BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0410 (700 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati... 256 1e-68 At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2... 248 3e-66 At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1... 248 4e-66 At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1... 248 4e-66 At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati... 246 8e-66 At2g03270.1 68415.m00280 DNA-binding protein, putative similar t... 28 5.2 At3g43300.1 68416.m04570 guanine nucleotide exchange family prot... 28 6.8 At1g54960.1 68414.m06277 NPK1-related protein kinase, putative (... 28 6.8 At2g45000.1 68415.m05603 expressed protein contains Pfam profile... 27 9.0 At1g05055.1 68414.m00506 basic transcription factor 2, 44kD subu... 27 9.0 >At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative similar to S-adenosylmethionine synthetase 2 (Methionine adenosyltransferase 2, AdoMet synthetase 2) [Catharanthus roseus] SWISS-PROT:Q96552 Length = 393 Score = 256 bits (626), Expect = 1e-68 Identities = 123/196 (62%), Positives = 147/196 (75%), Gaps = 2/196 (1%) Frame = +2 Query: 119 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 298 FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT MV++ GEIT+KANV Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63 Query: 299 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RNDEEVGAGDQ 472 DY+++VR+T + IG+ + G D C V++ ++QQSP+IA GVH + + EEVGAGDQ Sbjct: 64 DYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEVGAGDQ 123 Query: 473 GLMFGYATDETEECMPLTVVLAHKLNQKIAELXRNGEFWWARPDSKTQVTCEYVFAGGAT 652 G MFGYATDET E MPLT VLA KL K+ E+ +NG W RPD KTQVT EY+ GA Sbjct: 124 GHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYINESGAM 183 Query: 653 VPQXVHTVXXSLQHSE 700 VP VHTV S QH E Sbjct: 184 VPVRVHTVLISTQHDE 199 >At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2) identical to S-adenosylmethionine synthetase 2 (Methionine adenosyltransferase 2, AdoMet synthetase 2) [Arabidopsis thaliana] SWISS-PROT:P17562 Length = 393 Score = 248 bits (607), Expect = 3e-66 Identities = 117/196 (59%), Positives = 144/196 (73%), Gaps = 2/196 (1%) Frame = +2 Query: 119 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 298 FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++ GEIT+KA + Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATI 63 Query: 299 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RNDEEVGAGDQ 472 DY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH + + E++GAGDQ Sbjct: 64 DYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQ 123 Query: 473 GLMFGYATDETEECMPLTVVLAHKLNQKIAELXRNGEFWWARPDSKTQVTCEYVFAGGAT 652 G MFGYATDET E MPL+ VLA K+ ++ E+ +NG W RPD KTQVT EY GA Sbjct: 124 GHMFGYATDETPELMPLSHVLATKIGARLTEVRKNGTCRWLRPDGKTQVTVEYYNDNGAM 183 Query: 653 VPQXVHTVXXSLQHSE 700 VP VHTV S QH E Sbjct: 184 VPVRVHTVLISTQHDE 199 >At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1) identical to S-adenosylmethionine synthetase 1 (Methionine adenosyltransferase 1, AdoMet synthetase 1) [Arabidopsis thaliana] SWISS-PROT:P23686 Length = 393 Score = 248 bits (606), Expect = 4e-66 Identities = 120/196 (61%), Positives = 144/196 (73%), Gaps = 2/196 (1%) Frame = +2 Query: 119 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 298 FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++ GEIT+KA V Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63 Query: 299 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RNDEEVGAGDQ 472 DY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH + + EE+GAGDQ Sbjct: 64 DYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKCPEEIGAGDQ 123 Query: 473 GLMFGYATDETEECMPLTVVLAHKLNQKIAELXRNGEFWWARPDSKTQVTCEYVFAGGAT 652 G MFGYATDET E MPL+ VLA KL ++ E+ +NG W RPD KTQVT EY GA Sbjct: 124 GHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGAM 183 Query: 653 VPQXVHTVXXSLQHSE 700 VP VHTV S QH E Sbjct: 184 VPIRVHTVLISTQHDE 199 >At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1) identical to S-adenosylmethionine synthetase 1 (Methionine adenosyltransferase 1, AdoMet synthetase 1) [Arabidopsis thaliana] SWISS-PROT:P23686 Length = 393 Score = 248 bits (606), Expect = 4e-66 Identities = 120/196 (61%), Positives = 144/196 (73%), Gaps = 2/196 (1%) Frame = +2 Query: 119 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 298 FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++ GEIT+KA V Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63 Query: 299 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RNDEEVGAGDQ 472 DY+K+VR+T + IG+ G D C V++ ++QQSP+IA GVH + + EE+GAGDQ Sbjct: 64 DYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKCPEEIGAGDQ 123 Query: 473 GLMFGYATDETEECMPLTVVLAHKLNQKIAELXRNGEFWWARPDSKTQVTCEYVFAGGAT 652 G MFGYATDET E MPL+ VLA KL ++ E+ +NG W RPD KTQVT EY GA Sbjct: 124 GHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGAM 183 Query: 653 VPQXVHTVXXSLQHSE 700 VP VHTV S QH E Sbjct: 184 VPIRVHTVLISTQHDE 199 >At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative similar to S-adenosylmethionine synthetase 3 (Methionine adenosyltransferase 3, AdoMet synthetase 3) [Lycopersicon esculentum] SWISS-PROT:P43282 Length = 390 Score = 246 bits (603), Expect = 8e-66 Identities = 119/196 (60%), Positives = 144/196 (73%), Gaps = 2/196 (1%) Frame = +2 Query: 119 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 298 FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT MV++ GEIT+ A V Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTAAKV 63 Query: 299 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RNDEEVGAGDQ 472 DY+K+VR T + IG+ + G D C+V++ ++QQSP+IA GVH + + E++GAGDQ Sbjct: 64 DYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHGHLTKKPEDIGAGDQ 123 Query: 473 GLMFGYATDETEECMPLTVVLAHKLNQKIAELXRNGEFWWARPDSKTQVTCEYVFAGGAT 652 G MFGYATDET E MPLT VLA KL K+ E+ +N W RPD KTQVT EY GGA Sbjct: 124 GHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYKNDGGAM 183 Query: 653 VPQXVHTVXXSLQHSE 700 +P VHTV S QH E Sbjct: 184 IPIRVHTVLISTQHDE 199 >At2g03270.1 68415.m00280 DNA-binding protein, putative similar to Swiss-Prot:Q60560 DNA-binding protein SMUBP-2 (Immunoglobulin MU binding protein 2) (SMUBP-2) (Insulin II gene enhancer-binding protein)(RIPE3B-binding complex 3B2 P110 subunit) (RIP-1)[Mesocricetus auratus]; identical to putative helicase (atpc-2 gene) cDNA NCBI_gi:11191230 Length = 639 Score = 28.3 bits (60), Expect = 5.2 Identities = 13/70 (18%), Positives = 40/70 (57%) Frame = +2 Query: 149 GHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRETV 328 GHP ++ Q+ D+ LDA + + ++ +A + + M L G++ + + ++++++ + Sbjct: 264 GHPARLLPQVLDSALDAQVLKGDNSGLAND--IRKEMKALNGKLLKAKDKNTRRLIQKEL 321 Query: 329 KHIGYDDSSK 358 + +G ++ + Sbjct: 322 RTLGKEERKR 331 >At3g43300.1 68416.m04570 guanine nucleotide exchange family protein similar to SP|Q9Y6D5 Brefeldin A-inhibited guanine nucleotide-exchange protein 2 {Homo sapiens}; contains Pfam profile PF01369: Sec7 domain Length = 1756 Score = 27.9 bits (59), Expect = 6.8 Identities = 18/60 (30%), Positives = 31/60 (51%), Gaps = 3/60 (5%) Frame = +2 Query: 212 DPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRE---TVKHIGYDDSSKGFDYKTCS 382 DP+ +A +T + G L C K+ VD++K+ RE + ++ D +S G +T S Sbjct: 523 DPNPAMASQTASVKGSSLQCLVNVLKSLVDWEKIRREAENSTRNANEDSASTGEPIETKS 582 >At1g54960.1 68414.m06277 NPK1-related protein kinase, putative (ANP2) similar to protein kinase [Nicotiana tabacum] gi|456309|dbj|BAA05648; identical to cDNA NPK1-related protein kinase 2, partial cds GI:2342424 Length = 596 Score = 27.9 bits (59), Expect = 6.8 Identities = 13/49 (26%), Positives = 25/49 (51%) Frame = +2 Query: 203 LNQDPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDD 349 L+ + DAK +C+T+++ +L C + N + + V V H Y + Sbjct: 431 LDNNTDAKKSCDTMSEISDILKCKFDENSGNGETETKVSMEVDHPSYSE 479 >At2g45000.1 68415.m05603 expressed protein contains Pfam profile: PF05064 Nsp1-like C-terminal region Length = 739 Score = 27.5 bits (58), Expect = 9.0 Identities = 21/62 (33%), Positives = 33/62 (53%), Gaps = 2/62 (3%) Frame = -2 Query: 567 SSAIF*LSLCASTTVNGMHSSVSSV--A*PNIKPWSPAPTSSSFLFSCTPAAMFGDCWSS 394 +S++F S A+T+ + + + SS A P+ S AP SSS +F T ++ SS Sbjct: 203 NSSLFGASSSAATSTSPLFGAPSSATGATPSFSVASSAPGSSSSIFGATGSSPSFSVASS 262 Query: 393 AS 388 AS Sbjct: 263 AS 264 >At1g05055.1 68414.m00506 basic transcription factor 2, 44kD subunit-related contains weak similarity to Swiss-Prot:Q13888 TFIIH basal transcription factor complex p44 subunit (Basic transcription factor 2 44 kDa subunit, BTF2-p44, General transcription factor IIH polypeptide 2) [Homo sapiens] Length = 421 Score = 27.5 bits (58), Expect = 9.0 Identities = 13/27 (48%), Positives = 16/27 (59%) Frame = -1 Query: 538 CKHYSQRHAFFCLICCITEHQALVSCP 458 CKHY FCL C I H++L +CP Sbjct: 385 CKHY------FCLDCDIYIHESLHNCP 405 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,783,298 Number of Sequences: 28952 Number of extensions: 378055 Number of successful extensions: 1008 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 971 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1003 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1496852856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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