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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0410
         (700 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati...   256   1e-68
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2...   248   3e-66
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1...   248   4e-66
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1...   248   4e-66
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati...   246   8e-66
At2g03270.1 68415.m00280 DNA-binding protein, putative similar t...    28   5.2  
At3g43300.1 68416.m04570 guanine nucleotide exchange family prot...    28   6.8  
At1g54960.1 68414.m06277 NPK1-related protein kinase, putative (...    28   6.8  
At2g45000.1 68415.m05603 expressed protein contains Pfam profile...    27   9.0  
At1g05055.1 68414.m00506 basic transcription factor 2, 44kD subu...    27   9.0  

>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
           similar to S-adenosylmethionine synthetase 2 (Methionine
           adenosyltransferase 2, AdoMet synthetase 2)
           [Catharanthus roseus] SWISS-PROT:Q96552
          Length = 393

 Score =  256 bits (626), Expect = 1e-68
 Identities = 123/196 (62%), Positives = 147/196 (75%), Gaps = 2/196 (1%)
 Frame = +2

Query: 119 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 298
           FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT MV++ GEIT+KANV
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63

Query: 299 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RNDEEVGAGDQ 472
           DY+++VR+T + IG+  +  G D   C V++ ++QQSP+IA GVH +  +  EEVGAGDQ
Sbjct: 64  DYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKKPEEVGAGDQ 123

Query: 473 GLMFGYATDETEECMPLTVVLAHKLNQKIAELXRNGEFWWARPDSKTQVTCEYVFAGGAT 652
           G MFGYATDET E MPLT VLA KL  K+ E+ +NG   W RPD KTQVT EY+   GA 
Sbjct: 124 GHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGTCPWLRPDGKTQVTIEYINESGAM 183

Query: 653 VPQXVHTVXXSLQHSE 700
           VP  VHTV  S QH E
Sbjct: 184 VPVRVHTVLISTQHDE 199


>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
           identical to S-adenosylmethionine synthetase 2
           (Methionine adenosyltransferase 2, AdoMet synthetase 2)
           [Arabidopsis thaliana] SWISS-PROT:P17562
          Length = 393

 Score =  248 bits (607), Expect = 3e-66
 Identities = 117/196 (59%), Positives = 144/196 (73%), Gaps = 2/196 (1%)
 Frame = +2

Query: 119 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 298
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++ GEIT+KA +
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATI 63

Query: 299 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RNDEEVGAGDQ 472
           DY+K+VR+T + IG+     G D   C V++ ++QQSP+IA GVH +  +  E++GAGDQ
Sbjct: 64  DYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQ 123

Query: 473 GLMFGYATDETEECMPLTVVLAHKLNQKIAELXRNGEFWWARPDSKTQVTCEYVFAGGAT 652
           G MFGYATDET E MPL+ VLA K+  ++ E+ +NG   W RPD KTQVT EY    GA 
Sbjct: 124 GHMFGYATDETPELMPLSHVLATKIGARLTEVRKNGTCRWLRPDGKTQVTVEYYNDNGAM 183

Query: 653 VPQXVHTVXXSLQHSE 700
           VP  VHTV  S QH E
Sbjct: 184 VPVRVHTVLISTQHDE 199


>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
           identical to S-adenosylmethionine synthetase 1
           (Methionine adenosyltransferase 1, AdoMet synthetase 1)
           [Arabidopsis thaliana] SWISS-PROT:P23686
          Length = 393

 Score =  248 bits (606), Expect = 4e-66
 Identities = 120/196 (61%), Positives = 144/196 (73%), Gaps = 2/196 (1%)
 Frame = +2

Query: 119 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 298
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++ GEIT+KA V
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63

Query: 299 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RNDEEVGAGDQ 472
           DY+K+VR+T + IG+     G D   C V++ ++QQSP+IA GVH +  +  EE+GAGDQ
Sbjct: 64  DYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKCPEEIGAGDQ 123

Query: 473 GLMFGYATDETEECMPLTVVLAHKLNQKIAELXRNGEFWWARPDSKTQVTCEYVFAGGAT 652
           G MFGYATDET E MPL+ VLA KL  ++ E+ +NG   W RPD KTQVT EY    GA 
Sbjct: 124 GHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGAM 183

Query: 653 VPQXVHTVXXSLQHSE 700
           VP  VHTV  S QH E
Sbjct: 184 VPIRVHTVLISTQHDE 199


>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
           identical to S-adenosylmethionine synthetase 1
           (Methionine adenosyltransferase 1, AdoMet synthetase 1)
           [Arabidopsis thaliana] SWISS-PROT:P23686
          Length = 393

 Score =  248 bits (606), Expect = 4e-66
 Identities = 120/196 (61%), Positives = 144/196 (73%), Gaps = 2/196 (1%)
 Frame = +2

Query: 119 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 298
           FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVACET TKT MV++ GEIT+KA V
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATV 63

Query: 299 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RNDEEVGAGDQ 472
           DY+K+VR+T + IG+     G D   C V++ ++QQSP+IA GVH +  +  EE+GAGDQ
Sbjct: 64  DYEKIVRDTCRAIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKCPEEIGAGDQ 123

Query: 473 GLMFGYATDETEECMPLTVVLAHKLNQKIAELXRNGEFWWARPDSKTQVTCEYVFAGGAT 652
           G MFGYATDET E MPL+ VLA KL  ++ E+ +NG   W RPD KTQVT EY    GA 
Sbjct: 124 GHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCAWLRPDGKTQVTVEYYNDKGAM 183

Query: 653 VPQXVHTVXXSLQHSE 700
           VP  VHTV  S QH E
Sbjct: 184 VPIRVHTVLISTQHDE 199


>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
           similar to S-adenosylmethionine synthetase 3 (Methionine
           adenosyltransferase 3, AdoMet synthetase 3)
           [Lycopersicon esculentum] SWISS-PROT:P43282
          Length = 390

 Score =  246 bits (603), Expect = 8e-66
 Identities = 119/196 (60%), Positives = 144/196 (73%), Gaps = 2/196 (1%)
 Frame = +2

Query: 119 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANV 298
           FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVACET TKT MV++ GEIT+ A V
Sbjct: 4   FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTAAKV 63

Query: 299 DYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQQSPNIAAGVHEN--RNDEEVGAGDQ 472
           DY+K+VR T + IG+  +  G D   C+V++ ++QQSP+IA GVH +  +  E++GAGDQ
Sbjct: 64  DYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQQSPDIAQGVHGHLTKKPEDIGAGDQ 123

Query: 473 GLMFGYATDETEECMPLTVVLAHKLNQKIAELXRNGEFWWARPDSKTQVTCEYVFAGGAT 652
           G MFGYATDET E MPLT VLA KL  K+ E+ +N    W RPD KTQVT EY   GGA 
Sbjct: 124 GHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNKTCPWLRPDGKTQVTVEYKNDGGAM 183

Query: 653 VPQXVHTVXXSLQHSE 700
           +P  VHTV  S QH E
Sbjct: 184 IPIRVHTVLISTQHDE 199


>At2g03270.1 68415.m00280 DNA-binding protein, putative similar to
           Swiss-Prot:Q60560 DNA-binding protein SMUBP-2
           (Immunoglobulin MU binding protein 2) (SMUBP-2) (Insulin
           II gene enhancer-binding protein)(RIPE3B-binding complex
           3B2 P110 subunit) (RIP-1)[Mesocricetus auratus];
           identical to putative helicase (atpc-2 gene) cDNA
           NCBI_gi:11191230
          Length = 639

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 13/70 (18%), Positives = 40/70 (57%)
 Frame = +2

Query: 149 GHPDKMCDQISDAILDAHLNQDPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRETV 328
           GHP ++  Q+ D+ LDA + +  ++ +A +   +  M  L G++    + + ++++++ +
Sbjct: 264 GHPARLLPQVLDSALDAQVLKGDNSGLAND--IRKEMKALNGKLLKAKDKNTRRLIQKEL 321

Query: 329 KHIGYDDSSK 358
           + +G ++  +
Sbjct: 322 RTLGKEERKR 331


>At3g43300.1 68416.m04570 guanine nucleotide exchange family protein
           similar to SP|Q9Y6D5 Brefeldin A-inhibited guanine
           nucleotide-exchange protein 2 {Homo sapiens}; contains
           Pfam profile PF01369: Sec7 domain
          Length = 1756

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 18/60 (30%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
 Frame = +2

Query: 212 DPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRE---TVKHIGYDDSSKGFDYKTCS 382
           DP+  +A +T +  G  L C     K+ VD++K+ RE   + ++   D +S G   +T S
Sbjct: 523 DPNPAMASQTASVKGSSLQCLVNVLKSLVDWEKIRREAENSTRNANEDSASTGEPIETKS 582


>At1g54960.1 68414.m06277 NPK1-related protein kinase, putative
           (ANP2) similar to protein kinase [Nicotiana tabacum]
           gi|456309|dbj|BAA05648; identical to cDNA NPK1-related
           protein kinase 2, partial cds GI:2342424
          Length = 596

 Score = 27.9 bits (59), Expect = 6.8
 Identities = 13/49 (26%), Positives = 25/49 (51%)
 Frame = +2

Query: 203 LNQDPDAKVACETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDD 349
           L+ + DAK +C+T+++   +L C    +  N + +  V   V H  Y +
Sbjct: 431 LDNNTDAKKSCDTMSEISDILKCKFDENSGNGETETKVSMEVDHPSYSE 479


>At2g45000.1 68415.m05603 expressed protein contains Pfam profile:
           PF05064 Nsp1-like C-terminal region
          Length = 739

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 21/62 (33%), Positives = 33/62 (53%), Gaps = 2/62 (3%)
 Frame = -2

Query: 567 SSAIF*LSLCASTTVNGMHSSVSSV--A*PNIKPWSPAPTSSSFLFSCTPAAMFGDCWSS 394
           +S++F  S  A+T+ + +  + SS   A P+    S AP SSS +F  T ++      SS
Sbjct: 203 NSSLFGASSSAATSTSPLFGAPSSATGATPSFSVASSAPGSSSSIFGATGSSPSFSVASS 262

Query: 393 AS 388
           AS
Sbjct: 263 AS 264


>At1g05055.1 68414.m00506 basic transcription factor 2, 44kD
           subunit-related contains weak similarity to
           Swiss-Prot:Q13888 TFIIH basal transcription factor
           complex p44 subunit (Basic transcription factor 2 44 kDa
           subunit, BTF2-p44, General transcription factor IIH
           polypeptide 2) [Homo sapiens]
          Length = 421

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = -1

Query: 538 CKHYSQRHAFFCLICCITEHQALVSCP 458
           CKHY      FCL C I  H++L +CP
Sbjct: 385 CKHY------FCLDCDIYIHESLHNCP 405


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,783,298
Number of Sequences: 28952
Number of extensions: 378055
Number of successful extensions: 1008
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 971
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1003
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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