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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0754
         (750 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g32190.1 68417.m04581 centromeric protein-related low similar...    29   0.36 
At1g48070.1 68414.m05360 expressed protein                             31   0.82 
At3g29390.1 68416.m03693 hydroxyproline-rich glycoprotein family...    30   1.4  
At3g54850.1 68416.m06077 armadillo/beta-catenin repeat family pr...    30   1.9  
At5g11120.1 68418.m01298 hypothetical protein                          29   3.3  
At4g16700.1 68417.m02523 phosphatidylserine decarboxylase simila...    29   4.4  
At4g31480.1 68417.m04472 coatomer beta subunit, putative / beta-...    28   7.6  
At3g52670.1 68416.m05802 F-box family protein contains F-box dom...    28   7.6  

>At4g32190.1 68417.m04581 centromeric protein-related low similarity
           to SP|Q02224 Centromeric protein E (CENP-E protein)
           {Homo sapiens}
          Length = 783

 Score = 29.1 bits (62), Expect(2) = 0.36
 Identities = 21/77 (27%), Positives = 38/77 (49%)
 Frame = +1

Query: 265 QKVILLKNRLEKLMKADNKEKIILFTDSYDVMFLGNLDEIVKKFKSFPDTRVLFSAEQFC 444
           +++ + K  LE L +   KEK +L    +D+  +   DE+ KK  +F  ++ L   ++  
Sbjct: 406 REISVQKELLEDLREELQKEKPLLELAMHDISVIQ--DELYKKANAFQVSQNLLQEKESS 463

Query: 445 WPDAKLATQYPNIEVVS 495
             +AKL  Q+   E  S
Sbjct: 464 LVEAKLEIQHLKSEQAS 480



 Score = 21.8 bits (44), Expect(2) = 0.36
 Identities = 12/34 (35%), Positives = 16/34 (47%)
 Frame = +1

Query: 67  ILCVFVILFNSHTQATDEVVVLTVATDDNHGLER 168
           +L     L ++HT+   E V L V    N  LER
Sbjct: 373 VLSYMQSLRDAHTEVESERVKLRVVEAKNFALER 406


>At1g48070.1 68414.m05360 expressed protein
          Length = 114

 Score = 31.1 bits (67), Expect = 0.82
 Identities = 10/20 (50%), Positives = 14/20 (70%)
 Frame = -2

Query: 269 FCPPPGKFMSPPDHSSPLPN 210
           F PPP  F+ PP  S+P+P+
Sbjct: 92  FAPPPSDFVQPPSDSAPVPS 111


>At3g29390.1 68416.m03693 hydroxyproline-rich glycoprotein family
           protein sequencing discrepancy between cDNA and genomic
           sequence prevents representation of entire coding
           sequence
          Length = 578

 Score = 30.3 bits (65), Expect = 1.4
 Identities = 15/39 (38%), Positives = 18/39 (46%)
 Frame = -2

Query: 272 TFCPPPGKFMSPPDHSSPLPNTSTSILYTLAERKNLSRP 156
           T  PPP K MSPP   S LP    S   +    K++  P
Sbjct: 486 TIAPPPSKTMSPPSSKSMLPPPPRSKTMSPLSSKSMLPP 524


>At3g54850.1 68416.m06077 armadillo/beta-catenin repeat family
           protein / U-box domain-containing family protein
           contains Pfam domain, PF00514:
           Armadillo/beta-catenin-like repeats and Pfam, PF04564:
           U-box domain
          Length = 632

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 21/91 (23%), Positives = 45/91 (49%), Gaps = 3/91 (3%)
 Frame = +1

Query: 442 CWPDAKLATQYPNIEVVSPYLNSGGFIGYLPEIYEIINSNPIKDKDDDQLYYTKIYLDKD 621
           C  +  ++    +++ +S + +S GFIG +    +++    +     ++L    + L KD
Sbjct: 7   CSHEELMSRLVDSVKEISGFSSSRGFIGKIQG--DLVRRITLLSPFFEELIDVNVELKKD 64

Query: 622 LR---ESLKITLDHKSEIFQNLNGALSDVQL 705
                E+++I LD   E+F+++NG     QL
Sbjct: 65  QITGFEAMRIALDSSLELFRSVNGGSKLFQL 95


>At5g11120.1 68418.m01298 hypothetical protein 
          Length = 162

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = -3

Query: 316 CQLSSASQACFSEELLFVHHRGSSCHHLTT 227
           C+LSS+ QA FS   +F     SSC ++T+
Sbjct: 42  CRLSSSPQAVFSSFRIFPFRSSSSCLNITS 71


>At4g16700.1 68417.m02523 phosphatidylserine decarboxylase similar
           to SP|P27465 Phosphatidylserine decarboxylase proenzyme
           (EC 4.1.1.65 {Cricetulus griseus}; contains Pfam profile
           PF02666: phosphatidylserine decarboxylase
          Length = 453

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 20/71 (28%), Positives = 33/71 (46%)
 Frame = +1

Query: 532 PEIYEIINSNPIKDKDDDQLYYTKIYLDKDLRESLKITLDHKSEIFQNLNGALSDVQLRA 711
           P++ E ++++P+K      LYY  IYL       +    D  + + ++  G L  V  RA
Sbjct: 266 PKLRENVSASPMKG-----LYYCVIYLKPGDYHRIHSPADWNATVRRHFAGRLFPVNERA 320

Query: 712 NTTEEWPYIEN 744
             T    Y+EN
Sbjct: 321 TRTIRNLYVEN 331


>At4g31480.1 68417.m04472 coatomer beta subunit, putative /
           beta-coat protein, putative / beta-COP, putative similar
           to Coatomer beta subunit (Beta-coat protein) (Beta-COP)
           from {Rattus norvegicus} SP|P23514, {Mus musculus}
           SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam
           profile: PF01602 Adaptin N terminal region
          Length = 971

 Score = 27.9 bits (59), Expect = 7.6
 Identities = 13/39 (33%), Positives = 24/39 (61%), Gaps = 2/39 (5%)
 Frame = -3

Query: 508 SSNMDLQLQCLDIELQVL--HQANKIVQQKKAHVYQEKT 398
           S N+D++ + LDI L ++  H  N++VQ  K  V + ++
Sbjct: 350 SPNLDIRRKTLDISLDLITHHNINEVVQMLKKEVVKTQS 388


>At3g52670.1 68416.m05802 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 384

 Score = 27.9 bits (59), Expect = 7.6
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = +3

Query: 12  RTYIYDVVHSKNGTFYTINIMCICY 86
           R  I DV+H K    YTIN+ C+ Y
Sbjct: 175 RLEICDVLHKKEFRRYTINVPCLKY 199


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,216,597
Number of Sequences: 28952
Number of extensions: 348164
Number of successful extensions: 1134
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 1039
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1133
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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