Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0445
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11E3.07 |vma4||V-type ATPase subunit E|Schizosaccharomyces p... 122 7e-29
SPBC3B9.09 |vps36||RBZ zinc finger protein Vps36|Schizosaccharom... 31 0.23
SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 28 1.2
SPBC215.10 |||haloacid dehalogenase-like hydrolase|Schizosacchar... 27 2.9
SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||M... 27 2.9
SPAC824.05 |vps16||HOPS complex subunit Vps16 |Schizosaccharomyc... 26 5.0
SPAC637.06 |||alpha-1,2-galactosyltransferase |Schizosaccharomyc... 26 6.6
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 26 6.6
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 26 6.6
>SPAC11E3.07 |vma4||V-type ATPase subunit E|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 227
Score = 122 bits (293), Expect = 7e-29
Identities = 67/210 (31%), Positives = 111/210 (52%), Gaps = 1/210 (0%)
Frame = +1
Query: 13 MAFIEQXXXXXXXXXXXXXXXXFNIEKGRLVQQQRLKIMXXXXXXXXXXXXXXXIQSSNM 192
++FI+Q F +EK ++V++Q I I SN+
Sbjct: 16 VSFIKQEALEKAKEIHTLSEEEFQVEKAKIVREQCDAIDQTYDMKLKRASMAQKIAKSNV 75
Query: 193 LNQARLKVLKVREDHVRNVLDEARKRLAEVPKDTKLYSELLVTLIVQALFQLMEPTVTIR 372
LN++RL++L ++ + ++ K+L + + Y++ + LIVQA+ L EP +
Sbjct: 76 LNKSRLEILNSKQKVIDDIFSRVEKKLDGIEQKKDAYTKFMADLIVQAMELLGEPVGIVY 135
Query: 373 VRQTDKALVESLLGKAQTDYKNKIKK-DVVLKVDTENFLSPDTCGGIELVAARGRIKISN 549
RQ D +V++ + KA K+K D L +T++FL+ GG+ LV G+I++ N
Sbjct: 136 SRQRDAEIVKAAIPKATEVLKSKNGSIDYELDAETDDFLNDSVLGGVVLVGLGGKIRVDN 195
Query: 550 TLESRLELIAQQLLPEIRNALFGRNPNRKF 639
TL +RLE++ ++ LPEIR LFG NPNRKF
Sbjct: 196 TLRARLEIVKEEALPEIRRLLFGENPNRKF 225
>SPBC3B9.09 |vps36||RBZ zinc finger protein
Vps36|Schizosaccharomyces pombe|chr 2|||Manual
Length = 467
Score = 30.7 bits (66), Expect = 0.23
Identities = 15/41 (36%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -3
Query: 502 HRCRATKSS-RCRLSTQHPS*SYSCSLFGLFRAGTPPEPCR 383
H R +SS + RLS +H S++C + G P PCR
Sbjct: 77 HTSRYFRSSPKIRLSLRHVEKSWACKICTFINVGDPINPCR 117
>SPBC29A10.10c |||tRNA-splicing endonuclease positive effector
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1944
Score = 28.3 bits (60), Expect = 1.2
Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 2/68 (2%)
Frame = +1
Query: 382 TDKALVESLLGKAQTDYKNKIKKDVVLKVDTENF--LSPDTCGGIELVAARGRIKISNTL 555
TD+ ++ + K + D + K+DV+ K+D S G +E +G +K S
Sbjct: 1861 TDQTAADNKVTKPRLDESSSSKQDVLNKIDESEIEQASSKKPGYVEKNKDKGHMKKSKKP 1920
Query: 556 ESRLELIA 579
+S+L L A
Sbjct: 1921 KSKLALAA 1928
>SPBC215.10 |||haloacid dehalogenase-like
hydrolase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 302
Score = 27.1 bits (57), Expect = 2.9
Identities = 15/42 (35%), Positives = 24/42 (57%)
Frame = +1
Query: 232 DHVRNVLDEARKRLAEVPKDTKLYSELLVTLIVQALFQLMEP 357
D N L+EA+KRLA +P D E+ +T + F+++ P
Sbjct: 181 DDDTNGLEEAKKRLAGIPSD-----EVALTQALPQTFEIIPP 217
>SPBC27.04 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1052
Score = 27.1 bits (57), Expect = 2.9
Identities = 28/93 (30%), Positives = 40/93 (43%), Gaps = 9/93 (9%)
Frame = +1
Query: 250 LDEARKRLAEVPKDTK-LYSELLVTLIVQALFQL---MEPTVTIRVR-----QTDKALVE 402
++EA K KD K L +L T+ + + +E T+TI V DK E
Sbjct: 745 VEEAEKDYQPSEKDDKSLIGGILSTVEMTQSTDVNDEIENTLTIPVEVEHSDNIDKGSEE 804
Query: 403 SLLGKAQTDYKNKIKKDVVLKVDTENFLSPDTC 501
+ LGK D N + DV+ V E + D C
Sbjct: 805 NDLGKEAVDEANNNQNDVLESVVVEQSTTTDDC 837
>SPAC824.05 |vps16||HOPS complex subunit Vps16 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 835
Score = 26.2 bits (55), Expect = 5.0
Identities = 16/42 (38%), Positives = 21/42 (50%)
Frame = -1
Query: 558 LQSVADLDTSPGCNQLDSTTGVGRQKVLGVDFQHNILLDLIL 433
LQ V+ + SP L VG+ +V+ DF LLDL L
Sbjct: 223 LQHVSHISISPNARYLALYESVGKVRVISSDFSKE-LLDLRL 263
>SPAC637.06 |||alpha-1,2-galactosyltransferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 347
Score = 25.8 bits (54), Expect = 6.6
Identities = 13/39 (33%), Positives = 21/39 (53%), Gaps = 1/39 (2%)
Frame = -1
Query: 528 PGC-NQLDSTTGVGRQKVLGVDFQHNILLDLILVVCLGF 415
PG N LDS G R+K H + + ++L++C G+
Sbjct: 22 PGTSNVLDSKEGDTRRKYFTKTHLHRLFVFVVLLLCSGY 60
>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2310
Score = 25.8 bits (54), Expect = 6.6
Identities = 15/48 (31%), Positives = 26/48 (54%)
Frame = +1
Query: 502 GGIELVAARGRIKISNTLESRLELIAQQLLPEIRNALFGRNPNRKFTD 645
G + L +R I+ N + L+L++QQ+ + NAL ++ R TD
Sbjct: 1562 GALYLCLSRRAIRTGNV--TFLKLLSQQIAISVSNALLFQSLRRTITD 1607
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 25.8 bits (54), Expect = 6.6
Identities = 17/49 (34%), Positives = 25/49 (51%)
Frame = -1
Query: 435 LVVCLGFSEQGLHQSLVGLTDADGDSGFHELEESLHNKCDQQL*VQFGV 289
L+V EQG ++ D+ +SG HE+ + L N DQQL Q +
Sbjct: 492 LIVACEELEQGFDLDIL---DSLRESGIHEVIQLLRNFPDQQLEKQLNI 537
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,693,165
Number of Sequences: 5004
Number of extensions: 52367
Number of successful extensions: 171
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 163
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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