Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0361
(700 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme Rad31|Schiz... 126 4e-30
SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme ... 91 1e-19
SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces ... 65 8e-12
SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme Fub2|Schiz... 58 1e-09
SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces pomb... 55 9e-09
SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces ... 46 4e-06
SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ... 38 0.001
SPBC6B1.05c |||ubiquitin-like conjugating enzyme|Schizosaccharom... 32 0.091
SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyc... 27 2.6
SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit C... 27 3.4
SPAC4A8.14 |prs1||ribose-phosphate pyrophosphokinase Prs1|Schizo... 26 6.0
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 25 7.9
>SPAC4C5.04 |rad31|uba4|SUMO E1-like activator enzyme
Rad31|Schizosaccharomyces pombe|chr 1|||Manual
Length = 307
Score = 126 bits (303), Expect = 4e-30
Identities = 62/172 (36%), Positives = 101/172 (58%)
Frame = +2
Query: 98 VGNNEVELSEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSV 277
+GN+ + E YDRQIRLWG ++Q+ L+ ++VL+I S L EIAKN++L+G+ +
Sbjct: 1 MGNHNINAEEIAL--YDRQIRLWGFNAQQALKQSRVLLITASPLANEIAKNLVLSGIGKL 58
Query: 278 CLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHTKGVDELPDSFF 457
C+LD+ + + D+ QF IG RA ++ LNP+V++ + T + E+ +
Sbjct: 59 CVLDSMTVYEKDVEEQFFIEASDIGQLRANVFKKKLHELNPLVEIDTDTSLISEIDEGKI 118
Query: 458 TEFDVVCATGLKQEQFERINNACRDSNKKFICGDVWGTYGYMFSDLVDHEYS 613
++F +V AT L E+F RIN R N F +G YG+ F DL++H ++
Sbjct: 119 SKFSMVIATQLDYEEFCRINELTRICNASFYATSCFGLYGFAFCDLINHNFA 170
>SPBC1604.21c |ptr3|uba1, SPBC211.09|ubiquitin activating enzyme
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1012
Score = 91.5 bits (217), Expect = 1e-19
Identities = 56/150 (37%), Positives = 79/150 (52%)
Frame = +2
Query: 143 YDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLYS 322
Y RQ+ + G ++ K++ + VLIIG GLG EIAKNV L GVKSV L D + + DL S
Sbjct: 20 YSRQLYVLGHEAMKQMSQSNVLIIGCKGLGVEIAKNVCLAGVKSVTLYDPQPTRIEDLSS 79
Query: 323 QFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHTKGVDELPDSFFTEFDVVCATGLKQEQ 502
Q+ D IGV RA+ ++ + LN V V+ VDEL + F V T +
Sbjct: 80 QYFLTEDDIGVPRAKVTVSKLAELNQYVPVSV----VDELSTEYLKNFKCVVVTETSLTK 135
Query: 503 FERINNACRDSNKKFICGDVWGTYGYMFSD 592
IN+ ++ +I D G +G +F D
Sbjct: 136 QLEINDFTHKNHIAYIAADSRGLFGSIFCD 165
Score = 46.0 bits (104), Expect = 5e-06
Identities = 27/113 (23%), Positives = 58/113 (51%), Gaps = 7/113 (6%)
Frame = +2
Query: 140 QYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKS-----VCLLDNEKLK 304
+YD QI ++G + Q+++ + ++G +G E+ KN + GV + + + D + ++
Sbjct: 409 RYDGQIAVFGSEFQEKIASLSTFLVGAGAIGCEMLKNWAMMGVATGESGHISVTDMDSIE 468
Query: 305 QIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMV--DVTSHTKGVDELPDSFF 457
+ +L QFL P +G ++E + +NP + +TS+ + V + F
Sbjct: 469 KSNLNRQFLFRPRDVGKLKSECASTAVSIMNPSLTGKITSYQERVGPESEGIF 521
>SPAC323.06c |uba5||NEDD8 activating enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 500
Score = 65.3 bits (152), Expect = 8e-12
Identities = 43/167 (25%), Positives = 81/167 (48%), Gaps = 3/167 (1%)
Frame = +2
Query: 122 SEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKL 301
+ A+ ++YDRQ+RLW + Q + + V ++ + +G E KN+IL G+ S ++D+ +
Sbjct: 3 TSAKMQKYDRQVRLWKAEGQNAIEKSHVCLLYANTVGCEALKNLILPGIGSFAVVDDTSV 62
Query: 302 KQIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHTKGVDELPD---SFFTEFDV 472
F D+ G +RA + + LNP V++ + L D +F++F V
Sbjct: 63 DFSMDGMNFFIQYDQEGKSRARCTASLLQQLNPNVEMEYLEMSPEALIDKNIEYFSKFSV 122
Query: 473 VCATGLKQEQFERINNACRDSNKKFICGDVWGTYGYMFSDLVDHEYS 613
V ++ LK++ R+ R + + G G + + HEY+
Sbjct: 123 VLSSNLKEKPLFRLEEYLRSHKIPLLHFNSVGFAGIL--RISTHEYT 167
>SPBC16H5.03c |fub2|uba2|SUMO E1-like activator enzyme
Fub2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 628
Score = 58.0 bits (134), Expect = 1e-09
Identities = 31/121 (25%), Positives = 64/121 (52%), Gaps = 2/121 (1%)
Frame = +2
Query: 170 LDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKI 349
+++ + ++AKVL++G G+G E+ KN++++GVK V ++D + + +L QFL +
Sbjct: 17 VEALRNFKSAKVLLVGAGGIGCELLKNLLMSGVKEVHIIDLDTIDLSNLNRQFLFRKKHV 76
Query: 350 GVNRAEGSLERARGLNPMVDVTSHTKGV--DELPDSFFTEFDVVCATGLKQEQFERINNA 523
+A + + A NP V + ++ + D ++F +FD+V + +N
Sbjct: 77 KQPKAIVAAKTASSFNPNVKLEAYHANIKEDRFNVAWFRQFDLVFNALDNLDARRHVNKQ 136
Query: 524 C 526
C
Sbjct: 137 C 137
>SPAC2G11.10c |||URM1 activating enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 401
Score = 55.2 bits (127), Expect = 9e-09
Identities = 31/108 (28%), Positives = 60/108 (55%), Gaps = 2/108 (1%)
Frame = +2
Query: 104 NNEVELSEAEAEQYDRQIRLW--GLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSV 277
+N +ELS E +Y RQ+ L GL Q L+ + VL+IG GLG + ++ G+ ++
Sbjct: 11 SNGLELSLDEYSRYGRQMLLSEIGLPGQLSLKRSSVLVIGAGGLGCPAMQYLVAAGIGTL 70
Query: 278 CLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSH 421
++D + + + +L+ Q + K G+++A + + LNP V + ++
Sbjct: 71 GIMDGDVVDKSNLHRQIIHSTSKQGMHKAISAKQFLEDLNPNVIINTY 118
>SPAC24H6.12c |uba3||NEDD8 activating enzyme|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 444
Score = 46.4 bits (105), Expect = 4e-06
Identities = 31/117 (26%), Positives = 55/117 (47%)
Frame = +2
Query: 173 DSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKIG 352
++ K ++K+LIIG GLG EI K++ L+G + + ++D + + +L QFL I
Sbjct: 37 ETLKSAFSSKILIIGAGGLGCEILKDLALSGFRDLSVIDMDTIDITNLNRQFLFNESNID 96
Query: 353 VNRAEGSLERARGLNPMVDVTSHTKGVDELPDSFFTEFDVVCATGLKQEQFERINNA 523
+A + P VT + + F+ EF ++ GL + R N+
Sbjct: 97 EPKANVAASMIMKRIPSTVVTPFYGKIQDKTIEFYKEFKLIIC-GLDSVEARRWINS 152
>SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 485
Score = 38.3 bits (85), Expect = 0.001
Identities = 19/92 (20%), Positives = 44/92 (47%)
Frame = +2
Query: 137 EQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDL 316
EQ R +G D +RLR + V+++G G+G+ + + +GV+ + ++D +++ L
Sbjct: 107 EQLARNYAFFGEDGMERLRNSFVIVVGCGGVGSWVINMLARSGVQKIRIVDFDQVSLSSL 166
Query: 317 YSQFLCPPDKIGVNRAEGSLERARGLNPMVDV 412
+ +G + + + P ++V
Sbjct: 167 NRHSIATLQDVGTPKTLAIKKAIKKFAPWIEV 198
>SPBC6B1.05c |||ubiquitin-like conjugating
enzyme|Schizosaccharomyces pombe|chr 2|||Manual
Length = 649
Score = 31.9 bits (69), Expect = 0.091
Identities = 28/113 (24%), Positives = 53/113 (46%), Gaps = 5/113 (4%)
Frame = +2
Query: 119 LSEAEAEQYDRQIRLWGLDSQ---KRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLD 289
LSE+ A + + W L Q R++ +K L++G LG +A+N++ GV+ V +D
Sbjct: 308 LSES-ASTLNLSLMRWRLVPQLDLDRIQNSKCLLLGAGTLGCGVARNLLSWGVRHVTFVD 366
Query: 290 NEKLKQIDLYSQ--FLCPPDKIGVNRAEGSLERARGLNPMVDVTSHTKGVDEL 442
+ + Q F K + +AE + +R + + P + T + + L
Sbjct: 367 YSTVSYSNPVRQSLFTFEDCKRKLPKAECAAQRLKEIYPNMFSTGYNISIPML 419
>SPAC1782.01 ||SPAPYUG7.07|proteasome component|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1679
Score = 27.1 bits (57), Expect = 2.6
Identities = 20/54 (37%), Positives = 25/54 (46%), Gaps = 6/54 (11%)
Frame = -2
Query: 366 SARFTPILSGGHRNWEYRSIC---FSFSLSN---KHTLFTPVRITFFAISAPSP 223
SA IL+GGHRN +S C F L N H FT I +++P P
Sbjct: 806 SAEAMSILAGGHRNVFVKSTCPIFFQKQLDNYKADHYCFTLDFILTDCVNSPKP 859
>SPBC646.11 |cct6||chaperonin-containing T-complex zeta subunit
Cct6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 535
Score = 26.6 bits (56), Expect = 3.4
Identities = 12/19 (63%), Positives = 13/19 (68%)
Frame = +2
Query: 263 GVKSVCLLDNEKLKQIDLY 319
G SVCLL E LKQ +LY
Sbjct: 89 GTTSVCLLVGELLKQAELY 107
>SPAC4A8.14 |prs1||ribose-phosphate pyrophosphokinase
Prs1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 25.8 bits (54), Expect = 6.0
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = +2
Query: 227 LGAEIAKNVILTGVKSVCLLDNEKLKQIDLYSQFLCPPD 343
LG I I+T SVC + ++++LYSQ CP D
Sbjct: 240 LGHIIDDEEIITTPASVC--SEDYAQEVNLYSQGGCPSD 276
>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
Cct1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 25.4 bits (53), Expect = 7.9
Identities = 19/62 (30%), Positives = 27/62 (43%), Gaps = 1/62 (1%)
Frame = +2
Query: 341 DKIGVNRAEGSLERARG-LNPMVDVTSHTKGVDELPDSFFTEFDVVCATGLKQEQFERIN 517
+KI +LER + LN +V TKG+D+L E + K+E RI
Sbjct: 266 EKIREREVMITLERVKKILNAGANVILTTKGIDDLCLKSIIEAGAMAVRRCKKEDLRRIA 325
Query: 518 NA 523
A
Sbjct: 326 KA 327
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,628,889
Number of Sequences: 5004
Number of extensions: 50407
Number of successful extensions: 164
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 159
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 323158234
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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