Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0388
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC11E10.01 ||SPCC61.06|cystathionine beta-lyase |Schizosacchar... 143 2e-35
SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|c... 99 3e-22
SPAC23A1.14c |||cystathionine gamma-synthase |Schizosaccharomyce... 99 5e-22
SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyce... 52 8e-08
SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomy... 39 6e-04
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 29 0.64
SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces pomb... 28 1.5
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc... 27 2.0
SPBC354.08c |||DUF221 family protein|Schizosaccharomyces pombe|c... 26 4.5
SPAC926.09c |fas1||fatty acid synthase beta subunit Fas1|Schizos... 26 4.5
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 26 4.5
SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr ... 26 4.5
SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr 1||... 26 4.5
SPAC13G6.06c |||glycine cleavage complex subunit P|Schizosacchar... 26 6.0
SPBC25D12.05 |trm1||N2,N2-dimethylguanosine tRNA methyltransfera... 26 6.0
SPAC222.14c |||GTP binding protein Sey1 |Schizosaccharomyces pom... 26 6.0
>SPCC11E10.01 ||SPCC61.06|cystathionine beta-lyase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 390
Score = 143 bits (347), Expect = 2e-35
Identities = 73/190 (38%), Positives = 117/190 (61%), Gaps = 1/190 (0%)
Frame = +3
Query: 39 HIVSSDDVYGGTNRLLRQVVSRLGINTTFTDFTNIEKVKNAMQENTKMIWIETPTNPLLK 218
H+V+ D+YGG++RLL + G D +++ + A++ +T ++ IE+PTNP +
Sbjct: 95 HVVAGHDLYGGSDRLLSFNQRQYGFKVDNVDTSDLAAFEAALRPDTNLVLIESPTNPRIS 154
Query: 219 VVDIEAIVKIARSRG-DIIVVVDNTFLTPYLQKPLDFGADIVMYSLTKYMNGHSDVIMGA 395
+ DI AIVKI RS+ D ++V+DNT L+P L PLDFG DIV S TKY++GH D++ G
Sbjct: 155 ICDIRAIVKITRSKAKDALLVMDNTMLSPVLCNPLDFGYDIVYESATKYLSGHHDLMGGV 214
Query: 396 AIVNDKAIGDKLRFLQNAMGIVPSPIDCYFVNRSLKTLALRMEHHKKSSLIIANWLEKHP 575
I + F NAMG +P +C+ + R +KT+ LR+E +++++ IA +L+
Sbjct: 215 IATKSDEIAKSVFFNINAMGAAMAPFECFLLLRGIKTMGLRVERAQQNAIEIAKFLKS-- 272
Query: 576 KIVEVLHPXL 605
K ++V P L
Sbjct: 273 KGLQVNFPGL 282
>SPBC428.11 |||homocysteine synthase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 429
Score = 99 bits (238), Expect = 3e-22
Identities = 53/135 (39%), Positives = 80/135 (59%), Gaps = 1/135 (0%)
Frame = +3
Query: 12 LVALLNHGDHIVSSDDVYGGTNRLLRQVVSRLGINTTFTDFTNIEKVKNAMQENTKMIWI 191
L L GD+IVS+ +YGGT L + + RLGI T F + + + + ENTK +++
Sbjct: 95 LTTLAKAGDNIVSTSYLYGGTYNLFKVTLPRLGITTKFVNGDDPNDLAAQIDENTKAVYV 154
Query: 192 ETPTNPLLKVVDIEAIVKIARSRGDIIVVVDNTFLTP-YLQKPLDFGADIVMYSLTKYMN 368
E+ NP+ V D E I ++A + G + ++VDNTF YL +P+D GADIV +S TK++
Sbjct: 155 ESIGNPMYNVPDFERIAEVAHAAG-VPLMVDNTFGGGGYLVRPIDHGADIVTHSATKWIG 213
Query: 369 GHSDVIMGAAIVNDK 413
GH I G + + K
Sbjct: 214 GHGTTIGGVIVDSGK 228
Score = 48.8 bits (111), Expect = 7e-07
Identities = 20/50 (40%), Positives = 34/50 (68%)
Frame = +3
Query: 465 SPIDCYFVNRSLKTLALRMEHHKKSSLIIANWLEKHPKIVEVLHPXLXTH 614
+P + + + L+TL+LRME H +++ +A +LEKHPK+ V +P L +H
Sbjct: 279 NPFGVFLLLQGLETLSLRMERHVQNAFALAKYLEKHPKVNWVSYPGLESH 328
>SPAC23A1.14c |||cystathionine gamma-synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 398
Score = 99.1 bits (236), Expect = 5e-22
Identities = 58/169 (34%), Positives = 95/169 (56%)
Frame = +3
Query: 63 YGGTNRLLRQVVSRLGINTTFTDFTNIEKVKNAMQENTKMIWIETPTNPLLKVVDIEAIV 242
Y GT +++ ++ G+ T+F I+ +A+ E +IW+ETP NPL DI
Sbjct: 121 YSGTIQIIARINRLTGLETSF-----IDGKCDAIGEGD-VIWLETPLNPLGIAFDIPFYK 174
Query: 243 KIARSRGDIIVVVDNTFLTPYLQKPLDFGADIVMYSLTKYMNGHSDVIMGAAIVNDKAIG 422
++A+ +G I+VV D+TF P +Q L GAD V++S TKY+ GHSDV+ G D++
Sbjct: 175 ELAKKKGAILVV-DSTFAPPPIQDALVLGADYVVHSATKYLAGHSDVLAGVTASKDRSKI 233
Query: 423 DKLRFLQNAMGIVPSPIDCYFVNRSLKTLALRMEHHKKSSLIIANWLEK 569
L+ + +G + P + + RSL+T LR+ H ++ ++A L K
Sbjct: 234 LDLKADRAYLGTILHPQQAFLLLRSLRTFPLRIAKHSENGFLVAQHLNK 282
>SPBC15D4.09c |||cystathionine gamma-synthase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 610
Score = 52.0 bits (119), Expect = 8e-08
Identities = 45/152 (29%), Positives = 75/152 (49%), Gaps = 4/152 (2%)
Frame = +3
Query: 168 ENTKMIWIETPTNPLLKVVDIEAIVKIARSRGDIIVVVDNTFLTPYLQKPLDFGADIVMY 347
E ++ E P+NPLL D+ I K+A S D VVVD T + L AD+V+
Sbjct: 366 EKVMALFCEFPSNPLLNSPDLVRIRKLADSY-DFAVVVDETIGNFVNVEVLPL-ADVVVS 423
Query: 348 SLTKYMNGHSDVIMGAAIVNDKAIGDKLRFLQNAMGIVPSPI----DCYFVNRSLKTLAL 515
SLTK +G S+V+ G+ ++N + +++AM + + D + R+ + A
Sbjct: 424 SLTKIFSGDSNVMGGSMVLNPSS--RYYSRIKDAMKALYEDLLYDEDALTLERNSRDFAE 481
Query: 516 RMEHHKKSSLIIANWLEKHPKIVEVLHPXLXT 611
R + ++ I N L ++PKI + +P T
Sbjct: 482 RSQVINHNAETICNLLYQNPKIKTLYYPKYNT 513
>SPAC6B12.04c |||aminotransferase class I and II|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 421
Score = 39.1 bits (87), Expect = 6e-04
Identities = 21/54 (38%), Positives = 34/54 (62%), Gaps = 2/54 (3%)
Frame = +3
Query: 138 NIEKVKNAMQENTKMIWIETPTNPLLKVVDIEAIVKIAR--SRGDIIVVVDNTF 293
++ K++NA+ E TKMI I TP NPL K+ E + +IA + +++VV D +
Sbjct: 166 DMNKLRNAITEKTKMIVINTPHNPLGKIFSEEELNEIADLVLKHNLLVVSDEVY 219
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 29.1 bits (62), Expect = 0.64
Identities = 12/45 (26%), Positives = 24/45 (53%)
Frame = +2
Query: 428 TKILAKRHGHRSVTDRLLFCEQELKDVSIKNGASQEVVIDHCQLV 562
T++L K H +++T++ +F QE D +K +DH + +
Sbjct: 74 TELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEELDHYRAI 118
>SPBC27B12.08 |||AP-1 accessory protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1919
Score = 27.9 bits (59), Expect = 1.5
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +3
Query: 117 TTFTDFTNIEKVKNAMQENTKMIWIETPTNPLLKVV 224
T + D N EK + +NT+ ++T NPLL ++
Sbjct: 22 TIYIDLLNYEKQLRNLNKNTREDSLQTNLNPLLSLI 57
>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 767
Score = 27.5 bits (58), Expect = 2.0
Identities = 10/16 (62%), Positives = 12/16 (75%)
Frame = -1
Query: 94 TCLNSLFVPPYTSSED 47
TC N L +PPYTS +D
Sbjct: 734 TCFNRLDLPPYTSKKD 749
>SPBC354.08c |||DUF221 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 865
Score = 26.2 bits (55), Expect = 4.5
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = -1
Query: 367 FIYFVSEYITMSAPKSKGFCKYGVKKVLSTTTIMSPRLLAIFTIASMSTTFN 212
FI V + P F KY + L ++++S LL IFT+ + T FN
Sbjct: 9 FIEVVINSLLNDNPAQAKFDKYSILVSLGFSSLLSVILLCIFTL--LRTKFN 58
>SPAC926.09c |fas1||fatty acid synthase beta subunit
Fas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2073
Score = 26.2 bits (55), Expect = 4.5
Identities = 15/48 (31%), Positives = 26/48 (54%), Gaps = 2/48 (4%)
Frame = +3
Query: 3 TTTLVALLNH--GDHIVSSDDVYGGTNRLLRQVVSRLGINTTFTDFTN 140
+ +++A+LN+ G + VY ++ +V+SR TFTDF N
Sbjct: 1383 SASIIAILNNDSGKTVTVKGTVYRDGKEVI-EVISRFLYRGTFTDFEN 1429
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 26.2 bits (55), Expect = 4.5
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = -1
Query: 94 TCLNSLFVPPYTSSED 47
TC N L +PPY S E+
Sbjct: 638 TCFNRLDIPPYNSKEE 653
>SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 335
Score = 26.2 bits (55), Expect = 4.5
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +2
Query: 80 TIKASSLKIGNQHYIYGLYKY*KSKERHARKHQDD 184
T+ A +K+ N+ Y YGL Y S + R ++DD
Sbjct: 65 TVLAYVMKLLNERYDYGLELYLISVDEGIRGYRDD 99
>SPAC4F10.16c |||P-type ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1367
Score = 26.2 bits (55), Expect = 4.5
Identities = 10/34 (29%), Positives = 17/34 (50%)
Frame = -1
Query: 169 SCMAFFTFSIFVKSVNVVLIPNLETTCLNSLFVP 68
+C F + + V ++ + L+P CL LF P
Sbjct: 1277 TCQTFGFWCVTVLTIALCLLPRFSYICLQKLFYP 1310
>SPAC13G6.06c |||glycine cleavage complex subunit
P|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1017
Score = 25.8 bits (54), Expect = 6.0
Identities = 23/102 (22%), Positives = 44/102 (43%)
Frame = +3
Query: 57 DVYGGTNRLLRQVVSRLGINTTFTDFTNIEKVKNAMQENTKMIWIETPTNPLLKVVDIEA 236
++Y T +LR S GI + T K+A ++ I+++ P + D
Sbjct: 224 NIYPNTLSVLRTRASGFGIKIELDNITPELITKSA--KHVFGIFVQYPAADG-SIFDYGH 280
Query: 237 IVKIARSRGDIIVVVDNTFLTPYLQKPLDFGADIVMYSLTKY 362
+ ARS +V + L+ P ++GAD+ + S ++
Sbjct: 281 LAATARSFNMHVVAATDLLALTILKSPGEWGADVAVGSTQRF 322
>SPBC25D12.05 |trm1||N2,N2-dimethylguanosine tRNA
methyltransferase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 548
Score = 25.8 bits (54), Expect = 6.0
Identities = 12/33 (36%), Positives = 19/33 (57%)
Frame = +2
Query: 479 LFCEQELKDVSIKNGASQEVVIDHCQLVGETSE 577
+F + + K V +KN SQ ++I HC G +E
Sbjct: 293 VFVQIKAKPVLVKNLQSQSLLIYHCSGCGSFAE 325
>SPAC222.14c |||GTP binding protein Sey1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 762
Score = 25.8 bits (54), Expect = 6.0
Identities = 17/53 (32%), Positives = 27/53 (50%), Gaps = 1/53 (1%)
Frame = +3
Query: 111 INTTF-TDFTNIEKVKNAMQENTKMIWIETPTNPLLKVVDIEAIVKIARSRGD 266
+N F T F+ ++ K Q+ TK IW+ N + V+D+E R RG+
Sbjct: 56 LNNLFGTSFSVMDASKR--QQTTKGIWLSKANNSPILVMDVEG--TDGRERGE 104
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,663,065
Number of Sequences: 5004
Number of extensions: 52153
Number of successful extensions: 177
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 166
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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