Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0776
(730 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_55143| Best HMM Match : Glyco_hydro_18 (HMM E-Value=0) 91 8e-19
SB_25657| Best HMM Match : Glyco_hydro_18 (HMM E-Value=0) 77 2e-14
SB_38742| Best HMM Match : No HMM Matches (HMM E-Value=.) 76 3e-14
SB_24154| Best HMM Match : Glyco_hydro_18 (HMM E-Value=2.2e-32) 76 3e-14
SB_28916| Best HMM Match : No HMM Matches (HMM E-Value=.) 76 3e-14
SB_48525| Best HMM Match : Glyco_hydro_18 (HMM E-Value=0) 65 6e-11
SB_17224| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 0.41
SB_47830| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.7
SB_12527| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.9
SB_27365| Best HMM Match : PID (HMM E-Value=1.90001e-40) 28 6.7
SB_46249| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 8.9
>SB_55143| Best HMM Match : Glyco_hydro_18 (HMM E-Value=0)
Length = 270
Score = 91.1 bits (216), Expect = 8e-19
Identities = 53/131 (40%), Positives = 74/131 (56%), Gaps = 2/131 (1%)
Frame = +1
Query: 49 TLAVLASCAALVQCADSRARIVCYFSNWAVYRPGVGRYGIEDIPVDLCTHLIYSFIGVTE 228
TL VLA A CA VCY +NW+ YR G ++ EDIP DLCTHL+YSF + +
Sbjct: 5 TLLVLAVAA----CATGNYVRVCYHTNWSQYRQGRAKFWPEDIPADLCTHLMYSFAKINQ 60
Query: 229 KSSEVLIIDPELDVDKSGFRNFTSLRSKHPDVKFMVAVGGW--AEGGSKYSHMVAQKSTR 402
K+ + E + DK + F +L+ K+P++K ++AVGGW S +S MV +TR
Sbjct: 61 KNELAMY---EWNDDKL-YPRFNALKQKNPELKTLLAVGGWNHENANSPFSRMVKTAATR 116
Query: 403 MSFIRRGCRLL 435
FI R+L
Sbjct: 117 KVFIDSVIRIL 127
Score = 50.0 bits (114), Expect = 2e-06
Identities = 26/50 (52%), Positives = 30/50 (60%), Gaps = 1/50 (2%)
Frame = +2
Query: 416 EEVVDFLKKYDFDGLDLDWEYPGAADRGGS-FSDKDEFLYFVQELKRAFI 562
+ V+ L+ + FDGLDLDWEYP A RGGS DK F QEL FI
Sbjct: 121 DSVIRILRTWGFDGLDLDWEYP--ATRGGSPPEDKQRFTVLCQELMNTFI 168
>SB_25657| Best HMM Match : Glyco_hydro_18 (HMM E-Value=0)
Length = 829
Score = 76.6 bits (180), Expect = 2e-14
Identities = 49/130 (37%), Positives = 69/130 (53%), Gaps = 2/130 (1%)
Frame = +1
Query: 52 LAVLASCAALVQCADSRARIVCYFSNWAVYRPGVGRYGIEDIPVDLCTHLIYSFIGVTEK 231
LA+L C V A S VCY++NW+ YRP G + EDI LCTH+I+SF V
Sbjct: 391 LAILILC--FVPFAVSEYVRVCYYTNWSQYRPKGGTFWPEDIDPHLCTHVIHSFSKV-NL 447
Query: 232 SSEVLIIDPELDVDKSGFRNFTSLRSKHPDVKFMVAVGGWA--EGGSKYSHMVAQKSTRM 405
++ V+ + D D ++ +L+ +P +K +AVGGW E S +S MVA K R
Sbjct: 448 TTHVMEKYEKNDFDL--YKRINALKKINPKLKTQIAVGGWTHEEKNSPFSKMVATKEKRA 505
Query: 406 SFIRRGCRLL 435
FI+ L
Sbjct: 506 IFIKSAIETL 515
Score = 75.8 bits (178), Expect = 3e-14
Identities = 39/109 (35%), Positives = 61/109 (55%), Gaps = 2/109 (1%)
Frame = +1
Query: 115 CYFSNWAVYRPGVGRYGIEDIPVDLCTHLIYSFIGVTEKSSEVLIIDPELDVDKSGFRNF 294
CY++NW+ YRP G + EDI LCTH+++SF V + + ++ I + + D ++
Sbjct: 25 CYYTNWSQYRPKGGTFWPEDIDPFLCTHILFSFAKVNQTTHKLDIYE---ENDHELYQRI 81
Query: 295 TSLRSKHPDVKFMVAVGGWA--EGGSKYSHMVAQKSTRMSFIRRGCRLL 435
+L+ +P +K +AVGGW E S +S MVA K R FI+ L
Sbjct: 82 NALKKINPKLKTQIAVGGWTHEEKNSPFSKMVATKEKRAIFIKSAIETL 130
Score = 72.1 bits (169), Expect = 4e-13
Identities = 44/101 (43%), Positives = 57/101 (56%), Gaps = 6/101 (5%)
Frame = +2
Query: 416 EEVVDFLKKYDFDGLDLDWEYPGAADRGGS-FSDKDEFLYFVQELKRAF--IRADRGWE- 583
+ ++ L+ FDGLDLDWEYPG RGGS SDK F QEL+ AF D G E
Sbjct: 509 KSAIETLRTNGFDGLDLDWEYPGM--RGGSPKSDKGRFTLLCQELRDAFEAEAKDSGKER 566
Query: 584 --LTAAVPLANFRLMEGYHVPELCQELDAIHVMSYDLRXNW 700
LTAAV + + + Y + + + LD I+VM+YDL W
Sbjct: 567 LLLTAAVAAGLWTIKDAYDIEGISKPLDWINVMTYDLHGTW 607
Score = 70.5 bits (165), Expect = 1e-12
Identities = 41/101 (40%), Positives = 58/101 (57%), Gaps = 6/101 (5%)
Frame = +2
Query: 416 EEVVDFLKKYDFDGLDLDWEYPGAADRGGS-FSDKDEFLYFVQELKRAFIRADR--GWE- 583
+ ++ L+ + FDGLDLDWEYPG RGGS SDK F +EL+ AF + G E
Sbjct: 124 KSAIETLRTHGFDGLDLDWEYPGM--RGGSPKSDKGRFTLLCRELREAFEAEAKASGKER 181
Query: 584 --LTAAVPLANFRLMEGYHVPELCQELDAIHVMSYDLRXNW 700
LTAAV + + Y + ++ + LD I++M+YDL W
Sbjct: 182 MLLTAAVAAGLWTIKGAYDIADIAEPLDWINLMTYDLHGTW 222
>SB_38742| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 438
Score = 76.2 bits (179), Expect = 3e-14
Identities = 46/108 (42%), Positives = 58/108 (53%), Gaps = 5/108 (4%)
Frame = +2
Query: 416 EEVVDFLKKYDFDGLDLDWEYPGAADRGGSFSDKDEFLYFVQELKRAF--IRADRGWE-- 583
++ + FL+ + FDGLDLDWEYPGA RG DK F QEL AF A+ G
Sbjct: 121 DDAILFLRNHMFDGLDLDWEYPGA--RGSPPEDKHRFTLLCQELLAAFETEAANTGKPRL 178
Query: 584 -LTAAVPLANFRLMEGYHVPELCQELDAIHVMSYDLRXNWAGFADVHS 724
LTAAV + GY + L Q LD I++MSYDL +W H+
Sbjct: 179 LLTAAVSAGETTVKNGYEISALGQALDWINLMSYDLHGDWEDTTGHHA 226
Score = 68.5 bits (160), Expect = 5e-12
Identities = 34/101 (33%), Positives = 59/101 (58%)
Frame = +1
Query: 112 VCYFSNWAVYRPGVGRYGIEDIPVDLCTHLIYSFIGVTEKSSEVLIIDPELDVDKSGFRN 291
VCY++NWA YR G+ +Y ++I LCTH++Y+F + +S++ + + D + ++
Sbjct: 25 VCYYTNWAQYR-GLAKYTPDNIDPSLCTHIVYAFAKMNADNSKLAMFEWN---DAAMYKK 80
Query: 292 FTSLRSKHPDVKFMVAVGGWAEGGSKYSHMVAQKSTRMSFI 414
L+S ++K ++AVGGW G + MV+ S R FI
Sbjct: 81 VNDLKSS-SNMKTLLAVGGWNMGSGPFQDMVSTASRRKIFI 120
>SB_24154| Best HMM Match : Glyco_hydro_18 (HMM E-Value=2.2e-32)
Length = 561
Score = 76.2 bits (179), Expect = 3e-14
Identities = 46/108 (42%), Positives = 58/108 (53%), Gaps = 5/108 (4%)
Frame = +2
Query: 416 EEVVDFLKKYDFDGLDLDWEYPGAADRGGSFSDKDEFLYFVQELKRAF--IRADRGWE-- 583
++ + FL+ + FDGLDLDWEYPGA RG DK F QEL AF A+ G
Sbjct: 45 DDAILFLRNHMFDGLDLDWEYPGA--RGSPPEDKHRFTLLCQELLAAFETEAANTGKPRL 102
Query: 584 -LTAAVPLANFRLMEGYHVPELCQELDAIHVMSYDLRXNWAGFADVHS 724
LTAAV + GY + L Q LD I++MSYDL +W H+
Sbjct: 103 LLTAAVSAGETTVKNGYEISALGQALDWINLMSYDLHGDWEDTTGHHA 150
Score = 31.1 bits (67), Expect = 0.96
Identities = 15/44 (34%), Positives = 24/44 (54%)
Frame = +1
Query: 283 FRNFTSLRSKHPDVKFMVAVGGWAEGGSKYSHMVAQKSTRMSFI 414
++ L+S ++K ++AVGGW G + MV+ S R FI
Sbjct: 2 YKKVNDLKSSS-NMKTLLAVGGWNMGSGPFQDMVSTASRRKIFI 44
>SB_28916| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 807
Score = 75.8 bits (178), Expect = 3e-14
Identities = 40/104 (38%), Positives = 62/104 (59%), Gaps = 3/104 (2%)
Frame = +1
Query: 112 VCYFSNWAVYRPGVGRYGIEDIPVDLCTHLIYSFIGVTEKSSEVLIIDPELDVDKSGFRN 291
VCYF+NWA YRP ++ +DI LCTH++Y+F + ++++ + D ++
Sbjct: 401 VCYFTNWAQYRPDPVKFLPKDIDPLLCTHIVYAFAKIDPATNKIGTYEWN---DDRLYKE 457
Query: 292 FTSLRSKHPDVKFMVAVGGW-AEGG--SKYSHMVAQKSTRMSFI 414
L+ K+P +K ++AVGGW E G S +S MVA KS R +FI
Sbjct: 458 INDLKLKNPSLKTLLAVGGWNHESGPVSPFSQMVASKSNRTTFI 501
Score = 64.9 bits (151), Expect = 6e-11
Identities = 43/101 (42%), Positives = 53/101 (52%), Gaps = 6/101 (5%)
Frame = +2
Query: 440 KYDFDGLDLDWEYPGAADRGGS-FSDKDEFLYFVQELKRAFIR--ADRG---WELTAAVP 601
KYDFDG DLDWEYP A RG S DK F +E+ AF R AD LTAAV
Sbjct: 510 KYDFDGFDLDWEYP--ASRGNSPPQDKQHFTILCEEMLDAFKRKAADTDKPRMLLTAAVS 567
Query: 602 LANFRLMEGYHVPELCQELDAIHVMSYDLRXNWAGFADVHS 724
+ + Y V +L LD I++M+YDL W + H+
Sbjct: 568 AGHGTVDAAYEVHKLAGILDWINLMTYDLHGPWEPYTGHHT 608
>SB_48525| Best HMM Match : Glyco_hydro_18 (HMM E-Value=0)
Length = 569
Score = 64.9 bits (151), Expect = 6e-11
Identities = 41/98 (41%), Positives = 56/98 (57%), Gaps = 6/98 (6%)
Frame = +2
Query: 425 VDFLKKYDFDGLDLDWEYPGAADRGGSF-SDKDEFLYFVQELKRAF----IRADR-GWEL 586
+ L++++FDG DLDWEYPG +R GS DK F QEL AF + +R L
Sbjct: 236 IAMLRQWNFDGFDLDWEYPG--NRVGSDPGDKQRFTILCQELLDAFKLEAAQTNRPRLLL 293
Query: 587 TAAVPLANFRLMEGYHVPELCQELDAIHVMSYDLRXNW 700
TAAV + + Y V ++ Q LD I++M+YDLR W
Sbjct: 294 TAAVAAGKATIDKAYEVDKIGQILDLINLMAYDLRGMW 331
Score = 37.1 bits (82), Expect = 0.015
Identities = 19/59 (32%), Positives = 31/59 (52%), Gaps = 2/59 (3%)
Frame = +1
Query: 271 DKSGFRNFTSLRSKHPDVKFMVAVGGWAEGG--SKYSHMVAQKSTRMSFIRRGCRLLEE 441
D ++ F L+ K+P +K ++AVGGW S +S MV ++R FI +L +
Sbjct: 183 DDKLYKEFNDLKLKNPGLKTLLAVGGWNHENYKSPFSRMVRTAASRKVFIDSSIAMLRQ 241
>SB_17224| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 935
Score = 32.3 bits (70), Expect = 0.41
Identities = 28/100 (28%), Positives = 48/100 (48%), Gaps = 9/100 (9%)
Frame = +1
Query: 46 ATLAVLASCAALVQCADSRARIVCYFSNWAVYRPGVGRYGIEDIPVDLC--THLIYSFIG 219
A LA + + A + A SR ++ +++ Y GV GI + D C TH +Y+++
Sbjct: 485 AKLAYIGAGALAIGYAASR--LLSAYTHAYTYAEGVAD-GIRLLETDTCVATHRVYAYVY 541
Query: 220 VTEKSSEVLIIDPELDV-------DKSGFRNFTSLRSKHP 318
V + + + D ELD + GF+ T+L +K P
Sbjct: 542 VMARRNGKRMSDEELDAFLRPIYYARGGFQGVTALHAKLP 581
>SB_47830| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 767
Score = 30.3 bits (65), Expect = 1.7
Identities = 12/27 (44%), Positives = 19/27 (70%)
Frame = +2
Query: 413 SEEVVDFLKKYDFDGLDLDWEYPGAAD 493
+EE++DFLKK DF LD+ + P ++
Sbjct: 347 NEEIIDFLKKSDFGRLDVGFTDPRCSE 373
>SB_12527| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 365
Score = 29.5 bits (63), Expect = 2.9
Identities = 22/61 (36%), Positives = 28/61 (45%), Gaps = 1/61 (1%)
Frame = +2
Query: 344 AVGLKAAPSTH-TWWPRRALGCPSSEEVVDFLKKYDFDGLDLDWEYPGAADRGGSFSDKD 520
AV +A P H W P R L E + F GL +DWE A+RGG ++D
Sbjct: 153 AVKQQALPERHEAWSPYRPL---KVERLFAF-------GLSMDWEKLADAERGGERGERD 202
Query: 521 E 523
E
Sbjct: 203 E 203
>SB_27365| Best HMM Match : PID (HMM E-Value=1.90001e-40)
Length = 837
Score = 28.3 bits (60), Expect = 6.7
Identities = 21/62 (33%), Positives = 30/62 (48%), Gaps = 2/62 (3%)
Frame = +2
Query: 308 PNTPMSSSWSRLAVGLKAAPSTHTWWPRRALGCP--SSEEVVDFLKKYDFDGLDLDWEYP 481
PNT S++ +L+ A PST+T ++ P S V F +D D LDL + P
Sbjct: 332 PNTTNSTTSDQLSSLFDAQPSTYTTNSTSSMTPPYDQSPSVNPFSSPFDSDSLDLLDQKP 391
Query: 482 GA 487
A
Sbjct: 392 TA 393
>SB_46249| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 834
Score = 27.9 bits (59), Expect = 8.9
Identities = 25/67 (37%), Positives = 37/67 (55%), Gaps = 5/67 (7%)
Frame = -2
Query: 660 SSS*HSSGTWYPSISLKLASGTAAVSSHPL---SALM--NALFNSWTKYKNSSLSEKEPP 496
SSS + S + Y S SL +S + S PL S+L ++L+ S + YK+SSL E P
Sbjct: 260 SSSLYKSSSLYKSSSLYKSS--SLYESPPLYKSSSLYKSSSLYKSSSLYKSSSLYESPPL 317
Query: 495 RSAAPGY 475
++P Y
Sbjct: 318 YKSSPLY 324
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,529,970
Number of Sequences: 59808
Number of extensions: 506197
Number of successful extensions: 1415
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1248
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1397
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1949964354
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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