Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0513
(749 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces po... 328 4e-91
SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces p... 43 5e-05
SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc... 36 0.008
SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar ... 29 0.71
SPBPB8B6.03 ||SPAPB8B6.03, SPAPB8B6.03|acetamidase |Schizosaccha... 29 0.71
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 29 0.94
SPAC15A10.01 |atm1|SPAC8C9.18|ABC family iron transporter Atm1|S... 27 2.2
SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 27 3.8
SPAC14C4.14 |atp1||F1-ATPase alpha subunit|Schizosaccharomyces p... 27 3.8
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 26 5.0
SPCC553.06 |||oligosaccharyltransferase subunit|Schizosaccharomy... 26 5.0
SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces pom... 26 6.6
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 26 6.6
SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11 |Schizosacc... 26 6.6
SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1 |Schizosaccharom... 25 8.7
>SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 619
Score = 328 bits (807), Expect = 4e-91
Identities = 149/231 (64%), Positives = 188/231 (81%), Gaps = 2/231 (0%)
Frame = +1
Query: 13 IIRLEGDMATIQVYEETSGVTVGDPVLRTGKPLSVELGPGILGSIFDGIQRPLKDINELT 192
+IR+ D TIQVYEETSG+TVGDPV RTGKPLSVELGPG+ +I+DGIQRPLK I + +
Sbjct: 61 VIRIHQDKCTIQVYEETSGLTVGDPVQRTGKPLSVELGPGLAETIYDGIQRPLKQIFDKS 120
Query: 193 QSIYIPKGINVPSLAREVDWEFNP-LNVKVGSHITGGDLYGIVHENTLVK-HRMLVPPKA 366
QSIYIP+GIN SL RE W+F P ++++G H++GGD++G V EN+L H++++PP+A
Sbjct: 121 QSIYIPRGINTESLNREHKWDFTPNKDLRIGDHVSGGDVFGSVFENSLFNDHKIMLPPRA 180
Query: 367 KGTVTYIAPAGNYKVTDVVLETEFDGERQKYSMLQVWPVRQPRPVTEKLPANHPLLTGQR 546
+GTVTYIA AG+Y V + +LE EF+G++ +SML WPVR RPV + L AN PLLTGQR
Sbjct: 181 RGTVTYIAEAGSYHVDEKLLEVEFNGKKHSFSMLHTWPVRAARPVADNLTANQPLLTGQR 240
Query: 547 VLDSLFPCVQGGTTAIPGAFGCGKTVISQALSKYXNFDVIIYXGCGERXNQ 699
VLD+L+PCVQGGTTAIPGAFGCGKTVISQ+LSKY N D+I+Y GCGER N+
Sbjct: 241 VLDALYPCVQGGTTAIPGAFGCGKTVISQSLSKYSNSDLIVYVGCGERGNE 291
>SPAC222.12c |atp2||F1-ATPase beta subunit |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 525
Score = 42.7 bits (96), Expect = 5e-05
Identities = 23/73 (31%), Positives = 37/73 (50%)
Frame = +1
Query: 25 EGDMATIQVYEETSGVTVGDPVLRTGKPLSVELGPGILGSIFDGIQRPLKDINELTQSIY 204
E + TI + + T G+ G V+ TG P+S+ +GPG LG I + I P+ + + Y
Sbjct: 102 ENTVRTIAM-DGTEGLVRGTAVIDTGSPISIPVGPGTLGRIMNVIGEPVDERGPIKAVKY 160
Query: 205 IPKGINVPSLARE 243
P + PS +
Sbjct: 161 SPIHADAPSFEEQ 173
Score = 35.9 bits (79), Expect = 0.006
Identities = 26/89 (29%), Positives = 39/89 (43%), Gaps = 4/89 (4%)
Frame = +1
Query: 445 ERQKYSMLQVWPVRQPRPVTEKLPANHPLL-TGQRVLDSLFPCVQGGTTAIPGAFGCGKT 621
ER ++ P+ P E+ +L TG +V+D L P +GG + G G GKT
Sbjct: 151 ERGPIKAVKYSPIHADAPSFEEQSTTPEILETGIKVVDLLAPYARGGKIGLFGGAGVGKT 210
Query: 622 VISQALSK---YXNFDVIIYXGCGERXNQ 699
V Q L + ++ G GER +
Sbjct: 211 VFIQELINNIAKAHGGYSVFTGVGERTRE 239
>SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 503
Score = 35.5 bits (78), Expect = 0.008
Identities = 23/78 (29%), Positives = 38/78 (48%), Gaps = 2/78 (2%)
Frame = +1
Query: 13 IIRLEGDMATIQVYEETSGVTVGDPVLR-TGKPLSVELGPGILGSIFDGIQRPL-KDINE 186
++ + G A +QV+E TSGV V + TG + + + +LG +F+G P+ K N
Sbjct: 63 VLEVAGHKAIVQVFEGTSGVDVRKTTIDFTGHSMRIPVSEDMLGRVFNGSGLPIDKGPNL 122
Query: 187 LTQSIYIPKGINVPSLAR 240
L + G + AR
Sbjct: 123 LAEDYLDINGSPINPYAR 140
>SPCC737.09c |hmt1|SPCC74.08c|ATP-binding cassette-type vacuolar
membrane transporter Hmt1|Schizosaccharomyces pombe|chr
3|||Manual
Length = 830
Score = 29.1 bits (62), Expect = 0.71
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -2
Query: 91 VLDHLQLHLKFLRIPGWWPC 32
V+D L + LR+P WWPC
Sbjct: 67 VVDISNLVIYALRVPNWWPC 86
>SPBPB8B6.03 ||SPAPB8B6.03, SPAPB8B6.03|acetamidase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 547
Score = 29.1 bits (62), Expect = 0.71
Identities = 25/108 (23%), Positives = 52/108 (48%), Gaps = 2/108 (1%)
Frame = +1
Query: 124 GPGILGSIFDGIQRPLKDINELTQSIYIPKGINVPSLAREVDWEFNPLNVKVGSHITGGD 303
G ++ S+ + R LKDI T+++ + S A ++D + P+ K SH+
Sbjct: 264 GQELIPSVIGPMARSLKDIEFFTETV-------IASEAWKIDSKLLPIPWKNQSHLKSKK 316
Query: 304 L-YGIVHENTLVK-HRMLVPPKAKGTVTYIAPAGNYKVTDVVLETEFD 441
L +G++ + +VK H ++ +A V + Y+V ++ + + D
Sbjct: 317 LIFGVLKTDGIVKPHPPII--RALNEVVAVLEKSGYEVIEISIPFQKD 362
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 28.7 bits (61), Expect = 0.94
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -1
Query: 158 IPSKMEPKIPGPSSTDKGFPVRSTGSPTVTPE 63
IPS + P P T GFP R PT+ P+
Sbjct: 368 IPSTLPPADGVPKPTPDGFPRRFERDPTIPPD 399
>SPAC15A10.01 |atm1|SPAC8C9.18|ABC family iron transporter
Atm1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 693
Score = 27.5 bits (58), Expect = 2.2
Identities = 16/47 (34%), Positives = 22/47 (46%)
Frame = +1
Query: 520 NHPLLTGQRVLDSLFPCVQGGTTAIPGAFGCGKTVISQALSKYXNFD 660
N P+L G F G A GA GCGK+ I + L ++ + D
Sbjct: 455 NRPILNG-----CSFNIPAGAKVAFVGASGCGKSTILRLLFRFYDTD 496
Score = 25.4 bits (53), Expect = 8.7
Identities = 12/43 (27%), Positives = 23/43 (53%)
Frame = +1
Query: 61 TSGVTVGDPVLRTGKPLSVELGPGILGSIFDGIQRPLKDINEL 189
TS +TVGD V+ + + LGS++ +++ D+ +L
Sbjct: 375 TSNLTVGDLVMINQLVFQLSIPLNFLGSVYREMRQAFTDMEQL 417
>SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 4717
Score = 26.6 bits (56), Expect = 3.8
Identities = 13/41 (31%), Positives = 22/41 (53%)
Frame = +1
Query: 598 GAFGCGKTVISQALSKYXNFDVIIYXGCGERXNQNVRGAPR 720
G GCGKT + Q L++ + ++ I + N ++ GA R
Sbjct: 1193 GDTGCGKTTVCQILAECLHKELHIINAHQDTENGDIIGAQR 1233
>SPAC14C4.14 |atp1||F1-ATPase alpha subunit|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 536
Score = 26.6 bits (56), Expect = 3.8
Identities = 13/35 (37%), Positives = 19/35 (54%)
Frame = +1
Query: 526 PLLTGQRVLDSLFPCVQGGTTAIPGAFGCGKTVIS 630
P+ TG + +DS+ P +G I G GKT I+
Sbjct: 173 PMQTGLKAIDSMVPIGRGQRELIIGDRQTGKTAIA 207
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 26.2 bits (55), Expect = 5.0
Identities = 12/33 (36%), Positives = 20/33 (60%), Gaps = 1/33 (3%)
Frame = +1
Query: 25 EGDMATIQVYEETS-GVTVGDPVLRTGKPLSVE 120
E D A++ + T+ +T DPV + KPL++E
Sbjct: 572 ENDKASVSITTTTTPSITPADPVPTSPKPLAIE 604
>SPCC553.06 |||oligosaccharyltransferase subunit|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 271
Score = 26.2 bits (55), Expect = 5.0
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = +1
Query: 532 LTGQRVLDSLFPCVQGGTTAIP 597
LTG L+ F C+ GT AIP
Sbjct: 56 LTGDETLNLKFTCILNGTGAIP 77
>SPBC19C7.03 |cyr1|git2|adenylate cyclase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1692
Score = 25.8 bits (54), Expect = 6.6
Identities = 12/26 (46%), Positives = 16/26 (61%)
Frame = -1
Query: 95 RSTGSPTVTPEVSSYTWMVAMSPSRR 18
RS G+PT PE +YT + + S S R
Sbjct: 1553 RSLGTPTALPETQTYTPVRSRSNSLR 1578
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 25.8 bits (54), Expect = 6.6
Identities = 15/44 (34%), Positives = 18/44 (40%)
Frame = -1
Query: 377 TVPLALGGTNILCLTRVFSCTIPYKSPPVMWDPTLTFNGLNSQS 246
TVP T + S ++PY S PV P T N S S
Sbjct: 412 TVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455
>SPBC646.02 |cwf11||complexed with Cdc5 protein Cwf11
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1284
Score = 25.8 bits (54), Expect = 6.6
Identities = 13/39 (33%), Positives = 19/39 (48%)
Frame = +1
Query: 511 LPANHPLLTGQRVLDSLFPCVQGGTTAIPGAFGCGKTVI 627
LP+N + L+S+ Q G T + G CGK V+
Sbjct: 719 LPSNRLYTYNDKQLESILRGSQPGLTMVNGPTRCGKHVL 757
>SPAC17G8.01c |trl1|SPAC6C3.10c|tRNA ligase Trl1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 25.4 bits (53), Expect = 8.7
Identities = 12/34 (35%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = +1
Query: 568 CVQGGTTAIPGA-FGCGKTVISQALSKYXNFDVI 666
C + T +P A GCGKT +++ L K + V+
Sbjct: 381 CGKEFTLLVPIATIGCGKTTVAKILEKLFGWPVV 414
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.140 0.421
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,995,735
Number of Sequences: 5004
Number of extensions: 64155
Number of successful extensions: 201
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 188
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 199
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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