SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0384
         (750 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC22E12.13c |rpl2403|rpl24-3|60S ribosomal protein L24-3 |Schi...    29   0.54 
SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces po...    27   2.2  
SPCC4G3.05c |mus81||Holliday junction resolvase subunit Mus81|Sc...    27   3.8  
SPAC4H3.11c |ppc89|mug127|spindle pole body protein Ppc89|Schizo...    27   3.8  
SPACUNK4.13c |||GTPase Ylf2 |Schizosaccharomyces pombe|chr 1|||M...    26   5.0  
SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein Msa1|Schizosa...    26   6.6  
SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch...    26   6.6  
SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase Ubp14|Sch...    25   8.7  
SPAC8C9.09c |mug129||sequence orphan|Schizosaccharomyces pombe|c...    25   8.7  
SPBPB21E7.04c ||SPAPB21E7.04c, SPAPB21E7.04c|S-adenosylmethionin...    25   8.7  
SPBC3D6.10 |apn2||AP-endonuclease Apn2|Schizosaccharomyces pombe...    25   8.7  

>SPAC22E12.13c |rpl2403|rpl24-3|60S ribosomal protein L24-3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 192

 Score = 29.5 bits (63), Expect = 0.54
 Identities = 13/35 (37%), Positives = 17/35 (48%)
 Frame = +1

Query: 97  IYRNRGIQFSWFSYKVTMTCRTKCHRRLTMSWSDR 201
           +Y   GI F     KV   CR+KCH+   M  + R
Sbjct: 13  VYPGHGIMFVRNDSKVFRFCRSKCHKNFKMKRNPR 47


>SPAC16.02c |srp2||mRNA export factor Srp2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 365

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 14/27 (51%), Positives = 17/27 (62%), Gaps = 3/27 (11%)
 Frame = -1

Query: 384 RSSRTDCRRPHQ---RRPLREEYHSCR 313
           R+SR+D RRPH    RRP  +EY   R
Sbjct: 244 RNSRSDYRRPHDDEYRRPRGDEYRPGR 270


>SPCC4G3.05c |mus81||Holliday junction resolvase subunit
           Mus81|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 608

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 17/75 (22%), Positives = 29/75 (38%)
 Frame = +1

Query: 115 IQFSWFSYKVTMTCRTKCHRRLTMSWSDRNSQMNQTFWPSI*RVDQPTIHILHKYIKSYR 294
           ++  W +Y +        H     S  + N   ++T       V +PT      Y+ SYR
Sbjct: 64  LEKKWNAYCLENNIPISTHNEQNDSHVNANKSSSETSSEKPRSVKKPTTRKRKVYVPSYR 123

Query: 295 CRHYSSSAGMVLLSK 339
              YS    + +L+K
Sbjct: 124 SGAYSILCALYMLNK 138


>SPAC4H3.11c |ppc89|mug127|spindle pole body protein
           Ppc89|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 783

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 18/65 (27%), Positives = 32/65 (49%)
 Frame = +3

Query: 141 GDNDLSDEMSSTLNDVVVRSKLSNESNFLAVNLKSGSTNYTHFAQIYQIVPVPSLFFIGR 320
           G++++ D M+++  D V R K       L+ +LKS    Y H   +Y+ V +    +I R
Sbjct: 351 GESNVDDGMAASRYDTVKREK-----ERLSEHLKSLQEQYEHIQSVYKNVLLDRESYIMR 405

Query: 321 NGTPL 335
            G  +
Sbjct: 406 LGNKI 410


>SPACUNK4.13c |||GTPase Ylf2 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 407

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 23/102 (22%), Positives = 47/102 (46%), Gaps = 5/102 (4%)
 Frame = +3

Query: 81  IAEAVNLSKQRNTIFVVFIQGDNDLSDEMSST--LNDVVVRSKLSNESNFLAVNLKSGST 254
           I  ++NL   +  +++V +  D+ LSDE  +   + + V ++   ++   L+V  +    
Sbjct: 231 ILNSLNLLTAKPVVYLVNMDQDDYLSDEQEALKGIKEWVEKNSFGDQVIPLSVLFEE--- 287

Query: 255 NYTHFAQIYQIVP---VPSLFFIGRNGTPLEVVCAGVDADNL 371
                 Q++ + P         +G+N    E++CAG  A NL
Sbjct: 288 ------QLFMLTPEEAAQECASLGKNSQLSEIICAGYSALNL 323


>SPAC13G7.13c |msa1|SPAC6C3.01c|RNA-binding protein
           Msa1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 533

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 9/17 (52%), Positives = 12/17 (70%)
 Frame = -1

Query: 363 RRPHQRRPLREEYHSCR 313
           R PHQ++PLR E+   R
Sbjct: 426 RSPHQKKPLRVEFRQLR 442


>SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2176

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 20/76 (26%), Positives = 27/76 (35%), Gaps = 3/76 (3%)
 Frame = +3

Query: 114 NTIFVVFIQGDNDLSDEMSSTLNDVVVRSKLSNESNF---LAVNLKSGSTNYTHFAQIYQ 284
           N + +V I   + L DE    L  +V R     E        V L +   NYT  A    
Sbjct: 646 NLVRLVIIDEVHLLHDERGPVLESIVARIFRHQEETLEQVRLVGLSATLPNYTDVASFLH 705

Query: 285 IVPVPSLFFIGRNGTP 332
           + P   LF+      P
Sbjct: 706 VDPKKGLFYFDSTYRP 721


>SPBC6B1.06c |ubp14|ucp2|ubiquitin C-terminal hydrolase
           Ubp14|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 775

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 12/35 (34%), Positives = 16/35 (45%)
 Frame = +2

Query: 263 TFCTNISNRTGAVIILHRQEWYSSRSGLRWCGRRQ 367
           T+ TN+ N T +   L    W     G   CGR+Q
Sbjct: 164 TYVTNLDNATCSKCDLAENLWMCLTCGALSCGRKQ 198


>SPAC8C9.09c |mug129||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 302

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 17/75 (22%), Positives = 31/75 (41%)
 Frame = +3

Query: 57  TMRWYNGSIAEAVNLSKQRNTIFVVFIQGDNDLSDEMSSTLNDVVVRSKLSNESNFLAVN 236
           ++R +   ++   N+SK R      FI   NDL  ++     D    SK       +   
Sbjct: 57  SLRQFVQQLSVRQNMSKDRREALTYFINSYNDLFHDV--YYGDGRSMSKTEEAQTIVTFT 114

Query: 237 LKSGSTNYTHFAQIY 281
           + S    YTH ++++
Sbjct: 115 IGSYLYKYTHPSELF 129


>SPBPB21E7.04c ||SPAPB21E7.04c,
           SPAPB21E7.04c|S-adenosylmethionine-dependent
           methyltransferase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 281

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 13/38 (34%), Positives = 21/38 (55%)
 Frame = +3

Query: 75  GSIAEAVNLSKQRNTIFVVFIQGDNDLSDEMSSTLNDV 188
           GSI  A N+ +     +V ++QG  D   E  ST+++V
Sbjct: 205 GSIIAADNILRPGVPEYVKYVQGSLDYRKEYDSTVSNV 242


>SPBC3D6.10 |apn2||AP-endonuclease Apn2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 523

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = +3

Query: 396 AEHRQENPKINDHSDAFIKVLDEYSESD 479
           A+ ++E  + N   D  I+VLD  +ESD
Sbjct: 404 AKQKEEKEETNKTEDVSIEVLDNNNESD 431


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,672,325
Number of Sequences: 5004
Number of extensions: 53185
Number of successful extensions: 194
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 194
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -