Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0439
(750 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_UPI0000DB72ED Cluster: PREDICTED: similar to CG33950-PD... 49 1e-04
UniRef50_Q06561 Cluster: Basement membrane proteoglycan precurso... 44 0.003
UniRef50_P98160 Cluster: Basement membrane-specific heparan sulf... 41 0.037
UniRef50_UPI000051A338 Cluster: PREDICTED: similar to agrin isof... 37 0.61
UniRef50_Q28XG9 Cluster: GA21051-PA; n=1; Drosophila pseudoobscu... 36 0.81
UniRef50_UPI0000DB6FF3 Cluster: PREDICTED: similar to SP2353 CG8... 36 1.4
UniRef50_UPI00015546F0 Cluster: PREDICTED: similar to hCG1651100... 35 2.5
UniRef50_Q67L58 Cluster: Putative uncharacterized protein; n=1; ... 34 3.3
UniRef50_P17255 Cluster: Vacuolar ATP synthase catalytic subunit... 34 3.3
UniRef50_UPI00006108E4 Cluster: OTTHUMP00000016676; n=2; Gallus ... 33 7.5
UniRef50_Q6UE74 Cluster: ORF2; n=24; Tetrahymena thermophila|Rep... 33 7.5
UniRef50_UPI0000E474D2 Cluster: PREDICTED: similar to agrin; n=1... 33 9.9
UniRef50_Q4S6A5 Cluster: Chromosome 9 SCAF14729, whole genome sh... 33 9.9
UniRef50_Q4RQW3 Cluster: Chromosome 14 SCAF15003, whole genome s... 33 9.9
UniRef50_Q11WT3 Cluster: DTDP-glucose 4,6 dehydratase; n=1; Cyto... 33 9.9
UniRef50_Q9NGV2 Cluster: SP2353; n=2; Drosophila melanogaster|Re... 33 9.9
UniRef50_Q37382 Cluster: NADH-ubiquinone oxidoreductase chain 3;... 33 9.9
>UniRef50_UPI0000DB72ED Cluster: PREDICTED: similar to CG33950-PD,
isoform D; n=1; Apis mellifera|Rep: PREDICTED: similar to
CG33950-PD, isoform D - Apis mellifera
Length = 3382
Score = 49.2 bits (112), Expect = 1e-04
Identities = 28/91 (30%), Positives = 47/91 (51%), Gaps = 3/91 (3%)
Frame = +2
Query: 5 RHQVAA-RLHEDGHAWLSVDGVDKSAPATGISNVMNADSNIYIGGIPSWLNIHG--YPGF 175
RH++ R DG L+ + ++ S + G +N N+Y+GG+P + +G GF
Sbjct: 3221 RHRIILKRQGSDGSIELNGEHMESST-SYGTQQDLNTKGNVYLGGVPDYAMTYGKYQEGF 3279
Query: 176 IGCIENVETTDSHRGLNLRDTAVAGRNTQLC 268
GCI +E DS +++ D A+ G+N C
Sbjct: 3280 SGCIYTMEVQDS-GAIDIGDKAIRGKNVSPC 3309
>UniRef50_Q06561 Cluster: Basement membrane proteoglycan precursor;
n=8; Eukaryota|Rep: Basement membrane proteoglycan
precursor - Caenorhabditis elegans
Length = 3375
Score = 44.4 bits (100), Expect = 0.003
Identities = 24/65 (36%), Positives = 36/65 (55%), Gaps = 3/65 (4%)
Frame = +2
Query: 83 ATGISNVMNADSNIYIGGIPSWLNIHG---YPGFIGCIENVETTDSHRGLNLRDTAVAGR 253
+TGI ++N D NI++GG+P G F+GCI +VE L+L TA+ G+
Sbjct: 3297 STGILAMLNVDGNIFVGGVPDISKATGGLFSNNFVGCIADVELNGVK--LDLMATAIDGK 3354
Query: 254 NTQLC 268
N + C
Sbjct: 3355 NVKPC 3359
>UniRef50_P98160 Cluster: Basement membrane-specific heparan sulfate
proteoglycan core protein precursor; n=26; Eumetazoa|Rep:
Basement membrane-specific heparan sulfate proteoglycan
core protein precursor - Homo sapiens (Human)
Length = 4391
Score = 40.7 bits (91), Expect = 0.037
Identities = 31/97 (31%), Positives = 47/97 (48%), Gaps = 10/97 (10%)
Frame = +2
Query: 8 HQVAARLHEDGHAWLSVDGVDK-SAPATGISNVMNADSNIYIGGIPSWLNIHG---YPGF 175
H+V A L E + VDG + S + G + +NA ++YIGG P + G G
Sbjct: 4294 HRVTA-LREGRRGSIQVDGEELVSGRSPGPNVAVNAKGSVYIGGAPDVATLTGGRFSSGI 4352
Query: 176 IGCIENVETTDSHRG------LNLRDTAVAGRNTQLC 268
GC++N+ + G L+L+ A AG NT+ C
Sbjct: 4353 TGCVKNLVLHSARPGAPPPQPLDLQHRAQAGANTRPC 4389
>UniRef50_UPI000051A338 Cluster: PREDICTED: similar to agrin isoform
1; n=1; Apis mellifera|Rep: PREDICTED: similar to agrin
isoform 1 - Apis mellifera
Length = 2397
Score = 36.7 bits (81), Expect = 0.61
Identities = 26/88 (29%), Positives = 45/88 (51%), Gaps = 7/88 (7%)
Frame = +2
Query: 2 TRHQVAA-RLHEDGHAWLSVDGVDKSAPATGISNVMNADSNIYIGGIPS-----WLNIHG 163
T H VAA R H+DG + DG D + G+ ++ + + ++G +P+ + NI
Sbjct: 1616 TFHSVAAKRYHKDG-VLIFNDGEDVVGQSQGMLKSLDLNQDTFVGNMPTNYSKVYENIGT 1674
Query: 164 YPGFIGCIENVETTDSHRGLNL-RDTAV 244
GF+GCI ++ H L++ RD +
Sbjct: 1675 NHGFLGCIRKLKINRIHVDLHVGRDKEI 1702
>UniRef50_Q28XG9 Cluster: GA21051-PA; n=1; Drosophila
pseudoobscura|Rep: GA21051-PA - Drosophila pseudoobscura
(Fruit fly)
Length = 1276
Score = 36.3 bits (80), Expect = 0.81
Identities = 21/67 (31%), Positives = 33/67 (49%), Gaps = 4/67 (5%)
Frame = +2
Query: 8 HQVAARLHEDGHAWLSVDGVDKSAP-ATGISNVMNADSNIYIGGIPS---WLNIHGYPGF 175
H+V A + +L VDG S A G +N D+ +Y+GG+P + + Y G
Sbjct: 1185 HRVRA-IRNSQEGYLEVDGRKTSTQRAPGKLRQLNTDTGLYVGGMPDVAYFTHRRYYSGI 1243
Query: 176 IGCIENV 196
+GCI +
Sbjct: 1244 VGCISEI 1250
>UniRef50_UPI0000DB6FF3 Cluster: PREDICTED: similar to SP2353
CG8403-PA; n=2; Apocrita|Rep: PREDICTED: similar to
SP2353 CG8403-PA - Apis mellifera
Length = 929
Score = 35.5 bits (78), Expect = 1.4
Identities = 26/90 (28%), Positives = 40/90 (44%), Gaps = 3/90 (3%)
Frame = +2
Query: 8 HQVAARLHEDGHAWLSVDGVDKSAPATGISNVMNADSNIYIGGIPSWLNIHG---YPGFI 178
H+V A +E + + G SA + G +N D+ +Y+GG P + G G +
Sbjct: 840 HRVRAVRNEQWASLVVDSGTGVSASSPGQLRQLNTDTGLYVGGAPDIVRTTGGRYAKGIV 899
Query: 179 GCIENVETTDSHRGLNLRDTAVAGRNTQLC 268
GCI ++ DS + L A NT C
Sbjct: 900 GCISDL-VLDSDFSVALSSPGQA-TNTHSC 927
>UniRef50_UPI00015546F0 Cluster: PREDICTED: similar to hCG1651100;
n=1; Ornithorhynchus anatinus|Rep: PREDICTED: similar to
hCG1651100 - Ornithorhynchus anatinus
Length = 749
Score = 34.7 bits (76), Expect = 2.5
Identities = 23/82 (28%), Positives = 42/82 (51%), Gaps = 6/82 (7%)
Frame = +2
Query: 8 HQVAARLHEDGHAWLSVDGVDKSAPATGISNVMNADSNIYIGGIPSWLNIHGYP------ 169
H + A H +L++DG + + ATG V++ +++ Y+GG+ S +++
Sbjct: 394 HLIKAGRHGT-EGYLTLDGTNVTQKATGRMTVLDTNTDFYVGGVSSLDSVNSMAVENDPV 452
Query: 170 GFIGCIENVETTDSHRGLNLRD 235
GF GC+ E ++R L L D
Sbjct: 453 GFDGCVR--EIFINNRELKLTD 472
>UniRef50_Q67L58 Cluster: Putative uncharacterized protein; n=1;
Symbiobacterium thermophilum|Rep: Putative
uncharacterized protein - Symbiobacterium thermophilum
Length = 384
Score = 34.3 bits (75), Expect = 3.3
Identities = 16/50 (32%), Positives = 22/50 (44%)
Frame = -3
Query: 244 HSGVSQIQSSVAVRCLNILNATDEAWISVDVQPAWYSTDVYVGVSVHDVG 95
HS + R +LN D A + V+ P WY+ DVY V+ G
Sbjct: 2 HSAQLEYAEHSPARIRALLNRIDPAAVGVETPPQWYAEDVYFEVAYESYG 51
>UniRef50_P17255 Cluster: Vacuolar ATP synthase catalytic subunit A
(EC 3.6.3.14) (V-ATPase subunit A) (Vacuolar proton pump
subunit A) [Contains: Endonuclease PI-SceI (EC 3.1.-.-)
(VMA1-derived endonuclease) (VDE) (Sce VMA intein)];
n=14; Ascomycota|Rep: Vacuolar ATP synthase catalytic
subunit A (EC 3.6.3.14) (V-ATPase subunit A) (Vacuolar
proton pump subunit A) [Contains: Endonuclease PI-SceI
(EC 3.1.-.-) (VMA1-derived endonuclease) (VDE) (Sce VMA
intein)] - Saccharomyces cerevisiae (Baker's yeast)
Length = 1071
Score = 34.3 bits (75), Expect = 3.3
Identities = 19/61 (31%), Positives = 29/61 (47%)
Frame = +2
Query: 62 GVDKSAPATGISNVMNADSNIYIGGIPSWLNIHGYPGFIGCIENVETTDSHRGLNLRDTA 241
G K+ + +S N+D+ IY+G N+ G I CIEN+E + G + R
Sbjct: 260 GCGKTVISQSLSKYSNSDAIIYVGCFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPRE 319
Query: 242 V 244
V
Sbjct: 320 V 320
>UniRef50_UPI00006108E4 Cluster: OTTHUMP00000016676; n=2; Gallus
gallus|Rep: OTTHUMP00000016676 - Gallus gallus
Length = 398
Score = 33.1 bits (72), Expect = 7.5
Identities = 20/70 (28%), Positives = 36/70 (51%), Gaps = 7/70 (10%)
Frame = +2
Query: 47 WLSVDGVDKSAPATGISNVMNADSNIYIGGIPSWLNI------HGYP-GFIGCIENVETT 205
++ +DG++ + A+ NV++ ++ Y+GG+ S LN+ P GF GCI +
Sbjct: 51 YVDLDGINITHTASAGMNVLDTHTDFYVGGVSS-LNLVNSMATENEPTGFSGCIREIVIN 109
Query: 206 DSHRGLNLRD 235
D L + D
Sbjct: 110 DKELKLTVTD 119
>UniRef50_Q6UE74 Cluster: ORF2; n=24; Tetrahymena thermophila|Rep:
ORF2 - Tetrahymena thermophila
Length = 1147
Score = 33.1 bits (72), Expect = 7.5
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = -1
Query: 159 WMFNQLGIPPMYMLESAFMTLEIPVAGALLSTPSTESQACPSSCR 25
W +N+LG+ P +L F+ ++ A A++ T S +S+ C S R
Sbjct: 914 WRYNKLGVIPCLLLWQLFIKSQLQYASAIMIT-SAQSEKCMRSLR 957
>UniRef50_UPI0000E474D2 Cluster: PREDICTED: similar to agrin; n=1;
Strongylocentrotus purpuratus|Rep: PREDICTED: similar to
agrin - Strongylocentrotus purpuratus
Length = 1397
Score = 32.7 bits (71), Expect = 9.9
Identities = 27/96 (28%), Positives = 47/96 (48%), Gaps = 7/96 (7%)
Frame = +2
Query: 2 TRHQVAA-RLHEDGHAWLSVDGVDK-SAPATGISNVMNADSNIYIG-GIPSWLNI---HG 163
T H++ A R +G LSVDG + + + G+S +N +++IG +P + + +
Sbjct: 1144 TWHRLRAYRSRREGS--LSVDGEPEVTGTSEGVSGALNLGEDLFIGYAVPPEVGLRLANT 1201
Query: 164 YPGFIGCIENVETTDSHRGLNLRDTAVA-GRNTQLC 268
GF+GCI VE ++ ++V G N C
Sbjct: 1202 NQGFVGCIRYVEINSQELDISSSGSSVEYGANVGEC 1237
>UniRef50_Q4S6A5 Cluster: Chromosome 9 SCAF14729, whole genome shotgun
sequence; n=3; Tetraodontidae|Rep: Chromosome 9
SCAF14729, whole genome shotgun sequence - Tetraodon
nigroviridis (Green puffer)
Length = 1693
Score = 32.7 bits (71), Expect = 9.9
Identities = 23/91 (25%), Positives = 42/91 (46%), Gaps = 6/91 (6%)
Frame = +2
Query: 14 VAARLHEDGHAWLSVDGVDKSAPATGISNVMNADSNIYIGGIPSWLNIHG---YPGFIGC 184
V R +DG+ + G + G S ++N ++Y+GG P + + G G GC
Sbjct: 1602 VLCRTGKDGYVQID-GGAAVHGQSRGRSIMVNTKGSVYLGGAPDMVAMTGGKFSAGLSGC 1660
Query: 185 IENVETTDS---HRGLNLRDTAVAGRNTQLC 268
+ N+ + + ++L+ A +G N Q C
Sbjct: 1661 VRNLVLMNGGQPAQTVDLQAHAASGFNVQPC 1691
>UniRef50_Q4RQW3 Cluster: Chromosome 14 SCAF15003, whole genome
shotgun sequence; n=2; Clupeocephala|Rep: Chromosome 14
SCAF15003, whole genome shotgun sequence - Tetraodon
nigroviridis (Green puffer)
Length = 1962
Score = 32.7 bits (71), Expect = 9.9
Identities = 18/64 (28%), Positives = 34/64 (53%), Gaps = 4/64 (6%)
Frame = +2
Query: 50 LSVDGVDKSAPATGI-SNVMNADSNIYIGGIPSWLN---IHGYPGFIGCIENVETTDSHR 217
L VDG A + SN + D IY+GG P+ ++ + F GC++N++ T + +
Sbjct: 1881 LVVDGEQSQAESPNARSNTCDTDDPIYVGGYPAGVHQAALSTSTSFRGCLKNLKITKASK 1940
Query: 218 GLNL 229
+++
Sbjct: 1941 TMDV 1944
>UniRef50_Q11WT3 Cluster: DTDP-glucose 4,6 dehydratase; n=1;
Cytophaga hutchinsonii ATCC 33406|Rep: DTDP-glucose 4,6
dehydratase - Cytophaga hutchinsonii (strain ATCC 33406
/ NCIMB 9469)
Length = 301
Score = 32.7 bits (71), Expect = 9.9
Identities = 18/58 (31%), Positives = 30/58 (51%)
Frame = -1
Query: 330 RDFLLNYFVVFVSMNSNQESLHSCVFRPATAVSRKFNPRWLSVVSTFSMQPMKPGYPW 157
RDF LN VF ++S ++++ SC F ++ + NP L + + P+ P Y W
Sbjct: 83 RDFHLNVLNVFNMLDSIRKNIPSCKFVNLSSAAVYGNPESLPIKEDTPINPLSP-YGW 139
>UniRef50_Q9NGV2 Cluster: SP2353; n=2; Drosophila melanogaster|Rep:
SP2353 - Drosophila melanogaster (Fruit fly)
Length = 1361
Score = 32.7 bits (71), Expect = 9.9
Identities = 19/67 (28%), Positives = 33/67 (49%), Gaps = 4/67 (5%)
Frame = +2
Query: 8 HQVAARLHEDGHAWLSVDGVDK-SAPATGISNVMNADSNIYIGGIPS---WLNIHGYPGF 175
H+V A + +L VDG + A G +N D+ +Y+GG+P + + + G
Sbjct: 1270 HRVRA-IRNSQEGYLEVDGRKTVTLRAPGKLRQLNTDTGLYVGGMPDVGYFTHQRYFSGI 1328
Query: 176 IGCIENV 196
+GCI +
Sbjct: 1329 VGCISEI 1335
>UniRef50_Q37382 Cluster: NADH-ubiquinone oxidoreductase chain 3;
n=4; Eukaryota|Rep: NADH-ubiquinone oxidoreductase chain
3 - Acanthamoeba castellanii (Amoeba)
Length = 118
Score = 32.7 bits (71), Expect = 9.9
Identities = 16/56 (28%), Positives = 28/56 (50%), Gaps = 2/56 (3%)
Frame = -1
Query: 318 LNYFVVFVSMNSNQESLHSCVFRPATAVSRKFNPRWLSVVSTFSMQPMKPGY--PW 157
L+YF+V+ + + S + C F+P KFN R+ + F + ++ Y PW
Sbjct: 25 LSYFLVYQESDIEKNSAYECGFQPFEDTRSKFNVRYYLIAILFMIFDLEIMYLFPW 80
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 648,441,059
Number of Sequences: 1657284
Number of extensions: 11858936
Number of successful extensions: 30983
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 29956
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 30970
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 61734884250
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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