Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0785
(650 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_27207| Best HMM Match : eIF-5a (HMM E-Value=3.1e-15) 87 2e-32
SB_5387| Best HMM Match : DNA_pol_E_B (HMM E-Value=9.2e-35) 25 1.8
SB_8527| Best HMM Match : zf-C2H2 (HMM E-Value=4.3e-21) 30 1.9
SB_579| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.3
SB_12083| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.3
SB_4237| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.3
SB_7383| Best HMM Match : SapA (HMM E-Value=1.2e-13) 28 5.7
SB_20312| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.6
SB_6613| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.6
>SB_27207| Best HMM Match : eIF-5a (HMM E-Value=3.1e-15)
Length = 710
Score = 86.6 bits (205), Expect(2) = 2e-32
Identities = 38/45 (84%), Positives = 42/45 (93%)
Frame = +2
Query: 89 SGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKV 223
SGAS T+P QCS+LRKNG V++KGRPCKIVEMSTSKTGKHGHAKV
Sbjct: 600 SGASDTYPAQCSSLRKNGHVVIKGRPCKIVEMSTSKTGKHGHAKV 644
Score = 70.5 bits (165), Expect(2) = 2e-32
Identities = 29/62 (46%), Positives = 44/62 (70%)
Frame = +2
Query: 323 QLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEECVIA 502
++T+I +DGYL LM DNGD R D+K+ D D+ ++R F++ + + TVLK+ GEE V+
Sbjct: 643 KVTNIEEDGYLELMDDNGDTRADIKLQDNDIAKEIRAKFEASENFMVTVLKAMGEETVVG 702
Query: 503 VK 508
VK
Sbjct: 703 VK 704
>SB_5387| Best HMM Match : DNA_pol_E_B (HMM E-Value=9.2e-35)
Length = 458
Score = 24.6 bits (51), Expect(2) = 1.8
Identities = 13/53 (24%), Positives = 26/53 (49%)
Frame = +3
Query: 108 SPCNVRPCVKTVSLC*RVVHARLLKCPHPKPESTATLKFTWLGLISSMVKSMK 266
+PC ++ C + +S+ V ++K P P TL ++G + +K +K
Sbjct: 398 NPCRIQYCTQEISMTPIHVLLLIVKAPILDPSLVVTLCSRFIGHQARKLKIVK 450
Score = 23.8 bits (49), Expect(2) = 1.8
Identities = 8/13 (61%), Positives = 9/13 (69%)
Frame = +3
Query: 90 PGPQPPSPCNVRP 128
PGPQ P P N+ P
Sbjct: 362 PGPQDPGPGNILP 374
>SB_8527| Best HMM Match : zf-C2H2 (HMM E-Value=4.3e-21)
Length = 1049
Score = 29.9 bits (64), Expect = 1.9
Identities = 19/70 (27%), Positives = 31/70 (44%), Gaps = 3/70 (4%)
Frame = -3
Query: 555 NPINAELVYLSRAVF---ALTAMTHSSPQDFSTVHNNSLPLSKSVRNCVPRSPSGILRSS 385
NP L Y + A+F + A S QD+ +H NS + N +P+ S I ++
Sbjct: 69 NPSFGPLNYATMAIFPPNTVPAPPQDSTQDYREIHTNSFYFQHQMSNTLPKEES-IQQAD 127
Query: 384 RRSPLSAIRV 355
+P + V
Sbjct: 128 ESAPHDILSV 137
>SB_579| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 154
Score = 29.1 bits (62), Expect = 3.3
Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
Frame = +2
Query: 134 KNGFVMLKGRPCKIV-EMSTSKTGKHGHAKVHLVGIDIFNG 253
+ G +M +G+PCKI + K G HG +H+ G D NG
Sbjct: 17 RRGVMMAEGKPCKITGTIEGLKAGNHGF-HIHVYG-DNTNG 55
>SB_12083| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1671
Score = 28.7 bits (61), Expect = 4.3
Identities = 16/43 (37%), Positives = 18/43 (41%)
Frame = +2
Query: 248 NGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNG 376
NG Y CP N D + EDY + D YLT D G
Sbjct: 400 NGHTYHMTCPGQTNFDPAKKRCEDYDCSG-RDVAYLTDQNDGG 441
>SB_4237| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1438
Score = 28.7 bits (61), Expect = 4.3
Identities = 21/84 (25%), Positives = 37/84 (44%)
Frame = +2
Query: 275 PSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRTDFDSGKE 454
P + + V + +++ D + ADN + RE+ + +L ++ T G
Sbjct: 284 PESDGIRVDETENGEHEAVDELPEDKPDTEADNYEQREETPTKEDELKSECTTSDSEGTP 343
Query: 455 LLCTVLKSCGEECVIAVKANTALD 526
T KS GEE V A ++ +LD
Sbjct: 344 SAATYGKSDGEENV-AQESEESLD 366
>SB_7383| Best HMM Match : SapA (HMM E-Value=1.2e-13)
Length = 492
Score = 28.3 bits (60), Expect = 5.7
Identities = 17/44 (38%), Positives = 22/44 (50%)
Frame = -3
Query: 483 PQDFSTVHNNSLPLSKSVRNCVPRSPSGILRSSRRSPLSAIRVR 352
P+ S V + +LP+ V C+P SPS I RS P VR
Sbjct: 253 PRQISNVRSLTLPVRYQVIGCLP-SPSDIKRSVPYPPRQISNVR 295
>SB_20312| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 98
Score = 27.9 bits (59), Expect = 7.6
Identities = 15/51 (29%), Positives = 26/51 (50%), Gaps = 3/51 (5%)
Frame = -3
Query: 564 KFVNPINAELVY---LSRAVFALTAMTHSSPQDFSTVHNNSLPLSKSVRNC 421
KF++P +A + +R+ + T M+H SP + +T + KS R C
Sbjct: 23 KFIDPTSARISNQKAANRSQQSATQMSHKSPTNCTTCRMSDFSAGKSRREC 73
>SB_6613| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 523
Score = 27.9 bits (59), Expect = 7.6
Identities = 21/72 (29%), Positives = 32/72 (44%), Gaps = 4/72 (5%)
Frame = +2
Query: 290 MDVPHVKREDYQLTDISDDGYLT----LMADNGDLREDLKIPDGDLGTQLRTDFDSGKEL 457
+D+P+ K + + L ++DD L+ + D L E L PD + R D G E
Sbjct: 37 LDIPYEKYDKFDLESLTDDECLSEFRFIKNDLYRLNEALNFPD-QITCPNRLTVD-GMEA 94
Query: 458 LCTVLKSCGEEC 493
LC L+ C
Sbjct: 95 LCMTLRRFAYPC 106
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,995,594
Number of Sequences: 59808
Number of extensions: 478717
Number of successful extensions: 1292
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1287
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1657237625
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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