Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0470
(750 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 329 1e-90
At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 329 1e-90
At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 329 1e-90
At1g29760.1 68414.m03639 expressed protein 31 0.82
At4g10710.1 68417.m01751 transcriptional regulator-related simil... 30 1.9
At4g38440.1 68417.m05432 expressed protein 29 2.5
At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 3.3
At5g25150.1 68418.m02981 transducin family protein / WD-40 repea... 28 5.8
At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 28 5.8
At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 28 5.8
At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 28 5.8
At1g35820.1 68414.m04451 hypothetical protein 28 5.8
At3g30520.1 68416.m03863 hypothetical protein 28 7.6
At2g26730.1 68415.m03206 leucine-rich repeat transmembrane prote... 28 7.6
>At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal
protein S4, Arabidopsis thaliana, PIR:T48480
Length = 262
Score = 329 bits (809), Expect = 1e-90
Identities = 149/217 (68%), Positives = 179/217 (82%)
Frame = +1
Query: 31 PKHGMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIKVDG 210
PKH MLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+VDG
Sbjct: 15 PKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDG 74
Query: 211 KVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 390
KVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K
Sbjct: 75 KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134
Query: 391 NVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGRVGT 570
+PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+ GN+ M+TGGRN GRVG
Sbjct: 135 GIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNVVMVTGGRNRGRVGV 194
Query: 571 IVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIRQG 681
I +RE+H GSF+ +HI+DSTGH FATRL NV+ I +G
Sbjct: 195 IKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGKG 231
Score = 36.3 bits (80), Expect = 0.022
Identities = 15/23 (65%), Positives = 18/23 (78%)
Frame = +2
Query: 674 GKGTKAYISLPXGKGXRLTXXEE 742
GKGTK ++SLP GKG +LT EE
Sbjct: 229 GKGTKPWVSLPKGKGIKLTIIEE 251
Score = 31.1 bits (67), Expect = 0.82
Identities = 12/13 (92%), Positives = 12/13 (92%)
Frame = +3
Query: 3 KKHLKRLNAPKAW 41
KKHLKRLNAPK W
Sbjct: 6 KKHLKRLNAPKHW 18
>At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B)
Length = 262
Score = 329 bits (808), Expect = 1e-90
Identities = 149/217 (68%), Positives = 179/217 (82%)
Frame = +1
Query: 31 PKHGMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIKVDG 210
PKH MLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+VDG
Sbjct: 15 PKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDG 74
Query: 211 KVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 390
KVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K
Sbjct: 75 KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134
Query: 391 NVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGRVGT 570
+PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+ GN+ M+TGGRN GRVG
Sbjct: 135 GIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGV 194
Query: 571 IVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIRQG 681
I +RE+H GSF+ +HI+DSTGH FATRL NV+ I +G
Sbjct: 195 IKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGKG 231
Score = 36.3 bits (80), Expect = 0.022
Identities = 15/23 (65%), Positives = 18/23 (78%)
Frame = +2
Query: 674 GKGTKAYISLPXGKGXRLTXXEE 742
GKGTK ++SLP GKG +LT EE
Sbjct: 229 GKGTKPWVSLPKGKGIKLTIIEE 251
Score = 31.1 bits (67), Expect = 0.82
Identities = 12/13 (92%), Positives = 12/13 (92%)
Frame = +3
Query: 3 KKHLKRLNAPKAW 41
KKHLKRLNAPK W
Sbjct: 6 KKHLKRLNAPKHW 18
>At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains
ribosomal protein S4 signature from residues 8 to 22
Length = 261
Score = 329 bits (808), Expect = 1e-90
Identities = 149/217 (68%), Positives = 179/217 (82%)
Frame = +1
Query: 31 PKHGMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVLKIVKQRLIKVDG 210
PKH MLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ I+ QR I+VDG
Sbjct: 15 PKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVISILMQRHIQVDG 74
Query: 211 KVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPK 390
KVRTD TYPAGFMDVVSI KTNE FRL+YD KGRF +H I EEAK+KLCKV+ + G K
Sbjct: 75 KVRTDKTYPAGFMDVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQK 134
Query: 391 NVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITGGRNLGRVGT 570
+PYL T+DGRTIRYPDPLIK ND+I+LD+ KI++FIKF+ GN+ M+TGGRN GRVG
Sbjct: 135 GIPYLNTYDGRTIRYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVVMVTGGRNRGRVGV 194
Query: 571 IVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIRQG 681
I +RE+H GSF+ +HI+DSTGH FATRL NV+ I +G
Sbjct: 195 IKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGKG 231
Score = 36.3 bits (80), Expect = 0.022
Identities = 15/23 (65%), Positives = 18/23 (78%)
Frame = +2
Query: 674 GKGTKAYISLPXGKGXRLTXXEE 742
GKGTK ++SLP GKG +LT EE
Sbjct: 229 GKGTKPWVSLPKGKGIKLTIIEE 251
Score = 31.1 bits (67), Expect = 0.82
Identities = 12/13 (92%), Positives = 12/13 (92%)
Frame = +3
Query: 3 KKHLKRLNAPKAW 41
KKHLKRLNAPK W
Sbjct: 6 KKHLKRLNAPKHW 18
>At1g29760.1 68414.m03639 expressed protein
Length = 526
Score = 31.1 bits (67), Expect = 0.82
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -2
Query: 224 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 132
S+LTFP +R CF F F + RRFL
Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225
>At4g10710.1 68417.m01751 transcriptional regulator-related similar
to chromatin-specific transcription elongation factor
FACT 140 kDa subunit (GI:5499741) [Homo sapiens]
Length = 1074
Score = 29.9 bits (64), Expect = 1.9
Identities = 11/20 (55%), Positives = 13/20 (65%)
Frame = -2
Query: 113 KHSRNLWGPVDGLGAYTPPS 54
KHS +LWG D L TPP+
Sbjct: 44 KHSADLWGSADALAIATPPA 63
>At4g38440.1 68417.m05432 expressed protein
Length = 1465
Score = 29.5 bits (63), Expect = 2.5
Identities = 25/100 (25%), Positives = 43/100 (43%), Gaps = 4/100 (4%)
Frame = +1
Query: 28 LPKHGMLDKLGGVYAPRPSTGPHKLRECLPL----VIFLRNRLKYALTGNEVLKIVKQRL 195
LP H L + V++ + STGP + E L + V FL + G+ +V L
Sbjct: 1144 LPPHWFLSAISAVHSGKTSTGPPESTELLEVAKAGVFFLAGLESSSGFGSLPSPVVSVPL 1203
Query: 196 IKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRF 315
+ K T MD++ + T L+ + ++ G+F
Sbjct: 1204 V---WKFHALSTVLLVGMDIIEDKNTRNLYNYLQELYGQF 1240
>At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase,
putative / steroid 5-alpha-reductase, putative identical
to gi:1280611; contains a steroid 5-alpha reductase,
C-terminal domain
Length = 262
Score = 29.1 bits (62), Expect = 3.3
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = -2
Query: 203 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 87
TF R C + P A +FL+ GKH+R WGP
Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45
>At5g25150.1 68418.m02981 transducin family protein / WD-40 repeat
family protein similar to TBP-associated factor
(GI:1732075) [Homo sapiens] and to 100 kDa subunit of
Pol II transcription factor (GI:1491718) {Homo sapiens];
contains Pfam PF00400: WD domain, G-beta repeat (6
copies)|8689032|gb|AV528749.1|AV528749
Length = 666
Score = 28.3 bits (60), Expect = 5.8
Identities = 26/100 (26%), Positives = 43/100 (43%), Gaps = 7/100 (7%)
Frame = +1
Query: 406 VTHDGRTIR--YPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITGGRN----LGRVG 567
++HDG + + D IKV D ++ A + + S G R+ LG G
Sbjct: 360 ISHDGSLVAGGFSDSSIKVWDMAKIGQAGSGALQAENDSSDQSIGPNGRRSYTLLLGHSG 419
Query: 568 TIVSRE-RHPGSFDIVHIKDSTGHTFATRLNNVFIIRQGH 684
+ S PG F + D+T ++T+LN + +GH
Sbjct: 420 PVYSATFSPPGDFVLSSSADTTIRLWSTKLNANLVCYKGH 459
>At4g08180.3 68417.m01353 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 5.8
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +1
Query: 136 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 270
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.2 68417.m01352 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 813
Score = 28.3 bits (60), Expect = 5.8
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +1
Query: 136 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 270
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 748
>At4g08180.1 68417.m01351 oxysterol-binding family protein similar
to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens};
contains Pfam profiles PF00169: PH domain, PF01237:
Oxysterol-binding protein
Length = 814
Score = 28.3 bits (60), Expect = 5.8
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +1
Query: 136 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY-PAGFMDVVSIEK 270
N ++A+T NE+ +K+RL D ++R D Y G ++ + EK
Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEK 749
>At1g35820.1 68414.m04451 hypothetical protein
Length = 287
Score = 28.3 bits (60), Expect = 5.8
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +3
Query: 21 LNAPKAWXVGQTWRCVCTETVNRSPQV 101
+NA AW Q + C T+++NR P V
Sbjct: 34 INASDAWWTEQEFGCKLTKSLNRKPPV 60
>At3g30520.1 68416.m03863 hypothetical protein
Length = 397
Score = 27.9 bits (59), Expect = 7.6
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = +3
Query: 21 LNAPKAWXVGQTWRCVCTETVNRSPQV 101
+NA AW Q + C T+ +NR P V
Sbjct: 62 INASDAWWTEQEFGCKLTKNLNRKPPV 88
>At2g26730.1 68415.m03206 leucine-rich repeat transmembrane protein
kinase, putative
Length = 658
Score = 27.9 bits (59), Expect = 7.6
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +1
Query: 412 HDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNLCMITG 543
H+ + +P ++N+ I+LDI++ I F NL +TG
Sbjct: 124 HNEFSGEFPTSFTQLNNLIRLDISSNNFTGSIPFSVNNLTHLTG 167
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,245,231
Number of Sequences: 28952
Number of extensions: 388975
Number of successful extensions: 1138
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1075
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1136
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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