BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0671
(428 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 29 0.31
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 28 0.53
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 26 2.2
SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces... 25 3.8
SPCC553.02 |||glutamine-dependent NAD|Schizosaccharomyces pombe|... 24 8.7
SPBC409.09c |mis13|cnl1|kinetochore protein Mis13|Schizosaccharo... 24 8.7
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 24 8.7
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 29.1 bits (62), Expect = 0.31
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = +3
Query: 162 QKELEGIYNPIITNMYQ 212
QKELEG+ NPI+ +YQ
Sbjct: 594 QKELEGVANPIMAKIYQ 610
Score = 27.5 bits (58), Expect = 0.93
Identities = 12/24 (50%), Positives = 16/24 (66%), Gaps = 1/24 (4%)
Frame = +2
Query: 86 LDKC-NDXIKWLDSNQLADKEEYE 154
+DK + I+WLD N A K+EYE
Sbjct: 568 IDKAVKETIEWLDHNTTAAKDEYE 591
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 28.3 bits (60), Expect = 0.53
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +2
Query: 77 QTILDKCNDXIKWLDSNQLADKEEYE 154
+T+ + I+WLDSN A K+E+E
Sbjct: 566 ETVDKAVKETIEWLDSNTTAAKDEFE 591
Score = 26.6 bits (56), Expect = 1.6
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = +3
Query: 162 QKELEGIYNPIITNMYQ 212
QKELE + NPI+ +YQ
Sbjct: 594 QKELESVANPIMAKIYQ 610
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 26.2 bits (55), Expect = 2.2
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +1
Query: 220 EESPEVCRASRAEHPEPE 273
EE+P AS EHPEP+
Sbjct: 244 EEAPAAAAASENEHPEPK 261
>SPAC343.07 |mug28||RNA-binding protein Mug28|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 609
Score = 25.4 bits (53), Expect = 3.8
Identities = 10/31 (32%), Positives = 17/31 (54%)
Frame = +2
Query: 86 LDKCNDXIKWLDSNQLADKEEYEHXTERIGR 178
+D CN +K LD N + + ++ E + GR
Sbjct: 416 IDPCNLFVKNLDDNIVGNTQQLEELFSKFGR 446
>SPCC553.02 |||glutamine-dependent NAD|Schizosaccharomyces pombe|chr
3|||Manual
Length = 700
Score = 24.2 bits (50), Expect = 8.7
Identities = 9/21 (42%), Positives = 10/21 (47%)
Frame = -3
Query: 348 KCGVECWFNRXXRWWGQRLQP 286
KCG F + WG RL P
Sbjct: 610 KCGPYSMFTQLMHQWGDRLSP 630
>SPBC409.09c |mis13|cnl1|kinetochore protein
Mis13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 329
Score = 24.2 bits (50), Expect = 8.7
Identities = 12/40 (30%), Positives = 19/40 (47%)
Frame = +1
Query: 247 SRAEHPEPEVPPPGLEALAPPSXXSIKPTFHTTLKPTCNN 366
S+ + + + P P +A P+ +KP FHT L N
Sbjct: 228 SKQDLDKQDSPLPPDDAPELPNISKLKPKFHTLLDMLAEN 267
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 24.2 bits (50), Expect = 8.7
Identities = 8/16 (50%), Positives = 9/16 (56%)
Frame = +1
Query: 262 PEPEVPPPGLEALAPP 309
P P PPPG+ PP
Sbjct: 761 PPPPPPPPGVAGAGPP 776
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,245,452
Number of Sequences: 5004
Number of extensions: 19481
Number of successful extensions: 64
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 62
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 64
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 154448264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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