Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0499
(750 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g25130.1 68416.m03138 expressed protein 33 0.15
At2g01490.1 68415.m00072 phytanoyl-CoA dioxygenase (PhyH) family... 33 0.15
At5g64230.1 68418.m08068 expressed protein 32 0.47
At5g66540.1 68418.m08389 expressed protein ; supported by full-L... 31 0.82
At5g52590.1 68418.m06530 RabGAP/TBC domain-containing protein co... 31 0.82
At1g48540.2 68414.m05428 leucine-rich repeat family protein 31 1.1
At1g48540.1 68414.m05427 leucine-rich repeat family protein 31 1.1
At4g31270.1 68417.m04438 expressed protein 30 1.9
At3g56860.3 68416.m06325 UBP1 interacting protein 2a (UBA2a) ide... 30 1.9
At3g56860.2 68416.m06324 UBP1 interacting protein 2a (UBA2a) ide... 30 1.9
At3g56860.1 68416.m06323 UBP1 interacting protein 2a (UBA2a) ide... 30 1.9
At3g18850.2 68416.m02394 phospholipid/glycerol acyltransferase f... 29 2.5
At3g18850.1 68416.m02393 phospholipid/glycerol acyltransferase f... 29 2.5
At5g42710.1 68418.m05202 hypothetical protein 29 3.3
At5g39680.1 68418.m04805 pentatricopeptide (PPR) repeat-containi... 29 3.3
At4g31900.1 68417.m04533 chromatin remodeling factor, putative s... 29 3.3
At4g34100.1 68417.m04838 zinc finger (C3HC4-type RING finger) fa... 29 4.4
At4g07350.1 68417.m01129 hypothetical protein 29 4.4
At3g59180.1 68416.m06598 hypothetical protein contains a novel d... 28 5.8
At3g50130.1 68416.m05480 expressed protein ; expression supporte... 28 5.8
At1g62390.1 68414.m07039 octicosapeptide/Phox/Bem1p (PB1) domain... 28 5.8
At1g13450.1 68414.m01572 DNA binding protein GT-1 identical to D... 28 5.8
At5g24880.1 68418.m02946 expressed protein ; expression supporte... 28 7.6
At3g12775.1 68416.m01595 ubiquitin-conjugating enzyme family pro... 28 7.6
At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3) ... 28 7.6
At2g19120.1 68415.m02232 tRNA-splicing endonuclease positive eff... 28 7.6
>At3g25130.1 68416.m03138 expressed protein
Length = 406
Score = 33.5 bits (73), Expect = 0.15
Identities = 15/45 (33%), Positives = 27/45 (60%)
Frame = +2
Query: 212 VVYKKLNFLDDILVDSTEDMEAEESNQINEQKPKTEQEVITDNQE 346
V + L F D I+++ E+ + E ++ EQK K + +V+ DN+E
Sbjct: 175 VEIQPLKFEDVIVLEKEEETKKCEKEEVEEQKVKHKSDVVLDNRE 219
>At2g01490.1 68415.m00072 phytanoyl-CoA dioxygenase (PhyH) family
protein contains Pfam profile PF05721: Phytanoyl-CoA
dioxygenase (PhyH); weak similarity to Phytanoyl-CoA
dioxygenase, peroxisomal precursor (EC 1.14.11.18)
(Phytanoyl-CoA alpha-hydroxylase) (PhyH) (Phytanic acid
oxidase) (Swiss-Prot:O14832) [Homo sapiens]
Length = 283
Score = 33.5 bits (73), Expect = 0.15
Identities = 14/31 (45%), Positives = 18/31 (58%)
Frame = +1
Query: 472 ENGTDAKRHATSENITETTTCKWTKDRTIHR 564
EN + RHA S ++ E+ CKW KD I R
Sbjct: 242 ENLSSKSRHAYSLHVVESDGCKWAKDNWIQR 272
>At5g64230.1 68418.m08068 expressed protein
Length = 379
Score = 31.9 bits (69), Expect = 0.47
Identities = 20/63 (31%), Positives = 30/63 (47%), Gaps = 1/63 (1%)
Frame = +2
Query: 539 GPKIVQYIEDQNQIPVASRT-QTIDKLIERILKENTNEIDVFFXSMASSVKKLPPNLYLS 715
G I Q + N+IPV+SR Q + L ER++ +N E V + V LS
Sbjct: 99 GRAIWQLLSAMNEIPVSSRKYQVVRSLAERLINDNQGENSVALLDLNRRVLNASFRTTLS 158
Query: 716 QME 724
++E
Sbjct: 159 RLE 161
>At5g66540.1 68418.m08389 expressed protein ; supported by
full-Length cDNA gi:12057175 from [Arabidopsis thaliana]
Length = 524
Score = 31.1 bits (67), Expect = 0.82
Identities = 20/64 (31%), Positives = 36/64 (56%), Gaps = 1/64 (1%)
Frame = +2
Query: 233 FLDDILVDSTEDMEAEESN-QINEQKPKTEQEVITDNQEYYEIRLVDNADIESKFIKILP 409
F D + D +++E+ +S + E++ + E+E + +E E + DN IE KF KI
Sbjct: 119 FDSDDVDDEDKEIESNDSEGEDEEEEEEDEEEEEEEEEEEEEEKDGDNEGIEDKFFKI-- 176
Query: 410 KQIE 421
K++E
Sbjct: 177 KELE 180
>At5g52590.1 68418.m06530 RabGAP/TBC domain-containing protein
contains similarity to SP|P48365 GTPase-activating
protein GYP7 {Saccharomyces cerevisiae}; contains Pfam
profile PF00566: TBC domain
Length = 338
Score = 31.1 bits (67), Expect = 0.82
Identities = 17/38 (44%), Positives = 21/38 (55%), Gaps = 1/38 (2%)
Frame = -3
Query: 658 HINFI-GILLKNSFYEFIDGLCSGCDWYLILIFYVLYD 548
H+N + ILL SFY F G C G YL I +V+ D
Sbjct: 125 HVNSMRDILLTYSFYNFDLGYCQGMSDYLSPILFVMED 162
>At1g48540.2 68414.m05428 leucine-rich repeat family protein
Length = 1051
Score = 30.7 bits (66), Expect = 1.1
Identities = 22/94 (23%), Positives = 40/94 (42%), Gaps = 3/94 (3%)
Frame = +2
Query: 167 TRSKRLGSMPGYTKLVVYKKLNFLDDILVDSTEDMEAEESNQINEQKPKTEQEVITDNQE 346
T S GS P Y K V+Y++ N +++IL S + S+ + + ++
Sbjct: 580 TGSTYPGSPPHYQKDVLYRRHNLVEEILQLSADSYSVASSDSTSSCSEDDNYDSESEYSN 639
Query: 347 YYEIRLVDNADIE---SKFIKILPKQIENSNKQP 439
+ E RL D ++ + ++ PK QP
Sbjct: 640 HKEGRLTDLLNVNRLGKEILECTPKGTRFLGSQP 673
>At1g48540.1 68414.m05427 leucine-rich repeat family protein
Length = 1063
Score = 30.7 bits (66), Expect = 1.1
Identities = 22/94 (23%), Positives = 40/94 (42%), Gaps = 3/94 (3%)
Frame = +2
Query: 167 TRSKRLGSMPGYTKLVVYKKLNFLDDILVDSTEDMEAEESNQINEQKPKTEQEVITDNQE 346
T S GS P Y K V+Y++ N +++IL S + S+ + + ++
Sbjct: 580 TGSTYPGSPPHYQKDVLYRRHNLVEEILQLSADSYSVASSDSTSSCSEDDNYDSESEYSN 639
Query: 347 YYEIRLVDNADIE---SKFIKILPKQIENSNKQP 439
+ E RL D ++ + ++ PK QP
Sbjct: 640 HKEGRLTDLLNVNRLGKEILECTPKGTRFLGSQP 673
>At4g31270.1 68417.m04438 expressed protein
Length = 294
Score = 29.9 bits (64), Expect = 1.9
Identities = 24/93 (25%), Positives = 43/93 (46%), Gaps = 7/93 (7%)
Frame = +2
Query: 101 AKQISRPVEECRKRWRGLRDSYTRSKR-----LGSMPGYTKLVVYKK--LNFLDDILVDS 259
A +SR + +CR++W L Y + K+ G+ Y L K+ LN DI ++
Sbjct: 57 ALDVSRNLNQCRRKWDSLMSDYNQIKKWESQYRGTGRSYWSLSSDKRKLLNLPGDIDIEL 116
Query: 260 TEDMEAEESNQINEQKPKTEQEVITDNQEYYEI 358
E + A I ++K TE + + Q+ ++
Sbjct: 117 FEAINAVV--MIQDEKAGTESDSDPEAQDVVDL 147
>At3g56860.3 68416.m06325 UBP1 interacting protein 2a (UBA2a)
identical to UBP1 interacting protein 2a [Arabidopsis
thaliana] GI:19682816; contains InterPro entry
IPR000504: RNA-binding region RNP-1 (RNA recognition
motif) (RRM)
Length = 478
Score = 29.9 bits (64), Expect = 1.9
Identities = 14/48 (29%), Positives = 29/48 (60%), Gaps = 1/48 (2%)
Frame = +2
Query: 251 VDSTEDMEAEESNQINEQKPKTEQEVITDNQEYY-EIRLVDNADIESK 391
++ E EAEE +Q +Q P+ EQ+++ NQ+ ++ V+ ++E +
Sbjct: 7 LEGEESNEAEEPSQKLKQTPEEEQQLVIKNQDNQGDVEEVEYEEVEEE 54
>At3g56860.2 68416.m06324 UBP1 interacting protein 2a (UBA2a)
identical to UBP1 interacting protein 2a [Arabidopsis
thaliana] GI:19682816; contains InterPro entry
IPR000504: RNA-binding region RNP-1 (RNA recognition
motif) (RRM)
Length = 478
Score = 29.9 bits (64), Expect = 1.9
Identities = 14/48 (29%), Positives = 29/48 (60%), Gaps = 1/48 (2%)
Frame = +2
Query: 251 VDSTEDMEAEESNQINEQKPKTEQEVITDNQEYY-EIRLVDNADIESK 391
++ E EAEE +Q +Q P+ EQ+++ NQ+ ++ V+ ++E +
Sbjct: 7 LEGEESNEAEEPSQKLKQTPEEEQQLVIKNQDNQGDVEEVEYEEVEEE 54
>At3g56860.1 68416.m06323 UBP1 interacting protein 2a (UBA2a)
identical to UBP1 interacting protein 2a [Arabidopsis
thaliana] GI:19682816; contains InterPro entry
IPR000504: RNA-binding region RNP-1 (RNA recognition
motif) (RRM)
Length = 478
Score = 29.9 bits (64), Expect = 1.9
Identities = 14/48 (29%), Positives = 29/48 (60%), Gaps = 1/48 (2%)
Frame = +2
Query: 251 VDSTEDMEAEESNQINEQKPKTEQEVITDNQEYY-EIRLVDNADIESK 391
++ E EAEE +Q +Q P+ EQ+++ NQ+ ++ V+ ++E +
Sbjct: 7 LEGEESNEAEEPSQKLKQTPEEEQQLVIKNQDNQGDVEEVEYEEVEEE 54
>At3g18850.2 68416.m02394 phospholipid/glycerol acyltransferase
family protein contains Pfam profile: PF01553
Acyltransferase
Length = 375
Score = 29.5 bits (63), Expect = 2.5
Identities = 20/60 (33%), Positives = 30/60 (50%), Gaps = 9/60 (15%)
Frame = -2
Query: 566 LLCIVRSLVHLQVVVSVIF---------SLVACLLASVPFSYDHCSFFLFSWVVCYCFLF 414
L+ ++R ++ L V+VS F S V L S+ +S SFF SW+ + FLF
Sbjct: 14 LIRVLRGIICLMVLVSTAFMMLIFWGFLSAVVLRLFSIRYSRKCVSFFFGSWLALWPFLF 73
>At3g18850.1 68416.m02393 phospholipid/glycerol acyltransferase
family protein contains Pfam profile: PF01553
Acyltransferase
Length = 375
Score = 29.5 bits (63), Expect = 2.5
Identities = 20/60 (33%), Positives = 30/60 (50%), Gaps = 9/60 (15%)
Frame = -2
Query: 566 LLCIVRSLVHLQVVVSVIF---------SLVACLLASVPFSYDHCSFFLFSWVVCYCFLF 414
L+ ++R ++ L V+VS F S V L S+ +S SFF SW+ + FLF
Sbjct: 14 LIRVLRGIICLMVLVSTAFMMLIFWGFLSAVVLRLFSIRYSRKCVSFFFGSWLALWPFLF 73
>At5g42710.1 68418.m05202 hypothetical protein
Length = 807
Score = 29.1 bits (62), Expect = 3.3
Identities = 22/73 (30%), Positives = 33/73 (45%), Gaps = 2/73 (2%)
Frame = +2
Query: 221 KKLNFLDDILVDSTEDMEAEESNQIN--EQKPKTEQEVITDNQEYYEIRLVDNADIESKF 394
+ L L DI S N+I E+ ++E NQ Y +I+ V ADIE +
Sbjct: 195 QSLRLLVDIQQASEYTSHKRRKNRIKLLEENGDDDEEEDAHNQNYQKIKQVAKADIEMRL 254
Query: 395 IKILPKQIENSNK 433
+ L Q + +NK
Sbjct: 255 L-ALNYQEDKNNK 266
>At5g39680.1 68418.m04805 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 710
Score = 29.1 bits (62), Expect = 3.3
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = -3
Query: 649 FIGILLKNSFYEFIDGLCSGCDWY 578
+I I N F+ F+DG CS CD++
Sbjct: 687 YIVIRDSNRFHHFLDGQCSCCDYW 710
>At4g31900.1 68417.m04533 chromatin remodeling factor, putative
strong similarity to chromatin remodeling factor CHD3
(PICKLE) [Arabidopsis thaliana] GI:6478518; contains
Pfam profiles PF00271: Helicase conserved C-terminal
domain, PF00176: SNF2 family N-terminal domain
Length = 1202
Score = 29.1 bits (62), Expect = 3.3
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +2
Query: 236 LDDILVDSTEDMEAEESNQINEQKPKT 316
LDD+ V S E+ EA+E ++KP+T
Sbjct: 832 LDDLKVTSDEEEEADEPEAARQRKPRT 858
>At4g34100.1 68417.m04838 zinc finger (C3HC4-type RING finger) family
protein contains Pfam profile: PF00097 zinc finger, C3HC4
type (RING finger)
Length = 1092
Score = 28.7 bits (61), Expect = 4.4
Identities = 14/62 (22%), Positives = 27/62 (43%)
Frame = -2
Query: 593 WMRLVFDSDLLCIVRSLVHLQVVVSVIFSLVACLLASVPFSYDHCSFFLFSWVVCYCFLF 414
W +VF S +L + + + V++ ++F L+ + VP W + FL
Sbjct: 866 WCGIVFKSSVLLAIWVFI-IPVLIGLLFELLVIVPMRVPVDESPVFLLYQDWALGLIFLK 924
Query: 413 VW 408
+W
Sbjct: 925 IW 926
>At4g07350.1 68417.m01129 hypothetical protein
Length = 330
Score = 28.7 bits (61), Expect = 4.4
Identities = 11/40 (27%), Positives = 30/40 (75%)
Frame = +2
Query: 239 DDILVDSTEDMEAEESNQINEQKPKTEQEVITDNQEYYEI 358
DD++++ST+D+E ++ + E+K + E+++ + +E++E+
Sbjct: 285 DDVIIESTKDLEFDQ-ELVAEEKLEAEEQL--EPKEHFEV 321
>At3g59180.1 68416.m06598 hypothetical protein contains a novel
domain with similarity to F-box domain;
Length = 475
Score = 28.3 bits (60), Expect = 5.8
Identities = 18/56 (32%), Positives = 29/56 (51%)
Frame = +2
Query: 182 LGSMPGYTKLVVYKKLNFLDDILVDSTEDMEAEESNQINEQKPKTEQEVITDNQEY 349
L +MP ++++Y F DD+ + STE E+ PK + +VI+DN Y
Sbjct: 403 LETMPNLEQVILYYDTPFDDDLSILSTELQMLEK-----VASPKCKIQVISDNISY 453
>At3g50130.1 68416.m05480 expressed protein ; expression supported
by MPSS
Length = 564
Score = 28.3 bits (60), Expect = 5.8
Identities = 15/52 (28%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
Frame = +2
Query: 92 QEIAKQISRPVEECRKRWR-GLRDSYTRSKRLGSMPGYTKLVVYKKLNFLDD 244
Q++ KQ +P EE R+ W +RD ++ R + + KL +Y+ +L +
Sbjct: 100 QKLQKQNQKP-EETREEWVISIRDKMEQALREDATTSWDKLCIYRVPQYLQE 150
>At1g62390.1 68414.m07039 octicosapeptide/Phox/Bem1p (PB1)
domain-containing protein / tetratricopeptide repeat
(TPR)-containing protein contains Pfam profiles PF00564:
PB1 domain, PF00515: TPR Domain
Length = 751
Score = 28.3 bits (60), Expect = 5.8
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = +2
Query: 122 VEECRKRWRGLRDSYTRSKRLGSMP 196
V+E R RWR L+ Y RLG MP
Sbjct: 283 VQEKRIRWRPLKFVYDHDIRLGQMP 307
>At1g13450.1 68414.m01572 DNA binding protein GT-1 identical to DNA
binding protein GT-1 GI:598073 from [Arabidopsis
thaliana]
Length = 406
Score = 28.3 bits (60), Expect = 5.8
Identities = 20/85 (23%), Positives = 39/85 (45%), Gaps = 5/85 (5%)
Frame = +2
Query: 89 WQEIA-----KQISRPVEECRKRWRGLRDSYTRSKRLGSMPGYTKLVVYKKLNFLDDILV 253
W++I+ K R C +WR L + ++K G K+ YK+ ++DIL
Sbjct: 116 WEQISSKMREKGFDRSPTMCTDKWRNLLKEFKKAKHHDRGNGSAKMSYYKE---IEDILR 172
Query: 254 DSTEDMEAEESNQINEQKPKTEQEV 328
+ ++ + + N+ + P T +V
Sbjct: 173 ERSKKVTPPQYNK-SPNTPPTSAKV 196
>At5g24880.1 68418.m02946 expressed protein ; expression supported
by MPSS
Length = 443
Score = 27.9 bits (59), Expect = 7.6
Identities = 14/56 (25%), Positives = 32/56 (57%)
Frame = +2
Query: 221 KKLNFLDDILVDSTEDMEAEESNQINEQKPKTEQEVITDNQEYYEIRLVDNADIES 388
+KL +D + + TE+M+ +++NQ N+ + + + + D E E ++ ++ES
Sbjct: 272 EKLIKNEDDIEEKTEEMKEQDNNQANKSEEEEDVKKKIDENETPEKVDTESKEVES 327
>At3g12775.1 68416.m01595 ubiquitin-conjugating enzyme family
protein contains Pfam profile PF00179:
Ubiquitin-conjugating enzyme
Length = 362
Score = 27.9 bits (59), Expect = 7.6
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = +2
Query: 239 DDILVDSTEDMEAEESNQINEQKPKTEQEVITD 337
DD STE E E N + E KP +E E + D
Sbjct: 28 DDRAGSSTEQEEDESQNLVPESKPGSEGEKVGD 60
>At2g23150.1 68415.m02765 NRAMP metal ion transporter 3 (NRAMP3)
identical to metal transporter Nramp3 [Arabidopsis
thaliana] gi|6468012|gb|AAF13278; member of the natural
resistance-associated macrophage protein (NRAMP) metal
transporter family, PMID:11500563
Length = 509
Score = 27.9 bits (59), Expect = 7.6
Identities = 16/40 (40%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = -3
Query: 481 YRFLTTTVLF-SYFLGLFVTVFYLFG*YFDELALNICIVN 365
Y +T LF S+ + LFVT + G Y +LA +I +VN
Sbjct: 279 YTIESTIALFISFLINLFVTTVFAKGFYNTDLANSIGLVN 318
>At2g19120.1 68415.m02232 tRNA-splicing endonuclease positive
effector-related similar to Endonuclease sen1
(Swiss-Prot:Q92355) [Schizosaccharomyces pombe]; similar
to tRNA-splicing endonuclease positive effector
(Swiss-Prot:Q00416) [Saccharomyces cerevisiae]
Length = 1090
Score = 27.9 bits (59), Expect = 7.6
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +2
Query: 551 VQYIEDQNQIPVASRTQTIDKLIERILKENTNE 649
V Y + + P S TQ D +ER+++E TNE
Sbjct: 211 VSYSQRSFKKPATSSTQYQDTSVERLIREVTNE 243
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,031,758
Number of Sequences: 28952
Number of extensions: 297329
Number of successful extensions: 1081
Number of sequences better than 10.0: 26
Number of HSP's better than 10.0 without gapping: 1034
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1078
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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