Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0654
(700 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 357 4e-99
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 357 4e-99
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 357 4e-99
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 357 4e-99
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 200 9e-52
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 196 9e-51
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 103 9e-23
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 99 1e-21
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 56 2e-08
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 56 2e-08
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 52 3e-07
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 52 4e-07
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 52 5e-07
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 47 1e-05
At5g13650.2 68418.m01585 elongation factor family protein contai... 42 3e-04
At5g13650.1 68418.m01584 elongation factor family protein contai... 42 3e-04
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 42 5e-04
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 42 5e-04
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 40 0.001
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 40 0.001
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 39 0.003
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 37 0.015
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 37 0.015
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 36 0.034
At3g01360.1 68416.m00057 expressed protein contains Pfam profile... 33 0.24
At5g46180.1 68418.m05680 ornithine aminotransferase, putative / ... 31 0.97
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.3
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 30 1.3
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.7
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.7
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 29 2.2
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 5.2
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M... 28 6.8
At1g26130.1 68414.m03190 haloacid dehalogenase-like hydrolase fa... 28 6.8
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 27 9.0
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 27 9.0
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 357 bits (878), Expect = 4e-99
Identities = 166/211 (78%), Positives = 185/211 (87%)
Frame = +3
Query: 12 RXKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 191
+ K HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDK
Sbjct: 3 KEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDK 62
Query: 192 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 371
LKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG
Sbjct: 63 LKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGG 122
Query: 372 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIG 551
FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK+G
Sbjct: 123 FEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVG 182
Query: 552 YNPAAVAFVPISGWHGDNMLEPSTKMPWFXG 644
YNP + FVPISG+ GDNM+E ST + W+ G
Sbjct: 183 YNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 357 bits (878), Expect = 4e-99
Identities = 166/211 (78%), Positives = 185/211 (87%)
Frame = +3
Query: 12 RXKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 191
+ K HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDK
Sbjct: 3 KEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDK 62
Query: 192 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 371
LKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG
Sbjct: 63 LKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGG 122
Query: 372 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIG 551
FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK+G
Sbjct: 123 FEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVG 182
Query: 552 YNPAAVAFVPISGWHGDNMLEPSTKMPWFXG 644
YNP + FVPISG+ GDNM+E ST + W+ G
Sbjct: 183 YNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 357 bits (878), Expect = 4e-99
Identities = 166/211 (78%), Positives = 185/211 (87%)
Frame = +3
Query: 12 RXKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 191
+ K HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDK
Sbjct: 3 KEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDK 62
Query: 192 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 371
LKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG
Sbjct: 63 LKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGG 122
Query: 372 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIG 551
FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK+G
Sbjct: 123 FEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVG 182
Query: 552 YNPAAVAFVPISGWHGDNMLEPSTKMPWFXG 644
YNP + FVPISG+ GDNM+E ST + W+ G
Sbjct: 183 YNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 357 bits (878), Expect = 4e-99
Identities = 166/211 (78%), Positives = 185/211 (87%)
Frame = +3
Query: 12 RXKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 191
+ K HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDK
Sbjct: 3 KEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDK 62
Query: 192 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 371
LKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG
Sbjct: 63 LKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGG 122
Query: 372 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIG 551
FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK+G
Sbjct: 123 FEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVG 182
Query: 552 YNPAAVAFVPISGWHGDNMLEPSTKMPWFXG 644
YNP + FVPISG+ GDNM+E ST + W+ G
Sbjct: 183 YNPDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 200 bits (487), Expect = 9e-52
Identities = 97/215 (45%), Positives = 142/215 (66%), Gaps = 3/215 (1%)
Frame = +3
Query: 9 ARXKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLD 188
A K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 96 ANKKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMD 155
Query: 189 KLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 368
+ ER +G T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A G
Sbjct: 156 TNEEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKG 215
Query: 369 EFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKI 548
EFE G + GQTREH LA TLGV +LIV VNKMD +S+ R++EI++++ ++K
Sbjct: 216 EFETGYERGGQTREHVQLAKTLGVSKLIVVVNKMDDPTVNWSKERYDEIEQKMVPFLKAS 275
Query: 549 GYNPAA-VAFVPISGWHGDNMLEPSTK--MPWFXG 644
GYN V F+PISG G NM + + PW+ G
Sbjct: 276 GYNTKKDVVFLPISGLMGKNMDQRMGQEICPWWSG 310
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 196 bits (479), Expect = 9e-51
Identities = 91/212 (42%), Positives = 141/212 (66%), Gaps = 4/212 (1%)
Frame = +3
Query: 21 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 200
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 201 ERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA 380
ERERGIT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEA
Sbjct: 298 ERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEA 357
Query: 381 GISK-NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYN 557
G GQTREHA + GV+Q+IV +NKMD YS+ RF+ IK+ V S+++ +
Sbjct: 358 GFDNLKGQTREHARVLRGFGVEQVIVAINKMDIV--GYSKERFDLIKQHVGSFLQSCRFK 415
Query: 558 PAAVAFVPISGWHGDNML-EPSTK--MPWFXG 644
+++ ++P+S N++ PS W+ G
Sbjct: 416 DSSLTWIPLSAMENQNLVAAPSDNRLSSWYQG 447
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 103 bits (248), Expect = 9e-23
Identities = 64/179 (35%), Positives = 93/179 (51%), Gaps = 1/179 (0%)
Frame = +3
Query: 3 KWARXKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV 182
K+ R K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE--------------- 116
Query: 183 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 362
+D ER RGITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+
Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176
Query: 363 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE-EIKKEVSSY 536
G QT+EH LLA +GV ++V +NK D + E E+++ +SSY
Sbjct: 177 DGPMP-------QTKEHILLAKQVGVPDMVVFLNKEDQVDDAELLELVELEVRELLSSY 228
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 99 bits (238), Expect = 1e-21
Identities = 67/200 (33%), Positives = 98/200 (49%)
Frame = +3
Query: 6 WARXKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 185
+ R K H+N+ IGHVD GK+T T + K E GK +
Sbjct: 61 FTRNKPHVNVGTIGHVDHGKTTLTAAIT---------------KVLAEEGKAKAIAFDEI 105
Query: 186 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 365
DK E++RGITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+
Sbjct: 106 DKAPEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPD 165
Query: 366 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 545
G QT+EH LLA +GV L+ +NK+D + P E +E+ S+ K
Sbjct: 166 GPMP-------QTKEHILLARQVGVPSLVCFLNKVDVVDDPELLELVEMELRELLSFYKF 218
Query: 546 IGYNPAAVAFVPISGWHGDN 605
G + + +S G N
Sbjct: 219 PGDDIPIIRGSALSALQGTN 238
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 56.4 bits (130), Expect = 2e-08
Identities = 41/128 (32%), Positives = 61/128 (47%)
Frame = +3
Query: 30 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 209
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 210 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 389
+GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 390 KNGQTREH 413
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 56.4 bits (130), Expect = 2e-08
Identities = 41/128 (32%), Positives = 61/128 (47%)
Frame = +3
Query: 30 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 209
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 210 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 389
+GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 390 KNGQTREH 413
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 52.4 bits (120), Expect = 3e-07
Identities = 52/174 (29%), Positives = 81/174 (46%), Gaps = 4/174 (2%)
Frame = +3
Query: 30 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 209
N +I H+D GKST L+ G + R + K F LD + ERE
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131
Query: 210 RGITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFE 377
RGITI + + +E + + + +ID PGH DF + + + A+L+V A G E
Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG-VE 190
Query: 378 AGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 539
A QT + LA + ++I +NK+D P +EP E++ +E+ I
Sbjct: 191 A------QTLANVYLALENNL-EIIPVLNKIDL---PGAEP--EKVLREIEEVI 232
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 52.0 bits (119), Expect = 4e-07
Identities = 36/113 (31%), Positives = 53/113 (46%)
Frame = +3
Query: 30 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 209
NI ++ HVD GK+T HLI GG + GK F +D L E+
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG--------GVLHPRLAGKLRF-----MDYLDEEQR 57
Query: 210 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 368
R IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 58 RAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 51.6 bits (118), Expect = 5e-07
Identities = 36/117 (30%), Positives = 56/117 (47%)
Frame = +3
Query: 18 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 197
K + NI ++ H+D+GK+TTT ++Y G K E+ +G+ W ++
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTGRNYK---------IGEVHEGTATMDW----ME 140
Query: 198 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 368
E+ERGITI A K+ + IID PGH DF + D A+ + + G
Sbjct: 141 QEQERGITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 47.2 bits (107), Expect = 1e-05
Identities = 54/177 (30%), Positives = 76/177 (42%), Gaps = 9/177 (5%)
Frame = +3
Query: 30 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 209
N +I H+D GKST L+ G I K G G +Y LDKL +RE
Sbjct: 68 NFSIIAHIDHGKSTLADRLMELTGTIKK-------------GHGQPQY---LDKL--QRE 109
Query: 210 RGITIDIALWKF---------ETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 362
RGIT+ E S Y + +ID PGH DF + S A+L+V A
Sbjct: 110 RGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVVDAA 169
Query: 363 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSS 533
G QT + LAF + ++ +NK+D P ++P E +K ++ S
Sbjct: 170 QG-------VQAQTVANFYLAFEANL-TIVPVINKIDQ---PTADP--ERVKAQLKS 213
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 42.3 bits (95), Expect = 3e-04
Identities = 46/159 (28%), Positives = 66/159 (41%)
Frame = +3
Query: 30 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 209
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 85 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 129
Query: 210 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 389
RGITI V IID PGH DF + + D +L+V + G
Sbjct: 130 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 185
Query: 390 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 506
QTR A G ++V VNK+D P + P F
Sbjct: 186 ---QTRFVLKKALEFG-HAVVVVVNKIDR---PSARPEF 217
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 42.3 bits (95), Expect = 3e-04
Identities = 46/159 (28%), Positives = 66/159 (41%)
Frame = +3
Query: 30 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 209
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 84 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 128
Query: 210 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 389
RGITI V IID PGH DF + + D +L+V + G
Sbjct: 129 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 184
Query: 390 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 506
QTR A G ++V VNK+D P + P F
Sbjct: 185 ---QTRFVLKKALEFG-HAVVVVVNKIDR---PSARPEF 216
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 41.5 bits (93), Expect = 5e-04
Identities = 25/95 (26%), Positives = 46/95 (48%)
Frame = +3
Query: 249 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 428
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 429 TLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSS 533
+ +K +I+ NK+D + + E I+K +++
Sbjct: 178 MMRLKHIIILQNKIDLINEKAATEQHEAIQKFITN 212
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 41.5 bits (93), Expect = 5e-04
Identities = 25/95 (26%), Positives = 46/95 (48%)
Frame = +3
Query: 249 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 428
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 429 TLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSS 533
+ +K +I+ NK+D + + E I+K +++
Sbjct: 178 MMRLKHIIILQNKIDLINEKAATEQHEAIQKFITN 212
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 40.3 bits (90), Expect = 0.001
Identities = 24/91 (26%), Positives = 46/91 (50%)
Frame = +3
Query: 261 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 440
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 441 KQLIVGVNKMDSTEPPYSEPRFEEIKKEVSS 533
K +I+ NK+D + + + E+I++ +++
Sbjct: 174 KDIIIIQNKIDLIQENEAIKQHEDIQRFITN 204
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 40.3 bits (90), Expect = 0.001
Identities = 28/103 (27%), Positives = 49/103 (47%), Gaps = 4/103 (3%)
Frame = +3
Query: 243 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 410
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 411 HALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 539
H + +K +I+ NK+D + + + E I+K + + +
Sbjct: 166 HLAAVEIMQLKHIIILQNKIDLIQENVAINQHEAIQKFIMNTV 208
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 39.1 bits (87), Expect = 0.003
Identities = 30/112 (26%), Positives = 51/112 (45%), Gaps = 4/112 (3%)
Frame = +3
Query: 30 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRT---IEKFEKEAQEMGKG-SFKYAWVLDKLK 197
N+ VI HVD GKST T L+ G I + + + A E +G + K + +
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGISLYYE 80
Query: 198 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 353
E ++ + ++Y + +ID+PGH DF + D A+++V
Sbjct: 81 MTDE---SLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.7 bits (81), Expect = 0.015
Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%)
Frame = +3
Query: 30 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 209
N+ ++GH+ GK+ L+ + + K EK KY D E+E
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187
Query: 210 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 368
R I+I + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.7 bits (81), Expect = 0.015
Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%)
Frame = +3
Query: 30 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 209
N+ ++GH+ GK+ L+ + + K EK KY D E+E
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187
Query: 210 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 368
R I+I + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 35.5 bits (78), Expect = 0.034
Identities = 33/105 (31%), Positives = 50/105 (47%), Gaps = 3/105 (2%)
Frame = +3
Query: 264 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 443
+T +D PGH F + G + D VL+VAA G QT E A + V
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDGVMP-------QTLEAIAHARSANV- 321
Query: 444 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSS---YIKKIGYNPAAV 569
++V +NK D P + P E++K +++S ++ IG N AV
Sbjct: 322 PVVVAINKCDK---PGANP--EKVKYQLTSEGIELEDIGGNVQAV 361
>At3g01360.1 68416.m00057 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 319
Score = 32.7 bits (71), Expect = 0.24
Identities = 27/96 (28%), Positives = 44/96 (45%), Gaps = 1/96 (1%)
Frame = +3
Query: 273 IDAPGHRDFIKNMITGTSQADCAV-LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 449
I GH D+ + T Q +C + L+V TG F +KNG R+ LG +
Sbjct: 226 IKCKGHGDYHRAKAIATLQFNCHLALMVVVATGLFSVIANKNGYLRQDHSKYRPLGAELE 285
Query: 450 IVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYN 557
+ +DS E +E+++E S+ K++G N
Sbjct: 286 NLSTFTLDSDEE-------DEVREE-SNVAKEVGLN 313
>At5g46180.1 68418.m05680 ornithine aminotransferase, putative /
ornithine--oxo-acid aminotransferase, putative similar
to SP|Q92413 Ornithine aminotransferase (EC 2.6.1.13)
(Ornithine--oxo-acid aminotransferase) [Aspergillus
nidulans] {Emericella nidulans}; contains Pfam profile
PF00202: aminotransferase, class III
Length = 475
Score = 30.7 bits (66), Expect = 0.97
Identities = 17/54 (31%), Positives = 28/54 (51%)
Frame = +3
Query: 429 TLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISG 590
T GV++ G+ + +S PP S R E++ E S++ Y+P V F +G
Sbjct: 18 TAGVRRSYGGLPQSNSKSPPSSSQRLMELESEFSAH----NYHPVPVVFSRANG 67
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 30.3 bits (65), Expect = 1.3
Identities = 21/68 (30%), Positives = 34/68 (50%)
Frame = +3
Query: 270 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 449
+ID PGH F G+S D A+L+V + + G+ QT E +L + +
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIKHGLEP--QTIE-SLNLLRMRNTEF 756
Query: 450 IVGVNKMD 473
I+ +NK+D
Sbjct: 757 IIALNKVD 764
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 30.3 bits (65), Expect = 1.3
Identities = 22/70 (31%), Positives = 33/70 (47%)
Frame = +3
Query: 264 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 443
+ +ID PGH F G++ D A+L+V + G+ QT E L VK
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV-----DIMRGLEP--QTIESLNLLRRRNVK 610
Query: 444 QLIVGVNKMD 473
I+ +NK+D
Sbjct: 611 -FIIALNKVD 619
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 270 IIDAPGHRDFIKNMITGTSQADCAVLIV 353
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.7
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 270 IIDAPGHRDFIKNMITGTSQADCAVLIV 353
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 29.5 bits (63), Expect = 2.2
Identities = 26/99 (26%), Positives = 38/99 (38%), Gaps = 4/99 (4%)
Frame = +3
Query: 189 KLKAERERGITIDIALWKFET----SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 356
K+ A GIT I +K +D PGH F G D A+++VA
Sbjct: 525 KVAASEAGGITQGIGAYKVSVPVDGKLQSCVFLDTPGHEAFGAMRARGARVTDIAIIVVA 584
Query: 357 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 473
A G QT E A+ +++ +NK+D
Sbjct: 585 ADDG-------IRPQTNE-AIAHAKAAAVPIVIAINKID 615
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 5.2
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +3
Query: 492 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP 581
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
Monovalent cation:proton antiporter family 2 (CPA2
family) member, PMID:11500563; related to
glutathione-regulated potassium-efflux system protein
[Escherichia coli] GP|606284|gb|AAA58147
Length = 568
Score = 27.9 bits (59), Expect = 6.8
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +3
Query: 498 PRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNM 608
PRF ++ ++SS ++ Y AAVAF +S W D +
Sbjct: 354 PRFLKLMIQLSSQTNEL-YQLAAVAFCLLSAWCSDKL 389
>At1g26130.1 68414.m03190 haloacid dehalogenase-like hydrolase
family protein similar to Potential
phospholipid-transporting ATPase (EC 3.6.3.1) from Homo
sapiens [SP|Q9Y2Q0, SP|O43520], Mus musculus [SP|P98200,
SP|P70704], {Bos taurus} SP|Q29449; contains InterPro
accession IPR005834: Haloacid dehalogenase-like
hydrolase
Length = 1184
Score = 27.9 bits (59), Expect = 6.8
Identities = 15/53 (28%), Positives = 24/53 (45%)
Frame = +3
Query: 375 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSS 533
E+G +TR+H G++ LI+ ++D E R E K VS+
Sbjct: 629 ESGRKYEKETRDHVNEYADAGLRTLILAYRELDENEYEVFTERISEAKNSVSA 681
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 27.5 bits (58), Expect = 9.0
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +3
Query: 474 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 587
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 27.5 bits (58), Expect = 9.0
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +3
Query: 474 STEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPIS 587
+TE + P+ E K E+S++I IG+ V F P S
Sbjct: 63 ATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCS 100
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,337,843
Number of Sequences: 28952
Number of extensions: 326764
Number of successful extensions: 1052
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 1004
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1036
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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