Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0712
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 196 3e-51
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 132 6e-32
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 109 5e-25
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 107 2e-24
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 102 4e-23
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 97 2e-21
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 77 3e-15
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 75 7e-15
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 73 5e-14
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 72 9e-14
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 71 2e-13
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 64 2e-11
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 54 1e-08
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 28 1.5
SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch... 27 2.6
SPCC757.11c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 7.9
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 196 bits (478), Expect = 3e-51
Identities = 88/147 (59%), Positives = 108/147 (73%)
Frame = +1
Query: 166 ALPRRIIKETQRLMQEPVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDY 345
ALP+RIIKE + L ++P PGI A P+E N RYF + + GP+ S +Y
Sbjct: 2 ALPKRIIKEIETLTRDPPPGIVAAPTEDNLRYFKITMEGPQQSAYEGGKFHLELFLPDEY 61
Query: 346 PMSAPKVRFITKIYHPNIDRLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLA 525
PM P VRF+TKIYHPN+D+LGRICL LK WSPALQIRTVLLSIQAL+ APNPDDPL
Sbjct: 62 PMMPPNVRFLTKIYHPNVDKLGRICLSTLKKDWSPALQIRTVLLSIQALMGAPNPDDPLD 121
Query: 526 NDVAELWKVNESEAIXNAKEWTRRYAM 606
NDVA++WK NE +AI NA+EWT++YA+
Sbjct: 122 NDVAKIWKENEPQAIANAREWTKKYAV 148
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 132 bits (318), Expect = 6e-32
Identities = 64/144 (44%), Positives = 87/144 (60%)
Frame = +1
Query: 175 RRIIKETQRLMQEPVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMS 354
+RI +E L ++P SA P + ++ + GP DS DYP
Sbjct: 4 KRINRELADLGKDPPSSCSAGPVGDDLFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFK 63
Query: 355 APKVRFITKIYHPNIDRLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDV 534
PKV F T+IYHPNI+ G ICLDIL+D+WSPAL I VLLSI +LL+ PNPDDPL ++
Sbjct: 64 PPKVNFTTRIYHPNINSNGSICLDILRDQWSPALTISKVLLSICSLLTDPNPDDPLVPEI 123
Query: 535 AELWKVNESEAIXNAKEWTRRYAM 606
A ++K + S +A+EWTR+YA+
Sbjct: 124 AHVYKTDRSRYELSAREWTRKYAI 147
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 109 bits (261), Expect = 5e-25
Identities = 53/134 (39%), Positives = 75/134 (55%), Gaps = 1/134 (0%)
Frame = +1
Query: 166 ALPRRIIKETQRLMQEPVPGISAVP-SETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXD 342
++ +R+ E LM PGISA P S++N ++ +TGP D+ +
Sbjct: 28 SVTKRLRSELMSLMMSNTPGISAFPDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPAN 87
Query: 343 YPMSAPKVRFITKIYHPNIDRLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPL 522
YP S P + F + ++HPN+D G ICLDILKDKWS ++T+LLS+Q+LL PN PL
Sbjct: 88 YPYSPPTIIFTSPMWHPNVDMSGNICLDILKDKWSAVYNVQTILLSLQSLLGEPNNASPL 147
Query: 523 ANDVAELWKVNESE 564
AELW + E
Sbjct: 148 NAQAAELWSKDPIE 161
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 107 bits (257), Expect = 2e-24
Identities = 53/144 (36%), Positives = 76/144 (52%), Gaps = 1/144 (0%)
Frame = +1
Query: 175 RRIIKETQRLMQEPVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMS 354
RRI KE + Q+ GI + + + GPE + DYP
Sbjct: 7 RRIAKELADVQQDKQAGIQVWTINDDISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYPFR 66
Query: 355 APKVRFITKIYHPNID-RLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLAND 531
PK+ F TKIYHPN+ + G ICLDILKD+WSP +++ L+S+Q+LL P P +P
Sbjct: 67 PPKMNFDTKIYHPNVSSQTGAICLDILKDQWSPVYTMKSALISLQSLLCTPEPSNPQDAQ 126
Query: 532 VAELWKVNESEAIXNAKEWTRRYA 603
VA+++ N + + A+EWT YA
Sbjct: 127 VAQVYLQNYQQFVRTAREWTSSYA 150
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 102 bits (245), Expect = 4e-23
Identities = 43/137 (31%), Positives = 77/137 (56%)
Frame = +1
Query: 175 RRIIKETQRLMQEPVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMS 354
RR++++ +R+ Q+P G+SA P N ++ ++ GP D+ YP
Sbjct: 7 RRLMRDFKRMQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNK 66
Query: 355 APKVRFITKIYHPNIDRLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLANDV 534
P V+F++ ++HPN+ G +CLDIL+++WSP + +L SIQ+LL+ PN P +
Sbjct: 67 PPLVKFVSTMFHPNVYANGELCLDILQNRWSPTYDVAAILTSIQSLLNDPNNASPANAEA 126
Query: 535 AELWKVNESEAIXNAKE 585
A+L + N+ E + ++
Sbjct: 127 AQLHRENKKEYVRRVRK 143
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 97.5 bits (232), Expect = 2e-21
Identities = 49/144 (34%), Positives = 74/144 (51%), Gaps = 1/144 (0%)
Frame = +1
Query: 175 RRIIKETQRLMQEPVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMS 354
RR+ KE L + P+P I + N ++ GP DS DYP
Sbjct: 10 RRLTKEYSDLREHPIPDIRVNLVDDNLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQ 69
Query: 355 APKVRFITKIYHPNIDRLGRICLDILKDK-WSPALQIRTVLLSIQALLSAPNPDDPLAND 531
P + F T+IYHPN D G +CL ILK + + P++++R+VL I LL PNPDDPL
Sbjct: 70 PPTIEFTTRIYHPNFDSEGNVCLAILKQQVFKPSIKLRSVLEQILQLLREPNPDDPLVAS 129
Query: 532 VAELWKVNESEAIXNAKEWTRRYA 603
+AE ++ + A+++ ++A
Sbjct: 130 IAEQYRNDRPSFDKIARDYVEQFA 153
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 76.6 bits (180), Expect = 3e-15
Identities = 30/66 (45%), Positives = 45/66 (68%)
Frame = +1
Query: 340 DYPMSAPKVRFITKIYHPNIDRLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDP 519
+YP PKV+ + KIYHPNID G +CL+IL+ W+P L + ++L+ +Q L +PN +DP
Sbjct: 79 NYPHDPPKVKCLNKIYHPNIDIEGNVCLNILRQDWNPVLNLNSILVGLQFLFLSPNAEDP 138
Query: 520 LANDVA 537
L + A
Sbjct: 139 LNKEAA 144
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 75.4 bits (177), Expect = 7e-15
Identities = 43/134 (32%), Positives = 65/134 (48%), Gaps = 3/134 (2%)
Frame = +1
Query: 175 RRIIKETQRLMQEPVPGISAVPSETNARYFH--VIVTGPEDSXXXXXXXXXXXXXXXDYP 348
+R+ KE + G+ + T+ FH ++ GP ++ YP
Sbjct: 6 KRLHKEYASFQAGDISGLLLLKPVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYP 65
Query: 349 MSAPKVRFITKIYHPNIDRL-GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLA 525
+S P V F TKI HPNI G +C+DILK WSPA +++ L+I +LLS + PL
Sbjct: 66 ISPPSVYFQTKIVHPNISWTNGEVCMDILKTHWSPAWSLQSACLAIISLLSNYDASSPLN 125
Query: 526 NDVAELWKVNESEA 567
D A+L + + A
Sbjct: 126 VDAAKLLRTGDKTA 139
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 72.5 bits (170), Expect = 5e-14
Identities = 40/151 (26%), Positives = 72/151 (47%), Gaps = 14/151 (9%)
Frame = +1
Query: 187 KETQRLMQEPVPGISA-VPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPK 363
K+ + + + P G S + + + + V++ GPED+ DYP+ PK
Sbjct: 12 KQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPK 71
Query: 364 VRFITKIYHPNIDRLGRICLDILK-------------DKWSPALQIRTVLLSIQALLSAP 504
++F T+I+HPN+ G +C+ IL ++W P T+L+S+ ++LS+P
Sbjct: 72 MKFTTEIWHPNVHPNGEVCISILHPPGDDKYGYEDAGERWLPVHSPETILISVISMLSSP 131
Query: 505 NPDDPLANDVAELWKVNESEAIXNAKEWTRR 597
N + P D A+ ++ N E + RR
Sbjct: 132 NDESPANIDAAKEFRENPQEFKKRVRRLVRR 162
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 71.7 bits (168), Expect = 9e-14
Identities = 41/150 (27%), Positives = 70/150 (46%), Gaps = 2/150 (1%)
Frame = +1
Query: 160 MAALPRRIIKETQRLMQEPVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXX 339
M++ RRI + +L+ ++ V N + F+V GP ++
Sbjct: 1 MSSPRRRIETDVMKLLMSDYE-VTLV--NDNMQEFYVRFHGPSETPYSGGIWKVHVELPS 57
Query: 340 DYPMSAPKVRFITKIYHPNIDRL-GRICLDILKDKWSPALQIRTVL-LSIQALLSAPNPD 513
+YP +P + F+ +I+HPNID L G +CLD++ WSP + + + + LL PN
Sbjct: 58 EYPWKSPSIGFVNRIFHPNIDELSGSVCLDVINQTWSPMFDMINIFEVFLPQLLRYPNAS 117
Query: 514 DPLANDVAELWKVNESEAIXNAKEWTRRYA 603
DPL + A L + +++ RYA
Sbjct: 118 DPLNGEAAALLLREPNTYYAKVRDYIARYA 147
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 70.5 bits (165), Expect = 2e-13
Identities = 36/144 (25%), Positives = 70/144 (48%), Gaps = 14/144 (9%)
Frame = +1
Query: 175 RRIIKETQRLMQEPVPGISAVPSETNARY-FHVIVTGPEDSXXXXXXXXXXXXXXXDYPM 351
RR++KE + L + GI+A PS + + + ++ GP+ + DYP+
Sbjct: 8 RRLMKEYKELTENGPDGITAGPSNEDDFFTWDCLIQGPDGTPFEGGLYPATLKFPSDYPL 67
Query: 352 SAPKVRFITKIYHPNIDRLGRICLDIL-------------KDKWSPALQIRTVLLSIQAL 492
P ++F + +HPN+ + G +C+ IL ++WSP + +LLS+ ++
Sbjct: 68 GPPTLKFECEFFHPNVYKDGTVCISILHAPGDDPNMYESSSERWSPVQSVEKILLSVMSM 127
Query: 493 LSAPNPDDPLANDVAELWKVNESE 564
L+ PN + D ++W+ + E
Sbjct: 128 LAEPNDESGANIDACKMWREDREE 151
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 63.7 bits (148), Expect = 2e-11
Identities = 29/77 (37%), Positives = 44/77 (57%), Gaps = 2/77 (2%)
Frame = +1
Query: 340 DYPMSAPKVRFITKIYHPNIDRLGRICLDILKDK--WSPALQIRTVLLSIQALLSAPNPD 513
+YP PK RF ++HPN+ G +CL IL ++ W PA+ I+ +LL IQ LL PN
Sbjct: 67 EYPTRPPKCRFTPPLFHPNVYPSGTVCLSILNEEEGWKPAITIKQILLGIQDLLDDPNIA 126
Query: 514 DPLANDVAELWKVNESE 564
P + ++K ++ E
Sbjct: 127 SPAQTEAYTMFKKDKVE 143
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 54.4 bits (125), Expect = 1e-08
Identities = 30/112 (26%), Positives = 51/112 (45%), Gaps = 3/112 (2%)
Frame = +1
Query: 175 RRIIKETQRLMQEPVPGISAVPSETNARYFHVIVTGPEDSXXXXXXXXXXXXXXXDYPMS 354
+R++KE L + PV + A P+ N +H I+TGP D+ DYP
Sbjct: 8 KRLMKEYLALQKNPVELVDAKPATENILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFK 67
Query: 355 APKVRFITKIYHPNIDRLGRICL---DILKDKWSPALQIRTVLLSIQALLSA 501
P +R IT R+CL D W+P+ + T+L+ + + +++
Sbjct: 68 PPAIRMITP--SGRFQTNTRLCLSFSDFHPKSWNPSWMVSTILVGLVSFMTS 117
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 27.9 bits (59), Expect = 1.5
Identities = 19/79 (24%), Positives = 35/79 (44%), Gaps = 4/79 (5%)
Frame = +1
Query: 262 FHVIVTGPEDSXXXXXXXXXXXXXXXDYPMSAPKVRFITKIYHPNID-RLGRI---CLDI 429
++ + GP S +YP + P V F+++I P +D G++ +D
Sbjct: 41 WNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFVSRINLPGVDGETGKVNPHKIDC 100
Query: 430 LKDKWSPALQIRTVLLSIQ 486
L+ W + TVLL ++
Sbjct: 101 LR-HWKREYSMETVLLDLK 118
>SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr
3|||Manual
Length = 923
Score = 27.1 bits (57), Expect = 2.6
Identities = 9/26 (34%), Positives = 16/26 (61%)
Frame = +1
Query: 367 RFITKIYHPNIDRLGRICLDILKDKW 444
+FI ++HP+ RL R+C + + W
Sbjct: 219 KFILSVFHPDAARLYRLCSSLKRICW 244
>SPCC757.11c |||membrane transporter|Schizosaccharomyces pombe|chr
3|||Manual
Length = 471
Score = 25.4 bits (53), Expect = 7.9
Identities = 8/26 (30%), Positives = 15/26 (57%)
Frame = +1
Query: 352 SAPKVRFITKIYHPNIDRLGRICLDI 429
++P V +TK+ HP +G + + I
Sbjct: 393 NSPSVSLLTKVLHPKYHLIGNVAVQI 418
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,682,873
Number of Sequences: 5004
Number of extensions: 51080
Number of successful extensions: 123
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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