Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0422
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase |Schizosaccharom... 152 4e-38
SPBC902.05c |idh2|glu2|isocitrate dehydrogenase |Schizosaccharom... 121 9e-29
SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces ... 47 2e-06
SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase Le... 29 0.64
SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||M... 28 1.1
SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase Lac... 28 1.5
SPAC19G12.02c |pms1||MutL family mismatch-repair protein Pms1|Sc... 28 1.5
SPAC14C4.01c ||SPAC19D5.08c|DUF1770 family protein|Schizosacchar... 26 4.5
SPBP19A11.04c |mor2|cps12|morphogenesis protein Mor2|Schizosacch... 26 6.0
SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp... 25 7.9
>SPAC11G7.03 |idh1|glu3|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 356
Score = 152 bits (369), Expect = 4e-38
Identities = 81/171 (47%), Positives = 110/171 (64%), Gaps = 3/171 (1%)
Frame = +1
Query: 193 GRIKCTLIPGDGVGPELVYAVQEVFKAASIPXDFESFFFSEV---NPTLSAPLEDVVNSI 363
G+ TLIPGDG+G E AV E+FK A++P +FE + + N + L + + S+
Sbjct: 19 GKYTVTLIPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNNKSSGDALHEAIQSL 78
Query: 364 AVNKICIKGILTTPDFSHTGELQTLNMXLRXALDLYANVVHVKSLPNVKCXHXDVDCXII 543
NK+ +KGIL TP F G + N+ LR LD+YA++V +K++P K H +VD II
Sbjct: 79 KRNKVGLKGILFTP-FEKGGHT-SFNVALRKELDIYASLVLIKNIPGFKTRHDNVDFAII 136
Query: 544 TEQTEGXYSALEHESVPGVXECLKIITASKSERIAKXAFDYTVKMGRKXVT 696
E TEG YS LEH+SVPGV E LKIIT KS+RIA+ AFD+ ++ GRK VT
Sbjct: 137 RENTEGEYSGLEHQSVPGVVESLKIITEYKSKRIAQFAFDFALQNGRKSVT 187
>SPBC902.05c |idh2|glu2|isocitrate dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 121 bits (292), Expect = 9e-29
Identities = 65/183 (35%), Positives = 102/183 (55%), Gaps = 2/183 (1%)
Frame = +1
Query: 154 APFGALQPRATKEGRIKCTLIPGDGVGPELVYAVQEVFKAASIPXDFESFFFSEV--NPT 327
A G + G T+I GDG+GPE+ +V+ +FKAA +P ++E + N T
Sbjct: 31 AAAGTYEGVKNANGNYTVTMIAGDGIGPEIAQSVERIFKAAKVPIEWERVKVYPILKNGT 90
Query: 328 LSAPLEDVVNSIAVNKICIKGILTTPDFSHTGELQTLNMXLRXALDLYANVVHVKSLPNV 507
+ P +D S+ NK+ +KG L TP G + ++N+ LR L+ANV S+
Sbjct: 91 TTIP-DDAKESVRKNKVALKGPLATP--IGKGHV-SMNLTLRRTFGLFANVRPCVSITGY 146
Query: 508 KCXHXDVDCXIITEQTEGXYSALEHESVPGVXECLKIITASKSERIAKXAFDYTVKMGRK 687
K + +V+ +I E TEG YS +EHE +PGV + +K+IT + SER+ + AF Y + G+
Sbjct: 147 KTPYDNVNTVLIRENTEGEYSGIEHEVIPGVVQSIKLITRAASERVIRYAFQYARQTGKN 206
Query: 688 XVT 696
+T
Sbjct: 207 NIT 209
>SPAC31G5.04 |||homoisocitrate dehydrogenase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 362
Score = 47.2 bits (107), Expect = 2e-06
Identities = 51/169 (30%), Positives = 77/169 (45%), Gaps = 13/169 (7%)
Frame = +1
Query: 196 RIKCTLIPGDGVGPELVYAVQEVFKAASIPXDFE-SFFFSEVNP-------TLSAPLEDV 351
RI LIP DG+G E+V A + + + ++P + F F +++ T A E
Sbjct: 6 RIVLGLIPADGIGKEVVPAARRLME--NLPAKHKLKFDFIDLDAGWGTFERTGKALPERT 63
Query: 352 VNSIAVN-KICIKGILTTPDFSHTGELQTLNMXLRXALDLYANVVHVKSLPNVKCXHXDV 528
V + + G + +P G + + LR + LYANV VKSL K V
Sbjct: 64 VERLKTECNAALFGAVQSPTHKVAGYSSPI-VALRKKMGLYANVRPVKSLDGAK--GKPV 120
Query: 529 DCXIITEQTEGXYSALEH--ESVPG--VXECLKIITASKSERIAKXAFD 663
D I+ E TE Y E ++ PG V E ++ I+ S +I K AF+
Sbjct: 121 DLVIVRENTECLYVKEERMVQNTPGKRVAEAIRRISEEASTKIGKMAFE 169
>SPBC1A4.02c |leu1|SPBC1E8.07c|3-isopropylmalate dehydrogenase
Leu1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 371
Score = 29.1 bits (62), Expect = 0.64
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = +1
Query: 202 KCTLIPGDGVGPELVYAVQEVFK 270
K ++PGD +GPE+V + EV K
Sbjct: 5 KIVVLPGDHIGPEIVASALEVLK 27
>SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 685
Score = 28.3 bits (60), Expect = 1.1
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -1
Query: 166 LQMVHNIHFSPLLPNLCVH 110
LQ H++H SPL P CVH
Sbjct: 261 LQGTHSVHGSPLKPEDCVH 279
>SPBC3E7.15c |mug83|SPBC4F6.02c|sphingosine N-acyltransferase
Lac1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = -2
Query: 378 NLVNSDRVYDVFEWCTKCWIHFRKEKGFKVXW 283
N VN+ VY +F WI+ R FK+ W
Sbjct: 269 NYVNTVIVYPIFVIFVFVWIYMRHYLNFKIMW 300
>SPAC19G12.02c |pms1||MutL family mismatch-repair protein
Pms1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 794
Score = 27.9 bits (59), Expect = 1.5
Identities = 25/83 (30%), Positives = 38/83 (45%)
Frame = +1
Query: 235 PELVYAVQEVFKAASIPXDFESFFFSEVNPTLSAPLEDVVNSIAVNKICIKGILTTPDFS 414
P A+Q++ S P D + F ++N +LS +D+V S A+ K K I D S
Sbjct: 533 PSKTAALQKLKFFQSRPLDGLNKFSKKINISLSGVQKDIVRSDALLKFSNK-IGVVHDIS 591
Query: 415 HTGELQTLNMXLRXALDLYANVV 483
+ LN+ + A L VV
Sbjct: 592 DENQEDHLNLTVHKADFLRMRVV 614
>SPAC14C4.01c ||SPAC19D5.08c|DUF1770 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 244
Score = 26.2 bits (55), Expect = 4.5
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +1
Query: 550 QTEGXYSALEHESVPGVXECLKIITASKSERIAKXAFDYTV 672
+TE SA+E +P + E +I+ S S A +YTV
Sbjct: 121 ETEDSESAVEEAELPTIFEGKTVISPSSSGTDHSEAVEYTV 161
>SPBP19A11.04c |mor2|cps12|morphogenesis protein
Mor2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2196
Score = 25.8 bits (54), Expect = 6.0
Identities = 14/44 (31%), Positives = 25/44 (56%)
Frame = -2
Query: 192 FLCCPRLQGSKWCITYIFLRCYRTCVYTLSNMLRSLHNSSKNIP 61
FL P +Q + Y+ LRCY + + ++++LH SS+ +P
Sbjct: 1000 FLSTPNVQADE---KYLKLRCYFSQLLEKVLLVQNLHPSSEVLP 1040
>SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor
Spt6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1365
Score = 25.4 bits (53), Expect = 7.9
Identities = 11/37 (29%), Positives = 23/37 (62%), Gaps = 1/37 (2%)
Frame = -2
Query: 381 TNLVNSDRVYDVFEWCTKCW-IHFRKEKGFKVXWNAG 274
T L++ + ++++F CTK W +H +K+ K+ + G
Sbjct: 355 TPLLSQNDLWNIFFLCTKFWSLHSKKQDILKLYSDLG 391
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,701,134
Number of Sequences: 5004
Number of extensions: 55557
Number of successful extensions: 136
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 129
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 131
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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