BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0754 (750 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g32190.1 68417.m04581 centromeric protein-related low similar... 29 0.36 At1g48070.1 68414.m05360 expressed protein 31 0.82 At3g29390.1 68416.m03693 hydroxyproline-rich glycoprotein family... 30 1.4 At3g54850.1 68416.m06077 armadillo/beta-catenin repeat family pr... 30 1.9 At5g11120.1 68418.m01298 hypothetical protein 29 3.3 At4g16700.1 68417.m02523 phosphatidylserine decarboxylase simila... 29 4.4 At4g31480.1 68417.m04472 coatomer beta subunit, putative / beta-... 28 7.6 At3g52670.1 68416.m05802 F-box family protein contains F-box dom... 28 7.6 >At4g32190.1 68417.m04581 centromeric protein-related low similarity to SP|Q02224 Centromeric protein E (CENP-E protein) {Homo sapiens} Length = 783 Score = 29.1 bits (62), Expect(2) = 0.36 Identities = 21/77 (27%), Positives = 38/77 (49%) Frame = +1 Query: 265 QKVILLKNRLEKLMKADNKEKIILFTDSYDVMFLGNLDEIVKKFKSFPDTRVLFSAEQFC 444 +++ + K LE L + KEK +L +D+ + DE+ KK +F ++ L ++ Sbjct: 406 REISVQKELLEDLREELQKEKPLLELAMHDISVIQ--DELYKKANAFQVSQNLLQEKESS 463 Query: 445 WPDAKLATQYPNIEVVS 495 +AKL Q+ E S Sbjct: 464 LVEAKLEIQHLKSEQAS 480 Score = 21.8 bits (44), Expect(2) = 0.36 Identities = 12/34 (35%), Positives = 16/34 (47%) Frame = +1 Query: 67 ILCVFVILFNSHTQATDEVVVLTVATDDNHGLER 168 +L L ++HT+ E V L V N LER Sbjct: 373 VLSYMQSLRDAHTEVESERVKLRVVEAKNFALER 406 >At1g48070.1 68414.m05360 expressed protein Length = 114 Score = 31.1 bits (67), Expect = 0.82 Identities = 10/20 (50%), Positives = 14/20 (70%) Frame = -2 Query: 269 FCPPPGKFMSPPDHSSPLPN 210 F PPP F+ PP S+P+P+ Sbjct: 92 FAPPPSDFVQPPSDSAPVPS 111 >At3g29390.1 68416.m03693 hydroxyproline-rich glycoprotein family protein sequencing discrepancy between cDNA and genomic sequence prevents representation of entire coding sequence Length = 578 Score = 30.3 bits (65), Expect = 1.4 Identities = 15/39 (38%), Positives = 18/39 (46%) Frame = -2 Query: 272 TFCPPPGKFMSPPDHSSPLPNTSTSILYTLAERKNLSRP 156 T PPP K MSPP S LP S + K++ P Sbjct: 486 TIAPPPSKTMSPPSSKSMLPPPPRSKTMSPLSSKSMLPP 524 >At3g54850.1 68416.m06077 armadillo/beta-catenin repeat family protein / U-box domain-containing family protein contains Pfam domain, PF00514: Armadillo/beta-catenin-like repeats and Pfam, PF04564: U-box domain Length = 632 Score = 29.9 bits (64), Expect = 1.9 Identities = 21/91 (23%), Positives = 45/91 (49%), Gaps = 3/91 (3%) Frame = +1 Query: 442 CWPDAKLATQYPNIEVVSPYLNSGGFIGYLPEIYEIINSNPIKDKDDDQLYYTKIYLDKD 621 C + ++ +++ +S + +S GFIG + +++ + ++L + L KD Sbjct: 7 CSHEELMSRLVDSVKEISGFSSSRGFIGKIQG--DLVRRITLLSPFFEELIDVNVELKKD 64 Query: 622 LR---ESLKITLDHKSEIFQNLNGALSDVQL 705 E+++I LD E+F+++NG QL Sbjct: 65 QITGFEAMRIALDSSLELFRSVNGGSKLFQL 95 >At5g11120.1 68418.m01298 hypothetical protein Length = 162 Score = 29.1 bits (62), Expect = 3.3 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = -3 Query: 316 CQLSSASQACFSEELLFVHHRGSSCHHLTT 227 C+LSS+ QA FS +F SSC ++T+ Sbjct: 42 CRLSSSPQAVFSSFRIFPFRSSSSCLNITS 71 >At4g16700.1 68417.m02523 phosphatidylserine decarboxylase similar to SP|P27465 Phosphatidylserine decarboxylase proenzyme (EC 4.1.1.65 {Cricetulus griseus}; contains Pfam profile PF02666: phosphatidylserine decarboxylase Length = 453 Score = 28.7 bits (61), Expect = 4.4 Identities = 20/71 (28%), Positives = 33/71 (46%) Frame = +1 Query: 532 PEIYEIINSNPIKDKDDDQLYYTKIYLDKDLRESLKITLDHKSEIFQNLNGALSDVQLRA 711 P++ E ++++P+K LYY IYL + D + + ++ G L V RA Sbjct: 266 PKLRENVSASPMKG-----LYYCVIYLKPGDYHRIHSPADWNATVRRHFAGRLFPVNERA 320 Query: 712 NTTEEWPYIEN 744 T Y+EN Sbjct: 321 TRTIRNLYVEN 331 >At4g31480.1 68417.m04472 coatomer beta subunit, putative / beta-coat protein, putative / beta-COP, putative similar to Coatomer beta subunit (Beta-coat protein) (Beta-COP) from {Rattus norvegicus} SP|P23514, {Mus musculus} SP|Q9JIF7, {Homo sapiens} SP|P53618; contains Pfam profile: PF01602 Adaptin N terminal region Length = 971 Score = 27.9 bits (59), Expect = 7.6 Identities = 13/39 (33%), Positives = 24/39 (61%), Gaps = 2/39 (5%) Frame = -3 Query: 508 SSNMDLQLQCLDIELQVL--HQANKIVQQKKAHVYQEKT 398 S N+D++ + LDI L ++ H N++VQ K V + ++ Sbjct: 350 SPNLDIRRKTLDISLDLITHHNINEVVQMLKKEVVKTQS 388 >At3g52670.1 68416.m05802 F-box family protein contains F-box domain Pfam:PF00646 Length = 384 Score = 27.9 bits (59), Expect = 7.6 Identities = 12/25 (48%), Positives = 15/25 (60%) Frame = +3 Query: 12 RTYIYDVVHSKNGTFYTINIMCICY 86 R I DV+H K YTIN+ C+ Y Sbjct: 175 RLEICDVLHKKEFRRYTINVPCLKY 199 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,216,597 Number of Sequences: 28952 Number of extensions: 348164 Number of successful extensions: 1134 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 1039 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1133 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1663169840 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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