Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0503
(750 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 347 5e-96
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 347 5e-96
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 347 5e-96
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 347 5e-96
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 197 6e-51
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 193 9e-50
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 102 2e-22
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 97 1e-20
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 56 2e-08
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 56 2e-08
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 52 3e-07
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 52 4e-07
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 52 5e-07
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 48 9e-06
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 43 3e-04
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 43 3e-04
At5g13650.2 68418.m01585 elongation factor family protein contai... 42 3e-04
At5g13650.1 68418.m01584 elongation factor family protein contai... 42 3e-04
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 41 0.001
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 40 0.001
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 39 0.003
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 37 0.016
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 37 0.016
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 36 0.038
At3g01360.1 68416.m00057 expressed protein contains Pfam profile... 33 0.27
At5g46180.1 68418.m05680 ornithine aminotransferase, putative / ... 30 1.4
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.4
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 30 1.4
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.9
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.9
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 29 2.5
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 5.8
At1g26130.1 68414.m03190 haloacid dehalogenase-like hydrolase fa... 28 7.6
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 347 bits (853), Expect = 5e-96
Identities = 165/214 (77%), Positives = 183/214 (85%)
Frame = +2
Query: 68 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 247
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 248 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 427
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 428 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 607
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 608 IGYNPXAVAFVPISGWHGRQHVWSLQPKCLWFKG 709
+GYNP + FVPISG+ G ++ W+KG
Sbjct: 181 VGYNPDKIPFVPISGFEG-DNMIERSTNLDWYKG 213
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 347 bits (853), Expect = 5e-96
Identities = 165/214 (77%), Positives = 183/214 (85%)
Frame = +2
Query: 68 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 247
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 248 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 427
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 428 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 607
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 608 IGYNPXAVAFVPISGWHGRQHVWSLQPKCLWFKG 709
+GYNP + FVPISG+ G ++ W+KG
Sbjct: 181 VGYNPDKIPFVPISGFEG-DNMIERSTNLDWYKG 213
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 347 bits (853), Expect = 5e-96
Identities = 165/214 (77%), Positives = 183/214 (85%)
Frame = +2
Query: 68 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 247
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 248 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 427
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 428 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 607
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 608 IGYNPXAVAFVPISGWHGRQHVWSLQPKCLWFKG 709
+GYNP + FVPISG+ G ++ W+KG
Sbjct: 181 VGYNPDKIPFVPISGFEG-DNMIERSTNLDWYKG 213
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 347 bits (853), Expect = 5e-96
Identities = 165/214 (77%), Positives = 183/214 (85%)
Frame = +2
Query: 68 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 247
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 248 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 427
DKLKAERERGITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 428 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 607
G FEAGISK+GQTREHALLAFTLGVKQ+I NKMD+T P YS+ R++EI KEVSSY+KK
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKK 180
Query: 608 IGYNPXAVAFVPISGWHGRQHVWSLQPKCLWFKG 709
+GYNP + FVPISG+ G ++ W+KG
Sbjct: 181 VGYNPDKIPFVPISGFEG-DNMIERSTNLDWYKG 213
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 197 bits (481), Expect = 6e-51
Identities = 95/213 (44%), Positives = 141/213 (66%), Gaps = 2/213 (0%)
Frame = +2
Query: 77 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 256
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 257 KAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 436
+ ER +G T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEF
Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEF 217
Query: 437 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGY 616
E G + GQTREH LA TLGV +LIV VNKMD +S+ R++EI++++ ++K GY
Sbjct: 218 ETGYERGGQTREHVQLAKTLGVSKLIVVVNKMDDPTVNWSKERYDEIEQKMVPFLKASGY 277
Query: 617 NPXA-VAFVPISGWHGRQHVWSL-QPKCLWFKG 709
N V F+PISG G+ + Q C W+ G
Sbjct: 278 NTKKDVVFLPISGLMGKNMDQRMGQEICPWWSG 310
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 193 bits (471), Expect = 9e-50
Identities = 90/212 (42%), Positives = 140/212 (66%), Gaps = 3/212 (1%)
Frame = +2
Query: 83 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 262
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 263 ERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA 442
ERERGIT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEA
Sbjct: 298 ERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEA 357
Query: 443 GISK-NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYN 619
G GQTREHA + GV+Q+IV +NKMD YS+ RF+ IK+ V S+++ +
Sbjct: 358 GFDNLKGQTREHARVLRGFGVEQVIVAINKMDIV--GYSKERFDLIKQHVGSFLQSCRFK 415
Query: 620 PXAVAFVPISGWHGRQHVWSLQPKCL--WFKG 709
++ ++P+S + V + L W++G
Sbjct: 416 DSSLTWIPLSAMENQNLVAAPSDNRLSSWYQG 447
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 102 bits (245), Expect = 2e-22
Identities = 63/179 (35%), Positives = 93/179 (51%), Gaps = 1/179 (0%)
Frame = +2
Query: 65 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV 244
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE--------------- 116
Query: 245 LDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 424
+D ER RGITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+
Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176
Query: 425 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFE-EIKKEVSSY 598
G QT+EH LLA +GV ++V +NK D + E E+++ +SSY
Sbjct: 177 DGPMP-------QTKEHILLAKQVGVPDMVVFLNKEDQVDDAELLELVELEVRELLSSY 228
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 97.1 bits (231), Expect = 1e-20
Identities = 62/177 (35%), Positives = 90/177 (50%)
Frame = +2
Query: 74 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 253
+ K H+N+ IGHVD GK+T T + K E GK +DK
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLTAAIT---------------KVLAEEGKAKAIAFDEIDK 107
Query: 254 LKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 433
E++RGITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 108 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGP 167
Query: 434 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIK 604
QT+EH LLA +GV L+ +NK+D + P E +E+ S+ K
Sbjct: 168 MP-------QTKEHILLARQVGVPSLVCFLNKVDVVDDPELLELVEMELRELLSFYK 217
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 56.4 bits (130), Expect = 2e-08
Identities = 41/128 (32%), Positives = 61/128 (47%)
Frame = +2
Query: 92 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 271
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 272 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 451
+GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 452 KNGQTREH 475
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 56.4 bits (130), Expect = 2e-08
Identities = 41/128 (32%), Positives = 61/128 (47%)
Frame = +2
Query: 92 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 271
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 272 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 451
+GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 452 KNGQTREH 475
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 52.4 bits (120), Expect = 3e-07
Identities = 52/174 (29%), Positives = 81/174 (46%), Gaps = 4/174 (2%)
Frame = +2
Query: 92 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 271
N +I H+D GKST L+ G + R + K F LD + ERE
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131
Query: 272 RGITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFE 439
RGITI + + +E + + + +ID PGH DF + + + A+L+V A G E
Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG-VE 190
Query: 440 AGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 601
A QT + LA + ++I +NK+D P +EP E++ +E+ I
Sbjct: 191 A------QTLANVYLALENNL-EIIPVLNKIDL---PGAEP--EKVLREIEEVI 232
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 52.0 bits (119), Expect = 4e-07
Identities = 36/113 (31%), Positives = 53/113 (46%)
Frame = +2
Query: 92 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 271
NI ++ HVD GK+T HLI GG + GK F +D L E+
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG--------GVLHPRLAGKLRF-----MDYLDEEQR 57
Query: 272 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 430
R IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 58 RAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 51.6 bits (118), Expect = 5e-07
Identities = 36/117 (30%), Positives = 56/117 (47%)
Frame = +2
Query: 80 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 259
K + NI ++ H+D+GK+TTT ++Y G K E+ +G+ W ++
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTGRNYK---------IGEVHEGTATMDW----ME 140
Query: 260 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 430
E+ERGITI A K+ + IID PGH DF + D A+ + + G
Sbjct: 141 QEQERGITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 47.6 bits (108), Expect = 9e-06
Identities = 57/186 (30%), Positives = 79/186 (42%), Gaps = 9/186 (4%)
Frame = +2
Query: 65 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV 244
K EK N +I H+D GKST L+ G I K G G +Y
Sbjct: 60 KFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK-------------GHGQPQY--- 102
Query: 245 LDKLKAERERGITIDIALWKF---------ETSKYYVTIIDAPGHRDFIKNMITGTSQAD 397
LDKL +RERGIT+ E S Y + +ID PGH DF + S
Sbjct: 103 LDKL--QRERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLSACQ 160
Query: 398 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEI 577
A+L+V A G QT + LAF + ++ +NK+D P ++P E +
Sbjct: 161 GALLVVDAAQG-------VQAQTVANFYLAFEANL-TIVPVINKIDQ---PTADP--ERV 207
Query: 578 KKEVSS 595
K ++ S
Sbjct: 208 KAQLKS 213
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 42.7 bits (96), Expect = 3e-04
Identities = 48/192 (25%), Positives = 85/192 (44%), Gaps = 14/192 (7%)
Frame = +2
Query: 62 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 232
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86
Query: 233 YAWVLDKLK---------AERERGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 379
Y DK + +E T D+ ++ T + +V+ +D PGH + M+
Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146
Query: 380 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE 559
G + D A+L++AA QT EH + +K +I+ NK+D +
Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKIDLINEKAAT 200
Query: 560 PRFEEIKKEVSS 595
+ E I+K +++
Sbjct: 201 EQHEAIQKFITN 212
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 42.7 bits (96), Expect = 3e-04
Identities = 48/192 (25%), Positives = 85/192 (44%), Gaps = 14/192 (7%)
Frame = +2
Query: 62 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 232
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86
Query: 233 YAWVLDKLK---------AERERGITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 379
Y DK + +E T D+ ++ T + +V+ +D PGH + M+
Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146
Query: 380 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE 559
G + D A+L++AA QT EH + +K +I+ NK+D +
Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKIDLINEKAAT 200
Query: 560 PRFEEIKKEVSS 595
+ E I+K +++
Sbjct: 201 EQHEAIQKFITN 212
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 42.3 bits (95), Expect = 3e-04
Identities = 46/159 (28%), Positives = 66/159 (41%)
Frame = +2
Query: 92 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 271
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 85 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 129
Query: 272 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 451
RGITI V IID PGH DF + + D +L+V + G
Sbjct: 130 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 185
Query: 452 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 568
QTR A G ++V VNK+D P + P F
Sbjct: 186 ---QTRFVLKKALEFG-HAVVVVVNKIDR---PSARPEF 217
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 42.3 bits (95), Expect = 3e-04
Identities = 46/159 (28%), Positives = 66/159 (41%)
Frame = +2
Query: 92 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 271
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 84 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 128
Query: 272 RGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 451
RGITI V IID PGH DF + + D +L+V + G
Sbjct: 129 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEGPMP---- 184
Query: 452 KNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRF 568
QTR A G ++V VNK+D P + P F
Sbjct: 185 ---QTRFVLKKALEFG-HAVVVVVNKIDR---PSARPEF 216
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 40.7 bits (91), Expect = 0.001
Identities = 44/186 (23%), Positives = 82/186 (44%), Gaps = 8/186 (4%)
Frame = +2
Query: 62 PKMGKEKTHINIVVIGHVDSGKST----TTG-HLIYKCGGIDKR-TIEKFEKEAQEMGKG 223
P++ + INI IGHV GKST +G H + +++ TI+ A+
Sbjct: 25 PEVISRQATINIGTIGHVAHGKSTIVKAVSGVHTVKFKSELERNITIKLGYANAKIYKCE 84
Query: 224 SFKYAWVLDKLKAERERGITIDIALWKFETSKY--YVTIIDAPGHRDFIKNMITGTSQAD 397
+ +E + D++ + K +V+ +D PGH + M+ G + D
Sbjct: 85 KCPRPVAYKSFGSGKEDNPSCDVSGHEKYKMKLLRHVSFVDCPGHDILMATMLNGAAIMD 144
Query: 398 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEI 577
A+LI+AA QT EH + +K +I+ NK+D + + + E+I
Sbjct: 145 GALLIIAANE------TCPQPQTAEHLASVDMMHLKDIIIIQNKIDLIQENEAIKQHEDI 198
Query: 578 KKEVSS 595
++ +++
Sbjct: 199 QRFITN 204
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 40.3 bits (90), Expect = 0.001
Identities = 28/103 (27%), Positives = 49/103 (47%), Gaps = 4/103 (3%)
Frame = +2
Query: 305 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 472
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 473 HALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 601
H + +K +I+ NK+D + + + E I+K + + +
Sbjct: 166 HLAAVEIMQLKHIIILQNKIDLIQENVAINQHEAIQKFIMNTV 208
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 39.1 bits (87), Expect = 0.003
Identities = 30/112 (26%), Positives = 51/112 (45%), Gaps = 4/112 (3%)
Frame = +2
Query: 92 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRT---IEKFEKEAQEMGKG-SFKYAWVLDKLK 259
N+ VI HVD GKST T L+ G I + + + A E +G + K + +
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGISLYYE 80
Query: 260 AERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 415
E ++ + ++Y + +ID+PGH DF + D A+++V
Sbjct: 81 MTDE---SLKSFTGARDGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.7 bits (81), Expect = 0.016
Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%)
Frame = +2
Query: 92 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 271
N+ ++GH+ GK+ L+ + + K EK KY D E+E
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187
Query: 272 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 430
R I+I + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.7 bits (81), Expect = 0.016
Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%)
Frame = +2
Query: 92 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 271
N+ ++GH+ GK+ L+ + + K EK KY D E+E
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187
Query: 272 RGITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 430
R I+I + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 35.5 bits (78), Expect = 0.038
Identities = 33/105 (31%), Positives = 50/105 (47%), Gaps = 3/105 (2%)
Frame = +2
Query: 326 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 505
+T +D PGH F + G + D VL+VAA G QT E A + V
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDGVMP-------QTLEAIAHARSANV- 321
Query: 506 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSS---YIKKIGYNPXAV 631
++V +NK D P + P E++K +++S ++ IG N AV
Sbjct: 322 PVVVAINKCDK---PGANP--EKVKYQLTSEGIELEDIGGNVQAV 361
>At3g01360.1 68416.m00057 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 319
Score = 32.7 bits (71), Expect = 0.27
Identities = 27/96 (28%), Positives = 44/96 (45%), Gaps = 1/96 (1%)
Frame = +2
Query: 335 IDAPGHRDFIKNMITGTSQADCAV-LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 511
I GH D+ + T Q +C + L+V TG F +KNG R+ LG +
Sbjct: 226 IKCKGHGDYHRAKAIATLQFNCHLALMVVVATGLFSVIANKNGYLRQDHSKYRPLGAELE 285
Query: 512 IVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYN 619
+ +DS E +E+++E S+ K++G N
Sbjct: 286 NLSTFTLDSDEE-------DEVREE-SNVAKEVGLN 313
>At5g46180.1 68418.m05680 ornithine aminotransferase, putative /
ornithine--oxo-acid aminotransferase, putative similar
to SP|Q92413 Ornithine aminotransferase (EC 2.6.1.13)
(Ornithine--oxo-acid aminotransferase) [Aspergillus
nidulans] {Emericella nidulans}; contains Pfam profile
PF00202: aminotransferase, class III
Length = 475
Score = 30.3 bits (65), Expect = 1.4
Identities = 17/54 (31%), Positives = 28/54 (51%)
Frame = +2
Query: 491 TLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPXAVAFVPISG 652
T GV++ G+ + +S PP S R E++ E S++ Y+P V F +G
Sbjct: 18 TAGVRRSYGGLPQSNSKSPPSSSQRLMELESEFSAH----NYHPVPVVFSRANG 67
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 30.3 bits (65), Expect = 1.4
Identities = 21/68 (30%), Positives = 34/68 (50%)
Frame = +2
Query: 332 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 511
+ID PGH F G+S D A+L+V + + G+ QT E +L + +
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV-----DIKHGLEP--QTIE-SLNLLRMRNTEF 756
Query: 512 IVGVNKMD 535
I+ +NK+D
Sbjct: 757 IIALNKVD 764
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 30.3 bits (65), Expect = 1.4
Identities = 22/70 (31%), Positives = 33/70 (47%)
Frame = +2
Query: 326 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 505
+ +ID PGH F G++ D A+L+V + G+ QT E L VK
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV-----DIMRGLEP--QTIESLNLLRRRNVK 610
Query: 506 QLIVGVNKMD 535
I+ +NK+D
Sbjct: 611 -FIIALNKVD 619
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.9
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 332 IIDAPGHRDFIKNMITGTSQADCAVLIV 415
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.9
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +2
Query: 332 IIDAPGHRDFIKNMITGTSQADCAVLIV 415
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 29.5 bits (63), Expect = 2.5
Identities = 26/99 (26%), Positives = 38/99 (38%), Gaps = 4/99 (4%)
Frame = +2
Query: 251 KLKAERERGITIDIALWKFET----SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 418
K+ A GIT I +K +D PGH F G D A+++VA
Sbjct: 525 KVAASEAGGITQGIGAYKVSVPVDGKLQSCVFLDTPGHEAFGAMRARGARVTDIAIIVVA 584
Query: 419 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD 535
A G QT E A+ +++ +NK+D
Sbjct: 585 ADDG-------IRPQTNE-AIAHAKAAAVPIVIAINKID 615
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 5.8
Identities = 14/33 (42%), Positives = 20/33 (60%), Gaps = 3/33 (9%)
Frame = +2
Query: 554 SEPR---FEEIKKEVSSYIKKIGYNPXAVAFVP 643
SEP+ FEE K+V ++K+ GYN + VP
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVP 289
>At1g26130.1 68414.m03190 haloacid dehalogenase-like hydrolase
family protein similar to Potential
phospholipid-transporting ATPase (EC 3.6.3.1) from Homo
sapiens [SP|Q9Y2Q0, SP|O43520], Mus musculus [SP|P98200,
SP|P70704], {Bos taurus} SP|Q29449; contains InterPro
accession IPR005834: Haloacid dehalogenase-like
hydrolase
Length = 1184
Score = 27.9 bits (59), Expect = 7.6
Identities = 15/53 (28%), Positives = 24/53 (45%)
Frame = +2
Query: 437 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSS 595
E+G +TR+H G++ LI+ ++D E R E K VS+
Sbjct: 629 ESGRKYEKETRDHVNEYADAGLRTLILAYRELDENEYEVFTERISEAKNSVSA 681
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,585,421
Number of Sequences: 28952
Number of extensions: 343443
Number of successful extensions: 1064
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 1011
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1049
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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