BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0575 (758 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 35 0.051 At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 34 0.12 At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 34 0.12 At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 33 0.21 At2g27100.1 68415.m03256 C2H2 zinc-finger protein SERRATE (SE) i... 31 0.83 At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 30 1.5 At5g55670.1 68418.m06941 RNA recognition motif (RRM)-containing ... 29 2.5 At4g14830.1 68417.m02280 expressed protein 29 3.4 At2g28680.1 68415.m03486 cupin family protein similar to legumin... 29 3.4 At5g18190.1 68418.m02135 protein kinase family protein contains ... 28 5.9 At4g36850.1 68417.m05225 PQ-loop repeat family protein / transme... 28 5.9 At3g49540.1 68416.m05414 expressed protein 28 5.9 At1g76770.1 68414.m08934 heat shock protein-related contains sim... 28 7.7 >At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein (HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853) (GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res. 17 (19), 7995 (1989)) Length = 157 Score = 35.1 bits (77), Expect = 0.051 Identities = 18/50 (36%), Positives = 31/50 (62%) Frame = +1 Query: 388 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAXKGERKVPIA 537 S +F RR+ LPE A E +++ + +GVL++T P KVP+ + + I+ Sbjct: 109 SGKFTRRFRLPENAKMEEIKASM-ENGVLSVTVP-KVPEKKPEVKSIDIS 156 >At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HSP18.1-CI) identical to 18.2 kDa class I heat shock protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana]; contains Pfam profile: PF00011 Hsp20/alpha crystallin family Length = 161 Score = 33.9 bits (74), Expect = 0.12 Identities = 18/50 (36%), Positives = 30/50 (60%) Frame = +1 Query: 388 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAXKGERKVPIA 537 S +F+RR+ LPE A E V++ + +GVLT+ P K P+ + + I+ Sbjct: 111 SGKFMRRFRLPENAKMEEVKATM-ENGVLTVVVP-KAPEKKPQVKSIDIS 158 >At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HSP17.4-CI) identical to 17.4 kDa class I heat shock protein SP:P19036 from [Arabidopsis thaliana] Length = 156 Score = 33.9 bits (74), Expect = 0.12 Identities = 19/50 (38%), Positives = 32/50 (64%) Frame = +1 Query: 388 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAXKGERKVPIA 537 S +F+RR+ LPE A E V++ + +GVL++T P KV ++ + V I+ Sbjct: 108 SGKFMRRFRLPENAKVEEVKASM-ENGVLSVTVP-KVQESKPEVKSVDIS 155 >At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein (HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha crystallin family; identified in Scharf, K-D., et al, Cell Stress & Chaperones (2001) 6: 225-237. Length = 153 Score = 33.1 bits (72), Expect = 0.21 Identities = 15/35 (42%), Positives = 23/35 (65%) Frame = +1 Query: 388 SRQFVRRYALPEGAAPETVESRLSSDGVLTITAPR 492 S QF RR+ LPE + V++ + +GVLT+T P+ Sbjct: 105 SGQFTRRFRLPENVKMDQVKAAM-ENGVLTVTVPK 138 >At2g27100.1 68415.m03256 C2H2 zinc-finger protein SERRATE (SE) identical to C2H2 zinc-finger protein SERRATE GI:14486602 from [Arabidopsis thaliana] Length = 720 Score = 31.1 bits (67), Expect = 0.83 Identities = 15/32 (46%), Positives = 16/32 (50%) Frame = +3 Query: 495 GTRRRQGREKGAHRTDRSRSQGD*GPERGNPG 590 G R R GR G R DRS Q + GNPG Sbjct: 582 GRRERDGRANGNDRNDRSEDQQRGDNDGGNPG 613 >At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock protein (HSP26.5-P) contains Pfam profile: PF00011 Hsp20/alpha crystallin family: identified in Scharf, K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237. Length = 134 Score = 30.3 bits (65), Expect = 1.5 Identities = 18/79 (22%), Positives = 33/79 (41%) Frame = +1 Query: 262 DKFQVNLDVQHFSPEEISVKTADGYIVVXXXXXXXXXXXXYISRQFVRRYALPEGAAPET 441 D ++D+ EEI V+ D ++ + F R++ LPE Sbjct: 35 DSHTFSVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIG 94 Query: 442 VESRLSSDGVLTITAPRKV 498 + + DGVLT+ P+++ Sbjct: 95 ISAGYE-DGVLTVIVPKRI 112 >At5g55670.1 68418.m06941 RNA recognition motif (RRM)-containing protein Length = 710 Score = 29.5 bits (63), Expect = 2.5 Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 5/39 (12%) Frame = +3 Query: 462 RRGSHHHRAEEGTR-----RRQGREKGAHRTDRSRSQGD 563 R HHH+ E +R R + RE+ HR +R R GD Sbjct: 619 REREHHHKDRERSREHVRDRERERERDRHREERERYGGD 657 >At4g14830.1 68417.m02280 expressed protein Length = 152 Score = 29.1 bits (62), Expect = 3.4 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = +1 Query: 400 VRRYALPEGAAPETVESRLSSDGVLTITAPR 492 V R+ LPE PE V DG L +T P+ Sbjct: 109 VWRFRLPESTRPELVTVDCDGDGELIVTVPK 139 >At2g28680.1 68415.m03486 cupin family protein similar to legumin (11S-globulin) from Ginkgo biloba [GI:949869], 11S globulin from Avena sativa [GI:472867]; contains a 11-S plant seed storage protein signature (PS00305) Length = 356 Score = 29.1 bits (62), Expect = 3.4 Identities = 21/64 (32%), Positives = 35/64 (54%), Gaps = 3/64 (4%) Frame = +1 Query: 388 SRQFV-RRYALPEGAAPETVESRLSSDGVLTITAPRKVPDAXKGERK--VPIAQTGPVRK 558 S +FV R + L E + V S+ + +G++ + A K+P+ KG+RK V P+ Sbjct: 141 STEFVGRAWDLDETTVKKLVGSQ-TGNGIVKVDASLKMPEPKKGDRKGFVLNCLEAPLDV 199 Query: 559 EIKD 570 +IKD Sbjct: 200 DIKD 203 >At5g18190.1 68418.m02135 protein kinase family protein contains Pfam domains, PF00069: Protein kinase domain Length = 691 Score = 28.3 bits (60), Expect = 5.9 Identities = 11/22 (50%), Positives = 12/22 (54%) Frame = +3 Query: 198 QTVETTRGCIS*PRLQHKGRQG 263 Q T GC P + HKGRQG Sbjct: 185 QVYNTLNGCYGVPAVHHKGRQG 206 >At4g36850.1 68417.m05225 PQ-loop repeat family protein / transmembrane family protein similar to SP|Q10482 Seven transmembrane protein 1 {Schizosaccharomyces pombe}; contains Pfam profile PF04193: PQ loop repeat Length = 374 Score = 28.3 bits (60), Expect = 5.9 Identities = 13/52 (25%), Positives = 30/52 (57%), Gaps = 2/52 (3%) Frame = -1 Query: 602 LFSASWVPSLWSLISLRTGPVCAMGT--FLSPLXASGTFLGAVMVRTPSDDS 453 ++ +P +W ++++ G V + F+ L A+ T++G+++VRT D+ Sbjct: 269 IYMGGRIPQIW--LNIKRGSVEGLNPLMFIFALVANATYVGSILVRTTEWDN 318 >At3g49540.1 68416.m05414 expressed protein Length = 166 Score = 28.3 bits (60), Expect = 5.9 Identities = 19/65 (29%), Positives = 34/65 (52%), Gaps = 4/65 (6%) Frame = +1 Query: 421 EGAAPETVESRLSSDGVLTITAPRKVPDAXKGERKV---PI-AQTGPVRKEIKDQSEGTQ 588 E AA E E+++ + + + AP KV A + E+K P+ A+ PV+ E + E ++ Sbjct: 100 ENAATENAEAKVEA---VAVAAPEKVEVAVEAEKKAEAEPVKAEAEPVKAEAEPVKEESK 156 Query: 589 DAENK 603 E + Sbjct: 157 QEEKE 161 >At1g76770.1 68414.m08934 heat shock protein-related contains similarity to 17.9 kDa heat-shock protein [Helianthus annuus] gi|11990130|emb|CAB55634 Length = 244 Score = 27.9 bits (59), Expect = 7.7 Identities = 11/37 (29%), Positives = 24/37 (64%), Gaps = 1/37 (2%) Frame = +1 Query: 391 RQFVRRYALPEGAAPETVESRLSS-DGVLTITAPRKV 498 ++F + + +P+ + +++R + DG LT+T P+KV Sbjct: 93 KEFKKVFRIPDIVILDKIKARFNEEDGTLTVTMPKKV 129 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,591,309 Number of Sequences: 28952 Number of extensions: 291694 Number of successful extensions: 950 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 912 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 949 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1692519896 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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