BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0595 (750 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g16730.1 68418.m01959 expressed protein weak similarity to mi... 32 0.47 At3g10370.1 68416.m01243 glycerol-3-phosphate dehydrogenase, put... 30 1.9 At5g36170.2 68418.m04359 peptide chain release factor, putative ... 29 2.5 At5g23100.1 68418.m02702 expressed protein contains Pfam profile... 29 2.5 At4g17300.1 68417.m02598 asparaginyl-tRNA synthetase, chloroplas... 29 2.5 At5g36170.3 68418.m04360 peptide chain release factor, putative ... 29 4.4 At5g36170.1 68418.m04358 peptide chain release factor, putative ... 29 4.4 At3g13290.1 68416.m01673 transducin family protein / WD-40 repea... 29 4.4 At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative... 28 5.8 At4g29250.1 68417.m04183 transferase family protein low similari... 28 5.8 At2g18090.1 68415.m02103 PHD finger family protein / SWIB comple... 28 5.8 At2g46270.1 68415.m05753 G-box binding factor 3 (GBF3) identical... 28 7.6 >At5g16730.1 68418.m01959 expressed protein weak similarity to microtubule binding protein D-CLIP-190 [Drosophila melanogaster] GI:2773363, SMC2-like condensin [Arabidopsis thaliana] GI:14279543 Length = 853 Score = 31.9 bits (69), Expect = 0.47 Identities = 15/30 (50%), Positives = 19/30 (63%) Frame = +3 Query: 18 SRELNTPNSRYSIDLCTPNSRLSKEINSPK 107 S TP+SR S+D +PNS+ S E SPK Sbjct: 44 SPSTTTPHSRLSLDRSSPNSKSSVERRSPK 73 >At3g10370.1 68416.m01243 glycerol-3-phosphate dehydrogenase, putative similar to glycerol-3-phosphate dehydrogenase GB:BAA08926 from Mus musculus [SP|Q64521], Homo sapiens [GI:1020315], Rattus norvegicus [SP|P35571]; contains Pfam profile PF01266 FAD dependent oxidoreductase Length = 629 Score = 29.9 bits (64), Expect = 1.9 Identities = 8/15 (53%), Positives = 11/15 (73%) Frame = -1 Query: 240 CFAPCFLWYELLYRW 196 C PCF W+E++Y W Sbjct: 164 CMTPCFDWFEVIYFW 178 >At5g36170.2 68418.m04359 peptide chain release factor, putative similar to SP|P28367 Peptide chain release factor 2 (RF-2) {Bacillus subtilis}; contains Pfam profiles PF00472: Peptidyl-tRNA hydrolase domain, PF03462: PCRF domain Length = 455 Score = 29.5 bits (63), Expect = 2.5 Identities = 18/56 (32%), Positives = 26/56 (46%), Gaps = 5/56 (8%) Frame = +3 Query: 15 LSRELNTPNSRYSIDLCTPNSRLSKEINSP-----KSRLSLDLNNGQERYITQEYF 167 L R N P R S+ L PN S NSP +S LS+ N ++ Q+++ Sbjct: 26 LLRPTNLPLLRLSLPLSLPNFSSSSRFNSPIFAAQESNLSVSNENETSEWLMQDFY 81 >At5g23100.1 68418.m02702 expressed protein contains Pfam profile PF04759: Protein of unknown function, DUF617 Length = 277 Score = 29.5 bits (63), Expect = 2.5 Identities = 17/52 (32%), Positives = 31/52 (59%) Frame = +3 Query: 15 LSRELNTPNSRYSIDLCTPNSRLSKEINSPKSRLSLDLNNGQERYITQEYFG 170 + ++ N+P + + I+L TP S L KE+ S R++L+ + G+E +E G Sbjct: 106 IQKDPNSPPA-FLIELATPISGLVKEMASGLVRIALECDKGKEEEEGEEKNG 156 >At4g17300.1 68417.m02598 asparaginyl-tRNA synthetase, chloroplast, mitochondrial / asparagine-tRNA ligase / AsnRS (SYNO) nearly identical to SP|O48593 Length = 567 Score = 29.5 bits (63), Expect = 2.5 Identities = 19/52 (36%), Positives = 29/52 (55%), Gaps = 2/52 (3%) Frame = +3 Query: 78 RLSKEINSPKSRLSLDLNNGQERYITQEYFGHSPK-SRKMPND-KAIHTKGN 227 + +K+ + P + LDL + ERYIT+E FG P R P + KA + + N Sbjct: 422 KANKKFDFPV-KWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMREN 472 >At5g36170.3 68418.m04360 peptide chain release factor, putative similar to SP|P28367 Peptide chain release factor 2 (RF-2) {Bacillus subtilis}; contains Pfam profiles PF00472: Peptidyl-tRNA hydrolase domain, PF03462: PCRF domain Length = 391 Score = 28.7 bits (61), Expect = 4.4 Identities = 18/57 (31%), Positives = 26/57 (45%), Gaps = 6/57 (10%) Frame = +3 Query: 15 LSRELNTPNSRYSIDLCTPNSRLSKEINSP------KSRLSLDLNNGQERYITQEYF 167 L R N P R S+ L PN S NSP +S LS+ N ++ Q+++ Sbjct: 26 LLRPTNLPLLRLSLPLSLPNFSSSSRFNSPIVFAAQESNLSVSNENETSEWLMQDFY 82 >At5g36170.1 68418.m04358 peptide chain release factor, putative similar to SP|P28367 Peptide chain release factor 2 (RF-2) {Bacillus subtilis}; contains Pfam profiles PF00472: Peptidyl-tRNA hydrolase domain, PF03462: PCRF domain Length = 456 Score = 28.7 bits (61), Expect = 4.4 Identities = 18/57 (31%), Positives = 26/57 (45%), Gaps = 6/57 (10%) Frame = +3 Query: 15 LSRELNTPNSRYSIDLCTPNSRLSKEINSP------KSRLSLDLNNGQERYITQEYF 167 L R N P R S+ L PN S NSP +S LS+ N ++ Q+++ Sbjct: 26 LLRPTNLPLLRLSLPLSLPNFSSSSRFNSPIVFAAQESNLSVSNENETSEWLMQDFY 82 >At3g13290.1 68416.m01673 transducin family protein / WD-40 repeat family protein contains 2 WD-40 repeats (PF00400); autoantigen locus HUMAUTANT (GI:533202) [Homo sapiens] and autoantigen locus HSU17474 (GI:596134) [Homo sapiens] Length = 1322 Score = 28.7 bits (61), Expect = 4.4 Identities = 12/40 (30%), Positives = 20/40 (50%) Frame = +3 Query: 168 GHSPKSRKMPNDKAIHTKGNMVQNKLSPQNRFTDFKKYSS 287 G PK R++ + A++ +Q ++ PQ T KY S Sbjct: 139 GKMPKGRRLVGEHAVYDVDVRLQGEIQPQLEVTPITKYGS 178 >At5g17000.1 68418.m01991 NADP-dependent oxidoreductase, putative strong similarity to probable NADP-dependent oxidoreductase (zeta-crystallin homolog) P1 [SP|Q39172][gi:886428] and P2 [SP|Q39173][gi:886430], Arabidopsis thaliana Length = 345 Score = 28.3 bits (60), Expect = 5.8 Identities = 13/48 (27%), Positives = 25/48 (52%), Gaps = 2/48 (4%) Frame = +3 Query: 192 MPNDKAIHTKGNMVQNKLSPQNR--FTDFKKYSSTFDNIQSLIREGKV 329 + N + +H N++ ++ Q F ++KYS D + I+EGK+ Sbjct: 262 LENQEGVHNLSNIIYKRIRIQGFAVFDFYEKYSKFLDFVLPHIKEGKI 309 >At4g29250.1 68417.m04183 transferase family protein low similarity to CER2 Arabidopsis thaliana GI:1213594, anthocyanin 5-aromatic acyltransferase Gentiana triflora GI:4185599; contains Pfam profile PF02458 transferase family Length = 460 Score = 28.3 bits (60), Expect = 5.8 Identities = 20/86 (23%), Positives = 34/86 (39%), Gaps = 1/86 (1%) Frame = +3 Query: 114 LSLDLNNGQERYITQEYFGHSPKSRKMPNDKAIHTKGNMVQNKLSPQNRFTDFKKYSSTF 293 +SL L+ + + Q YFG+ K+P K + TK ++ + + T Y + Sbjct: 294 MSLCLDVRKLLRLDQSYFGNCMVYHKVPYSKPVKTKDKLLFHAVQEIESITKRLDYDTVM 353 Query: 294 DNIQSL-IREGKVEEAPPTECNETVN 368 D I+ L G + C N Sbjct: 354 DLIEWLSSNNGAISNGSDLVCTNLEN 379 >At2g18090.1 68415.m02103 PHD finger family protein / SWIB complex BAF60b domain-containing protein / GYF domain-containing protein contains Pfam profiles PF02201: BAF60b domain of the SWIB complex, PF02213: GYF domain, PF00628: PHD-finger Length = 824 Score = 28.3 bits (60), Expect = 5.8 Identities = 20/59 (33%), Positives = 29/59 (49%), Gaps = 2/59 (3%) Frame = +3 Query: 249 PQNRFTDFKK-YSSTFD-NIQSLIREGKVEEAPPTECNETVNELMTVPPNMVRVVSLPS 419 P N+ DF +S TF N S + + A P EC+E+V++L+ M LPS Sbjct: 709 PDNQEIDFLDLFSPTFKFNFASTTTDWQPIVAGPDECDESVSDLLAEVEAMESQKRLPS 767 >At2g46270.1 68415.m05753 G-box binding factor 3 (GBF3) identical to G-box binding factor 3 (GBF3) SP:P42776 from [Arabidopsis thaliana]; contains Pfam profile: PF00170 bZIP transcription factor Length = 382 Score = 27.9 bits (59), Expect = 7.6 Identities = 16/69 (23%), Positives = 31/69 (44%) Frame = +3 Query: 540 KIENNISAILSQXRDQNHFNPYLQKDWNIXXDEYWDEVPNDIIENNYRYSSRERQKRHDI 719 K+ AILS N NP++ + + E W + ++ + S+RE +R + Sbjct: 220 KLIQGSGAILSPGVSANS-NPFMSQSLAMVPPETWLQNERELKRERRKQSNRESARRSRL 278 Query: 720 QKSSSXNEI 746 +K + E+ Sbjct: 279 RKQAETEEL 287 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,548,042 Number of Sequences: 28952 Number of extensions: 325745 Number of successful extensions: 914 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 884 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 914 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1663169840 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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