Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0657
(700 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 288 3e-80
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 285 4e-79
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 178 5e-47
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 145 4e-37
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 31 0.011
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.7
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 22 4.9
AY268031-1|AAP23056.1| 810|Apis mellifera dorsal protein splice... 22 4.9
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 288 bits (707), Expect = 3e-80
Identities = 136/141 (96%), Positives = 137/141 (97%)
Frame = +1
Query: 19 RXKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 198
+ K HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK
Sbjct: 3 KEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 62
Query: 199 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVXAGTGE 378
LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTS ADCAVLIV AGTGE
Sbjct: 63 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 122
Query: 379 FEAGISKNGQTREHALLAFTL 441
FEAGISKNGQTREHALLAFTL
Sbjct: 123 FEAGISKNGQTREHALLAFTL 143
Score = 74.5 bits (175), Expect = 9e-16
Identities = 33/58 (56%), Positives = 39/58 (67%)
Frame = +3
Query: 483 PLNHHTXSPDLRKSXRKYPHTXRKXGYNPSAVAFVPISGWHGXNMLEPSTKMPWFXGW 656
P T +++K Y +K GYNP+AVAFVPISGWHG NMLE S+KMPWF GW
Sbjct: 160 PPYSETRFEEIKKEVSSY---IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGW 214
Score = 72.1 bits (169), Expect = 5e-15
Identities = 35/47 (74%), Positives = 35/47 (74%)
Frame = +2
Query: 443 GVKQLIVGVNKMDSTEPPYXEPRFEEIXKEVSSYXKKDXLQPICCRF 583
GVKQLIVGVNKMDSTEPPY E RFEEI KEVSSY KK P F
Sbjct: 144 GVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAF 190
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 285 bits (698), Expect = 4e-79
Identities = 134/141 (95%), Positives = 136/141 (96%)
Frame = +1
Query: 19 RXKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 198
+ K HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK
Sbjct: 3 KEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 62
Query: 199 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVXAGTGE 378
LKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTS ADCAVLIV AG GE
Sbjct: 63 LKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGE 122
Query: 379 FEAGISKNGQTREHALLAFTL 441
FEAGISKNGQTREHALLAFTL
Sbjct: 123 FEAGISKNGQTREHALLAFTL 143
Score = 68.5 bits (160), Expect = 6e-14
Identities = 26/36 (72%), Positives = 30/36 (83%)
Frame = +3
Query: 549 RKXGYNPSAVAFVPISGWHGXNMLEPSTKMPWFXGW 656
+K GYN ++VAFVPISGWHG NMLEPS K PW+ GW
Sbjct: 179 KKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGW 214
Score = 66.9 bits (156), Expect = 2e-13
Identities = 31/37 (83%), Positives = 32/37 (86%)
Frame = +2
Query: 443 GVKQLIVGVNKMDSTEPPYXEPRFEEIXKEVSSYXKK 553
GVKQLIVGVNKMD T+PPY E RFEEI KEVSSY KK
Sbjct: 144 GVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 178 bits (433), Expect = 5e-47
Identities = 84/86 (97%), Positives = 84/86 (97%)
Frame = +1
Query: 184 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVX 363
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTS ADCAVLIV
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 364 AGTGEFEAGISKNGQTREHALLAFTL 441
AGTGEFEAGISKNGQTREHALLAFTL
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTL 86
Score = 74.5 bits (175), Expect = 9e-16
Identities = 33/58 (56%), Positives = 39/58 (67%)
Frame = +3
Query: 483 PLNHHTXSPDLRKSXRKYPHTXRKXGYNPSAVAFVPISGWHGXNMLEPSTKMPWFXGW 656
P T +++K Y +K GYNP+AVAFVPISGWHG NMLE S+KMPWF GW
Sbjct: 103 PPYSETRFEEIKKEVSSY---IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGW 157
Score = 72.1 bits (169), Expect = 5e-15
Identities = 35/47 (74%), Positives = 35/47 (74%)
Frame = +2
Query: 443 GVKQLIVGVNKMDSTEPPYXEPRFEEIXKEVSSYXKKDXLQPICCRF 583
GVKQLIVGVNKMDSTEPPY E RFEEI KEVSSY KK P F
Sbjct: 87 GVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAF 133
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 145 bits (351), Expect = 4e-37
Identities = 68/70 (97%), Positives = 68/70 (97%)
Frame = +1
Query: 232 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLIVXAGTGEFEAGISKNGQT 411
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTS ADCAVLIV AGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 412 REHALLAFTL 441
REHALLAFTL
Sbjct: 61 REHALLAFTL 70
Score = 74.5 bits (175), Expect = 9e-16
Identities = 33/58 (56%), Positives = 39/58 (67%)
Frame = +3
Query: 483 PLNHHTXSPDLRKSXRKYPHTXRKXGYNPSAVAFVPISGWHGXNMLEPSTKMPWFXGW 656
P T +++K Y +K GYNP+AVAFVPISGWHG NMLE S+KMPWF GW
Sbjct: 87 PPYSETRFEEIKKEVSSY---IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGW 141
Score = 72.1 bits (169), Expect = 5e-15
Identities = 35/47 (74%), Positives = 35/47 (74%)
Frame = +2
Query: 443 GVKQLIVGVNKMDSTEPPYXEPRFEEIXKEVSSYXKKDXLQPICCRF 583
GVKQLIVGVNKMDSTEPPY E RFEEI KEVSSY KK P F
Sbjct: 71 GVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAF 117
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 31.1 bits (67), Expect = 0.011
Identities = 15/35 (42%), Positives = 17/35 (48%)
Frame = +1
Query: 271 VTIIDAPGHRDFIKNMITGTSHADCAVLIVXAGTG 375
VT +D PGH FI G D VL+V A G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
Score = 25.4 bits (53), Expect = 0.52
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 25 KTHINIVVIGHVDSGKST 78
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.2
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 34 INIVVIGHVDSGKST 78
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1
Query: 256 TSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLI 357
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.2 bits (45), Expect = 4.9
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +1
Query: 256 TSKYYVTIIDAPGHRDFIKNMITGTSHADCAVLI 357
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>AY268031-1|AAP23056.1| 810|Apis mellifera dorsal protein splice
variant B protein.
Length = 810
Score = 22.2 bits (45), Expect = 4.9
Identities = 8/36 (22%), Positives = 20/36 (55%)
Frame = +2
Query: 467 VNKMDSTEPPYXEPRFEEIXKEVSSYXKKDXLQPIC 574
+N+M + P Y + +++ E+S+ K + ++ C
Sbjct: 450 LNRMYKSYPNYIDKETKDMNLEISTRPKSNTVENAC 485
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 190,180
Number of Sequences: 438
Number of extensions: 3822
Number of successful extensions: 24
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 24
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 21439440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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