Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0664
(300 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g12450.1 68417.m01970 expressed protein 30 0.25
At1g27060.1 68414.m03299 regulator of chromosome condensation (R... 29 0.58
At3g61940.1 68416.m06956 zinc transporter, putative similar to z... 27 2.3
At2g07630.1 68415.m00881 hypothetical protein 27 2.3
At5g53440.1 68418.m06641 expressed protein 27 3.1
At2g12100.1 68415.m01300 Ulp1 protease family protein contains P... 27 3.1
At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains... 27 3.1
At1g45090.1 68414.m05169 Ulp1 protease family protein similar to... 27 3.1
At5g45350.1 68418.m05567 proline-rich family protein contains pr... 26 4.1
At4g09060.1 68417.m01493 expressed protein 26 4.1
At5g35750.1 68418.m04281 histidine kinase (AHK2) identical to hi... 26 5.4
At4g18160.1 68417.m02698 outward rectifying potassium channel, p... 26 5.4
At4g11610.1 68417.m01859 C2 domain-containing protein contains I... 26 5.4
At2g46610.2 68415.m05813 arginine/serine-rich splicing factor, p... 26 5.4
At2g46610.1 68415.m05814 arginine/serine-rich splicing factor, p... 26 5.4
At1g21580.1 68414.m02698 hydroxyproline-rich glycoprotein family... 26 5.4
At5g64200.2 68418.m08063 arginine/serine-rich splicing factor SC... 25 7.1
At5g64200.1 68418.m08062 arginine/serine-rich splicing factor SC... 25 7.1
At4g35480.1 68417.m05042 zinc finger (C3HC4-type RING finger) fa... 25 7.1
At4g21070.1 68417.m03047 BRCT domain-containing protein / zinc f... 25 7.1
At4g15545.1 68417.m02375 expressed protein 25 7.1
At1g21630.1 68414.m02708 calcium-binding EF hand family protein ... 25 7.1
At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to ... 25 9.4
At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to ... 25 9.4
At3g45060.1 68416.m04857 high-affinity nitrate transporter, puta... 25 9.4
At3g43590.1 68416.m04638 zinc knuckle (CCHC-type) family protein... 25 9.4
At3g13090.1 68416.m01639 ABC transporter, putative similar to MR... 25 9.4
At2g19270.1 68415.m02249 expressed protein 25 9.4
>At4g12450.1 68417.m01970 expressed protein
Length = 277
Score = 30.3 bits (65), Expect = 0.25
Identities = 17/57 (29%), Positives = 27/57 (47%)
Frame = -2
Query: 290 CRAVSRDIRTTTEARRPGTARNTRRPKPTELSVCCRERSETPRHRNKPSRPVWSRRS 120
CR RD+ T +PGT + +P P+ +C R E + + P+R S R+
Sbjct: 36 CRKNVRDVVNT----QPGTVKKNPKPDPSLRRLCSSRRPELDSNSHHPTRRSVSARA 88
>At1g27060.1 68414.m03299 regulator of chromosome condensation
(RCC1) family protein low similiarity to UVB-resistance
protein UVR8 [Arabidopsis thaliana] GI:5478530; contains
Pfam profile PF00415: Regulator of chromosome
condensation (RCC1)
Length = 386
Score = 29.1 bits (62), Expect = 0.58
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = +3
Query: 237 AWAPGLCGGPDVPAHGSAP 293
+W G CGGPDV A S P
Sbjct: 229 SWGRGFCGGPDVHAPQSLP 247
>At3g61940.1 68416.m06956 zinc transporter, putative similar to zinc
transporter ZAT [Arabidopsis thaliana]
gi|4206640|gb|AAD11757; similar to zinc transporter
ZnT-2 [Rattus norvegicus] gi|1256378|gb|AAB02775; member
of the cation diffusion facilitator (CDF) family, or
cation efflux (CE) family, PMID:11500563
Length = 334
Score = 27.1 bits (57), Expect = 2.3
Identities = 14/29 (48%), Positives = 16/29 (55%)
Frame = -1
Query: 273 GHQDHHRGPAPRHSEKHSASEADRAERLL 187
GH DH G HS H S +RAE+LL
Sbjct: 157 GH-DHGHGHDHGHSHDHGHSYGERAEQLL 184
>At2g07630.1 68415.m00881 hypothetical protein
Length = 458
Score = 27.1 bits (57), Expect = 2.3
Identities = 19/57 (33%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +2
Query: 65 QQLNCSSSISKYYNGNGVDSVETKQVEKVYSGDGASLSAPGSKRSA-LSASDAECFS 232
Q L C + +K Y + DS++ + YS + + + P SKRS+ S S A+ FS
Sbjct: 369 QYLVCVENENKRYQMH--DSIDQCSLMLTYSEEPSEATTPSSKRSSDTSISPADNFS 423
>At5g53440.1 68418.m06641 expressed protein
Length = 1181
Score = 26.6 bits (56), Expect = 3.1
Identities = 10/34 (29%), Positives = 19/34 (55%)
Frame = -1
Query: 297 RAVQSREQGHQDHHRGPAPRHSEKHSASEADRAE 196
R+++ E GH+++ R + E+H E +AE
Sbjct: 550 RSIEVEETGHRNNARDYSATEEERHLVDETSQAE 583
>At2g12100.1 68415.m01300 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At5g28270, At2g05450,
At1g45090, At2g16180, At2g06750
Length = 1224
Score = 26.6 bits (56), Expect = 3.1
Identities = 11/34 (32%), Positives = 17/34 (50%)
Frame = -1
Query: 255 RGPAPRHSEKHSASEADRAERLLPGALRDAPSPE 154
R P+P H +H + E+LLP + P+ E
Sbjct: 681 RVPSPSHQPEHGIPDGGNFEQLLPDPILSDPALE 714
>At2g03150.1 68415.m00268 ATP/GTP-binding protein family contains
ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
Length = 1340
Score = 26.6 bits (56), Expect = 3.1
Identities = 15/53 (28%), Positives = 23/53 (43%)
Frame = -2
Query: 254 EARRPGTARNTRRPKPTELSVCCRERSETPRHRNKPSRPVWSRRSPHHFHCSI 96
E +R + R P S +ER+ P+ ++ +R RR HH SI
Sbjct: 418 ERKRALEIKRDRTPTARATSKDTKERTPVPKSISRDARSSSLRRDAHHREASI 470
>At1g45090.1 68414.m05169 Ulp1 protease family protein similar to
At5g28270, At2g12100, At2g05450, At2g16180, At2g06750;
contains Pfam profile PF02902: Ulp1 protease family,
C-terminal catalytic domain
Length = 1210
Score = 26.6 bits (56), Expect = 3.1
Identities = 11/34 (32%), Positives = 17/34 (50%)
Frame = -1
Query: 255 RGPAPRHSEKHSASEADRAERLLPGALRDAPSPE 154
R P+P H +H + E+LLP + P+ E
Sbjct: 672 RVPSPSHQPEHGIPDGGNFEQLLPDPILSDPALE 705
>At5g45350.1 68418.m05567 proline-rich family protein contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 177
Score = 26.2 bits (55), Expect = 4.1
Identities = 10/18 (55%), Positives = 10/18 (55%)
Frame = +3
Query: 243 APGLCGGPDVPAHGSAPP 296
APG G P P HG PP
Sbjct: 73 APGYGGYPPAPGHGGYPP 90
>At4g09060.1 68417.m01493 expressed protein
Length = 341
Score = 26.2 bits (55), Expect = 4.1
Identities = 13/42 (30%), Positives = 21/42 (50%), Gaps = 2/42 (4%)
Frame = -2
Query: 248 RRPGTARNTRRPKPTELSVCCRERSE--TPRHRNKPSRPVWS 129
R +A++ +R P +S +R E +P KP PVW+
Sbjct: 290 RNSASAKSNKRKSPKTMSSPVEKRLEFSSPEISRKPLSPVWN 331
>At5g35750.1 68418.m04281 histidine kinase (AHK2) identical to
histidine kinase AHK2 [Arabidopsis thaliana]
gi|13537196|dbj|BAB40774
Length = 1176
Score = 25.8 bits (54), Expect = 5.4
Identities = 11/39 (28%), Positives = 21/39 (53%)
Frame = +2
Query: 71 LNCSSSISKYYNGNGVDSVETKQVEKVYSGDGASLSAPG 187
+ SS S+ Y G G+ +K++ ++ G+ +S PG
Sbjct: 816 MQADSSTSRTYGGTGIGLSISKRLVELMQGEMGFVSEPG 854
>At4g18160.1 68417.m02698 outward rectifying potassium channel,
putative (KCO6) similar to kco1 [Arabidopsis thaliana]
gi|2230761|emb|CAA69158; member of the 2 pore, 4
transmembrane (2P/4TM) K+ channel family, PMID:11500563
Length = 436
Score = 25.8 bits (54), Expect = 5.4
Identities = 12/30 (40%), Positives = 22/30 (73%), Gaps = 1/30 (3%)
Frame = +1
Query: 193 TLSSVGFGRRVFLAVPGRR-ASVVVLMSLL 279
++++VG+G R F +PGR A++ +L+S L
Sbjct: 310 SVTTVGYGDRAFKTLPGRLFAAIWLLVSTL 339
>At4g11610.1 68417.m01859 C2 domain-containing protein contains
INTERPRO:IPR000008 C2 domain
Length = 1011
Score = 25.8 bits (54), Expect = 5.4
Identities = 9/36 (25%), Positives = 17/36 (47%)
Frame = -1
Query: 294 AVQSREQGHQDHHRGPAPRHSEKHSASEADRAERLL 187
++ + + H +HH P+H SE R +L+
Sbjct: 193 SLAAEQDNHNEHHHHYVPKHQVDEMRSEPARPSKLV 228
>At2g46610.2 68415.m05813 arginine/serine-rich splicing factor,
putative similar to SP|P92964 Arginine/serine-rich
splicing factor RSP31 {Arabidopsis thaliana}
Length = 224
Score = 25.8 bits (54), Expect = 5.4
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -2
Query: 185 RERSETPRHRNKPSRPVWSRRSPHH 111
R RS +P +R +PS RRSP +
Sbjct: 154 RRRSPSPVYRRRPSPDYTRRRSPEY 178
>At2g46610.1 68415.m05814 arginine/serine-rich splicing factor,
putative similar to SP|P92964 Arginine/serine-rich
splicing factor RSP31 {Arabidopsis thaliana}
Length = 250
Score = 25.8 bits (54), Expect = 5.4
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -2
Query: 185 RERSETPRHRNKPSRPVWSRRSPHH 111
R RS +P +R +PS RRSP +
Sbjct: 180 RRRSPSPVYRRRPSPDYTRRRSPEY 204
>At1g21580.1 68414.m02698 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965
Length = 1696
Score = 25.8 bits (54), Expect = 5.4
Identities = 14/46 (30%), Positives = 17/46 (36%)
Frame = -2
Query: 248 RRPGTARNTRRPKPTELSVCCRERSETPRHRNKPSRPVWSRRSPHH 111
RR + R P+ R ETPR WSR+ HH
Sbjct: 300 RREASNELNRTPRKQVQKKSALLRLETPRSYKNSRENEWSRQHNHH 345
>At5g64200.2 68418.m08063 arginine/serine-rich splicing factor SC35
contains similarity to splicing factor; contains Pfam
profile PF00076: RNA recognition motif. (a.k.a. RRM,
RBD, or RNP domain)
Length = 303
Score = 25.4 bits (53), Expect = 7.1
Identities = 17/59 (28%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = -2
Query: 287 RAVSRDIRTTTEARRPGTARNTRRPKPTELSVCCR--ERSETPRHRNKPSRPVWSRRSP 117
R+ SR + + R P ++ + E+S R ERS +PR P P + SP
Sbjct: 206 RSHSRSLSASPARRSPRSSSPQKTSPAREVSPDKRSNERSPSPRRSLSPRSPALQKASP 264
>At5g64200.1 68418.m08062 arginine/serine-rich splicing factor SC35
contains similarity to splicing factor; contains Pfam
profile PF00076: RNA recognition motif. (a.k.a. RRM,
RBD, or RNP domain)
Length = 303
Score = 25.4 bits (53), Expect = 7.1
Identities = 17/59 (28%), Positives = 26/59 (44%), Gaps = 2/59 (3%)
Frame = -2
Query: 287 RAVSRDIRTTTEARRPGTARNTRRPKPTELSVCCR--ERSETPRHRNKPSRPVWSRRSP 117
R+ SR + + R P ++ + E+S R ERS +PR P P + SP
Sbjct: 206 RSHSRSLSASPARRSPRSSSPQKTSPAREVSPDKRSNERSPSPRRSLSPRSPALQKASP 264
>At4g35480.1 68417.m05042 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 200
Score = 25.4 bits (53), Expect = 7.1
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +3
Query: 195 AQLCRLRTPSVSRCAWAPGLCGGPDVPAHGSAPP 296
A +C +V+RCAW L G + A G APP
Sbjct: 36 ALVCVAGLAAVARCAWLRRLT-GVNPAAVGEAPP 68
>At4g21070.1 68417.m03047 BRCT domain-containing protein / zinc
finger (C3HC4-type RING finger) family protein (BRCA1)
contains Pfam profiles PF00533: BRCA1 C Terminus (BRCT)
domain, PF00097: Zinc finger, C3HC4 type (RING finger),
PF01535: PPR repeat; identical to cDNA BRCA1 GI:28372473
Length = 1276
Score = 25.4 bits (53), Expect = 7.1
Identities = 10/22 (45%), Positives = 13/22 (59%)
Frame = -1
Query: 276 QGHQDHHRGPAPRHSEKHSASE 211
+G QD GP+ H EK S +E
Sbjct: 764 KGDQDQAHGPSDTHPEKRSPTE 785
>At4g15545.1 68417.m02375 expressed protein
Length = 337
Score = 25.4 bits (53), Expect = 7.1
Identities = 15/43 (34%), Positives = 19/43 (44%)
Frame = -1
Query: 240 RHSEKHSASEADRAERLLPGALRDAPSPE*TFSTCLVSTESTP 112
RHS S ++ E DAP P + S LVS +TP
Sbjct: 168 RHSSIQSQQASEAIEPAATDNENDAPKPSLSASLPLVSQTTTP 210
>At1g21630.1 68414.m02708 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain; ESTs gb|T44428 and gb|AA395440 come from this
gene
Length = 1218
Score = 25.4 bits (53), Expect = 7.1
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = -3
Query: 298 PGGAEP*AGTSGPPQRP 248
PGG P AG G P RP
Sbjct: 540 PGGLRPPAGPKGKPPRP 556
>At5g62760.2 68418.m07879 nuclear protein ZAP-related similar to
nuclear protein ZAP, Mus musculus, EMBL:AB033168 this
cDNA provides a truncated ORF likely due to a skipped
exon. An alternative ORF is provided.
Length = 383
Score = 25.0 bits (52), Expect = 9.4
Identities = 13/40 (32%), Positives = 19/40 (47%)
Frame = +2
Query: 110 NGVDSVETKQVEKVYSGDGASLSAPGSKRSALSASDAECF 229
+ +D +VEKV D SLS+ SKR + C+
Sbjct: 311 HSMDDYFMTEVEKVEESDSTSLSSGRSKRPIVKTVMEYCY 350
>At5g62760.1 68418.m07878 nuclear protein ZAP-related similar to
nuclear protein ZAP, Mus musculus, EMBL:AB033168 this
cDNA provides a truncated ORF likely due to a skipped
exon. An alternative ORF is provided.
Length = 661
Score = 25.0 bits (52), Expect = 9.4
Identities = 13/40 (32%), Positives = 19/40 (47%)
Frame = +2
Query: 110 NGVDSVETKQVEKVYSGDGASLSAPGSKRSALSASDAECF 229
+ +D +VEKV D SLS+ SKR + C+
Sbjct: 311 HSMDDYFMTEVEKVEESDSTSLSSGRSKRPIVKTVMEYCY 350
>At3g45060.1 68416.m04857 high-affinity nitrate transporter,
putative similar to trans-membrane nitrate transporter
protein AtNRT2:1 [Arabidopsis thaliana] GI:3747058,
high-affinity nitrate transporter ACH1 [Arabidopsis
thaliana] GI:3608362
Length = 542
Score = 25.0 bits (52), Expect = 9.4
Identities = 19/57 (33%), Positives = 25/57 (43%), Gaps = 8/57 (14%)
Frame = -1
Query: 300 LRAVQSREQGHQDHHRGPAPRHSEK----HSAS----EADRAERLLPGALRDAPSPE 154
LR Q E+ ++H+ G EK H S E R+ER L D P+PE
Sbjct: 479 LRPSQDGEKSKEEHYYGAEWTEEEKSLGLHEGSIKFAENSRSERGRKAMLADIPTPE 535
>At3g43590.1 68416.m04638 zinc knuckle (CCHC-type) family protein
contains Pfam domain, PF00098: Zinc knuckle
Length = 551
Score = 25.0 bits (52), Expect = 9.4
Identities = 9/24 (37%), Positives = 12/24 (50%)
Frame = -2
Query: 176 SETPRHRNKPSRPVWSRRSPHHFH 105
S++ RH + PS W HH H
Sbjct: 492 SQSSRHHSGPSPSRWQPSHQHHHH 515
>At3g13090.1 68416.m01639 ABC transporter, putative similar to
MRP-like ABC transporter [Arabidopsis thaliana]
GI:2316016; contains Pfam profile: PF00005 ABC
transporter
Length = 1466
Score = 25.0 bits (52), Expect = 9.4
Identities = 13/44 (29%), Positives = 20/44 (45%)
Frame = +2
Query: 77 CSSSISKYYNGNGVDSVETKQVEKVYSGDGASLSAPGSKRSALS 208
C S + K G +D ++ + S D ++AP SK LS
Sbjct: 173 CCSCLWKKGEGERIDLLKEPLLSSAESSDNEEVTAPFSKAGILS 216
>At2g19270.1 68415.m02249 expressed protein
Length = 359
Score = 25.0 bits (52), Expect = 9.4
Identities = 19/45 (42%), Positives = 21/45 (46%)
Frame = +2
Query: 119 DSVETKQVEKVYSGDGASLSAPGSKRSALSASDAECFSLCLGAGP 253
D E K +K + AS S S RS LSA A S LGA P
Sbjct: 111 DEEEEKARKKRKQMESASASHDSSVRSFLSAMPAPKSSQTLGALP 155
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,982,885
Number of Sequences: 28952
Number of extensions: 132154
Number of successful extensions: 502
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 488
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 502
length of database: 12,070,560
effective HSP length: 70
effective length of database: 10,043,920
effective search space used: 291273680
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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