Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0774
(750 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g20920.2 68418.m02485 eukaryotic translation initiation facto... 149 2e-36
At5g20920.1 68418.m02484 eukaryotic translation initiation facto... 149 2e-36
At3g07920.1 68416.m00967 eukaryotic translation initiation facto... 100 2e-21
At5g01940.1 68418.m00113 eukaryotic translation initiation facto... 83 1e-16
At5g44280.1 68418.m05420 zinc finger (C3HC4-type RING finger) fa... 29 4.4
At3g42100.1 68416.m04322 AT hook motif-containing protein-relate... 29 4.4
At2g43710.2 68415.m05434 acyl-[acyl-carrier-protein] desaturase ... 29 4.4
At2g43710.1 68415.m05433 acyl-[acyl-carrier-protein] desaturase ... 29 4.4
At5g16240.1 68418.m01897 acyl-[acyl-carrier-protein] desaturase,... 28 5.8
At2g35690.1 68415.m04377 acyl-CoA oxidase, putative strong simil... 28 5.8
>At5g20920.2 68418.m02485 eukaryotic translation initiation factor 2
subunit 2, putative / eIF-2-beta, putative similar to
SP|P41035 Eukaryotic translation initiation factor 2
subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains
Pfam profile PF01873: Domain found in IF2B/IF5
Length = 267
Score = 149 bits (361), Expect = 2e-36
Identities = 67/130 (51%), Positives = 94/130 (72%), Gaps = 4/130 (3%)
Frame = +1
Query: 355 EDVRGEDQENVE----DEHGEWVGSDHDYSYDELLERVFDIMREKNPSMVSGKKQKFIMR 522
+++ ++QE E + W GS+ DY YDELL RVF+I+RE NP + +G +++ +MR
Sbjct: 87 DEIANDEQEGEEGIVLQQRYPWEGSERDYIYDELLGRVFNILRENNPEL-AGDRRRTVMR 145
Query: 523 PPQVVRIGTKKTSFANFTEICKTLHRQAKHLLDFLLAELGTSGSVDGNSQLIIKGRXQQK 702
PPQV+R GTKKT F NF ++CKT+HRQ H++ +LLAELGTSGS+DG +L++KGR K
Sbjct: 146 PPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQRLVVKGRFAPK 205
Query: 703 QIENVLRRYI 732
E +LRRYI
Sbjct: 206 NFEGILRRYI 215
>At5g20920.1 68418.m02484 eukaryotic translation initiation factor 2
subunit 2, putative / eIF-2-beta, putative similar to
SP|P41035 Eukaryotic translation initiation factor 2
subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains
Pfam profile PF01873: Domain found in IF2B/IF5
Length = 268
Score = 149 bits (361), Expect = 2e-36
Identities = 67/130 (51%), Positives = 94/130 (72%), Gaps = 4/130 (3%)
Frame = +1
Query: 355 EDVRGEDQENVE----DEHGEWVGSDHDYSYDELLERVFDIMREKNPSMVSGKKQKFIMR 522
+++ ++QE E + W GS+ DY YDELL RVF+I+RE NP + +G +++ +MR
Sbjct: 88 DEIANDEQEGEEGIVLQQRYPWEGSERDYIYDELLGRVFNILRENNPEL-AGDRRRTVMR 146
Query: 523 PPQVVRIGTKKTSFANFTEICKTLHRQAKHLLDFLLAELGTSGSVDGNSQLIIKGRXQQK 702
PPQV+R GTKKT F NF ++CKT+HRQ H++ +LLAELGTSGS+DG +L++KGR K
Sbjct: 147 PPQVLREGTKKTVFVNFMDLCKTMHRQPDHVMQYLLAELGTSGSLDGQQRLVVKGRFAPK 206
Query: 703 QIENVLRRYI 732
E +LRRYI
Sbjct: 207 NFEGILRRYI 216
>At3g07920.1 68416.m00967 eukaryotic translation initiation factor 2
subunit 2, putative / eIF-2-beta, putative similar to
SP|P41035 Eukaryotic translation initiation factor 2
subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains
Pfam profile PF01873: Domain found in IF2B/IF5
Length = 169
Score = 99.5 bits (237), Expect = 2e-21
Identities = 50/102 (49%), Positives = 69/102 (67%), Gaps = 4/102 (3%)
Frame = +1
Query: 439 ELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRI----GTKKTSFANFTEICKTLHRQA 606
E+L RVF+I+RE +P +V G I+ PPQV+R GTKKT F NF + CKT+HR
Sbjct: 18 EILRRVFNILRENSPELV-GIWLLTIIWPPQVLREETAKGTKKTVFVNFMDYCKTMHRNP 76
Query: 607 KHLLDFLLAELGTSGSVDGNSQLIIKGRXQQKQIENVLRRYI 732
H++ FLLAELGT G +D +L+++GR Q+ E++LR YI
Sbjct: 77 DHVMAFLLAELGTRGKLDDQQRLVVRGRFTQRDFESLLRGYI 118
>At5g01940.1 68418.m00113 eukaryotic translation initiation factor
2B family protein / eIF-2B family protein similar to
SP|P41035 Eukaryotic translation initiation factor 2
subunit (eIF-2-beta) {Oryctolagus cuniculus}; contains
Pfam profile PF01873: Domain found in IF2B/IF5
Length = 231
Score = 83.4 bits (197), Expect = 1e-16
Identities = 42/103 (40%), Positives = 65/103 (63%)
Frame = +1
Query: 424 DYSYDELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKTSFANFTEICKTLHRQ 603
DY Y ELL VFD +RE++ VS ++ + +M PPQ++ GT T NF ++C+T+HR+
Sbjct: 72 DYGYKELLSMVFDRLREEDVE-VSTERPRTVMMPPQLLAEGTI-TVCLNFADLCRTMHRK 129
Query: 604 AKHLLDFLLAELGTSGSVDGNSQLIIKGRXQQKQIENVLRRYI 732
H++ FLLA++ T S++ +L IKG K + V R+YI
Sbjct: 130 PDHVMKFLLAQMETKVSLNKQQRLEIKGLVSSKDFQAVFRKYI 172
>At5g44280.1 68418.m05420 zinc finger (C3HC4-type RING finger)
family protein contains similarity to polycomb-M33
interacting protein Ring1B [Mus musculus] GI:2239142;
contains Pfam profile PF00097: Zinc finger, C3HC4 type
(RING finger)
Length = 486
Score = 28.7 bits (61), Expect = 4.4
Identities = 14/54 (25%), Positives = 29/54 (53%)
Frame = +1
Query: 355 EDVRGEDQENVEDEHGEWVGSDHDYSYDELLERVFDIMREKNPSMVSGKKQKFI 516
+D +++E VE E E + + +E E + +E++PS +SG + +F+
Sbjct: 72 DDAEEDEEEEVEAEEDEEAEEEEEEEEEE--EEEEEDSKERSPSSISGDQSEFM 123
>At3g42100.1 68416.m04322 AT hook motif-containing protein-related
very low similarity to SP|Q9UUA2 DNA repair and
recombination protein pif1, mitochondrial precursor
{Schizosaccharomyces pombe}; weak hit to Pfam profile
PF02178: AT hook motif
Length = 1752
Score = 28.7 bits (61), Expect = 4.4
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = -2
Query: 662 PSTEPLVPNSARRKSNKCLACR 597
P E +PNSA++K KC++ R
Sbjct: 590 PGIEKCLPNSAKKKKKKCISMR 611
>At2g43710.2 68415.m05434 acyl-[acyl-carrier-protein] desaturase /
stearoyl-ACP desaturase (SSI2) identical to gi:15149310;
contains Pfam profile PF03405: Fatty acid desaturase;
identical to cDNA stearoyl ACP desaturase (SSI2),
SSI2-FAB2 allele, GI:15149309
Length = 401
Score = 28.7 bits (61), Expect = 4.4
Identities = 13/58 (22%), Positives = 29/58 (50%)
Frame = +1
Query: 421 HDYSYDELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKTSFANFTEICKTL 594
H+ +Y +++E++F+I + + +K I P ++ G F NF+ + + L
Sbjct: 270 HETAYTKIVEKLFEIDPDGTVMAFADMMRKKISMPAHLMYDGRNDNLFDNFSSVAQRL 327
>At2g43710.1 68415.m05433 acyl-[acyl-carrier-protein] desaturase /
stearoyl-ACP desaturase (SSI2) identical to gi:15149310;
contains Pfam profile PF03405: Fatty acid desaturase;
identical to cDNA stearoyl ACP desaturase (SSI2),
SSI2-FAB2 allele, GI:15149309
Length = 401
Score = 28.7 bits (61), Expect = 4.4
Identities = 13/58 (22%), Positives = 29/58 (50%)
Frame = +1
Query: 421 HDYSYDELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKTSFANFTEICKTL 594
H+ +Y +++E++F+I + + +K I P ++ G F NF+ + + L
Sbjct: 270 HETAYTKIVEKLFEIDPDGTVMAFADMMRKKISMPAHLMYDGRNDNLFDNFSSVAQRL 327
>At5g16240.1 68418.m01897 acyl-[acyl-carrier-protein] desaturase,
putative / stearoyl-ACP desaturase, putative similar to
Acyl-[acyl-carrier protein] desaturase from Sesamum
indicum GI:575942, Cucumis sativus SP|P32061, Ricinus
communis SP|P22337; contains Pfam profile PF03405 Fatty
acid desaturase
Length = 394
Score = 28.3 bits (60), Expect = 5.8
Identities = 22/94 (23%), Positives = 44/94 (46%), Gaps = 1/94 (1%)
Frame = +1
Query: 421 HDYSYDELLERVFDIMREKNPSMVSGKKQKFIMRPPQVVRIGTKKTSFANFTEICKTLH- 597
H+++Y +++E++F+I + + +K I P ++ G F +F+ + + L
Sbjct: 262 HEHAYTKIVEKLFEIDPDTTVVGFADMMRKKISMPAHLMYDGRDDNLFDHFSSVAQRLGV 321
Query: 598 RQAKHLLDFLLAELGTSGSVDGNSQLIIKGRXQQ 699
AK D +L L +V+ S L +G Q
Sbjct: 322 YTAKDYAD-ILQHLVERWNVEKLSDLSSEGNRAQ 354
>At2g35690.1 68415.m04377 acyl-CoA oxidase, putative strong
similarity to acyl-CoA oxidase [Arabidopsis thaliana]
GI:3044214
Length = 664
Score = 28.3 bits (60), Expect = 5.8
Identities = 16/54 (29%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
Frame = +1
Query: 358 DVRGEDQENVEDEHGEWVGSDHDYSYDELLERVF--DIMREKNPSMVSGKKQKF 513
D R + + NV+D W GS H + + R+ D + EK+ V +K+ F
Sbjct: 9 DERNKAEFNVDDMKIVWAGSRHAFDVSNRMSRLVANDPVFEKSKRAVMSRKELF 62
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,603,055
Number of Sequences: 28952
Number of extensions: 235910
Number of successful extensions: 934
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 880
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 928
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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