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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0500
         (700 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g60600.1 68416.m06781 vesicle-associated membrane protein, pu...    33   0.14 
At3g17740.1 68416.m02264 expressed protein                             29   2.2  
At3g15470.1 68416.m01962 WD-40 repeat family protein contains Pf...    29   2.2  
At5g42010.1 68418.m05114 WD-40 repeat family protein contains Pf...    28   5.2  

>At3g60600.1 68416.m06781 vesicle-associated membrane protein,
           putative / VAMP, putative similar to VAP27 GI:6688926
           [Nicotiana plumbaginifolia]
          Length = 256

 Score = 33.5 bits (73), Expect = 0.14
 Identities = 22/64 (34%), Positives = 30/64 (46%), Gaps = 1/64 (1%)
 Frame = +1

Query: 343 KYTRQGTIASPIVVWRMKAPEKKPLIRPEKPEEVRAKVRRGSQKKQPSPIH-ALAEFQSP 519
           K+  QG IASP V  +   PE        + EE + +V   +  + PSP+H    E  SP
Sbjct: 102 KFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRVTYVAPPRPPSPVHEGSEEGSSP 161

Query: 520 ADSV 531
             SV
Sbjct: 162 RASV 165


>At3g17740.1 68416.m02264 expressed protein
          Length = 1149

 Score = 29.5 bits (63), Expect = 2.2
 Identities = 14/43 (32%), Positives = 24/43 (55%)
 Frame = -3

Query: 470  WLPRRTFALTSSGFSGLMRGFFSGAFMRQTTIGDAMVPWRVYL 342
            WL   ++ L+  G S  +RG F  A + Q T  ++++ WR Y+
Sbjct: 1035 WLFALSYELSKGGSSHRIRGLFERA-LAQDTQNNSVILWRCYI 1076


>At3g15470.1 68416.m01962 WD-40 repeat family protein contains Pfam
           PF00400: WD domain, G-beta repeat; similar to WD-repeat
           protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo
           sapiens]
          Length = 883

 Score = 29.5 bits (63), Expect = 2.2
 Identities = 25/92 (27%), Positives = 38/92 (41%), Gaps = 6/92 (6%)
 Frame = +1

Query: 157 LQRIALYCHQIQITSKVKADVQNISGELIVSGLDS------ATSLIQAAKNLMNAVVLTV 318
           LQ      HQ +IT      V   S E++V+  DS       T L+   K   N     +
Sbjct: 637 LQNKKKKAHQKKITGF--QFVPGSSSEVLVTSSDSRIRVVDGTDLVNKLKGFRNTSS-QI 693

Query: 319 KASYVASTKYTRQGTIASPIVVWRMKAPEKKP 414
            AS  A  KY    +  S + +W+ ++P  +P
Sbjct: 694 SASITADGKYVVSASEDSHVYIWKYESPASRP 725


>At5g42010.1 68418.m05114 WD-40 repeat family protein contains Pfam
           PF00400: WD domain, G-beta repeat; similar to WD-repeat
           protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo
           sapiens]
          Length = 709

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 19/70 (27%), Positives = 33/70 (47%)
 Frame = +1

Query: 241 IVSGLDSATSLIQAAKNLMNAVVLTVKASYVASTKYTRQGTIASPIVVWRMKAPEKKPLI 420
           I+ G+D+   L + A +L   ++    AS+ +  K+       S I VW    P KK   
Sbjct: 523 IICGVDTICKL-KKASSLRTTLMSPTFASFTSDGKHIVSTIEESGIHVWDFSQPNKK--A 579

Query: 421 RPEKPEEVRA 450
             +KP+ +R+
Sbjct: 580 SSQKPKTIRS 589


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,898,124
Number of Sequences: 28952
Number of extensions: 236140
Number of successful extensions: 890
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 851
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 887
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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