Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0542
(700 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g18180.1 68415.m02115 SEC14 cytosolic factor, putative / phos... 35 0.045
At4g39180.1 68417.m05548 SEC14 cytosolic factor, putative / phos... 33 0.14
At4g36490.1 68417.m05181 SEC14 cytosolic factor, putative / phos... 33 0.14
At2g21540.1 68415.m02563 SEC14 cytosolic factor, putative / phos... 33 0.24
At2g18070.1 68415.m02101 hypothetical protein 31 0.73
At4g34580.1 68417.m04913 SEC14 cytosolic factor, putative / phos... 31 0.97
At4g39170.1 68417.m05547 SEC14 cytosolic factor, putative / phos... 30 1.3
At4g16920.1 68417.m02552 disease resistance protein (TIR-NBS-LRR... 29 2.2
At3g13062.3 68416.m01630 expressed protein weak similarity to SP... 29 3.9
At3g13062.2 68416.m01631 expressed protein weak similarity to SP... 29 3.9
At3g13062.1 68416.m01629 expressed protein weak similarity to SP... 29 3.9
At4g20160.1 68417.m02949 expressed protein ; expression supporte... 28 5.2
At3g27530.1 68416.m03441 vesicle tethering family protein contai... 28 5.2
At5g56160.1 68418.m07006 SEC14 cytosolic factor family protein /... 28 6.8
At4g37370.1 68417.m05292 cytochrome P450, putative similar to Cy... 28 6.8
At1g01630.1 68414.m00080 SEC14 cytosolic factor, putative / phos... 27 9.0
>At2g18180.1 68415.m02115 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative contains
Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminussimilar to phosphatidylinositol
transfer-like protein IV (GI:14486707) [Lotus
japonicus];
Length = 558
Score = 35.1 bits (77), Expect = 0.045
Identities = 26/79 (32%), Positives = 34/79 (43%), Gaps = 1/79 (1%)
Frame = +2
Query: 290 LPDEFDDQRIMT-FLRGCKFSLEKTKRKLDMYFTMRTAVPEFFTNRDVKRPELQEILSLV 466
LPD+ DD +M FL+ KF LEKT + R D + E+ E+L
Sbjct: 72 LPDKHDDYHMMLRFLKARKFDLEKTNQMWSDMLRWRKEFGADTVMEDFEFKEIDEVLK-Y 130
Query: 467 QTPALPGITPEGRRVVIMR 523
G+ EGR V I R
Sbjct: 131 YPQGHHGVDKEGRPVYIER 149
>At4g39180.1 68417.m05548 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative
phosphatidylinositol-phosphatidylcholine transfer
protein SEC14, Yarrowia lipolytica, PIR2:S43745;
contains Pfam PF00650 : CRAL/TRIO domain; contains Pfam
PF03765 : CRAL/TRIO, N-terminus
Length = 554
Score = 33.5 bits (73), Expect = 0.14
Identities = 24/79 (30%), Positives = 33/79 (41%), Gaps = 1/79 (1%)
Frame = +2
Query: 290 LPDEFDDQRIMT-FLRGCKFSLEKTKRKLDMYFTMRTAVPEFFTNRDVKRPELQEILSLV 466
LP + DD +M FLR KF LEK K+ R D E++E++
Sbjct: 87 LPSKHDDHHMMLRFLRARKFDLEKAKQMWSDMLNWRKEYGADTIMEDFDFKEIEEVVK-Y 145
Query: 467 QTPALPGITPEGRRVVIMR 523
G+ EGR + I R
Sbjct: 146 YPQGYHGVDKEGRPIYIER 164
>At4g36490.1 68417.m05181 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus]; contains Pfam PF00650 :
CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO,
N-terminus; supporting cDNA gi|23463078|gb|BT000834.1|
Length = 543
Score = 33.5 bits (73), Expect = 0.14
Identities = 30/108 (27%), Positives = 48/108 (44%), Gaps = 1/108 (0%)
Frame = +2
Query: 203 KIREELNENVETRDSDLAHIKEWLKKEPHLPDEFDDQRIMT-FLRGCKFSLEKTKRKLDM 379
+I E++++ E + D ++ L + LP++ DD +M FL+ KF LEKTK+
Sbjct: 42 EIIEDVHDAEELKAVDA--FRQSLILDELLPEKHDDYHMMLRFLKARKFDLEKTKQMWTE 99
Query: 380 YFTMRTAVPEFFTNRDVKRPELQEILSLVQTPALPGITPEGRRVVIMR 523
R + E+ E+L G+ EGR V I R
Sbjct: 100 MLRWRKEFGADTVMEEFDFKEIDEVLK-YYPQGHHGVDKEGRPVYIER 146
>At2g21540.1 68415.m02563 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus]; contains Pfam PF00650
: CRAL/TRIO domain; contains Pfam PF03765 : CRAL/TRIO,
N-terminus
Length = 548
Score = 32.7 bits (71), Expect = 0.24
Identities = 28/81 (34%), Positives = 38/81 (46%), Gaps = 3/81 (3%)
Frame = +2
Query: 290 LPDEFDDQRIMT-FLRGCKFSLEKTKRK-LDM-YFTMRTAVPEFFTNRDVKRPELQEILS 460
LP + DD +M FLR KF LEK K+ DM ++ V + D K E+ E+L
Sbjct: 86 LPSKHDDHHMMLRFLRARKFDLEKAKQMWTDMIHWRKEFGVDTIMEDFDFK--EIDEVLK 143
Query: 461 LVQTPALPGITPEGRRVVIMR 523
G+ +GR V I R
Sbjct: 144 -YYPQGYHGVDKDGRPVYIER 163
>At2g18070.1 68415.m02101 hypothetical protein
Length = 145
Score = 31.1 bits (67), Expect = 0.73
Identities = 20/52 (38%), Positives = 27/52 (51%)
Frame = +2
Query: 146 REPNEAKMTLEQPAGEMWQKIREELNENVETRDSDLAHIKEWLKKEPHLPDE 301
R+P + + QP GE + EE N NVE R+ +L KE K +P DE
Sbjct: 35 RKPADQAASGNQPTGEDVFRSFEEKNANVEVRNENL-KAKESKKSKPKERDE 85
>At4g34580.1 68417.m04913 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein III
(GI:14486705) [Lotus japonicus]; similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus]; similar SEC14 protein,
Saccharomyces cerevisiae, PIR2:A30106; contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus
Length = 560
Score = 30.7 bits (66), Expect = 0.97
Identities = 27/104 (25%), Positives = 43/104 (41%), Gaps = 2/104 (1%)
Frame = +2
Query: 218 LNENVETRDSD-LAHIKEWLKKEPHLPDEFDDQRIMT-FLRGCKFSLEKTKRKLDMYFTM 391
+ ++++ D L ++ L + LP + DD +M FLR KF +EK K+
Sbjct: 54 IEDDIDAEDLQALDAFRQALILDELLPSKLDDLHMMLRFLRARKFDIEKAKQMWSDMIQW 113
Query: 392 RTAVPEFFTNRDVKRPELQEILSLVQTPALPGITPEGRRVVIMR 523
R D E+ E++ G+ EGR V I R
Sbjct: 114 RKDFGADTIIEDFDFEEIDEVMK-HYPQGYHGVDKEGRPVYIER 156
>At4g39170.1 68417.m05547 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative similar to
phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus] and
phosphatidylinositol-phosphatidylcholine transfer
protein SEC14, Yarrowia lipolytica, PIR2:S43745;contains
Pfam PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus
Length = 614
Score = 30.3 bits (65), Expect = 1.3
Identities = 29/91 (31%), Positives = 42/91 (46%), Gaps = 4/91 (4%)
Frame = +2
Query: 263 KEWLKKEPHLPDEFDDQRIMT-FLRGCKFSLEKTKRKLDMYFTMRTAVPEFFTN---RDV 430
++ L E LP + DD +M FL+ KF +EK K M+ M EF T+ +D
Sbjct: 90 RQALVMEELLPHKHDDYHMMLRFLKARKFDIEKAKH---MWADMIQWRKEFGTDTIIQDF 146
Query: 431 KRPELQEILSLVQTPALPGITPEGRRVVIMR 523
+ E+ E+L + EGR V I R
Sbjct: 147 QFEEIDEVLK-YYPHGYHSVDKEGRPVYIER 176
>At4g16920.1 68417.m02552 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1304
Score = 29.5 bits (63), Expect = 2.2
Identities = 29/96 (30%), Positives = 45/96 (46%), Gaps = 1/96 (1%)
Frame = +2
Query: 218 LNENVETRDSDLAHIKEWLKKEPHLPDEFDDQRIMTFLRGCKFSLEKTKRKLDMYFTMRT 397
L +++ RD D EW+K P L ++ DD +I LR C L K R+L F
Sbjct: 382 LGSSLKGRDKD-----EWVKMMPRLRNDSDD-KIEETLRVCYDRLNKKNREL---FKCIA 432
Query: 398 AVPEFFTNRDVKRPELQEI-LSLVQTPALPGITPEG 502
F +VK ++ L+++ +L ITP+G
Sbjct: 433 CFFNGFKVSNVKELLEDDVGLTMLVEKSLIRITPDG 468
>At3g13062.3 68416.m01630 expressed protein weak similarity to
SP|Q9UKL6 Phosphatidylcholine transfer protein (PC-TP)
{Homo sapiens}
Length = 411
Score = 28.7 bits (61), Expect = 3.9
Identities = 21/68 (30%), Positives = 30/68 (44%), Gaps = 2/68 (2%)
Frame = -3
Query: 347 RTYNLGGRS*CAGHRIHQVDAVPSSATP*CGRGRSLWS--PRFRSAPRGSSATFHRLAAP 174
R + GR+ C H +HQ DA + R +WS + +A R AT HR P
Sbjct: 246 RIRKVPGRNACEIHMVHQEDAGLNVEMAKLAFSRGIWSYVCKMENALRKYIATSHRPQGP 305
Query: 173 ESSWLRLV 150
S + L+
Sbjct: 306 TLSAVSLM 313
>At3g13062.2 68416.m01631 expressed protein weak similarity to
SP|Q9UKL6 Phosphatidylcholine transfer protein (PC-TP)
{Homo sapiens}
Length = 440
Score = 28.7 bits (61), Expect = 3.9
Identities = 21/68 (30%), Positives = 30/68 (44%), Gaps = 2/68 (2%)
Frame = -3
Query: 347 RTYNLGGRS*CAGHRIHQVDAVPSSATP*CGRGRSLWS--PRFRSAPRGSSATFHRLAAP 174
R + GR+ C H +HQ DA + R +WS + +A R AT HR P
Sbjct: 238 RIRKVPGRNACEIHMVHQEDAGLNVEMAKLAFSRGIWSYVCKMENALRKYIATSHRPQGP 297
Query: 173 ESSWLRLV 150
S + L+
Sbjct: 298 TLSAVSLM 305
>At3g13062.1 68416.m01629 expressed protein weak similarity to
SP|Q9UKL6 Phosphatidylcholine transfer protein (PC-TP)
{Homo sapiens}
Length = 403
Score = 28.7 bits (61), Expect = 3.9
Identities = 21/68 (30%), Positives = 30/68 (44%), Gaps = 2/68 (2%)
Frame = -3
Query: 347 RTYNLGGRS*CAGHRIHQVDAVPSSATP*CGRGRSLWS--PRFRSAPRGSSATFHRLAAP 174
R + GR+ C H +HQ DA + R +WS + +A R AT HR P
Sbjct: 238 RIRKVPGRNACEIHMVHQEDAGLNVEMAKLAFSRGIWSYVCKMENALRKYIATSHRPQGP 297
Query: 173 ESSWLRLV 150
S + L+
Sbjct: 298 TLSAVSLM 305
>At4g20160.1 68417.m02949 expressed protein ; expression supported
by MPSS
Length = 1188
Score = 28.3 bits (60), Expect = 5.2
Identities = 15/44 (34%), Positives = 27/44 (61%), Gaps = 1/44 (2%)
Frame = +2
Query: 143 YREPNEAKMTLEQPAGEMWQKI-REELNENVETRDSDLAHIKEW 271
+R PN+ K +++P E +K+ REE ENVE ++ + + +W
Sbjct: 874 HRNPNDKK-GIKEPEDEESKKVEREETGENVE--EASVEFVNDW 914
>At3g27530.1 68416.m03441 vesicle tethering family protein contains
Pfam PF04869: Uso1 / p115 like vesicle tethering protein,
head region and PF04871: Uso1 / p115 like vesicle
tethering protein, C terminal region
Length = 914
Score = 28.3 bits (60), Expect = 5.2
Identities = 20/87 (22%), Positives = 41/87 (47%)
Frame = +2
Query: 113 QTLASDLIFEYREPNEAKMTLEQPAGEMWQKIREELNENVETRDSDLAHIKEWLKKEPHL 292
+ +A+ L F+ + ++A +LEQ + Q+++ + D+ IKE ++KE
Sbjct: 801 KNMAAKLEFDLKSLSDAYNSLEQANYHLEQEVKSLKGGESPMQFPDIEAIKEEVRKEAQK 860
Query: 293 PDEFDDQRIMTFLRGCKFSLEKTKRKL 373
E + ++ L + +EK KL
Sbjct: 861 ESEDELNDLLVCLGQEESKVEKLSAKL 887
>At5g56160.1 68418.m07006 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
similar to phosphatidylinositol transfer-like protein IV
(GI:14486707) [Lotus japonicus] and SEC14 cytosolic
factor (SP:P45816) [Candida lipolytica]
Length = 592
Score = 27.9 bits (59), Expect = 6.8
Identities = 23/100 (23%), Positives = 47/100 (47%), Gaps = 3/100 (3%)
Frame = +2
Query: 233 ETRDSDLAHIKEWLKKEPHLPDEFDDQR-IMTFLRGCKFSLEKTKRKLDMYFTMRTAVPE 409
E + ++ +++ L ++ LP DD ++ FL+ +F +EKT + R E
Sbjct: 81 EKEEKLVSKLRQQLLQKDLLPPVHDDYHMLLRFLKTMEFKIEKTVTAWEEMLKWR---KE 137
Query: 410 FFTNRDVKRPELQEILSLVQ--TPALPGITPEGRRVVIMR 523
F T+R ++ +E+ + + G+ +GR + I R
Sbjct: 138 FGTDRIIQDFNFKELDEVTRHYPQGYHGVDKDGRPIYIER 177
>At4g37370.1 68417.m05292 cytochrome P450, putative similar to
Cytochrome P450 91A1 (SP:Q9FG65 )[Arabidopsis thaliana];
cytochrome P450, Glycyrrhiza echinata, AB001379
Length = 497
Score = 27.9 bits (59), Expect = 6.8
Identities = 14/43 (32%), Positives = 24/43 (55%)
Frame = +2
Query: 353 EKTKRKLDMYFTMRTAVPEFFTNRDVKRPELQEILSLVQTPAL 481
EK +D T++ + P++FT+R +K L IL+ T A+
Sbjct: 264 EKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGTDTSAV 306
>At1g01630.1 68414.m00080 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative contains
Pfam PF00650 : CRAL/TRIO domain and PF03765 : CRAL/TRIO,
N-terminus; similar to polyphosphoinositide binding
protein Ssh2p GB:AAB94599 GI:2739046 from [Glycine max]
Length = 255
Score = 27.5 bits (58), Expect = 9.0
Identities = 20/82 (24%), Positives = 34/82 (41%)
Frame = +2
Query: 158 EAKMTLEQPAGEMWQKIREELNENVETRDSDLAHIKEWLKKEPHLPDEFDDQRIMTFLRG 337
E K T ++PA QK + + +E S + ++ ++ E DD I FLR
Sbjct: 2 ENKETKQEPAAAAEQKTVPLIEDEIER--SKVGIMRALCDRQDPETKEVDDLMIRRFLRA 59
Query: 338 CKFSLEKTKRKLDMYFTMRTAV 403
+EK Y T + ++
Sbjct: 60 RDLDIEKASTMFLNYLTWKRSM 81
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,162,667
Number of Sequences: 28952
Number of extensions: 319821
Number of successful extensions: 992
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 960
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 992
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -