BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0789 (449 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g07220.1 68414.m00768 expressed protein 40 6e-04 At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to ... 29 1.9 At5g42370.1 68418.m05159 expressed protein 29 1.9 At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identic... 29 1.9 At2g19150.1 68415.m02235 pectinesterase family protein contains ... 29 1.9 At1g60230.1 68414.m06783 radical SAM domain-containing protein ... 29 1.9 At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipox... 28 2.5 At5g22390.1 68418.m02612 expressed protein 27 4.4 At1g10930.1 68414.m01255 DNA helicase (RECQl4A) nearly identical... 27 4.4 At2g05210.1 68415.m00549 expressed protein 27 5.9 At2g03890.2 68415.m00352 phosphatidylinositol 3- and 4-kinase fa... 27 5.9 At2g03890.1 68415.m00351 phosphatidylinositol 3- and 4-kinase fa... 27 5.9 At5g56500.1 68418.m07051 chaperonin, putative similar to SWISS-P... 27 7.7 At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 27 7.7 At1g76965.1 68414.m08961 glycine-rich protein 27 7.7 At1g50410.1 68414.m05650 SNF2 domain-containing protein / helica... 27 7.7 >At1g07220.1 68414.m00768 expressed protein Length = 507 Score = 40.3 bits (90), Expect = 6e-04 Identities = 27/88 (30%), Positives = 42/88 (47%), Gaps = 1/88 (1%) Frame = -3 Query: 438 RIRFPSKPDTPRSSEPILIPKLRIQFADFP-YLHYSID*RLFTLETCCGYGYEPARHLHV 262 R+R P K +PRS +L+ L + F F L Y +D + +T G+ EP H+ Sbjct: 6 RLRLPHK-SSPRSPSYLLLCVLALSFFSFTALLFYKVDDFIAQTKTLAGHNLEPTPW-HI 63 Query: 261 HPSPEFSRSAESIRTPPQMRCSSRSEPY 178 P FS + + + ++CS S PY Sbjct: 64 FPRKSFSAATKHSQAYRILQCSYFSCPY 91 >At5g45900.1 68418.m05645 autophagy 7 (APG7) nearly identical to autophagy 7 [Arabidopsis thaliana] GI:19912147; contains Pfam profile PF00899: ThiF family Length = 697 Score = 28.7 bits (61), Expect = 1.9 Identities = 13/45 (28%), Positives = 25/45 (55%) Frame = -1 Query: 236 PQRVSGHRRKCGALRVPNHISLL*DSMELERSGRKENSSRTSRRR 102 P ++G CG +V NH++LL +S+ L+ ++S +R + Sbjct: 42 PISITGFYGPCGHPQVSNHLTLLSESLPLDEQSLIASTSHGNRNK 86 >At5g42370.1 68418.m05159 expressed protein Length = 447 Score = 28.7 bits (61), Expect = 1.9 Identities = 14/38 (36%), Positives = 20/38 (52%), Gaps = 1/38 (2%) Frame = -2 Query: 175 PFYRIPWNSNAQAEKKTLPGPLGGVFRPL-WVTPSNTR 65 P Y + + Q+ +K +P PL + R L W TPS R Sbjct: 285 PLYDVTSSGLVQSVEKVVPRPLRSIVRLLFWYTPSTMR 322 >At3g13320.1 68416.m01677 calcium exchanger (CAX2) almost identical to low affinity calcium antiporter CAX2 (GI:1488267) [Arabidopsis thaliana]; Ca2+:Cation Antiporter (CaCA) Family member PMID:11500563 Length = 441 Score = 28.7 bits (61), Expect = 1.9 Identities = 15/46 (32%), Positives = 27/46 (58%), Gaps = 2/46 (4%) Frame = +2 Query: 38 SIIVPSSLKTSVRRGNPKWPEDAAERSGKSFLFC--LSVRVPWNPI 169 S++ SL TS + PK P+++ S K +FC L++ +P+ P+ Sbjct: 41 SLMEQGSLSTSFPQHTPKAPKNSVLNSIKIVIFCNKLNLLLPFGPL 86 >At2g19150.1 68415.m02235 pectinesterase family protein contains Pfam profile: PF01095 pectinesterase Length = 339 Score = 28.7 bits (61), Expect = 1.9 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = +2 Query: 206 ICGGVRILSADLENSGEGCTWRCRAGSYPYPQQV 307 I GG+ + ++ EGCT + R G YP P +V Sbjct: 185 ISGGIDFIFGGAQSIFEGCTLKLRVGIYP-PNEV 217 >At1g60230.1 68414.m06783 radical SAM domain-containing protein contains Pfam profile PF04055: radical SAM domain protein Length = 458 Score = 28.7 bits (61), Expect = 1.9 Identities = 16/39 (41%), Positives = 22/39 (56%) Frame = -3 Query: 123 FPDLSAASSGHFGLPRRTLVFKDEGTIIETVPLPGXRNR 7 F D+ +AS G R+ L D+G +IETV +P R R Sbjct: 161 FKDIRSASDG----TRKILFTLDDGLVIETVVIPCDRGR 195 >At1g72520.1 68414.m08386 lipoxygenase, putative similar to lipoxygenase gi:1495804 [Solanum tuberosum], gi:1654140 [Lycopersicon esculentum], GB:CAB56692 [Arabidopsis thaliana] Length = 926 Score = 28.3 bits (60), Expect = 2.5 Identities = 14/33 (42%), Positives = 19/33 (57%) Frame = -3 Query: 231 ESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQK 133 +S + P R ++PYLPS G RTLR+K Sbjct: 215 QSQKDHPSKRILFTNQPYLPSETPSGLRTLREK 247 >At5g22390.1 68418.m02612 expressed protein Length = 202 Score = 27.5 bits (58), Expect = 4.4 Identities = 12/22 (54%), Positives = 14/22 (63%) Frame = +2 Query: 110 ERSGKSFLFCLSVRVPWNPIEG 175 + S KSFL LS PWNP +G Sbjct: 17 DNSPKSFLDTLSSSSPWNPSKG 38 >At1g10930.1 68414.m01255 DNA helicase (RECQl4A) nearly identical to DNA Helicase [Arabidopsis thaliana] GI:11121449 Length = 1188 Score = 27.5 bits (58), Expect = 4.4 Identities = 14/30 (46%), Positives = 18/30 (60%) Frame = -3 Query: 273 HLHVHPSPEFSRSAESIRTPPQMRCSSRSE 184 H H +P+ S S S+RTPP R +SR E Sbjct: 229 HYHSTSTPQPSVSNFSLRTPPVDRSASRLE 258 >At2g05210.1 68415.m00549 expressed protein Length = 364 Score = 27.1 bits (57), Expect = 5.9 Identities = 18/52 (34%), Positives = 24/52 (46%) Frame = -3 Query: 231 ESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQKRKLFPDLSAASSGHFGLPR 76 +S T C+ +E Y +GF T TLR+KR L S +F PR Sbjct: 282 DSTATLEAFLCNKDAE-YFWGLGFQDTETLRKKRNWL--LGIRESSNFVAPR 330 >At2g03890.2 68415.m00352 phosphatidylinositol 3- and 4-kinase family protein low similarity to phosphatidylinositol 4-kinase type-II beta [Homo sapiens] GI:20159767; contains Pfam profile PF00454: Phosphatidylinositol 3- and 4-kinase Length = 530 Score = 27.1 bits (57), Expect = 5.9 Identities = 16/40 (40%), Positives = 23/40 (57%) Frame = -1 Query: 158 MELERSGRKENSSRTSRRRLQATLGYPVEHSFLKTRERLL 39 MEL+RS ++ T +RRLQ L +P E S L + +L Sbjct: 60 MELDRS----DNVHTVKRRLQIALNFPTEESSLTYGDMVL 95 >At2g03890.1 68415.m00351 phosphatidylinositol 3- and 4-kinase family protein low similarity to phosphatidylinositol 4-kinase type-II beta [Homo sapiens] GI:20159767; contains Pfam profile PF00454: Phosphatidylinositol 3- and 4-kinase Length = 650 Score = 27.1 bits (57), Expect = 5.9 Identities = 16/40 (40%), Positives = 23/40 (57%) Frame = -1 Query: 158 MELERSGRKENSSRTSRRRLQATLGYPVEHSFLKTRERLL 39 MEL+RS ++ T +RRLQ L +P E S L + +L Sbjct: 60 MELDRS----DNVHTVKRRLQIALNFPTEESSLTYGDMVL 95 >At5g56500.1 68418.m07051 chaperonin, putative similar to SWISS-PROT:P08927- RuBisCO subunit binding-protein beta subunit, chloroplast precursor (60 kDa chaperonin beta subunit, CPN-60 beta) [Pisum sativum]; contains Pfam:PF00118 domain, TCP-1/cpn60 chaperonin family Length = 597 Score = 26.6 bits (56), Expect = 7.7 Identities = 17/50 (34%), Positives = 24/50 (48%) Frame = -3 Query: 237 SAESIRTPPQMRCSSRSEPYLPSIGFHGTRTLRQKRKLFPDLSAASSGHF 88 S S PP R SS + S F T+++ Q++ FP + AA HF Sbjct: 10 SMGSSLAPPSNRLSSFVS--ISSSSFGRTQSIAQRKARFPKIYAAKQLHF 57 >At3g02270.1 68416.m00208 eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein similar to SP|Q13144 Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP exchange factor) {Homo sapiens}; contains Pfam profile PF02020: eIF4-gamma/eIF5/eIF2-epsilon Length = 676 Score = 26.6 bits (56), Expect = 7.7 Identities = 10/22 (45%), Positives = 16/22 (72%) Frame = -3 Query: 192 RSEPYLPSIGFHGTRTLRQKRK 127 R+ PY+P + F G RTL+ +R+ Sbjct: 288 RTFPYVPDMKFSGNRTLKLERQ 309 >At1g76965.1 68414.m08961 glycine-rich protein Length = 158 Score = 26.6 bits (56), Expect = 7.7 Identities = 11/31 (35%), Positives = 16/31 (51%) Frame = -3 Query: 447 GTGRIRFPSKPDTPRSSEPILIPKLRIQFAD 355 G + FP KP+ P P +P+L + F D Sbjct: 94 GAAIVVFPKKPEEPVKVVPTPMPQLNLFFGD 124 >At1g50410.1 68414.m05650 SNF2 domain-containing protein / helicase domain-containing protein / RING finger domain-containing protein similar to transcription factor RUSH-1alpha [Oryctolagus cuniculus] GI:1655930; contains Pfam profiles PF00271: Helicase conserved C-terminal domain, PF00176: SNF2 family N-terminal domain, PF00097: Zinc finger, C3HC4 type (RING finger) Length = 981 Score = 26.6 bits (56), Expect = 7.7 Identities = 14/43 (32%), Positives = 19/43 (44%) Frame = -1 Query: 338 ILSTRGSSPWRPAADMGTNRRDISTYIPHLNFQGPQRVSGHRR 210 I TR P G++RRD+ST PH +GH + Sbjct: 15 IQETRTRPQHPPRIAEGSHRRDLSTLRPHFLSGSSSGANGHTK 57 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,761,069 Number of Sequences: 28952 Number of extensions: 232714 Number of successful extensions: 687 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 671 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 687 length of database: 12,070,560 effective HSP length: 75 effective length of database: 9,899,160 effective search space used: 732537840 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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