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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0485
         (650 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g36020.1 68417.m05128 cold-shock DNA-binding family protein c...    59   2e-09
At2g21060.1 68415.m02500 cold-shock DNA-binding family protein /...    54   1e-07
At4g38680.1 68417.m05477 cold-shock DNA-binding family protein c...    53   1e-07
At2g17870.1 68415.m02070 cold-shock DNA-binding family protein c...    51   6e-07
At5g50800.1 68418.m06293 nodulin MtN3 family protein similar to ...    27   8.2  

>At4g36020.1 68417.m05128 cold-shock DNA-binding family protein
           contains Pfam domains, PF00313: 'Cold-shock' DNA-binding
           domain and PF00098: Zinc knuckle
          Length = 299

 Score = 59.3 bits (137), Expect = 2e-09
 Identities = 31/74 (41%), Positives = 43/74 (58%), Gaps = 1/74 (1%)
 Frame = +3

Query: 141 AEKVSGTVKWFNVKSGYGFINRNDTKEDVFVHQTAIARNNPRKAVRSVGDGEAVEFAVVA 320
           A + +G V WFN   GYGFI  +D   ++FVHQ++I      +  RS+  G+AVEFA+  
Sbjct: 8   AARSTGKVNWFNASKGYGFITPDDGSVELFVHQSSIV----SEGYRSLTVGDAVEFAITQ 63

Query: 321 GEKG-FEAAGVTGP 359
           G  G  +A  VT P
Sbjct: 64  GSDGKTKAVNVTAP 77


>At2g21060.1 68415.m02500 cold-shock DNA-binding family protein /
           glycine-rich protein (GRP2) identical to Glycine-rich
           protein 2b (AtGRP2b) [Arabidopsis thaliana]
           SWISS-PROT:Q38896; contains Pfam domains PF00313:
           'Cold-shock' DNA-binding domain and PF00098: Zinc
           knuckle
          Length = 201

 Score = 53.6 bits (123), Expect = 1e-07
 Identities = 29/73 (39%), Positives = 44/73 (60%), Gaps = 1/73 (1%)
 Frame = +3

Query: 144 EKVSGTVKWFNVKSGYGFINRNDTKEDVFVHQTAIARNNPRKAVRSVGDGEAVEFAVVAG 323
           ++  GTVKWF+ + G+GFI  +D  +D+FVHQ++I      +  RS+   E+VEF V   
Sbjct: 13  DRRKGTVKWFDTQKGFGFITPSDGGDDLFVHQSSIR----SEGFRSLAAEESVEFDVEVD 68

Query: 324 EKGF-EAAGVTGP 359
             G  +A  V+GP
Sbjct: 69  NSGRPKAIEVSGP 81


>At4g38680.1 68417.m05477 cold-shock DNA-binding family protein
           contains Pfam domains PF00313: 'Cold-shock' DNA-binding
           domain and PF00098: Zinc knuckle
          Length = 203

 Score = 53.2 bits (122), Expect = 1e-07
 Identities = 29/73 (39%), Positives = 44/73 (60%), Gaps = 1/73 (1%)
 Frame = +3

Query: 144 EKVSGTVKWFNVKSGYGFINRNDTKEDVFVHQTAIARNNPRKAVRSVGDGEAVEFAV-VA 320
           E+  G+VKWF+ + G+GFI  +D  +D+FVHQ++I      +  RS+   EAVEF V + 
Sbjct: 9   ERRKGSVKWFDTQKGFGFITPDDGGDDLFVHQSSIR----SEGFRSLAAEEAVEFEVEID 64

Query: 321 GEKGFEAAGVTGP 359
                +A  V+GP
Sbjct: 65  NNNRPKAIDVSGP 77


>At2g17870.1 68415.m02070 cold-shock DNA-binding family protein
           contains Pfam domains, PF00313: 'Cold-shock' DNA-binding
           domain and PF00098: Zinc knuckle
          Length = 301

 Score = 51.2 bits (117), Expect = 6e-07
 Identities = 29/74 (39%), Positives = 42/74 (56%), Gaps = 1/74 (1%)
 Frame = +3

Query: 141 AEKVSGTVKWFNVKSGYGFINRNDTKEDVFVHQTAIARNNPRKAVRSVGDGEAVEFAVVA 320
           A +  G V WF+   GYGFI  +D  E++FVHQ++I  +      RS+  GE+VE+ +  
Sbjct: 8   AARSIGKVSWFSDGKGYGFITPDDGGEELFVHQSSIVSD----GFRSLTLGESVEYEIAL 63

Query: 321 GEKG-FEAAGVTGP 359
           G  G  +A  VT P
Sbjct: 64  GSDGKTKAIEVTAP 77


>At5g50800.1 68418.m06293 nodulin MtN3 family protein similar to
           MtN3 GI:1619602 (root nodule development) from [Medicago
           truncatula]
          Length = 294

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 11/24 (45%), Positives = 17/24 (70%)
 Frame = +1

Query: 247 SPVTTHVRLCARSATERRWSLPWL 318
           +PV T VR+C + +TE   SLP++
Sbjct: 26  APVPTFVRICKKKSTEGFQSLPYV 49


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,245,004
Number of Sequences: 28952
Number of extensions: 184395
Number of successful extensions: 574
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 566
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 573
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1354097952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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