Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0419
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1751.03 ||SPAC31A2.01|translation initiation factor eIF3m|Sc... 50 5e-07
SPBC16A3.13 |meu7|aah4|alpha-amylase homolog Aah4|Schizosaccharo... 32 0.10
SPAC25G10.08 |||translation initiation factor eIF3b |Schizosacch... 29 0.94
SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces p... 29 0.94
SPAC1B3.02c |||transcription elongation factor, Elf1 family|Schi... 27 2.2
SPAC6F6.08c |cdc16|bub2|two-component GAP Cdc16|Schizosaccharomy... 27 3.8
SPCC1902.02 |mug72|SPCC663.16c|ketopantoate reductase |Schizosac... 26 6.6
SPAC27F1.04c |nuf2||spindle pole body protein Nuf2|Schizosacchar... 26 6.6
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 26 6.6
SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16 |Schizosa... 25 8.7
SPCC1919.01 |ppk34|SPCC830.12|serine/threonine protein kinase Pp... 25 8.7
>SPAC1751.03 ||SPAC31A2.01|translation initiation factor
eIF3m|Schizosaccharomyces pombe|chr 1|||Manual
Length = 402
Score = 49.6 bits (113), Expect = 5e-07
Identities = 41/174 (23%), Positives = 86/174 (49%), Gaps = 9/174 (5%)
Frame = +1
Query: 64 ACAELIKQVLEAPGVHVFGELLEMPNIKELESGPYATHLKTLNLFAYGTYKDYI----EN 231
A EL+++ + +P F +++ +P +++LE L+ L + + G DY+ EN
Sbjct: 210 AVRELVQRAVNSPKYFFFDDIVTLPPVQQLEQST----LQLLGILSGGMTDDYVSWVAEN 265
Query: 232 KSDYLELTTVQ---CKKLQHLTIATLATQ--EKCIPYSVLLEELDIKNVRDLEDLIIEAI 396
+ + +K++ LTIA+LATQ + Y + + L I + ++E II+ I
Sbjct: 266 HAHCQHQKFDEDAIARKMKLLTIASLATQAPNNTLSYGDVAKSLKI-DENEVELWIIDVI 324
Query: 397 YADIIHGKLDQECKRVEVDVALGRXARLEDAASIADVLADWCNACEAVLNSVDQ 558
A ++ G++ Q K + + + R + ++ + LA W ++ +L ++Q
Sbjct: 325 RAGLVEGRMSQLTKTLSIHRSSYRVFGKHEWVALHEKLAKWGSSLRYMLQVMEQ 378
>SPBC16A3.13 |meu7|aah4|alpha-amylase homolog
Aah4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 774
Score = 31.9 bits (69), Expect = 0.10
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = -3
Query: 526 HCTSRPGHQQWKLHLQAAHXCQGPRPLPH 440
H ++P H+ WK H + H + PRP+PH
Sbjct: 381 HNVTKPDHKPWK-HEEHCHHGKFPRPVPH 408
Score = 31.5 bits (68), Expect = 0.13
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = -3
Query: 526 HCTSRPGHQQWKLHLQAAHXCQGPRPLPH 440
H ++P H+ WK H + H + PRP+PH
Sbjct: 271 HNGTKPDHKPWK-HEEHCHHGKFPRPIPH 298
Score = 31.1 bits (67), Expect = 0.18
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = -3
Query: 526 HCTSRPGHQQWKLHLQAAHXCQGPRPLPH 440
H ++P H+ WK H + H + PRP+PH
Sbjct: 186 HNGTKPDHKPWK-HEEHCHHGKFPRPVPH 213
Score = 31.1 bits (67), Expect = 0.18
Identities = 12/29 (41%), Positives = 18/29 (62%)
Frame = -3
Query: 526 HCTSRPGHQQWKLHLQAAHXCQGPRPLPH 440
H ++P H+ WK H + H + PRP+PH
Sbjct: 298 HNGTKPDHKPWK-HEEHCHHGRFPRPVPH 325
>SPAC25G10.08 |||translation initiation factor eIF3b
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 725
Score = 28.7 bits (61), Expect = 0.94
Identities = 25/84 (29%), Positives = 40/84 (47%), Gaps = 3/84 (3%)
Frame = +1
Query: 121 ELLEMPNIKELESGPYATHLKTLNLFAYGTYKDYIENKSDYLELTTVQCKKLQHLTIATL 300
E+ P L S P + ++T NLF K Y ++ DYL + + K + T + L
Sbjct: 379 EITNQPARVALISIPSKSTIRTKNLFNVSDCKLYWQSNGDYLCVKVDRHTKTKKSTFSNL 438
Query: 301 ---ATQEKCIPYSVLLEELDIKNV 363
+EK IP +E +D+K+V
Sbjct: 439 EIFRIREKNIP----VEVVDLKDV 458
>SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 387
Score = 28.7 bits (61), Expect = 0.94
Identities = 18/63 (28%), Positives = 27/63 (42%), Gaps = 1/63 (1%)
Frame = +1
Query: 166 YATHLKTLNLFAYGTYKDYIENKSDYLELTTVQCKKLQHLTIATLATQEKCI-PYSVLLE 342
YA + YK Y+E +SDY EL K+L + + + CI S L+
Sbjct: 161 YAKSAHSTKTVVDALYKSYLEKQSDYSELLDNYKKQLNKASESCKGNYQACIESRSTLIT 220
Query: 343 ELD 351
L+
Sbjct: 221 SLE 223
>SPAC1B3.02c |||transcription elongation factor, Elf1
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 107
Score = 27.5 bits (58), Expect = 2.2
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1
Query: 502 DVLADWCNACEAVLNSVDQHIQRANHQK 585
DV +DW +AC+AV N + + N Q+
Sbjct: 68 DVYSDWIDACDAVANQA-KEVDNINDQE 94
>SPAC6F6.08c |cdc16|bub2|two-component GAP Cdc16|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 299
Score = 26.6 bits (56), Expect = 3.8
Identities = 12/37 (32%), Positives = 22/37 (59%), Gaps = 3/37 (8%)
Frame = +1
Query: 250 LTTVQCKKLQHLTIATLATQEKCIP---YSVLLEELD 351
+ ++ CK+LQ L + Q KCI Y V+L++++
Sbjct: 1 MPSIDCKRLQKLAPKSPENQSKCISRLRYMVMLDQVE 37
>SPCC1902.02 |mug72|SPCC663.16c|ketopantoate reductase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 574
Score = 25.8 bits (54), Expect = 6.6
Identities = 15/33 (45%), Positives = 21/33 (63%), Gaps = 1/33 (3%)
Frame = -3
Query: 712 WVLSEARHWHXTLIHHSLLESSMS-FLXIKSSV 617
W LSE++ H TLI + ES +S + I+SSV
Sbjct: 25 WRLSESKACHTTLIWRNRCESVLSEGIRIRSSV 57
>SPAC27F1.04c |nuf2||spindle pole body protein
Nuf2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 441
Score = 25.8 bits (54), Expect = 6.6
Identities = 13/46 (28%), Positives = 23/46 (50%)
Frame = +1
Query: 244 LELTTVQCKKLQHLTIATLATQEKCIPYSVLLEELDIKNVRDLEDL 381
LE V+ KL+H T+ QE C + ++L+ + + L+ L
Sbjct: 295 LEECLVELDKLEHATVLLSTNQELCDQIEINKKKLEFRKEQLLKQL 340
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 25.8 bits (54), Expect = 6.6
Identities = 8/26 (30%), Positives = 13/26 (50%)
Frame = +1
Query: 481 EDAASIADVLADWCNACEAVLNSVDQ 558
+D + D A WC C+A+ +Q
Sbjct: 17 QDKLVVVDFFATWCGPCKAIAPKFEQ 42
>SPCC4G3.19 |alp16||gamma tubulin complex subunit Alp16
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 759
Score = 25.4 bits (53), Expect = 8.7
Identities = 11/27 (40%), Positives = 19/27 (70%)
Frame = -1
Query: 228 LNVVLICAIGKQIKSF*MSCIRSTLQL 148
LN+ + C I + I+SF +S +RST ++
Sbjct: 491 LNLNVCCCIAEPIQSFILSFLRSTYKV 517
>SPCC1919.01 |ppk34|SPCC830.12|serine/threonine protein kinase
Ppk34|Schizosaccharomyces pombe|chr 3|||Manual
Length = 354
Score = 25.4 bits (53), Expect = 8.7
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +1
Query: 298 LATQEKCIPYSVLLEELDIKNVRDLEDLI 384
+ QEK PY + +ELD+ V D E+++
Sbjct: 106 IRNQEKKDPYYYIRKELDVLKVLDHENVV 134
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,792,369
Number of Sequences: 5004
Number of extensions: 52356
Number of successful extensions: 159
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 152
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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