Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0654
(700 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 462 e-132
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 447 e-128
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 352 2e-99
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 319 2e-89
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 38 1e-04
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.8
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.7
AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 21 8.5
AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 8.5
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 462 bits (1139), Expect = e-132
Identities = 217/229 (94%), Positives = 220/229 (96%)
Frame = +3
Query: 12 RXKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 191
+ K HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK
Sbjct: 3 KEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 62
Query: 192 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 371
LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE
Sbjct: 63 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 122
Query: 372 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIG 551
FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIG
Sbjct: 123 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIG 182
Query: 552 YNPAAVAFVPISGWHGDNMLEPSTKMPWFXGWQVERXESKADGKCLIEA 698
YNPAAVAFVPISGWHGDNMLE S+KMPWF GW VER E K +GKCLIEA
Sbjct: 183 YNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEA 231
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 447 bits (1101), Expect = e-128
Identities = 209/229 (91%), Positives = 216/229 (94%)
Frame = +3
Query: 12 RXKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 191
+ K HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK
Sbjct: 3 KEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 62
Query: 192 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 371
LKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GE
Sbjct: 63 LKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGE 122
Query: 372 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIG 551
FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYIKKIG
Sbjct: 123 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIG 182
Query: 552 YNPAAVAFVPISGWHGDNMLEPSTKMPWFXGWQVERXESKADGKCLIEA 698
YN A+VAFVPISGWHGDNMLEPS K PW+ GW+VER + ADGK LIEA
Sbjct: 183 YNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEA 231
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 352 bits (865), Expect = 2e-99
Identities = 165/174 (94%), Positives = 167/174 (95%)
Frame = +3
Query: 177 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 356
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 357 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 536
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 537 IKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFXGWQVERXESKADGKCLIEA 698
IKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWF GW VER E K +GKCLIEA
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEA 174
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 319 bits (783), Expect = 2e-89
Identities = 149/158 (94%), Positives = 151/158 (95%)
Frame = +3
Query: 225 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 404
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 405 REHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPI 584
REHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 585 SGWHGDNMLEPSTKMPWFXGWQVERXESKADGKCLIEA 698
SGWHGDNMLE S+KMPWF GW VER E K +GKCLIEA
Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEA 158
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 37.5 bits (83), Expect = 1e-04
Identities = 36/117 (30%), Positives = 52/117 (44%)
Frame = +3
Query: 264 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 443
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 444 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE 614
+IV +NK+D + ++E K + I+++G + V IS G N+ E
Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELG---GEIQCVKISALKGINLRE 298
Score = 25.4 bits (53), Expect = 0.52
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +3
Query: 18 KTHINIVVIGHVDSGKST 71
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.2
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +3
Query: 27 INIVVIGHVDSGKST 71
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 2.8
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 249 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 350
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.7
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +3
Query: 249 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 350
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic
acetylcholine Apisa7-2 subunit protein.
Length = 461
Score = 21.4 bits (43), Expect = 8.5
Identities = 8/24 (33%), Positives = 13/24 (54%)
Frame = -3
Query: 626 FG*RLQHVVSVPSRNGHESDSSWV 555
FG L H++ V +N + + WV
Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58
>AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase
protein.
Length = 580
Score = 21.4 bits (43), Expect = 8.5
Identities = 7/20 (35%), Positives = 13/20 (65%)
Frame = +1
Query: 469 WIPLNHHTVSPDLRKSRRKY 528
W+P+N + S +L +R+Y
Sbjct: 443 WLPVNENYKSLNLAAQKREY 462
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 198,236
Number of Sequences: 438
Number of extensions: 4177
Number of successful extensions: 17
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 21439440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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