRTLSDYN
Sbjct: 381 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRSIFAGKQLEDGRTLSDYN 440
Query: 236 XQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEASDTIENVKAKIXDKEGIPPDQQRLI 415
QKESTLHLVLRLRGGMQIFVKTLTGKTITLEVE SDTIENVKAKI DKEGIPPDQQRLI
Sbjct: 441 IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI 500
Query: 416 FAGKQLEDGRTLSDYNIQKEXTLHXXXRLXGGM 514
FAGKQLEDGRTLSDYNIQKE TLH RL GGM
Sbjct: 501 FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGM 533
>AF506020-1|AAM46896.1| 112|Tribolium castaneum polyubiquitin
protein.
Length = 112
Score = 222 bits (542), Expect = 2e-60
Identities = 108/112 (96%), Positives = 108/112 (96%)
Frame = +2
Query: 140 KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNXQKESTLHLVLRLRGGMQIFVKTLTGKT 319
KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN QKESTLHLVLRLRGGMQIFVKTLTGKT
Sbjct: 1 KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKT 60
Query: 320 ITLEVEASDTIENVKAKIXDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKE 475
ITLEVE SDTIENVKAKI DKEGIPPDQQR IFAGKQLEDGRTLSDYNIQKE
Sbjct: 61 ITLEVEPSDTIENVKAKIQDKEGIPPDQQRSIFAGKQLEDGRTLSDYNIQKE 112
Score = 120 bits (290), Expect = 8e-30
Identities = 59/64 (92%), Positives = 59/64 (92%)
Frame = +2
Query: 56 MLIFVKTLTGKTITLEVEASDTIXNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 235
M IFVKTLTGKTITLEVE SDTI NVKAKIQDKEGIPPDQQR IFAGKQLEDGRTLSDYN
Sbjct: 49 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRSIFAGKQLEDGRTLSDYN 108
Query: 236 XQKE 247
QKE
Sbjct: 109 IQKE 112
Score = 91.9 bits (218), Expect = 4e-21
Identities = 43/49 (87%), Positives = 43/49 (87%)
Frame = +2
Query: 368 KIXDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKEXTLHXXXRLXGGM 514
KI DKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKE TLH RL GGM
Sbjct: 1 KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGM 49
>AF506023-1|AAM46899.1| 76|Tribolium castaneum polyubiquitin
protein.
Length = 76
Score = 146 bits (355), Expect = 1e-37
Identities = 72/76 (94%), Positives = 72/76 (94%)
Frame = +2
Query: 56 MLIFVKTLTGKTITLEVEASDTIXNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 235
M IFVKTLTGKTITLEVE SDTI NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 236 XQKESTLHLVLRLRGG 283
QKESTLHLVLRLRGG
Sbjct: 61 IQKESTLHLVLRLRGG 76
Score = 141 bits (341), Expect = 5e-36
Identities = 69/76 (90%), Positives = 69/76 (90%)
Frame = +2
Query: 284 MQIFVKTLTGKTITLEVEASDTIENVKAKIXDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 463
MQIFVKTLTGKTITLEVE SDTIENVKAKI DKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 464 IQKEXTLHXXXRLXGG 511
IQKE TLH RL GG
Sbjct: 61 IQKESTLHLVLRLRGG 76
>AF506021-1|AAM46897.1| 64|Tribolium castaneum polyubiquitin
protein.
Length = 64
Score = 128 bits (308), Expect = 5e-32
Identities = 62/64 (96%), Positives = 62/64 (96%)
Frame = +2
Query: 284 MQIFVKTLTGKTITLEVEASDTIENVKAKIXDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 463
MQIFVKTLTGKTITLEVE SDTIENVKAKI DKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 464 IQKE 475
IQKE
Sbjct: 61 IQKE 64
Score = 123 bits (296), Expect = 2e-30
Identities = 60/64 (93%), Positives = 60/64 (93%)
Frame = +2
Query: 56 MLIFVKTLTGKTITLEVEASDTIXNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 235
M IFVKTLTGKTITLEVE SDTI NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
Sbjct: 1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN 60
Query: 236 XQKE 247
QKE
Sbjct: 61 IQKE 64
>AM712901-1|CAN84640.1| 205|Tribolium castaneum hypothetical
protein protein.
Length = 205
Score = 23.4 bits (48), Expect = 1.7
Identities = 9/31 (29%), Positives = 16/31 (51%)
Frame = +2
Query: 14 DNFNNFCEFQKETTMLIFVKTLTGKTITLEV 106
+ + FC K + I+V T+ ++TL V
Sbjct: 32 EKYRKFCRILKYFIIAIYVLTILTSSVTLHV 62
Database: tribolium
Posted date: Oct 23, 2007 1:18 PM
Number of letters in database: 122,585
Number of sequences in database: 336
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 121,482
Number of Sequences: 336
Number of extensions: 2275
Number of successful extensions: 19
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17
length of database: 122,585
effective HSP length: 55
effective length of database: 104,105
effective search space used: 17177325
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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