BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0415
(800 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO) ... 93 1e-19
At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical... 93 3e-19
At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO) ... 76 2e-14
At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO) ... 61 7e-10
At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351 ... 57 1e-08
At5g48700.1 68418.m06027 ubiquitin-related contains similarity t... 52 6e-07
At5g55855.1 68418.m06962 hypothetical protein 45 7e-05
At1g68185.1 68414.m07789 ubiquitin-related similar to ubiquitin-... 43 3e-04
>At5g55160.1 68418.m06877 small ubiquitin-like modifier 2 (SUMO)
similar to ubiquitin-like protein SMT3 SP:P55852 from
[Arabidopsis thaliana]; identical to cDNA small
ubiquitin-like modifier 2 (SUMO) GI:22652843; contains
Pfam profile PF00240: Ubiquitin family
Length = 103
Score = 93.5 bits (222), Expect = 1e-19
Identities = 43/85 (50%), Positives = 54/85 (63%)
Frame = +1
Query: 100 DEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQPIN 279
D+K + HINLKV GQD V F+IK+ T L+KLMNAYCDR + + F FDG+ +
Sbjct: 8 DKKPDQGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDFNSIAFLFDGRRLR 67
Query: 280 ENDTPTSLEMEEGDTIEVYQQQTGG 354
TP LEME+GD I+ QTGG
Sbjct: 68 AEQTPDELEMEDGDEIDAMLHQTGG 92
>At4g26840.1 68417.m03864 ubiquitin-like protein (SMT3) identical to
Ubiquitin-like protein SMT3 SP:P55852 from[Arabidopsis
thaliana]; identical to cDNA SMT3 protein GI:1707371
Length = 100
Score = 92.7 bits (220), Expect = 3e-19
Identities = 44/86 (51%), Positives = 56/86 (65%), Gaps = 1/86 (1%)
Frame = +1
Query: 100 DEKKGEN-EHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQPI 276
D+K G+ HINLKV GQD V F+IK+ T L+KLMNAYCDR + M + F FDG+ +
Sbjct: 8 DKKPGDGGAHINLKVKGQDGNEVFFRIKRSTQLKKLMNAYCDRQSVDMNSIAFLFDGRRL 67
Query: 277 NENDTPTSLEMEEGDTIEVYQQQTGG 354
TP L+ME+GD I+ QTGG
Sbjct: 68 RAEQTPDELDMEDGDEIDAMLHQTGG 93
>At5g55170.1 68418.m06878 small ubiquitin-like modifier 3 (SUMO)
similar to SP|O13351 Ubiquitin-like protein smt3/pmt3
{Schizosaccharomyces pombe}; identical to cDNA small
ubiquitin-like modifier 3 (SUMO) GI:22652845
Length = 111
Score = 76.2 bits (179), Expect = 2e-14
Identities = 36/81 (44%), Positives = 48/81 (59%)
Frame = +1
Query: 115 ENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRFDGQPINENDTP 294
+ H+ LKV QD V FK KK PL+KLM YCDR GL + F F+G I +TP
Sbjct: 14 QEAHVILKVKSQDGDEVLFKNKKSAPLKKLMYVYCDRRGLKLDAFAFIFNGARIGGLETP 73
Query: 295 TSLEMEEGDTIEVYQQQTGGV 357
L+ME+GD I+ + +GG+
Sbjct: 74 DELDMEDGDVIDACRAMSGGL 94
>At2g32765.1 68415.m04009 small ubiquitin-like modifier 5 (SUMO)
similar to ubiquitin-like protein SMT3 SP:P55852
[Arabidopsis thaliana]; contains INTERPRO:IPR000626
ubiquitin domain; contains Pfam profile PF00240:
Ubiquitin family; contains Pfam profile PF00240:
Ubiquitin family; identical to cDNA small
ubiquitin-like modifier 5 (SUMO) mRNA GI:22652847
Length = 108
Score = 61.3 bits (142), Expect = 7e-10
Identities = 32/91 (35%), Positives = 46/91 (50%)
Frame = +1
Query: 82 INLKMADEKKGENEHINLKVLGQDNAIVQFKIKKHTPLRKLMNAYCDRAGLSMQVVRFRF 261
++ K + + + LKV Q A +KI H L+KLM+AYC + L VRF +
Sbjct: 13 VSKKSRSPETSPHMKVTLKVKNQQGAEDLYKIGTHAHLKKLMSAYCTKRNLDYSSVRFVY 72
Query: 262 DGQPINENDTPTSLEMEEGDTIEVYQQQTGG 354
+G+ I TP L MEE D I + + GG
Sbjct: 73 NGREIKARQTPAQLHMEEEDEICMVMELGGG 103
>At5g48710.1 68418.m06029 ubiquitin-related similar to SP|O13351
Ubiquitin-like protein smt3/pmt3 {Schizosaccharomyces
pombe}
Length = 114
Score = 57.2 bits (132), Expect = 1e-08
Identities = 32/93 (34%), Positives = 47/93 (50%), Gaps = 4/93 (4%)
Frame = +1
Query: 88 LKMADEKK---GENEHINLKVLGQDNAIVQ-FKIKKHTPLRKLMNAYCDRAGLSMQVVRF 255
+KM +K+ + H+ LKV GQD + F ++++ L K+M Y G+ RF
Sbjct: 12 VKMEGQKRKVVSDPTHVTLKVKGQDEEDFRVFWVRRNAKLLKMMELYTKMRGIEWNTFRF 71
Query: 256 RFDGQPINENDTPTSLEMEEGDTIEVYQQQTGG 354
FDG I E TP LE ++GD I+ Q G
Sbjct: 72 LFDGSRIREYHTPDELERKDGDEIDAMLCQQSG 104
>At5g48700.1 68418.m06027 ubiquitin-related contains similarity to
SP|O13351 Ubiquitin-like protein smt3/pmt3
{Schizosaccharomyces pombe}
Length = 117
Score = 51.6 bits (118), Expect = 6e-07
Identities = 32/95 (33%), Positives = 47/95 (49%), Gaps = 6/95 (6%)
Frame = +1
Query: 88 LKMADEKK----GENEHINLKVLGQDNAIVQ-FKIKKHTPLRKLMNAYCDRAGLSMQVVR 252
+KM EK+ E+ H+ L V GQD V+ F++++ L KLM Y G+ R
Sbjct: 14 VKMEGEKRKDVESESTHVTLNVKGQDEEGVKVFRVRRKARLLKLMEYYAKMRGIEWNTFR 73
Query: 253 FRFD-GQPINENDTPTSLEMEEGDTIEVYQQQTGG 354
F D G I E T +E+++GD I+ Q G
Sbjct: 74 FLSDDGSRIREYHTADDMELKDGDQIDALLPQESG 108
>At5g55855.1 68418.m06962 hypothetical protein
Length = 59
Score = 44.8 bits (101), Expect = 7e-05
Identities = 22/56 (39%), Positives = 29/56 (51%), Gaps = 3/56 (5%)
Frame = +1
Query: 202 LMNAYCDRAGLSMQVVRFRFDGQPINENDTPT---SLEMEEGDTIEVYQQQTGGVS 360
+M AY D+ G M RF DG I N TP L++E+GD I+ + Q G S
Sbjct: 1 MMQAYSDKVGQQMSAFRFHCDGIRIKPNQTPNEELQLDLEDGDEIDAFVDQIAGFS 56
>At1g68185.1 68414.m07789 ubiquitin-related similar to
ubiquitin-like protein smt3/pmt3 SP:O13351 from [Fission
yeast]
Length = 215
Score = 42.7 bits (96), Expect = 3e-04
Identities = 20/50 (40%), Positives = 32/50 (64%)
Frame = +1
Query: 199 KLMNAYCDRAGLSMQVVRFRFDGQPINENDTPTSLEMEEGDTIEVYQQQT 348
+++ Y D+A L Q + F FDG I+ + TP+ L ME+ D IEV+ ++T
Sbjct: 166 RVIKLYTDKAKLDPQNLVFIFDGDKIDPSTTPSELGMEDHDMIEVHTKKT 215
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,906,824
Number of Sequences: 28952
Number of extensions: 277288
Number of successful extensions: 568
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 556
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 568
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1814318400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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