Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0572
(750 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identi... 150 1e-36
At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identi... 146 1e-35
At3g03910.1 68416.m00405 glutamate dehydrogenase, putative simil... 144 4e-35
At1g51720.1 68414.m05828 glutamate dehydrogenase, putative simil... 64 1e-10
At5g15780.1 68418.m01845 pollen Ole e 1 allergen and extensin fa... 35 0.066
At1g16710.1 68414.m02003 TAZ zinc finger family protein / zinc f... 32 0.35
At4g16980.1 68417.m02560 arabinogalactan-protein family similar ... 32 0.47
At2g07360.1 68415.m00843 SH3 domain-containing protein contains ... 32 0.47
At1g03990.1 68414.m00385 alcohol oxidase-related low similarity ... 29 3.3
At5g34838.1 68418.m04072 hypothetical protein contains Pfam doma... 29 4.4
At3g48560.1 68416.m05302 acetolactate synthase, chloroplast / ac... 29 4.4
At1g02520.1 68414.m00203 multidrug resistance P-glycoprotein, pu... 29 4.4
At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase / 3-... 28 5.8
At5g46540.1 68418.m05730 ABC transporter family protein contains... 28 7.6
>At5g18170.1 68418.m02133 glutamate dehydrogenase 1 (GDH1) identical
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 150 bits (363), Expect = 1e-36
Identities = 89/202 (44%), Positives = 118/202 (58%)
Frame = +3
Query: 36 EVKALSALMTFKCACVDVPFGGAKAGIKINPKEYSEHELEKITRRFTLELAKKGFIGPGV 215
EV AL+ LMT+K A +P+GGAK GI +P + S ELE++TR FT ++ IG
Sbjct: 79 EVNALAQLMTWKTAVAKIPYGGAKGGIGCDPSKLSISELERLTRVFTQKI--HDLIGIHT 136
Query: 216 DVPAPDMGTGEREMSWIADTYAKTVGFQDINAHACVTGKPINQGGIHGRVSATGRGVFHG 395
DVPAPDMGTG + M+WI D Y+K G+ + A VTGKPI+ GG GR +ATGRGV G
Sbjct: 137 DVPAPDMGTGPQTMAWILDEYSKFHGY----SPAVVTGKPIDLGGSLGRDAATGRGVMFG 192
Query: 396 LENFINEANYMSMIGTTPGWGGKTFIVQGFGNVGLHTCRYLVRAGATCIGVIEHDGSIYN 575
E +NE G T G+ F++QGFGNVG + + G + V + G+I N
Sbjct: 193 TEALLNEH------GKT--ISGQRFVIQGFGNVGSWAAKLISEKGGKIVAVSDITGAIKN 244
Query: 576 PDGINPKALEDYRIXNXTVVGF 641
DGI+ AL + + V GF
Sbjct: 245 KDGIDIPALLKHTKEHRGVKGF 266
>At5g07440.1 68418.m00851 glutamate dehydrogenase 2 (GDH2) identical
to glutamate dehydrogenase 2 (GDH 2) [Arabidopsis
thaliana] SWISS-PROT:Q38946
Length = 411
Score = 146 bits (355), Expect = 1e-35
Identities = 85/189 (44%), Positives = 113/189 (59%)
Frame = +3
Query: 36 EVKALSALMTFKCACVDVPFGGAKAGIKINPKEYSEHELEKITRRFTLELAKKGFIGPGV 215
EV AL+ LMT+K A D+P+GGAK GI +P++ S ELE++TR FT ++ IG
Sbjct: 79 EVNALAQLMTWKTAVADIPYGGAKGGIGCSPRDLSLSELERLTRVFTQKI--HDLIGIHT 136
Query: 216 DVPAPDMGTGEREMSWIADTYAKTVGFQDINAHACVTGKPINQGGIHGRVSATGRGVFHG 395
DVPAPDMGT + M+WI D Y+K G ++ A VTGKPI+ GG GR +ATGRGV
Sbjct: 137 DVPAPDMGTNAQTMAWILDEYSKFHG----HSPAVVTGKPIDLGGSLGREAATGRGVVFA 192
Query: 396 LENFINEANYMSMIGTTPGWGGKTFIVQGFGNVGLHTCRYLVRAGATCIGVIEHDGSIYN 575
E + A Y I G TF++QGFGNVG + + G + V + G+I N
Sbjct: 193 TEALL--AEYGKSI------QGLTFVIQGFGNVGTWAAKLIHEKGGKVVAVSDITGAIRN 244
Query: 576 PDGINPKAL 602
P+GI+ AL
Sbjct: 245 PEGIDINAL 253
>At3g03910.1 68416.m00405 glutamate dehydrogenase, putative similar
to glutamate dehydrogenase 1 (GDH 1) [Arabidopsis
thaliana] SWISS-PROT:Q43314
Length = 411
Score = 144 bits (350), Expect = 4e-35
Identities = 87/202 (43%), Positives = 117/202 (57%)
Frame = +3
Query: 36 EVKALSALMTFKCACVDVPFGGAKAGIKINPKEYSEHELEKITRRFTLELAKKGFIGPGV 215
EV AL+ LMT+K A +P+GGAK GI +P E S ELE++TR FT ++ IG
Sbjct: 79 EVNALAQLMTWKTAVAKIPYGGAKGGIGCDPSELSLSELERLTRVFTQKI--HDLIGIHT 136
Query: 216 DVPAPDMGTGEREMSWIADTYAKTVGFQDINAHACVTGKPINQGGIHGRVSATGRGVFHG 395
DVPAPDMGTG + M+WI D Y+K G ++ A VTGKPI+ GG GR +ATGRGV
Sbjct: 137 DVPAPDMGTGPQTMAWILDEYSKFHG----HSPAVVTGKPIDLGGSLGRDAATGRGVLFA 192
Query: 396 LENFINEANYMSMIGTTPGWGGKTFIVQGFGNVGLHTCRYLVRAGATCIGVIEHDGSIYN 575
E +NE G T G+ F +QGFGNVG + + G + V + G+I N
Sbjct: 193 TEALLNEH------GKT--ISGQRFAIQGFGNVGSWAAKLISDKGGKIVAVSDVTGAIKN 244
Query: 576 PDGINPKALEDYRIXNXTVVGF 641
+GI+ +L ++ N + GF
Sbjct: 245 NNGIDILSLLEHAEENRGIKGF 266
>At1g51720.1 68414.m05828 glutamate dehydrogenase, putative similar
to NADP-specific glutatamate dehydrogenase (NADP-GDH)
SP:P28724 [Giardia lamblia (Giardia intestinalis)]
Length = 637
Score = 63.7 bits (148), Expect = 1e-10
Identities = 47/183 (25%), Positives = 77/183 (42%)
Frame = +3
Query: 42 KALSALMTFKCACVDVPFGGAKAGIKINPKEYSEHELEKITRRFTLELAKKGFIGPGVDV 221
K L T K A GGA G +PK S++E+ + + F E+ + ++GP D+
Sbjct: 292 KFLGFQQTLKNALSPYKLGGASGGSDFDPKGKSDNEIMRFCQSFMNEMYR--YMGPDKDL 349
Query: 222 PAPDMGTGEREMSWIADTYAKTVGFQDINAHACVTGKPINQGGIHGRVSATGRGVFHGLE 401
P+ ++G G REM ++ Y + G TG I R A+G GV +
Sbjct: 350 PSEEVGVGTREMGYLFGQYRRLAG----QFQGSFTGPRIYWAASSLRTEASGYGVVYFAR 405
Query: 402 NFINEANYMSMIGTTPGWGGKTFIVQGFGNVGLHTCRYLVRAGATCIGVIEHDGSIYNPD 581
+ + N G +V G G + +H L+ GA + V + G + + D
Sbjct: 406 LILADMNKEIK--------GLRCVVSGCGKIAMHVVEKLIACGAHPVTVSDSKGYLVDDD 457
Query: 582 GIN 590
G +
Sbjct: 458 GFD 460
>At5g15780.1 68418.m01845 pollen Ole e 1 allergen and extensin
family protein contains Pfam profile PF01190: Pollen
proteins Ole e I family
Length = 401
Score = 34.7 bits (76), Expect = 0.066
Identities = 19/52 (36%), Positives = 27/52 (51%)
Frame = -3
Query: 499 SPTLPKPWTMNVLPPQPGVVPIMLM*LASLMKFSKPWNTPLPVAETLPWMPP 344
+PTLP T+ LPP P + P+ ++ SL + PLP LP +PP
Sbjct: 321 TPTLPPIPTIPTLPPLPVLPPVPIVNPPSLPPPPPSFPVPLPPVPGLPGIPP 372
>At1g16710.1 68414.m02003 TAZ zinc finger family protein / zinc
finger (ZZ type) family protein contains Pfam profiles
PF02135: TAZ zinc finger, PF00569: Zinc finger, ZZ type
Length = 1706
Score = 32.3 bits (70), Expect = 0.35
Identities = 22/86 (25%), Positives = 33/86 (38%), Gaps = 1/86 (1%)
Frame = +3
Query: 186 AKKGFIGPGVDVPAPDMGTGEREMSWIADTYAKTVGFQDINAHACVTGKPINQGGIHGRV 365
A +G + P G + + A VG +I + A TG P+ GG+HG
Sbjct: 149 ANSSSVGTMIPTPGLSQTAGNPNLMVTSSVDATIVGNTNITSTALNTGNPLIAGGMHG-- 206
Query: 366 SATGRGVFHGLENF-INEANYMSMIG 440
G H NF + M+ +G
Sbjct: 207 GNMSNGYQHSSRNFSLGSGGSMTSMG 232
>At4g16980.1 68417.m02560 arabinogalactan-protein family similar to
arabinogalactan protein [Arabidopsis thaliana]
gi|10880495|gb|AAG24277; contains proline-rich extensin
domains, INTERPRO:IPR002965
Length = 164
Score = 31.9 bits (69), Expect = 0.47
Identities = 20/69 (28%), Positives = 32/69 (46%)
Frame = -3
Query: 385 TPLPVAETLPWMPPWLIGLPVTQA*ALMS*KPTVFA*VSAIQDISRSPVPMSGAGTSTPG 206
TP P+ T P MP +P+ +M P+ + D+ P+P + +PG
Sbjct: 68 TPPPMPMTPPPMPMAPPPMPMASP-PMMPMTPSTSPSPLTVPDMPSPPMPSGMESSPSPG 126
Query: 205 PMNPFLASS 179
PM P +A+S
Sbjct: 127 PMPPAMAAS 135
>At2g07360.1 68415.m00843 SH3 domain-containing protein contains Pfam
profile PF00018: SH3 domain
Length = 1196
Score = 31.9 bits (69), Expect = 0.47
Identities = 19/64 (29%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = -2
Query: 503 VESHVTETLDDERLATPTGGCTDHAHVV-GFVDEVLQAVEYAPARG*NSAVDATLVNRFA 327
+ + + +T + T + G TD A V G D + ++ + APA +S +D LVN +A
Sbjct: 910 ISAKLIDTYRTKHNITASTGSTDPAVVATGISDLIYESTQPAPAASNSSGLDDDLVNAWA 969
Query: 326 SDAG 315
++ G
Sbjct: 970 ANLG 973
>At1g03990.1 68414.m00385 alcohol oxidase-related low similarity to
long chain fatty alcohol oxidase from Candida cloacae
[GI:6983581], Candida tropicalis [GI:6983594]; Location
of EST 248L9T7, gb|AA713296
Length = 758
Score = 29.1 bits (62), Expect = 3.3
Identities = 17/41 (41%), Positives = 20/41 (48%), Gaps = 1/41 (2%)
Frame = +3
Query: 492 VGL-HTCRYLVRAGATCIGVIEHDGSIYNPDGINPKALEDY 611
+GL R LV AGA +G DG DGI K LE +
Sbjct: 625 IGLKQALRILVAAGAAEVGTYRSDGQRMKCDGIKQKDLEAF 665
>At5g34838.1 68418.m04072 hypothetical protein contains Pfam domain,
PF04827: Protein of unknown function (DUF635)
Length = 311
Score = 28.7 bits (61), Expect = 4.4
Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 4/34 (11%)
Frame = +2
Query: 197 IHWAWRGCPRS*HG-YRRTR---NVLDRRYLCED 286
+HW W+ CP + G Y RT NV DR + +D
Sbjct: 106 MHWEWKNCPTAWKGQYTRTLNDINVFDRSPVFDD 139
>At3g48560.1 68416.m05302 acetolactate synthase, chloroplast /
acetohydroxy-acid synthase (ALS) nearly identical to
SP|P17597 Acetolactate synthase, chloroplast precursor
(EC 2.2.1.6, formerly EC 4.1.3.18) (Acetohydroxy-acid
synthase) (ALS) {Arabidopsis thaliana}
Length = 670
Score = 28.7 bits (61), Expect = 4.4
Identities = 19/41 (46%), Positives = 25/41 (60%)
Frame = -3
Query: 253 SRSPVPMSGAGTSTPGPMNPFLASSRVKRRVIFSSSCSEYS 131
S+SP+P+S S P +NP +SS +RR I SSS S S
Sbjct: 25 SKSPLPISRF--SLPFSLNPNKSSSSSRRRGIKSSSPSSIS 63
>At1g02520.1 68414.m00203 multidrug resistance P-glycoprotein,
putative similar to multidrug-resistant protein CjMDR1
GI:14715462 from [Coptis japonica]
Length = 1278
Score = 28.7 bits (61), Expect = 4.4
Identities = 19/45 (42%), Positives = 24/45 (53%)
Frame = +3
Query: 234 MGTGEREMSWIADTYAKTVGFQDINAHACVTGKPINQGGIHGRVS 368
M TGER+ + I TY KT+ QDI T N G + GR+S
Sbjct: 125 MITGERQAARIRSTYLKTILRQDIGFFDVET----NTGEVVGRMS 165
>At1g17745.1 68414.m02196 D-3-phosphoglycerate dehydrogenase /
3-PGDH identical to SP|O04130
Length = 624
Score = 28.3 bits (60), Expect = 5.8
Identities = 16/34 (47%), Positives = 17/34 (50%)
Frame = +3
Query: 459 GKTFIVQGFGNVGLHTCRYLVRAGATCIGVIEHD 560
GKT V GFG VG R RA + VI HD
Sbjct: 221 GKTLAVMGFGKVGTEVAR---RAKGLGMTVISHD 251
>At5g46540.1 68418.m05730 ABC transporter family protein contains
Pfam profile: PF00005 ABC transporter; similar to
multidrug-resistant protein CjMDR1 GI:14715462 from
[Coptis japonica]
Length = 1248
Score = 27.9 bits (59), Expect = 7.6
Identities = 31/98 (31%), Positives = 43/98 (43%), Gaps = 5/98 (5%)
Frame = +3
Query: 90 PFGGAKAGIKINPKEYSEH-----ELEKITRRFTLELAKKGFIGPGVDVPAPDMGTGERE 254
PF G IN +S+H E+ K+ +F A G + + V M TGER+
Sbjct: 50 PFMSILMGQLINVFGFSDHDHVFKEVSKVAVKFLYLAAYAGVVS-FLQVSCW-MVTGERQ 107
Query: 255 MSWIADTYAKTVGFQDINAHACVTGKPINQGGIHGRVS 368
+ I Y KT+ QDI T N G + GR+S
Sbjct: 108 STRIRRLYLKTILRQDIGFFDTET----NTGEVIGRMS 141
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,238,342
Number of Sequences: 28952
Number of extensions: 330745
Number of successful extensions: 1030
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 976
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1018
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -