Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0503
(750 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 423 e-121
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 413 e-117
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 305 3e-85
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 272 2e-75
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 36 4e-04
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.3
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.1
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.0
AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 9.3
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 423 bits (1043), Expect = e-121
Identities = 201/215 (93%), Positives = 204/215 (94%)
Frame = +2
Query: 68 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 247
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 248 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 427
DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120
Query: 428 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 607
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180
Query: 608 IGYNPXAVAFVPISGWHGRQHVWSLQPKCLWFKGW 712
IGYNP AVAFVPISGWHG ++ + K WFKGW
Sbjct: 181 IGYNPAAVAFVPISGWHG-DNMLEVSSKMPWFKGW 214
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 413 bits (1016), Expect = e-117
Identities = 195/215 (90%), Positives = 201/215 (93%)
Frame = +2
Query: 68 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 247
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 248 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 427
DKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG
Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120
Query: 428 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 607
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180
Query: 608 IGYNPXAVAFVPISGWHGRQHVWSLQPKCLWFKGW 712
IGYN +VAFVPISGWHG ++ PK W+KGW
Sbjct: 181 IGYNTASVAFVPISGWHG-DNMLEPSPKTPWYKGW 214
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 305 bits (749), Expect = 3e-85
Identities = 145/158 (91%), Positives = 148/158 (93%)
Frame = +2
Query: 239 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 418
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 419 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 598
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 599 IKKIGYNPXAVAFVPISGWHGRQHVWSLQPKCLWFKGW 712
IKKIGYNP AVAFVPISGWHG ++ + K WFKGW
Sbjct: 121 IKKIGYNPAAVAFVPISGWHG-DNMLEVSSKMPWFKGW 157
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 272 bits (667), Expect = 2e-75
Identities = 129/142 (90%), Positives = 132/142 (92%)
Frame = +2
Query: 287 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 466
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 467 REHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPXAVAFVPI 646
REHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNP AVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 647 SGWHGRQHVWSLQPKCLWFKGW 712
SGWHG ++ + K WFKGW
Sbjct: 121 SGWHG-DNMLEVSSKMPWFKGW 141
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 35.9 bits (79), Expect = 4e-04
Identities = 33/114 (28%), Positives = 50/114 (43%)
Frame = +2
Query: 326 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 505
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 506 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPXAVAFVPISGWHGRQ 667
+IV +NK+D + ++E K + I+++G V + G + R+
Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELGGEIQCVKISALKGINLRE 298
Score = 25.4 bits (53), Expect = 0.57
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 80 KTHINIVVIGHVDSGKST 133
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.3
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +2
Query: 89 INIVVIGHVDSGKST 133
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.1
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 311 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 412
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.0
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 311 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 412
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase
protein.
Length = 580
Score = 21.4 bits (43), Expect = 9.3
Identities = 7/20 (35%), Positives = 13/20 (65%)
Frame = +3
Query: 531 WIPLNHHTVSPDLRKSRRKY 590
W+P+N + S +L +R+Y
Sbjct: 443 WLPVNENYKSLNLAAQKREY 462
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 216,083
Number of Sequences: 438
Number of extensions: 4255
Number of successful extensions: 17
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 15
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 16
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 23510295
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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