Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0479
         (700 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g06000.1 68418.m00665 eukaryotic translation initiation facto...    79   4e-15
At3g11400.1 68416.m01390 eukaryotic translation initiation facto...    71   6e-13
At1g43560.1 68414.m05000 thioredoxin family protein contains Pfa...    29   3.0  
At3g03630.1 68416.m00366 cysteine synthase, chloroplast, putativ...    29   3.9  
At1g52650.1 68414.m05945 F-box family protein contains F-box dom...    28   6.8  
At5g57170.1 68418.m07141 macrophage migration inhibitory factor ...    27   9.0  
At2g36350.1 68415.m04461 protein kinase, putative similar to pro...    27   9.0  
At1g76760.1 68414.m08933 thioredoxin family protein similar to t...    27   9.0  

>At5g06000.1 68418.m00665 eukaryotic translation initiation factor
           3G, putative / eIF3g, putative similar to eukaryotic
           translation initiation factor 3g [Arabidopsis thaliana]
           GI:12407751; contains InterPro entry IPR000504:
           RNA-binding region RNP-1 (RNA recognition motif) (RRM)
          Length = 276

 Score = 78.6 bits (185), Expect = 4e-15
 Identities = 43/119 (36%), Positives = 70/119 (58%), Gaps = 5/119 (4%)
 Frame = +2

Query: 179 ENGLKIVTEYKYDNDNKKVKIVRTYKIEKRVVSKSIAKRKTWSKFGDSASDKPGPNPATT 358
           +NG+K V EYK++ ++KKVKI  T +++KR ++K   +R++W+KFGD+A ++   +  T 
Sbjct: 6   QNGVKKVIEYKFNEEDKKVKITTTTRVQKRALTKQAVERRSWNKFGDAAHEE-SSSYLTM 64

Query: 359 NVAEDVFMQFITS--KEXSQRPDDGE-LDGLKPPSSNVIFKCRTCQ--GDHLTLYCPFK 520
              ED+ ++ I +      Q    G+ +  L  P + V+  CR CQ  GDH T  CP K
Sbjct: 65  RSTEDIILERIRAPGSNAEQSTVSGDSMSQLGKPGA-VLMVCRLCQKKGDHWTSRCPQK 122


>At3g11400.1 68416.m01390 eukaryotic translation initiation factor
           3G / eIF3g nearly identical to eukaryotic translation
           initiation factor 3g [Arabidopsis thaliana] GI:12407751
          Length = 294

 Score = 71.3 bits (167), Expect = 6e-13
 Identities = 62/215 (28%), Positives = 96/215 (44%), Gaps = 21/215 (9%)
 Frame = +2

Query: 107 EFQASWADEVEIDQGVLPPPSEVV---ENGLKIVTEYKYDNDNKKVKI---VRTYKIEKR 268
           +F+    DE E D   L PP +V+   ENGLK   EYK++++  KVKI    R  K+   
Sbjct: 11  KFRWGEMDEDE-DLDFLLPPKQVIGPDENGLKTTIEYKFNDEENKVKITTRTRVRKLASA 69

Query: 269 VVSKSIAKRKTWSKFGDSASDKPGPNPATTNVAEDVFMQFITSKEXSQRPDDGELDGLKP 448
            ++K   +R+ W KFGD+A+++ G +  T    E++ ++   +            DGL  
Sbjct: 70  RLNKRAMERRNWPKFGDAANEEAGSH-LTMVSTEEILLERPRAPGTKADESKATGDGLSQ 128

Query: 449 --PSSNVIFKCRTC--QGDHLTLYCPFKH----TQIXXXXXXXXXXXXXXXXXXSNK--Y 598
                 V+  CR C  +GDH T  CP+K     T +                  + K  Y
Sbjct: 129 LGKGGAVLMVCRICHKKGDHWTSKCPYKDLAAPTDVFIDKPPTGESSTMSAAPGTGKAAY 188

Query: 599 IPPSSAGRIPGRDQPPV-----RREXVTAIRISNL 688
           +PPS      G D+  V     RR    ++R++NL
Sbjct: 189 VPPSMRA---GADRSAVGSDMRRRNDENSVRVTNL 220


>At1g43560.1 68414.m05000 thioredoxin family protein contains Pfam
           profile: PF00085 Thioredoxin; similar to thioredoxin
           GI:142153 from [Synechococcus PCC6301]
          Length = 167

 Score = 29.1 bits (62), Expect = 3.0
 Identities = 20/75 (26%), Positives = 32/75 (42%)
 Frame = +2

Query: 89  DMPVAEEFQASWADEVEIDQGVLPPPSEVVENGLKIVTEYKYDNDNKKVKIVRTYKIEKR 268
           D PV  +F A+W    ++   ++P  +EV E    I+   K D + K   +   Y+IE  
Sbjct: 76  DKPVLVDFYATWCGPCQL---MVPILNEVSETLKDIIAVVKIDTE-KYPSLANKYQIEAL 131

Query: 269 VVSKSIAKRKTWSKF 313
                    K W +F
Sbjct: 132 PTFILFKDGKLWDRF 146


>At3g03630.1 68416.m00366 cysteine synthase, chloroplast, putative /
           O-acetylserine (thiol)-lyase, putative / O-acetylserine
           sulfhydrylase, putative identical to SP|O22682 Probable
           cysteine synthase, chloroplast precursor {Arabidopsis
           thaliana}, similar to SP|P31300 Cysteine synthase,
           chloroplast precursor {Capsicum annuum}
          Length = 404

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 17/56 (30%), Positives = 24/56 (42%)
 Frame = +2

Query: 206 YKYDNDNKKVKIVRTYKIEKRVVSKSIAKRKTWSKFGDSASDKPGPNPATTNVAED 373
           + + +D+     VRT  +   VV     K  T +K       KP P P  T VAE+
Sbjct: 34  FSFHHDSSSSLAVRT-PVSSFVVGAISGKSSTGTKSKSKTKRKPPPPPPVTTVAEE 88


>At1g52650.1 68414.m05945 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 465

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 12/31 (38%), Positives = 20/31 (64%)
 Frame = -3

Query: 194 FLVHFQPLQKVVEVHLGQSRPHQPRKPEILQ 102
           FL HF P  + +++H+ ++ P Q R PE+ Q
Sbjct: 404 FLEHF-PCLREIKIHMEENGPTQLRVPEVSQ 433


>At5g57170.1 68418.m07141 macrophage migration inhibitory factor
           family protein / MIF family protein contains Pfam
           profile: PF01187 Macrophage migration inhibitory
           factor(MIF)
          Length = 115

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 10/35 (28%), Positives = 23/35 (65%)
 Frame = -2

Query: 354 VAGLGPGLSLAESPNLLQVLRLAILFETTRFSILY 250
           + GLGPG++   S  + ++L++ +  +++RF I +
Sbjct: 66  IGGLGPGVNGKLSETISEILQIKLSIDSSRFYIKF 100


>At2g36350.1 68415.m04461 protein kinase, putative similar to
           protein kinase KIPK (KCBP-interacting protein kinase)
           [Arabidopsis thaliana] gi|7716430|gb|AAF68383
          Length = 949

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 15/54 (27%), Positives = 28/54 (51%)
 Frame = +2

Query: 131 EVEIDQGVLPPPSEVVENGLKIVTEYKYDNDNKKVKIVRTYKIEKRVVSKSIAK 292
           E+EID+    PP  V+E+ L  + ++     +     V T +++K +VS +  K
Sbjct: 266 EIEIDENHTSPPHMVIEDQLVEIDKHVTSLPSCSGSKVDTEELDKSIVSSARVK 319


>At1g76760.1 68414.m08933 thioredoxin family protein similar to
           thioredoxin CH2, M-type, chloroplast precursor GB:P23400
           SP|P23400 [Chlamydomonas reinhardtii]; contains Pfam
           profile: PF00085 Thioredoxin
          Length = 172

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 17/58 (29%), Positives = 29/58 (50%)
 Frame = +2

Query: 89  DMPVAEEFQASWADEVEIDQGVLPPPSEVVENGLKIVTEYKYDNDNKKVKIVRTYKIE 262
           D PV  ++ A+W    +    +L   SE +++ +++V   K D + K   I   YKIE
Sbjct: 81  DKPVLVDYYATWCGPCQFMVPILNEVSETLKDKIQVV---KIDTE-KYPSIANKYKIE 134


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,064,527
Number of Sequences: 28952
Number of extensions: 234714
Number of successful extensions: 663
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 648
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 659
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)