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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0476
         (750 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)             248   3e-66
SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)                     244   6e-65
SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)             162   3e-40
SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44)            141   6e-34
SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.)              83   2e-16
SB_23775| Best HMM Match : Tropomyosin (HMM E-Value=0)                 32   0.43 
SB_33101| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   2.3  
SB_11347| Best HMM Match : No HMM Matches (HMM E-Value=.)              29   4.0  
SB_47927| Best HMM Match : TSP_1 (HMM E-Value=9.5e-34)                 28   7.0  
SB_15350| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   7.0  
SB_12646| Best HMM Match : SSrecog (HMM E-Value=0)                     28   7.0  
SB_10602| Best HMM Match : RVT_1 (HMM E-Value=1.3e-10)                 28   9.3  

>SB_34219| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 309

 Score =  248 bits (608), Expect = 3e-66
 Identities = 126/193 (65%), Positives = 143/193 (74%), Gaps = 2/193 (1%)
 Frame = +3

Query: 177 MAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKE 356
           MA AMKE TE    L  EERNLLSVAYKNVVGA+RSSWRVISSIEQK EGSERK+Q  + 
Sbjct: 1   MAKAMKEATEISETLEQEERNLLSVAYKNVVGAKRSSWRVISSIEQKLEGSERKKQNTET 60

Query: 357 YRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSV 536
           YR  +E EL E+C  VL LL+  LIP A + ESKVFYLKMKGDYYRY  EVA  + R  V
Sbjct: 61  YRQTIENELNEVCETVLKLLESKLIPNAQSTESKVFYLKMKGDYYRYEGEVAGADRRREV 120

Query: 537 VEDSQKAYQDAFEIS--XAKMQPTHPIXLGLALNFSXFYYEILNSPDKACQLAKQAFDDA 710
           V+ + KAY +A EI+    K+ PT PI LGLALNFS FYYEI+    +AC LAK+AFDDA
Sbjct: 121 VQKAMKAYSEAQEIAEKDPKLPPTDPIRLGLALNFSVFYYEIVEDSKQACDLAKKAFDDA 180

Query: 711 IAELDTLNEXSYK 749
           IAELDTL+E  YK
Sbjct: 181 IAELDTLSEDQYK 193



 Score = 90.2 bits (214), Expect = 2e-18
 Identities = 45/76 (59%), Positives = 58/76 (76%), Gaps = 3/76 (3%)
 Frame = +3

Query: 531 SVVEDSQKAYQDAFEISXA---KMQPTHPIXLGLALNFSXFYYEILNSPDKACQLAKQAF 701
           +VVE + +AY++A E +     K+ PT PI LGLALNFS F+YEI  + ++AC+LAKQAF
Sbjct: 207 TVVEKALQAYKEAKEAAETGDGKLAPTDPIRLGLALNFSVFHYEIQENQEEACKLAKQAF 266

Query: 702 DDAIAELDTLNEXSYK 749
           DDAIAELD+LNE  YK
Sbjct: 267 DDAIAELDSLNEDQYK 282


>SB_36368| Best HMM Match : 14-3-3 (HMM E-Value=0)
          Length = 248

 Score =  244 bits (597), Expect = 6e-65
 Identities = 124/214 (57%), Positives = 155/214 (72%), Gaps = 3/214 (1%)
 Frame = +3

Query: 117 TRXELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRV 296
           +R EL+  AK+AEQAERYDDM  AM  VT+ G  L++EERNLLSVAYKNVVGARRSSWRV
Sbjct: 10  SREELIHLAKMAEQAERYDDMVNAMSAVTKEGKPLNDEERNLLSVAYKNVVGARRSSWRV 69

Query: 297 ISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKAS---NPESKVFY 467
           ISS+EQK    E    + K+YR  +  EL   C +VL +L+ +L+       N E+KVFY
Sbjct: 70  ISSMEQK--APEEMAALTKKYREDITNELNGKCAEVLDILENYLLKDGQDDINTEAKVFY 127

Query: 468 LKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISXAKMQPTHPIXLGLALNFSXFY 647
           LKM+GDY+RYL EVA G++R   +E S++AY+DA      ++ P+HPI LGLALNFS FY
Sbjct: 128 LKMRGDYHRYLVEVAEGDSRKENIEKSREAYKDA-SAKAEELSPSHPIRLGLALNFSVFY 186

Query: 648 YEILNSPDKACQLAKQAFDDAIAELDTLNEXSYK 749
           YEI N P +AC+LAK+AFDDAIA LD L + SYK
Sbjct: 187 YEIENKPPEACKLAKEAFDDAIAVLDNLKDESYK 220


>SB_34217| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 219

 Score =  162 bits (393), Expect = 3e-40
 Identities = 79/134 (58%), Positives = 104/134 (77%)
 Frame = +3

Query: 120 RXELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVI 299
           R  L+  AKL+EQ +RYD+MA  MKEV+E   +LS EERNLLSV+YKN+VG RRSSWRVI
Sbjct: 84  RETLIYNAKLSEQCDRYDEMAKIMKEVSEKYPKLSKEERNLLSVSYKNIVGQRRSSWRVI 143

Query: 300 SSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYLKMK 479
           SSIE+KT  S     + K+Y+  +EKEL+++C +VLG+L++ LIP A + E+KVFY K+K
Sbjct: 144 SSIEEKTAESS-SLAIVKKYKACIEKELKDLCKEVLGILER-LIPGAEDEENKVFYFKLK 201

Query: 480 GDYYRYLAEVATGE 521
           GDYYRYLAE + G+
Sbjct: 202 GDYYRYLAEFSHGQ 215


>SB_37955| Best HMM Match : 14-3-3 (HMM E-Value=6.5861e-44)
          Length = 251

 Score =  141 bits (341), Expect = 6e-34
 Identities = 72/122 (59%), Positives = 91/122 (74%), Gaps = 3/122 (2%)
 Frame = +3

Query: 120 RXELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVI 299
           + E V  AKLAEQAERYDDM  +MKEV + G ELS E+RNLLSVAYKNV+GARR+SWR+I
Sbjct: 4   KEEHVYMAKLAEQAERYDDMVNSMKEVAKMGTELSTEDRNLLSVAYKNVIGARRASWRII 63

Query: 300 SSIEQKTE--GSE-RKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVFYL 470
           +SIEQK E  G +  K +M + YR  +E+EL+ IC ++L LLD  LI  + + ESKVFY 
Sbjct: 64  TSIEQKEESKGEDMAKLEMIRNYRKTIEEELKTICGEILSLLDDSLIKNSQSEESKVFYN 123

Query: 471 KM 476
           K+
Sbjct: 124 KI 125


>SB_34218| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 607

 Score = 83.4 bits (197), Expect = 2e-16
 Identities = 43/99 (43%), Positives = 63/99 (63%), Gaps = 2/99 (2%)
 Frame = +3

Query: 117 TRXELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRV 296
           +R ELVQ AKLAEQ ER++D+   MK+  E    L+ E RNLLSV YKNVVG++R +WR 
Sbjct: 189 SRNELVQLAKLAEQTERFEDVILYMKKAIEINPSLNKEHRNLLSVGYKNVVGSKRFAWRH 248

Query: 297 ISSIEQKTEGSERKQQMAK--EYRVKVEKELREICYDVL 407
           +     ++    R  Q+    +Y+ K+E EL+ +C ++L
Sbjct: 249 LHHDALQSGRYIRDSQLKGIIKYKEKIEMELKTLCREIL 287


>SB_23775| Best HMM Match : Tropomyosin (HMM E-Value=0)
          Length = 442

 Score = 32.3 bits (70), Expect = 0.43
 Identities = 21/79 (26%), Positives = 37/79 (46%)
 Frame = +3

Query: 141 AKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKT 320
           AK  ++ ER +  A   K+  E   +   EE++ L    K     R+    + + IE+K 
Sbjct: 346 AKQKKEQERLEKQAEKEKKEKERLEKKQREEKDRLEKKEKKEEEKRKKEEEINAKIEEKK 405

Query: 321 EGSERKQQMAKEYRVKVEK 377
           +  E+K+Q  +E   K E+
Sbjct: 406 KREEKKKQEEEEKMKKKEQ 424


>SB_33101| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 394

 Score = 29.9 bits (64), Expect = 2.3
 Identities = 20/59 (33%), Positives = 25/59 (42%), Gaps = 1/59 (1%)
 Frame = +3

Query: 87  VLFHRPRCPSTRXELVQRAKLAEQAERYDDMAAAMKEVT-ETGVELSNEERNLLSVAYK 260
           V F  P CP TR     R  +A     Y    A MK +  + G ELS E+  +   A K
Sbjct: 314 VYFTTPPCPGTRATKPFRENIAALVVFYTPSKADMKAILHDYGAELSEEKMKVYMKALK 372


>SB_11347| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1234

 Score = 29.1 bits (62), Expect = 4.0
 Identities = 27/112 (24%), Positives = 50/112 (44%), Gaps = 6/112 (5%)
 Frame = +3

Query: 129 LVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGAR------RSSW 290
           L +  K A   +R   +A+A+ +     +   +     LS   K VV  +      +  W
Sbjct: 282 LSEMFKQAVTEDRPKMLASAITKALNQDIHSPSALNLPLSTPAKQVVKTKWDRIREKHQW 341

Query: 291 RVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASN 446
            ++S  E+    +ER++Q   E +VK E++ R+   ++  +   HL  K SN
Sbjct: 342 NLLSEDEKNKIKAERRKQKRLELKVKREEKERKRLEELKRVSFAHLGMKLSN 393


>SB_47927| Best HMM Match : TSP_1 (HMM E-Value=9.5e-34)
          Length = 512

 Score = 28.3 bits (60), Expect = 7.0
 Identities = 20/85 (23%), Positives = 43/85 (50%), Gaps = 1/85 (1%)
 Frame = +3

Query: 138 RAKLAEQAERYDDMAAAM-KEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVISSIEQ 314
           + K+ +++E+YD +   + KE T+ G   +  E    +    N   AR  +    ++ + 
Sbjct: 13  KRKIFKKSEKYDRLEEPLEKEGTDGGEIEAEAEVEPHTEEEANYNEARSENCEKNNNNDS 72

Query: 315 KTEGSERKQQMAKEYRVKVEKELRE 389
            T  ++ +Q   KE+ V +EK+L++
Sbjct: 73  STPDAKSQQADIKEWIVSLEKQLKD 97


>SB_15350| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1126

 Score = 28.3 bits (60), Expect = 7.0
 Identities = 13/35 (37%), Positives = 22/35 (62%)
 Frame = +3

Query: 291  RVISSIEQKTEGSERKQQMAKEYRVKVEKELREIC 395
            R++  IE+K E  E  ++ AKE + + E+E + IC
Sbjct: 919  RIMKEIEEK-EKKEEAERKAKEEKEREERERKRIC 952


>SB_12646| Best HMM Match : SSrecog (HMM E-Value=0)
          Length = 783

 Score = 28.3 bits (60), Expect = 7.0
 Identities = 11/34 (32%), Positives = 22/34 (64%)
 Frame = +3

Query: 336 KQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 437
           K++M ++Y  K+EKE+    Y+++  L K ++ K
Sbjct: 292 KEEMKEKYDGKIEKEMSGAIYEIISRLMKAVVGK 325


>SB_10602| Best HMM Match : RVT_1 (HMM E-Value=1.3e-10)
          Length = 416

 Score = 27.9 bits (59), Expect = 9.3
 Identities = 15/62 (24%), Positives = 30/62 (48%)
 Frame = +3

Query: 402 VLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEIS 581
           +LGL++ H+ PK+ +   ++F      +Y+ +L  V    T+   +E +   Y    + S
Sbjct: 116 ILGLIESHIDPKSFH--HRMFLAACCLEYFGFLRSVEFTVTQEKCIEQNMPLYAVFIDFS 173

Query: 582 XA 587
            A
Sbjct: 174 KA 175


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 22,570,781
Number of Sequences: 59808
Number of extensions: 434921
Number of successful extensions: 1181
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1088
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1175
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 2034222073
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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