BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0384
(750 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g43210.2 68415.m05371 UBX domain-containing protein contains ... 58 5e-09
At2g43210.1 68415.m05370 UBX domain-containing protein contains ... 58 5e-09
At2g44860.1 68415.m05585 60S ribosomal protein L24, putative 33 0.15
At4g31210.1 68417.m04432 DNA topoisomerase family protein simila... 31 0.62
At1g53165.1 68414.m06023 protein kinase, putative similar to ser... 31 1.1
At4g29700.1 68417.m04230 type I phosphodiesterase/nucleotide pyr... 30 1.4
At1g16800.1 68414.m02018 tRNA-splicing endonuclease positive eff... 30 1.4
At3g17780.1 68416.m02268 expressed protein 29 2.5
At3g01690.1 68416.m00101 expressed protein 29 2.5
At5g02430.1 68418.m00167 WD-40 repeat family protein contains 6 ... 29 3.3
At1g60180.1 68414.m06779 hypothetical protein similar to hypothe... 28 7.6
>At2g43210.2 68415.m05371 UBX domain-containing protein contains
Pfam profile PF00789: UBX domain
Length = 531
Score = 58.4 bits (135), Expect = 5e-09
Identities = 36/117 (30%), Positives = 68/117 (58%), Gaps = 2/117 (1%)
Frame = +3
Query: 69 YNGSIAEAVNLSKQRNTIFVVFIQGDNDLSDEMSS-TLNDVVVRSKLSNESNFLAVNLKS 245
+ GS+ EA+ +K + +FVV+I G+++ SD+++ T D V LS + V++++
Sbjct: 9 FKGSLPEAIFEAKGKKKLFVVYISGEDEESDKLNRLTWTDASVADSLSKYC--ILVHIQA 66
Query: 246 GSTNYTHFAQIYQIVPVPSLFFIGRNGTPLEVVCAGVDADNLSARIDRI-LAEHRQE 413
GS + T+F+ IY VP + IG +GT + + A++L++ +++ L H QE
Sbjct: 67 GSVDATNFSAIYPYSSVPCIAAIGFSGTQVWRTEGFITAEDLASSLEKAWLGLHIQE 123
>At2g43210.1 68415.m05370 UBX domain-containing protein contains
Pfam profile PF00789: UBX domain
Length = 531
Score = 58.4 bits (135), Expect = 5e-09
Identities = 36/117 (30%), Positives = 68/117 (58%), Gaps = 2/117 (1%)
Frame = +3
Query: 69 YNGSIAEAVNLSKQRNTIFVVFIQGDNDLSDEMSS-TLNDVVVRSKLSNESNFLAVNLKS 245
+ GS+ EA+ +K + +FVV+I G+++ SD+++ T D V LS + V++++
Sbjct: 9 FKGSLPEAIFEAKGKKKLFVVYISGEDEESDKLNRLTWTDASVADSLSKYC--ILVHIQA 66
Query: 246 GSTNYTHFAQIYQIVPVPSLFFIGRNGTPLEVVCAGVDADNLSARIDRI-LAEHRQE 413
GS + T+F+ IY VP + IG +GT + + A++L++ +++ L H QE
Sbjct: 67 GSVDATNFSAIYPYSSVPCIAAIGFSGTQVWRTEGFITAEDLASSLEKAWLGLHIQE 123
>At2g44860.1 68415.m05585 60S ribosomal protein L24, putative
Length = 159
Score = 33.5 bits (73), Expect = 0.15
Identities = 15/43 (34%), Positives = 21/43 (48%)
Frame = +1
Query: 97 IYRNRGIQFSWFSYKVTMTCRTKCHRRLTMSWSDRNSQMNQTF 225
IY GIQF K+ CR+KCH+ M + R + + F
Sbjct: 13 IYPGHGIQFVRNDAKIFRFCRSKCHKNFKMKRNPRKVKWTKAF 55
>At4g31210.1 68417.m04432 DNA topoisomerase family protein similar
to DNA Topoisomerase I (SP:Q9X3X7) {Zymomonas mobilis}
Length = 1280
Score = 31.5 bits (68), Expect = 0.62
Identities = 23/78 (29%), Positives = 35/78 (44%)
Frame = +3
Query: 315 GRNGTPLEVVCAGVDADNLSARIDRILAEHRQENPKINDHSDAFIKVLDEYSESDNYELV 494
G +G E+ A A N + + ++ R +N K+ D S + + VL E V
Sbjct: 333 GSSGIKAELSTAASPASN-GNQATTVKSKRRPKNKKVEDKSSSVVPVL---------EAV 382
Query: 495 CGDEICVRVPRPKHDKPG 548
DE + VP+PKH G
Sbjct: 383 SLDESPISVPKPKHSGSG 400
>At1g53165.1 68414.m06023 protein kinase, putative similar to
serine/threonine protein kinase 24 [Homo sapiens]
SWISS-PROT:Q9Y6E0
Length = 1007
Score = 30.7 bits (66), Expect = 1.1
Identities = 18/42 (42%), Positives = 25/42 (59%), Gaps = 1/42 (2%)
Frame = +3
Query: 147 NDLSDEM-SSTLNDVVVRSKLSNESNFLAVNLKSGSTNYTHF 269
ND +E SS VV+RS S++S+ + + SGST YT F
Sbjct: 729 NDYQEEDDSSGSGTVVIRSPRSSQSSSMFRDQSSGSTRYTSF 770
>At4g29700.1 68417.m04230 type I phosphodiesterase/nucleotide
pyrophosphatase family protein similar to SP|P22413
Ectonucleotide pyrophosphatase/phosphodiesterase 1
(Plasma-cell membrane glycoprotein PC-1) [Includes:
Alkaline phosphodiesterase I (EC 3.1.4.1); Nucleotide
pyrophosphatase (EC 3.6.1.9) {Homo sapiens}; contains
Pfam profile PF01663: Type I phosphodiesterase /
nucleotide pyrophosphatase
Length = 461
Score = 30.3 bits (65), Expect = 1.4
Identities = 14/54 (25%), Positives = 29/54 (53%)
Frame = +3
Query: 135 IQGDNDLSDEMSSTLNDVVVRSKLSNESNFLAVNLKSGSTNYTHFAQIYQIVPV 296
++ ++ + E+ + +N+ + K+ N FL V LK HF++ Y+I P+
Sbjct: 315 VENQSEKNAEVVAKMNEALSSGKVKN-GEFLKVYLKEKLPERLHFSESYRIPPI 367
>At1g16800.1 68414.m02018 tRNA-splicing endonuclease positive
effector-related contains similarity to SEN1, a positive
effector of tRNA-splicing endonuclease [Saccharomyces
cerevisiae] gi|172574|gb|AAB63976
Length = 1939
Score = 30.3 bits (65), Expect = 1.4
Identities = 18/57 (31%), Positives = 35/57 (61%)
Frame = +3
Query: 69 YNGSIAEAVNLSKQRNTIFVVFIQGDNDLSDEMSSTLNDVVVRSKLSNESNFLAVNL 239
Y G I+ +++ + + FV F+Q +ND S+ S + ND+V+ +K E++ + VN+
Sbjct: 941 YYGVIS-VLSIERVDDFHFVRFMQDENDGSNSKSFSENDLVLFTKEHPENSNVGVNM 996
>At3g17780.1 68416.m02268 expressed protein
Length = 129
Score = 29.5 bits (63), Expect = 2.5
Identities = 13/43 (30%), Positives = 25/43 (58%)
Frame = +3
Query: 414 NPKINDHSDAFIKVLDEYSESDNYELVCGDEICVRVPRPKHDK 542
NP + + A ++LD Y +++ + L+C E+C R +H+K
Sbjct: 43 NPAASIVAFAGFQLLDIYWKNE-HRLLCSSEVCTTTERDRHEK 84
>At3g01690.1 68416.m00101 expressed protein
Length = 361
Score = 29.5 bits (63), Expect = 2.5
Identities = 13/26 (50%), Positives = 17/26 (65%)
Frame = +2
Query: 659 LDRNRKQVGTHQATDRSETQRERGEK 736
LDR+R+ V H+ T +S Q ERG K
Sbjct: 325 LDRSRRSVDCHEKTRKSVDQIERGRK 350
>At5g02430.1 68418.m00167 WD-40 repeat family protein contains 6
WD-40 repeats (PF00400); rab11 binding protein, Bos
taurus, EMBL:AF117897
Length = 905
Score = 29.1 bits (62), Expect = 3.3
Identities = 14/27 (51%), Positives = 18/27 (66%), Gaps = 3/27 (11%)
Frame = -3
Query: 364 SASTPAQTTS---RGVPFLPMKNNDGT 293
+ +TP TT+ G+P LP KNNDGT
Sbjct: 781 TTTTPENTTTGKKSGLPPLPKKNNDGT 807
>At1g60180.1 68414.m06779 hypothetical protein similar to
hypothetical protein GI:6017113 from [Arabidopsis
thaliana]
Length = 296
Score = 27.9 bits (59), Expect = 7.6
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +3
Query: 96 NLSKQRNTIFVVFIQGDNDLSDEMSSTLNDVVVRSKL 206
NL +R T+FV + + + D+M LN+ +RSKL
Sbjct: 237 NLGSKRVTLFVELMLKNTKILDKMVVQLNEHYLRSKL 273
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,970,764
Number of Sequences: 28952
Number of extensions: 275715
Number of successful extensions: 863
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 840
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 861
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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