Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0611
(600 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme Rad31|Schiz... 103 2e-23
SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ... 77 3e-15
SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ... 64 2e-11
SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pomb... 55 7e-09
SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz... 54 2e-08
SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ... 44 1e-05
SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ... 36 0.003
SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 30 0.22
SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc... 27 2.1
SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 27 2.8
SPAC4A8.14 |prs1||ribose-phosphate pyrophosphokinase Prs1|Schizo... 26 4.8
SPAC23C11.01 |||ER membrane protein, ICE2 family|Schizosaccharom... 25 6.4
>SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme
Rad31|Schizosaccharomyces pombe|chr 1|||Manual
Length = 307
Score = 103 bits (248), Expect = 2e-23
Identities = 53/150 (35%), Positives = 87/150 (58%)
Frame = +2
Query: 92 VGNNEVELSEAEAEQYDRQIRLWGLDSQKXLRAAKVLIIGLSGLGAEIAKNVILTGVKSV 271
+GN+ + E YDRQIRLWG ++Q+ L+ ++VL+I S L EIAKN++L+G+ +
Sbjct: 1 MGNHNINAEEIAL--YDRQIRLWGFNAQQALKQSRVLLITASPLANEIAKNLVLSGIGKL 58
Query: 272 CLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLERXRGLNPMVDVTSHTKGVXXLPDSFF 451
C+LD+ + + D+ QF IG RA ++ LNP+V++ + T + + +
Sbjct: 59 CVLDSMTVYEKDVEEQFFIEASDIGQLRANVFKKKLHELNPLVEIDTDTSLISEIDEGKI 118
Query: 452 TEFDVVCATGLKQDQFXRINNACRDSNKKF 541
++F +V AT L ++F RIN R N F
Sbjct: 119 SKFSMVIATQLDYEEFCRINELTRICNASF 148
>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1012
Score = 76.6 bits (180), Expect = 3e-15
Identities = 50/142 (35%), Positives = 70/142 (49%)
Frame = +2
Query: 137 YDRQIRLWGLDSQKXLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLYS 316
Y RQ+ + G ++ K + + VLIIG GLG EIAKNV L GVKSV L D + + DL S
Sbjct: 20 YSRQLYVLGHEAMKQMSQSNVLIIGCKGLGVEIAKNVCLAGVKSVTLYDPQPTRIEDLSS 79
Query: 317 QFLCPPDKIGVNRAEGSLERXRGLNPMVDVTSHTKGVXXLPDSFFTEFDVVCATGLKQDQ 496
Q+ D IGV RA+ ++ + LN V V+ V L + F V T +
Sbjct: 80 QYFLTEDDIGVPRAKVTVSKLAELNQYVPVSV----VDELSTEYLKNFKCVVVTETSLTK 135
Query: 497 FXRINNACRDSNKKFICGRRLG 562
IN+ ++ +I G
Sbjct: 136 QLEINDFTHKNHIAYIAADSRG 157
Score = 44.4 bits (100), Expect = 1e-05
Identities = 27/113 (23%), Positives = 57/113 (50%), Gaps = 7/113 (6%)
Frame = +2
Query: 134 QYDRQIRLWGLDSQKXLRAAKVLIIGLSGLGAEIAKNVILTGVKS-----VCLLDNEKLK 298
+YD QI ++G + Q+ + + ++G +G E+ KN + GV + + + D + ++
Sbjct: 409 RYDGQIAVFGSEFQEKIASLSTFLVGAGAIGCEMLKNWAMMGVATGESGHISVTDMDSIE 468
Query: 299 QIDLYSQFLCPPDKIGVNRAEGSLERXRGLNPMV--DVTSHTKGVXXLPDSFF 451
+ +L QFL P +G ++E + +NP + +TS+ + V + F
Sbjct: 469 KSNLNRQFLFRPRDVGKLKSECASTAVSIMNPSLTGKITSYQERVGPESEGIF 521
>SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 500
Score = 63.7 bits (148), Expect = 2e-11
Identities = 38/139 (27%), Positives = 69/139 (49%), Gaps = 3/139 (2%)
Frame = +2
Query: 116 SEAEAEQYDRQIRLWGLDSQKXLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKL 295
+ A+ ++YDRQ+RLW + Q + + V ++ + +G E KN+IL G+ S ++D+ +
Sbjct: 3 TSAKMQKYDRQVRLWKAEGQNAIEKSHVCLLYANTVGCEALKNLILPGIGSFAVVDDTSV 62
Query: 296 KQIDLYSQFLCPPDKIGVNRAEGSLERXRGLNPMVDVTSHTKGVXXLPD---SFFTEFDV 466
F D+ G +RA + + LNP V++ L D +F++F V
Sbjct: 63 DFSMDGMNFFIQYDQEGKSRARCTASLLQQLNPNVEMEYLEMSPEALIDKNIEYFSKFSV 122
Query: 467 VCATGLKQDQFXRINNACR 523
V ++ LK+ R+ R
Sbjct: 123 VLSSNLKEKPLFRLEEYLR 141
>SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 401
Score = 55.2 bits (127), Expect = 7e-09
Identities = 31/108 (28%), Positives = 60/108 (55%), Gaps = 2/108 (1%)
Frame = +2
Query: 98 NNEVELSEAEAEQYDRQIRLW--GLDSQKXLRAAKVLIIGLSGLGAEIAKNVILTGVKSV 271
+N +ELS E +Y RQ+ L GL Q L+ + VL+IG GLG + ++ G+ ++
Sbjct: 11 SNGLELSLDEYSRYGRQMLLSEIGLPGQLSLKRSSVLVIGAGGLGCPAMQYLVAAGIGTL 70
Query: 272 CLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLERXRGLNPMVDVTSH 415
++D + + + +L+ Q + K G+++A + + LNP V + ++
Sbjct: 71 GIMDGDVVDKSNLHRQIIHSTSKQGMHKAISAKQFLEDLNPNVIINTY 118
>SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme
Fub2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 628
Score = 53.6 bits (123), Expect = 2e-08
Identities = 30/121 (24%), Positives = 62/121 (51%), Gaps = 2/121 (1%)
Frame = +2
Query: 164 LDSQKXLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKI 343
+++ + ++AKVL++G G+G E+ KN++++GVK V ++D + + +L QFL +
Sbjct: 17 VEALRNFKSAKVLLVGAGGIGCELLKNLLMSGVKEVHIIDLDTIDLSNLNRQFLFRKKHV 76
Query: 344 GVNRAEGSLERXRGLNPMVDVTSHTKGV--XXLPDSFFTEFDVVCATGLKQDQFXRINNA 517
+A + + NP V + ++ + ++F +FD+V D +N
Sbjct: 77 KQPKAIVAAKTASSFNPNVKLEAYHANIKEDRFNVAWFRQFDLVFNALDNLDARRHVNKQ 136
Query: 518 C 520
C
Sbjct: 137 C 137
>SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 444
Score = 44.4 bits (100), Expect = 1e-05
Identities = 27/101 (26%), Positives = 48/101 (47%)
Frame = +2
Query: 167 DSQKXLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKIG 346
++ K ++K+LIIG GLG EI K++ L+G + + ++D + + +L QFL I
Sbjct: 37 ETLKSAFSSKILIIGAGGLGCEILKDLALSGFRDLSVIDMDTIDITNLNRQFLFNESNID 96
Query: 347 VNRAEGSLERXRGLNPMVDVTSHTKGVXXLPDSFFTEFDVV 469
+A + P VT + F+ EF ++
Sbjct: 97 EPKANVAASMIMKRIPSTVVTPFYGKIQDKTIEFYKEFKLI 137
>SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 485
Score = 36.3 bits (80), Expect = 0.003
Identities = 21/105 (20%), Positives = 48/105 (45%)
Frame = +2
Query: 131 EQYDRQIRLWGLDSQKXLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDL 310
EQ R +G D + LR + V+++G G+G+ + + +GV+ + ++D +++ L
Sbjct: 107 EQLARNYAFFGEDGMERLRNSFVIVVGCGGVGSWVINMLARSGVQKIRIVDFDQVSLSSL 166
Query: 311 YSQFLCPPDKIGVNRAEGSLERXRGLNPMVDVTSHTKGVXXLPDS 445
+ +G + + + P ++V + + PDS
Sbjct: 167 NRHSIATLQDVGTPKTLAIKKAIKKFAPWIEVDA--RNALFNPDS 209
>SPBC6B1.05c |||ubiquitin-like conjugating
enzyme|Schizosaccharomyces pombe|chr 2|||Manual
Length = 649
Score = 30.3 bits (65), Expect = 0.22
Identities = 28/113 (24%), Positives = 52/113 (46%), Gaps = 5/113 (4%)
Frame = +2
Query: 113 LSEAEAEQYDRQIRLWGLDSQKXL---RAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLD 283
LSE+ A + + W L Q L + +K L++G LG +A+N++ GV+ V +D
Sbjct: 308 LSES-ASTLNLSLMRWRLVPQLDLDRIQNSKCLLLGAGTLGCGVARNLLSWGVRHVTFVD 366
Query: 284 NEKLKQIDLYSQ--FLCPPDKIGVNRAEGSLERXRGLNPMVDVTSHTKGVXXL 436
+ + Q F K + +AE + +R + + P + T + + L
Sbjct: 367 YSTVSYSNPVRQSLFTFEDCKRKLPKAECAAQRLKEIYPNMFSTGYNISIPML 419
>SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1679
Score = 27.1 bits (57), Expect = 2.1
Identities = 20/54 (37%), Positives = 25/54 (46%), Gaps = 6/54 (11%)
Frame = -1
Query: 360 SARFTPILSGGHRNWEYRSIC---FSFSLSN---KHTLFTPVRITFFAISAPSP 217
SA IL+GGHRN +S C F L N H FT I +++P P
Sbjct: 806 SAEAMSILAGGHRNVFVKSTCPIFFQKQLDNYKADHYCFTLDFILTDCVNSPKP 859
>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
Cct6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 26.6 bits (56), Expect = 2.8
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +2
Query: 257 GVKSVCLLDNEKLKQIDLY 313
G SVCLL E LKQ +LY
Sbjct: 89 GTTSVCLLVGELLKQAELY 107
>SPAC4A8.14 |prs1||ribose-phosphate pyrophosphokinase
Prs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 25.8 bits (54), Expect = 4.8
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = +2
Query: 221 LGAEIAKNVILTGVKSVCLLDNEKLKQIDLYSQFLCPPD 337
LG I I+T SVC + ++++LYSQ CP D
Sbjct: 240 LGHIIDDEEIITTPASVC--SEDYAQEVNLYSQGGCPSD 276
>SPAC23C11.01 |||ER membrane protein, ICE2
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 25.4 bits (53), Expect = 6.4
Identities = 8/18 (44%), Positives = 13/18 (72%)
Frame = -2
Query: 107 LHYFQPFFAMLLFIISIN 54
LH+F+P +L II++N
Sbjct: 116 LHFFEPLLVFILLIIALN 133
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,349,112
Number of Sequences: 5004
Number of extensions: 44193
Number of successful extensions: 121
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 118
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 121
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 262236260
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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