BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0413 (600 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 26 3.7 SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ... 25 6.4 SPAC222.03c |tim10||Tim9-Tim10 complex subunit Tim10|Schizosacch... 25 8.5 SPBC2G2.14 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 8.5 SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharo... 25 8.5 >SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizosaccharomyces pombe|chr 1|||Manual Length = 1290 Score = 26.2 bits (55), Expect = 3.7 Identities = 16/53 (30%), Positives = 27/53 (50%) Frame = +1 Query: 109 NFNLTAYQGIWYEISKFPNESEKNGKCSSAEYKLESDVVKVKNVHIIDGVKKY 267 N N +GI E+SK+ + KN + SS + K V+ + I+G+K + Sbjct: 376 NINEINEEGIMTEVSKYASLVNKNYEISSGKLKERQVAVRAR----IEGIKAH 424 >SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 500 Score = 25.4 bits (53), Expect = 6.4 Identities = 20/78 (25%), Positives = 43/78 (55%), Gaps = 4/78 (5%) Frame = +1 Query: 64 EVIHEXTCPELKPVN-NFNLTAY-QGIWYEISKFPNESE--KNGKCSSAEYKLESDVVKV 231 EV+H+ C +++ + +F + A+ ++Y+ ++F S + CS+ +Y + K Sbjct: 276 EVLHDTRCVKIQEDSESFWIMAHCLKMFYDETEFLPLSGLLPDMNCSTQQYVKLQVIYKE 335 Query: 232 KNVHIIDGVKKYIERTAK 285 K+ + I KKY+++T K Sbjct: 336 KSENDILKFKKYVQQTLK 353 >SPAC222.03c |tim10||Tim9-Tim10 complex subunit Tim10|Schizosaccharomyces pombe|chr 1|||Manual Length = 89 Score = 25.0 bits (52), Expect = 8.5 Identities = 15/41 (36%), Positives = 21/41 (51%) Frame = +1 Query: 184 KCSSAEYKLESDVVKVKNVHIIDGVKKYIERTAKLTDDANK 306 KC S +Y E+D+ K ++V I V KY E L+ K Sbjct: 42 KCISPKY-YEADLTKGESVCIDRCVSKYFEANQSLSQHMQK 81 >SPBC2G2.14 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 533 Score = 25.0 bits (52), Expect = 8.5 Identities = 12/50 (24%), Positives = 29/50 (58%) Frame = +1 Query: 346 SRDGSVQILATDYNNYAIAYNCKYDDKKKSHQVFVWILSRNKKLESDAKT 495 S + +++ ++ D NN A +N +YD+ ++ F ++ R K +E + ++ Sbjct: 161 SLEANLKAISKDSNNKA--HNNRYDESSLTNPEFSILVERLKSIEENLQS 208 >SPCC777.13 |vps35||retromer complex subunit Vps35|Schizosaccharomyces pombe|chr 3|||Manual Length = 785 Score = 25.0 bits (52), Expect = 8.5 Identities = 10/40 (25%), Positives = 22/40 (55%) Frame = +1 Query: 214 SDVVKVKNVHIIDGVKKYIERTAKLTDDANKAAKLTVTFK 333 S + +V NV +ID +K ++ER + ++ + + F+ Sbjct: 746 SSIWEVANVSVIDSLKNHLERATAYAEKRSEDERWSSIFQ 785 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.316 0.131 0.379 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,949,139 Number of Sequences: 5004 Number of extensions: 34585 Number of successful extensions: 101 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 97 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 101 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 262236260 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits)
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