BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0420
(750 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g02710.1 68416.m00262 nuclear associated protein-related / NA... 112 2e-25
At3g51070.1 68416.m05592 dehydration-responsive protein-related ... 34 0.088
At1g79000.1 68414.m09212 p300/CBP acetyltransferase-related prot... 32 0.47
At2g46400.1 68415.m05775 WRKY family transcription factor 30 1.4
At1g32750.1 68414.m04038 HAC13 protein (HAC13) identical to HAC1... 30 1.9
At5g15070.1 68418.m01766 expressed protein 29 2.5
At4g33500.1 68417.m04758 protein phosphatase 2C-related / PP2C-r... 29 2.5
At3g54540.1 68416.m06035 ABC transporter family protein similar ... 29 2.5
At5g63550.1 68418.m07976 expressed protein 29 3.3
At5g10660.1 68418.m01234 calmodulin-binding protein-related cont... 29 3.3
At4g34390.1 68417.m04885 extra-large guanine nucleotide binding ... 29 3.3
At3g55450.1 68416.m06158 protein kinase, putative similar to pro... 29 3.3
At2g39660.1 68415.m04864 protein kinase, putative similar to pro... 29 3.3
At5g33230.1 68418.m03927 hypothetical protein 29 4.4
At3g04820.1 68416.m00522 expressed protein contains PFam profile... 29 4.4
At1g24560.1 68414.m03090 expressed protein 29 4.4
At3g18610.1 68416.m02365 nucleolin, putative contains Pfam profi... 28 5.8
At3g01310.1 68416.m00042 expressed protein similar to unknown pr... 28 5.8
At1g53800.1 68414.m06123 expressed protein 28 5.8
At1g03320.1 68414.m00311 hypothetical protein 28 5.8
At5g60030.1 68418.m07527 expressed protein 28 7.6
At2g41870.1 68415.m05177 remorin family protein contains Pfam do... 28 7.6
At1g63980.1 68414.m07247 D111/G-patch domain-containing protein ... 28 7.6
>At3g02710.1 68416.m00262 nuclear associated protein-related /
NAP-related similar to Nuclear associated protein (NAP)
(NYD-SP19) (Swiss-Prot:Q8WYA6) [Homo sapiens]
Length = 529
Score = 112 bits (270), Expect = 2e-25
Identities = 58/137 (42%), Positives = 80/137 (58%)
Frame = +1
Query: 340 EVLDETTVKKLVLNFEKKALRNREMRIKFPDQPDKFMDSEIDLHDALQDLSAVATVPDQY 519
E LD T+KKLVL+FE++ N R+K+ + P+KF DSE+DLHD LQ L +A P+ Y
Sbjct: 47 EALDLKTLKKLVLSFERRLRDNIAARLKYVENPEKFADSEVDLHDDLQKLKVLAGAPELY 106
Query: 520 PLLVELKCVNSLLELLSHDNTDVSTKVVQLLQEMTDVDILHESEEXAEDXXXXXXXXXXX 699
P LV V S++ LLSH+N+D++ VVQLLQ++TD D L +++E A
Sbjct: 107 PDLVASNTVPSIVNLLSHENSDIANDVVQLLQDLTDEDALEDNDEPARVLVDALVENNVL 166
Query: 700 XXXXXXXTRLDXQVPDE 750
RL PDE
Sbjct: 167 ELLVQNMNRLSEADPDE 183
>At3g51070.1 68416.m05592 dehydration-responsive protein-related
similar to early-responsive to dehydration stress ERD3
protein [Arabidopsis thaliana] GI:15320410; contains
Pfam profile PF03141: Putative methyltransferase
Length = 895
Score = 34.3 bits (75), Expect = 0.088
Identities = 34/108 (31%), Positives = 54/108 (50%), Gaps = 1/108 (0%)
Frame = +1
Query: 91 PFRLKQKMDVAELLTFKPTPTPKRPNEDESDEEDQAKSAKIR-RTTKLKQEPVHLSKNML 267
P KQ+ D E F+ P K P++ E++Q KSAK + TT K + +N
Sbjct: 74 PEPTKQESD--EQQAFEDNPG-KLPDDAVKSEDEQRKSAKEKSETTSSKTQTQETQQN-- 128
Query: 268 KEPSITDKEREDILKFVATEVTEGEVLDETTVKKLVLNFEKKALRNRE 411
+ I++++ +D K T V E E E +KK+V FEK+ + R+
Sbjct: 129 NDDKISEEKEKDNGKENQT-VQESE---EGQMKKVVKEFEKEQKQQRD 172
>At1g79000.1 68414.m09212 p300/CBP acetyltransferase-related protein 2
(PCAT2) contains Pfam domains PF02135: TAZ zinc finger
and PF00569: Zinc finger, ZZ type; identical to cDNA
p300/CBP acetyltransferase-related protein 2 GI:12597460
Length = 1691
Score = 31.9 bits (69), Expect = 0.47
Identities = 25/91 (27%), Positives = 49/91 (53%), Gaps = 4/91 (4%)
Frame = +1
Query: 154 PKRPNEDESDEEDQAKSAKIRRTTKLKQEPVHLSK-NMLKEPSITDKEREDILKFVA-TE 327
PK+ + D++D + + ++ TTK+++EP L K N+ + T K + +K V+ TE
Sbjct: 844 PKQRPVSQPDKQDLSDVSPMQETTKVEKEPESLKKENLAESTEHTSKSGKPEIKGVSLTE 903
Query: 328 VTEGEVLDE--TTVKKLVLNFEKKALRNREM 414
+ E + E +++ V + KA +N+ M
Sbjct: 904 LFTPEQVREHIRGLRQWVGQSKAKAEKNQAM 934
>At2g46400.1 68415.m05775 WRKY family transcription factor
Length = 295
Score = 30.3 bits (65), Expect = 1.4
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +1
Query: 253 SKNMLKEPSITDKEREDILKFVATEVTEGEVLD 351
SKN+ K+ +++K E + FVATE G + D
Sbjct: 73 SKNVFKKRKVSEKNTEKVKVFVATEQENGSIDD 105
>At1g32750.1 68414.m04038 HAC13 protein (HAC13) identical to HAC13
[Arabidopsis thaliana] gi|21105767|gb|AAM34782; contains
Pfam domains, PF00439: Bromodomain and PF00240:
Ubiquitin family
Length = 1919
Score = 29.9 bits (64), Expect = 1.9
Identities = 25/71 (35%), Positives = 37/71 (52%), Gaps = 8/71 (11%)
Frame = +1
Query: 166 NEDESDEEDQAKSAKIRRTTKLKQEPVHLSKNML----KEPSITDKE----REDILKFVA 321
+E+E EE+QA K T K+EPV L ++ +E SI DKE ED+ +
Sbjct: 148 DEEEEQEEEQAPVEKSLETE--KREPVVLKEDKALEYEEEASILDKEDHMDTEDVQEEEV 205
Query: 322 TEVTEGEVLDE 354
E+ EG + D+
Sbjct: 206 DELLEGTLDDK 216
>At5g15070.1 68418.m01766 expressed protein
Length = 1049
Score = 29.5 bits (63), Expect = 2.5
Identities = 26/95 (27%), Positives = 45/95 (47%), Gaps = 1/95 (1%)
Frame = +1
Query: 97 RLKQKMDVAELLTFKPTPTPK-RPNEDESDEEDQAKSAKIRRTTKLKQEPVHLSKNMLKE 273
+LK + + +LL P+ R E +SD ED + K+R+ + +E H S
Sbjct: 407 KLKTAVQLQDLLDATRMLIPRARSGESDSDAEDLEHADKLRQVKAVLEEGGHFS------ 460
Query: 274 PSITDKEREDILKFVATEVTEGEVLDETTVKKLVL 378
I K + LK+V ++GE +E V+ L++
Sbjct: 461 -GIYRKVQLKPLKWVNVPKSDGEGEEERPVEALMV 494
>At4g33500.1 68417.m04758 protein phosphatase 2C-related /
PP2C-related YHR077c (NMD2,IFS1) protein -Saccharomyces
cerevisiae,PID:g555939
Length = 724
Score = 29.5 bits (63), Expect = 2.5
Identities = 21/84 (25%), Positives = 36/84 (42%)
Frame = +1
Query: 160 RPNEDESDEEDQAKSAKIRRTTKLKQEPVHLSKNMLKEPSITDKEREDILKFVATEVTEG 339
RP+ E + +I + ++P+ +N++ EP+ TD D L +E T
Sbjct: 258 RPSLSTQQEITPVSTIEIDDNLDVTEKPIEAEENLVAEPTATDDLSPDEL-LSTSEATHR 316
Query: 340 EVLDETTVKKLVLNFEKKALRNRE 411
V DE K ++ E+ L E
Sbjct: 317 SV-DEIAQKPVIDTSEENLLNTFE 339
>At3g54540.1 68416.m06035 ABC transporter family protein similar to
ABC50 GI:10863747 from [Rattus norvegicus]
Length = 723
Score = 29.5 bits (63), Expect = 2.5
Identities = 20/51 (39%), Positives = 28/51 (54%)
Frame = +1
Query: 148 PTPKRPNEDESDEEDQAKSAKIRRTTKLKQEPVHLSKNMLKEPSITDKERE 300
P ++ ESDEE++ K A+ KLK E HL E S+TDKE++
Sbjct: 71 PPSDEEDDGESDEEERQKEAR----RKLKSEQRHL------EISVTDKEQK 111
>At5g63550.1 68418.m07976 expressed protein
Length = 530
Score = 29.1 bits (62), Expect = 3.3
Identities = 18/70 (25%), Positives = 29/70 (41%)
Frame = +1
Query: 103 KQKMDVAELLTFKPTPTPKRPNEDESDEEDQAKSAKIRRTTKLKQEPVHLSKNMLKEPSI 282
KQK+D E K P +P S E+ +A + ++E + + +LKE
Sbjct: 386 KQKVDHVESSKEKSKKQPSKPQAKGSKEKGKATKKGKAKAEPTRKEMLEVVSKILKEVDF 445
Query: 283 TDKEREDILK 312
DIL+
Sbjct: 446 NTATLSDILQ 455
>At5g10660.1 68418.m01234 calmodulin-binding protein-related
contains weak similarity to calmodulin-binding proteins
Length = 407
Score = 29.1 bits (62), Expect = 3.3
Identities = 16/62 (25%), Positives = 30/62 (48%), Gaps = 3/62 (4%)
Frame = +1
Query: 178 SDEEDQAKSAKIRRTTKLK---QEPVHLSKNMLKEPSITDKEREDILKFVATEVTEGEVL 348
S ED+ + K+ + +EP K+ +P + +E+E +TE +GE++
Sbjct: 201 SAHEDEEEIVKVETDVHISDHGEEPKEEDKDQFAQPDESGEEKETSPVAASTEEQKGELI 260
Query: 349 DE 354
DE
Sbjct: 261 DE 262
>At4g34390.1 68417.m04885 extra-large guanine nucleotide binding
protein, putative / G-protein, putative similar to
extra-large G-protein (XLG) [Arabidopsis thaliana]
GI:3201680; contains Pfam profile PF00503: G-protein
alpha subunit
Length = 861
Score = 29.1 bits (62), Expect = 3.3
Identities = 14/29 (48%), Positives = 20/29 (68%)
Frame = +1
Query: 118 VAELLTFKPTPTPKRPNEDESDEEDQAKS 204
+ +LL F P+P PKR N ESD++D+ S
Sbjct: 5 IRKLLPF-PSPNPKRDNR-ESDDDDETSS 31
>At3g55450.1 68416.m06158 protein kinase, putative similar to
protein kinase APK1B [Arabidopsis thaliana]
SWISS-PROT:P46573
Length = 389
Score = 29.1 bits (62), Expect = 3.3
Identities = 14/25 (56%), Positives = 15/25 (60%)
Frame = +1
Query: 331 TEGEVLDETTVKKLVLNFEKKALRN 405
TEGE+L TTVK N K A RN
Sbjct: 36 TEGEILSSTTVKSFSFNELKLATRN 60
>At2g39660.1 68415.m04864 protein kinase, putative similar to
protein kinase gi|166809|gb|AAA18853
Length = 395
Score = 29.1 bits (62), Expect = 3.3
Identities = 14/29 (48%), Positives = 16/29 (55%)
Frame = +1
Query: 319 ATEVTEGEVLDETTVKKLVLNFEKKALRN 405
A + TEGE+L T VK N K A RN
Sbjct: 38 AAQKTEGEILSSTPVKSFTFNELKLATRN 66
>At5g33230.1 68418.m03927 hypothetical protein
Length = 126
Score = 28.7 bits (61), Expect = 4.4
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = +1
Query: 142 PTPTPKRPNEDESDEEDQAKSAKIRRTTKLKQEPV 246
PT DESDEED+ K K ++ +K K++ V
Sbjct: 57 PTIESLSDGSDESDEEDKKKKKKKKQISKKKRKHV 91
>At3g04820.1 68416.m00522 expressed protein contains PFam profile
PF01142: Uncharacterized protein family UPF0024;
expression supported by MPSS
Length = 747
Score = 28.7 bits (61), Expect = 4.4
Identities = 22/62 (35%), Positives = 29/62 (46%)
Frame = +1
Query: 163 PNEDESDEEDQAKSAKIRRTTKLKQEPVHLSKNMLKEPSITDKEREDILKFVATEVTEGE 342
P E EED+AKS + Q ++ S+TD ER +IL T T+GE
Sbjct: 88 PPEMAVKEEDKAKSCD-----NVPQNYTSAVESFKSISSVTDAERLEILINQVTSGTDGE 142
Query: 343 VL 348
VL
Sbjct: 143 VL 144
>At1g24560.1 68414.m03090 expressed protein
Length = 678
Score = 28.7 bits (61), Expect = 4.4
Identities = 33/162 (20%), Positives = 70/162 (43%), Gaps = 12/162 (7%)
Frame = +1
Query: 217 RTTKLKQEPVHLSKNMLKEPSITDKEREDILKFVATEVTEGEVLDETTVKKLVLNFEKKA 396
+ L +E H KN+ + + KE+E I + + +++ + ++ + K+ + +
Sbjct: 361 KAQSLVEEKSHELKNLNETVGLLVKEKEHIGTLLRSALSKRVIGEQPSQKRELFQAAENG 420
Query: 397 LRNREMRIKFPD--QPDKFMDSEID-LHDALQDLSAVATVPDQYPLLV-----ELKCVNS 552
LR+ KF + K DS D HD ++ + + ++ +V E+ +
Sbjct: 421 LRDGGTDSKFAKLLKDGKVQDSRSDNTHDHSKEDNEIYSLASTLENIVKASQLEIVELQH 480
Query: 553 LLELLSHDNT----DVSTKVVQLLQEMTDVDILHESEEXAED 666
LLE + + + T+ +L Q M ++ L E E A +
Sbjct: 481 LLEASREETSSLRKQLDTQTKELNQRMRQIEELKEKERIANE 522
>At3g18610.1 68416.m02365 nucleolin, putative contains Pfam profile:
PF00076 RNA recognition motif
Length = 636
Score = 28.3 bits (60), Expect = 5.8
Identities = 15/52 (28%), Positives = 28/52 (53%)
Frame = +1
Query: 139 KPTPTPKRPNEDESDEEDQAKSAKIRRTTKLKQEPVHLSKNMLKEPSITDKE 294
+PTP K P + D D++ S + T +K++P + K+ E S +++E
Sbjct: 222 EPTPAKKEPIVVKKDSSDESSSDE--ETPVVKKKPTTVVKDAKAESSSSEEE 271
>At3g01310.1 68416.m00042 expressed protein similar to unknown
protein GB:BAA24863 [Homo sapiens], unknown protein
GB:BAA20831 [Homo sapiens], unknown protein GB:AAB42264
[Caenorhabditis elegans]
Length = 1056
Score = 28.3 bits (60), Expect = 5.8
Identities = 26/96 (27%), Positives = 46/96 (47%), Gaps = 2/96 (2%)
Frame = +1
Query: 97 RLKQKMDVAELLTFKPTPTPK-RPN-EDESDEEDQAKSAKIRRTTKLKQEPVHLSKNMLK 270
+LK + + +LL P+ RP E +SD ED + K+R+ + +E H S
Sbjct: 414 KLKSAVQLQDLLDATRMLVPRTRPGRESDSDAEDLEHAEKLRQVKAVLEEGGHFS----- 468
Query: 271 EPSITDKEREDILKFVATEVTEGEVLDETTVKKLVL 378
I K + LK+V ++G+ +E V+ L++
Sbjct: 469 --GIYRKVQLKPLKWVKIPKSDGDGEEERPVEALMV 502
>At1g53800.1 68414.m06123 expressed protein
Length = 568
Score = 28.3 bits (60), Expect = 5.8
Identities = 16/46 (34%), Positives = 25/46 (54%), Gaps = 2/46 (4%)
Frame = +1
Query: 139 KPTPTPKRPNEDESDEEDQAKSAKIR-RTTKLKQEPVHLSK-NMLK 270
K TPT K + E + + Q + K+R R T ++P+ SK M+K
Sbjct: 302 KKTPTKKSTRDSEFERQSQVQVVKVRKRKTPAYKDPLASSKLEMIK 347
>At1g03320.1 68414.m00311 hypothetical protein
Length = 220
Score = 28.3 bits (60), Expect = 5.8
Identities = 17/61 (27%), Positives = 30/61 (49%)
Frame = +1
Query: 160 RPNEDESDEEDQAKSAKIRRTTKLKQEPVHLSKNMLKEPSITDKEREDILKFVATEVTEG 339
+ NEDE DEED + K+ + K++ N K DK+ +++L++ T+
Sbjct: 20 KKNEDEPDEEDVKQKQKVSKNENPKKD-----SNGEKGSDEKDKKNKNVLRWPQDSSTKK 74
Query: 340 E 342
E
Sbjct: 75 E 75
>At5g60030.1 68418.m07527 expressed protein
Length = 292
Score = 27.9 bits (59), Expect = 7.6
Identities = 19/59 (32%), Positives = 30/59 (50%), Gaps = 4/59 (6%)
Frame = +1
Query: 82 QKLPFRLKQKMDVAELLTFKPTPTPKRPNEDESDE----EDQAKSAKIRRTTKLKQEPV 246
+K+ +L+ + A+ K + K +ED DE ED+ KSA+I+ K K E V
Sbjct: 167 EKVKEKLEDEQKSADRKERKKKKSKKNNDEDVVDEKEKLEDEQKSAEIKEKKKNKDEDV 225
>At2g41870.1 68415.m05177 remorin family protein contains Pfam
domain, PF03763: Remorin, C-terminal region
Length = 274
Score = 27.9 bits (59), Expect = 7.6
Identities = 20/72 (27%), Positives = 35/72 (48%), Gaps = 4/72 (5%)
Frame = +1
Query: 319 ATEVTEGEVLDETTVKKLVLNFEKKALRNREMRIKFPDQ-PDKFMDSEIDLH---DALQD 486
AT ++EGE + + L ++ N E+ + Q D+ D + +H D ++
Sbjct: 63 ATTMSEGENFTTISREFNALVIAGSSMENNELMTRDVTQREDERQDELMRIHEDTDHEEE 122
Query: 487 LSAVATVPDQYP 522
+ +A VPDQYP
Sbjct: 123 TNPLAIVPDQYP 134
>At1g63980.1 68414.m07247 D111/G-patch domain-containing protein
contains Pfam profile PF01585: G-patch domain
Length = 391
Score = 27.9 bits (59), Expect = 7.6
Identities = 14/48 (29%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
Frame = +1
Query: 94 FRLKQKMDVAELLTFKPTPTPKRPNEDESDEEDQAKSAKIR-RTTKLK 234
F Q ++ + L + PT N+D+SD+ED+++ ++ KLK
Sbjct: 66 FDTTQFDNILKKLKVQAAPTKTSKNDDDSDKEDESEDDAVKSEPAKLK 113
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,361,719
Number of Sequences: 28952
Number of extensions: 277091
Number of successful extensions: 1185
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 1110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1182
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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