BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0596 (600 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb... 29 0.52 SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit Bgs3|Schizo... 27 2.1 SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pomb... 27 2.8 SPCC550.15c |||ribosome biogenesis protein |Schizosaccharomyces ... 27 2.8 SPAC977.16c |dak2||dihydroxyacetone kinase Dak2 |Schizosaccharom... 25 6.4 SPAC1D4.03c |aut12||autophagy associated protein Aut12|Schizosac... 25 6.4 SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl... 25 8.5 >SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 1919 Score = 29.1 bits (62), Expect = 0.52 Identities = 12/26 (46%), Positives = 17/26 (65%) Frame = -3 Query: 202 RAFQLIFLCYNAFIDVGNQSPRGVLV 125 +A + + LC+NAF+DV N P V V Sbjct: 1645 KASEFVILCFNAFLDVSNIFPAIVRV 1670 >SPAC19B12.03 |bgs3||1,3-beta-glucan synthase subunit Bgs3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1826 Score = 27.1 bits (57), Expect = 2.1 Identities = 10/22 (45%), Positives = 14/22 (63%) Frame = -2 Query: 530 VSGFCPYENSXNRFSVRPRMSG 465 ++GFCP+EN R R+SG Sbjct: 1028 INGFCPFENGRRLPKYRIRLSG 1049 >SPAC1006.04c |mcp3|mug7|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 952 Score = 26.6 bits (56), Expect = 2.8 Identities = 26/102 (25%), Positives = 44/102 (43%), Gaps = 9/102 (8%) Frame = +2 Query: 2 EYLVRECERTRTQTRVESLKILL--EELKA----RAGPFAAVGKLTADKHATRRLIANVD 163 EYL+ E + VES K+L EE+K + F V LT ++ ++ + N Sbjct: 448 EYLMEELNELKNNLEVESSKVLRLDEEMKCLKDEQLSQFDTVFSLTDERDGLQKDLKNTK 507 Query: 164 KSI---ITKKDELKRALSSLNVTDRKIANVRECLTAVFGCLQ 280 ++ I + LK + +T K+ + E L+ LQ Sbjct: 508 GNLDDEIGRSAFLKSQIRDQELTIEKLHDSLETLSQTNNSLQ 549 >SPCC550.15c |||ribosome biogenesis protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 463 Score = 26.6 bits (56), Expect = 2.8 Identities = 25/97 (25%), Positives = 42/97 (43%) Frame = +2 Query: 305 DRYKAHISLPQESIEQLQKFYDEKRSSRKMKLQLSFDLXASTSDSFNDRAENGTPTFADE 484 D Y S P +++Q + +E SS + + D+ + +S D E G ADE Sbjct: 299 DFYDFTTSYPDYAVKQDETVVEEDGSSGEGDWE---DVSDDSDNSSLDSLEMGRVPIADE 355 Query: 485 LKIYLXSFHMDKNRKLVLENGEKLWIFETVXXVGASL 595 +++L S + +R L + L T GAS+ Sbjct: 356 YELHLPSGNRVGHRSLSRYFRQNLHSSSTAVGDGASI 392 >SPAC977.16c |dak2||dihydroxyacetone kinase Dak2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 591 Score = 25.4 bits (53), Expect = 6.4 Identities = 16/63 (25%), Positives = 30/63 (47%) Frame = +2 Query: 38 QTRVESLKILLEELKARAGPFAAVGKLTADKHATRRLIANVDKSIITKKDELKRALSSLN 217 +T +++++ +E L G AA T TR++ A V ++ K+EL + S Sbjct: 510 RTLIDAIQPFVETLNEGKGLDAAAKAATEGSEQTRKMDAVVGRASYVAKEELHKLDSEGG 569 Query: 218 VTD 226 + D Sbjct: 570 LPD 572 >SPAC1D4.03c |aut12||autophagy associated protein Aut12|Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 25.4 bits (53), Expect = 6.4 Identities = 15/50 (30%), Positives = 25/50 (50%), Gaps = 4/50 (8%) Frame = +2 Query: 431 SDSFNDRAENGTP----TFADELKIYLXSFHMDKNRKLVLENGEKLWIFE 568 + SFND E+ P T + IY+ S+ + K+ L++ + E FE Sbjct: 315 TQSFNDSMEHWVPVCFPTLNPDAYIYIYSYFLCKDTVLIMGSSESGVFFE 364 >SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonuclease Cce1|Schizosaccharomyces pombe|chr 1|||Manual Length = 258 Score = 25.0 bits (52), Expect = 8.5 Identities = 18/67 (26%), Positives = 32/67 (47%), Gaps = 2/67 (2%) Frame = +2 Query: 365 YDEKRSSRKMKLQLSFDLXASTSDSFNDRAE--NGTPTFADELKIYLXSFHMDKNRKLVL 538 Y EKR+S + K+Q F L S +++ A N +F+ + + +K++ Sbjct: 147 YAEKRNSIEQKIQYPFLLSLSPKSTYSYWASVLNTKASFSKKKSRVQMVKELIDGQKILF 206 Query: 539 ENGEKLW 559 EN E L+ Sbjct: 207 ENEEALY 213 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,040,817 Number of Sequences: 5004 Number of extensions: 35204 Number of successful extensions: 142 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 262236260 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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