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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0437
         (700 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_28550| Best HMM Match : Fer2_2 (HMM E-Value=2.49992e-42)           125   3e-29
SB_33375| Best HMM Match : ADK (HMM E-Value=1.6e-30)                  104   6e-23
SB_13785| Best HMM Match : No HMM Matches (HMM E-Value=.)              85   7e-17
SB_58530| Best HMM Match : No HMM Matches (HMM E-Value=.)              67   1e-11
SB_19245| Best HMM Match : ADK (HMM E-Value=0)                         67   1e-11
SB_31065| Best HMM Match : ADK (HMM E-Value=1.5e-31)                   53   2e-07
SB_4186| Best HMM Match : ADK (HMM E-Value=0.094)                      31   0.68 
SB_54131| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   1.6  
SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   8.4  

>SB_28550| Best HMM Match : Fer2_2 (HMM E-Value=2.49992e-42)
          Length = 1644

 Score =  125 bits (302), Expect = 3e-29
 Identities = 58/106 (54%), Positives = 76/106 (71%)
 Frame = +2

Query: 227 AMQKSGKERFLIDGFPRNQDNMDGWERVMSDKTKLLFVLFFECSREICTERCLRRGAAGS 406
           AM KS  ++FLIDGFPRN+DN+ GWE  M+DK  +  VLFFEC  +IC  R + RG + S
Sbjct: 1   AMAKSDVQKFLIDGFPRNEDNLQGWESRMNDKVDVKAVLFFECPEDICIGRIMSRGQS-S 59

Query: 407 GRSDDNIESLKKRFNTYLNDTMPIINYYDKLXLVRRINAEEEPDQV 544
           GR DDNIESL+KRF+T+ N TMPII +Y +  LV+ I+    PD++
Sbjct: 60  GRPDDNIESLRKRFHTHENSTMPIIEHYRRQGLVKPISGAPTPDEI 105


>SB_33375| Best HMM Match : ADK (HMM E-Value=1.6e-30)
          Length = 297

 Score =  104 bits (250), Expect = 6e-23
 Identities = 61/149 (40%), Positives = 90/149 (60%), Gaps = 3/149 (2%)
 Frame = +2

Query: 14  QVVFVLGAPGSGKGTQCSMISKEYDYVHLSAGDLLREERQRPGSEYGEMIEEKIRNGEIV 193
           ++V++LG PG+GKGT C  + K+Y+Y HLSAGDLLREE  R G+E G+MI E +R G++V
Sbjct: 117 RIVYILGGPGAGKGTICDSVVKKYNYCHLSAGDLLREEVTR-GNERGQMITEMMREGKLV 175

Query: 194 PVEVTCSLLHKAMQK-SGKERFLIDGFPRNQDNMDGWERVMSDKTKLLFVLFFECSREIC 370
           P E+T  LL  AM++      FLIDGFPR+ D    +E     + K L   F   + E+ 
Sbjct: 176 PKEITIGLLQDAMREHKDMPGFLIDGFPRDIDQGITFEEQHILRKKAL--NFLSSTAELP 233

Query: 371 TERC--LRRGAAGSGRSDDNIESLKKRFN 451
             +C    +G   +G+   + + + +RFN
Sbjct: 234 PLKCQIWSKGGPATGKK-THCKHVAERFN 261



 Score = 39.1 bits (87), Expect = 0.003
 Identities = 17/55 (30%), Positives = 30/55 (54%)
 Frame = +2

Query: 32  GAPGSGKGTQCSMISKEYDYVHLSAGDLLREERQRPGSEYGEMIEEKIRNGEIVP 196
           G P +GK T C  +++ ++Y H+  G L+ +E Q+      ++I + I  G  VP
Sbjct: 243 GGPATGKKTHCKHVAERFNYTHIMPGTLVAQEAQKDTDRAKQLI-QTISAGNDVP 296


>SB_13785| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 386

 Score = 84.6 bits (200), Expect = 7e-17
 Identities = 50/126 (39%), Positives = 73/126 (57%), Gaps = 1/126 (0%)
 Frame = +2

Query: 203 VTCSLLHKAMQKSGKER-FLIDGFPRNQDNMDGWERVMSDKTKLLFVLFFECSREICTER 379
           +   LL  AM K      FLIDGFPR  +    +E+ ++   +LL  L+FECS +    R
Sbjct: 2   IILELLRLAMVKQPNTTGFLIDGFPRELEQGLEFEKELA-LCELL--LYFECSPDTMKAR 58

Query: 380 CLRRGAAGSGRSDDNIESLKKRFNTYLNDTMPIINYYDKLXLVRRINAEEEPDQVFDKVK 559
            L+RG   SGR DDN E++  R  T++  TMP+I +Y +   V++I+AE  PD+VF KV 
Sbjct: 59  LLKRGET-SGRVDDNEETILSRLETFITQTMPVIEHYQEEGKVKKISAEGGPDEVFKKVS 117

Query: 560 VAFDEI 577
              +E+
Sbjct: 118 RVLEEL 123



 Score = 70.5 bits (165), Expect = 1e-12
 Identities = 55/201 (27%), Positives = 98/201 (48%), Gaps = 14/201 (6%)
 Frame = +2

Query: 17  VVFVLGAPGSGKGTQCSMISKEYDYVHLSAGDLLREERQRPGSEYGEMIEEKIRNGEIVP 196
           ++F++G PGSGK      +++     HLS G LL+EE  +  ++ G  + + I  G +V 
Sbjct: 136 IMFIVGGPGSGKAELAKRLAESSGRTHLSMGQLLKEEATK-DTDRGREVAQAIAMGTLVS 194

Query: 197 VEVTCSLLHKAMQKSGK--ERFLIDGFPRNQDNMDGWERVMSDKTKLLFVLFFECSREIC 370
            E   ++L + +    K  E +++DGFPR+ +    +   +SD  +   V+  ECS E  
Sbjct: 195 PEHALNILTEKIVSDEKNLEGYVVDGFPRSHEQAKNFTEKVSDFNR---VVALECSDEE- 250

Query: 371 TERCLRRGAAGSGRSDDNIESLKKRFNTYLNDTMPIIN------------YYDKLXLVRR 514
            ER L +   G+     N E LK R   Y ++T+ +++            Y     +V +
Sbjct: 251 MER-LAKEKLGAAL---NEEQLKTRIEVYRDNTIAMLDDIEGGKITKSSPYLPNNLVVIQ 306

Query: 515 INAEEEPDQVFDKVKVAFDEI 577
           I+AE  PD+ F ++   F+ +
Sbjct: 307 ISAEGGPDEAFKEISRVFEGV 327



 Score = 38.7 bits (86), Expect = 0.004
 Identities = 15/37 (40%), Positives = 24/37 (64%)
 Frame = +2

Query: 17  VVFVLGAPGSGKGTQCSMISKEYDYVHLSAGDLLREE 127
           +VF++G PGSGK     ++++     H+S G LL+EE
Sbjct: 340 IVFIVGGPGSGKTELAKLLAESSGRTHISMGQLLKEE 376


>SB_58530| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 288

 Score = 67.3 bits (157), Expect = 1e-11
 Identities = 49/184 (26%), Positives = 93/184 (50%), Gaps = 2/184 (1%)
 Frame = +2

Query: 14  QVVFVLGAPGSGKGTQCSMISKEYDYVHLSAGDLLREERQRPGSEYGEMIEEKIRNGEIV 193
           +V+FV+G PGSGK     ++++     H+S G LL+EE  +  ++    +   I  G +V
Sbjct: 105 RVIFVIGGPGSGKTELAKLLAESSGRTHISMGQLLKEEATK-DTDRAREVARAIGMGTLV 163

Query: 194 PVEVTCSLLHKAMQKSGKE--RFLIDGFPRNQDNMDGWERVMSDKTKLLFVLFFECSREI 367
             E    +L + + +  K    +++DG+PR+ +    +   +SD  +   V+  ECS E 
Sbjct: 164 SPEHALGILSETIVRGEKNLGGYVVDGYPRSHEQAKNFTEKVSDFNR---VVALECSDEE 220

Query: 368 CTERCLRRGAAGSGRSDDNIESLKKRFNTYLNDTMPIINYYDKLXLVRRINAEEEPDQVF 547
             ER L +   G+     N E LK R   Y ++T+ +++  ++     +I A +  ++VF
Sbjct: 221 -MER-LAKEKLGAAL---NEEQLKTRIEVYRDNTIAMLDDMEEAK-TTKIPAAQTIEEVF 274

Query: 548 DKVK 559
           ++ K
Sbjct: 275 EQTK 278


>SB_19245| Best HMM Match : ADK (HMM E-Value=0)
          Length = 188

 Score = 67.3 bits (157), Expect = 1e-11
 Identities = 48/136 (35%), Positives = 70/136 (51%), Gaps = 2/136 (1%)
 Frame = +2

Query: 11  PQVVFVLGAPGSGKGTQCSMISKEYDYVHLSAGDLLREERQRPGSEYGEMIEEKIRNGEI 190
           P    ++G PGSGKGT    I +++   HLS+G+LLR   Q   ++ GE  +  I  GE+
Sbjct: 31  PVRAIIMGPPGSGKGTVSERIVRDFGVEHLSSGELLRHHVQN-HTDLGEEAQAYILKGEL 89

Query: 191 VPVEVTCSL-LHKAMQKSGKERFLIDGFPRNQDNMDGWERVMSDKTKLLFVLFFECSREI 367
           VP E+   L LH+   K G   +L+DGFPR      G    +S+K K+  V+  +     
Sbjct: 90  VPDELMVDLILHELDAKKG--NWLLDGFPRTV----GQAETLSEKHKIDVVVNMDVPFAT 143

Query: 368 CTERCLRRGA-AGSGR 412
             +R  +R   A SGR
Sbjct: 144 IVDRIKQRWVHAVSGR 159


>SB_31065| Best HMM Match : ADK (HMM E-Value=1.5e-31)
          Length = 166

 Score = 53.2 bits (122), Expect = 2e-07
 Identities = 24/55 (43%), Positives = 34/55 (61%)
 Frame = +2

Query: 410 RSDDNIESLKKRFNTYLNDTMPIINYYDKLXLVRRINAEEEPDQVFDKVKVAFDE 574
           RSDDN  +L KR   Y + T P+I+YY K  +  +I+A + PD V+  V  AFD+
Sbjct: 103 RSDDNEATLTKRLQAYHSQTTPLIDYYTKRGIHHKIDASKPPDTVYSSVLKAFDD 157


>SB_4186| Best HMM Match : ADK (HMM E-Value=0.094)
          Length = 1053

 Score = 31.5 bits (68), Expect = 0.68
 Identities = 14/35 (40%), Positives = 23/35 (65%)
 Frame = +2

Query: 14  QVVFVLGAPGSGKGTQCSMISKEYDYVHLSAGDLL 118
           +VV + G PGS KG+  + + K + +V +SA DL+
Sbjct: 662 RVVIMFGGPGSTKGSIINDLVKMFGFVFISAEDLI 696


>SB_54131| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 3160

 Score = 30.3 bits (65), Expect = 1.6
 Identities = 15/49 (30%), Positives = 23/49 (46%)
 Frame = +2

Query: 134 RPGSEYGEMIEEKIRNGEIVPVEVTCSLLHKAMQKSGKERFLIDGFPRN 280
           RP  ++      +I   E V + + C+L+   + K G   FLI  FP N
Sbjct: 293 RPRFDFTADYLVRIYRAECVNLRLHCNLIKGVLDKQGYSAFLIASFPNN 341


>SB_15336| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 1068

 Score = 27.9 bits (59), Expect = 8.4
 Identities = 11/26 (42%), Positives = 16/26 (61%)
 Frame = -1

Query: 514 PADKT*FIVIVDDRHCIIQICVESFL 437
           P DK   +V VD  H  +Q+C+ +FL
Sbjct: 248 PTDKPRNVVFVDIGHSSLQVCITAFL 273


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,895,337
Number of Sequences: 59808
Number of extensions: 456623
Number of successful extensions: 1268
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 1171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1260
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 1829596184
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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