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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0610
         (750 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative simi...    28   5.8  

>At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative similar
           to SP|P39940 Ubiquitin--protein ligase RSP5 (EC 6.3.2.-)
           {Saccharomyces cerevisiae}; contains Pfam profiles
           PF00240: Ubiquitin family, PF00632: HECT-domain
           (ubiquitin-transferase)
          Length = 873

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 21/95 (22%), Positives = 42/95 (44%), Gaps = 3/95 (3%)
 Frame = +2

Query: 206 LSNIIILYEEIKSTGNWNTEECKEYIKPLDKSFEILYGFPLDQILYINIFDKKDIFNVCI 385
           L+ + I  E+IK T       CK+ ++   + F+   G  L  +L      K+D   +C 
Sbjct: 629 LAGLKISLEDIKDTDRIMYNSCKQILEMDPEFFDSNAGLGLTFVLETEELGKRDTIELCP 688

Query: 386 KILHEKITVDSLKKYPSL---XEVYSSLVEKVKEY 481
               + +   + K+Y  L       + ++E+VK++
Sbjct: 689 DGKLKAVNSKNRKQYVDLLIERRFATPILEQVKQF 723


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,833,260
Number of Sequences: 28952
Number of extensions: 219852
Number of successful extensions: 429
Number of sequences better than 10.0: 1
Number of HSP's better than 10.0 without gapping: 428
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 429
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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