Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0361
(700 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a) ident... 107 5e-24
At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b) ident... 103 9e-23
At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly... 103 9e-23
At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly... 103 1e-22
At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1... 100 1e-21
At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1... 99 2e-21
At1g05180.1 68414.m00522 auxin-resistance protein AXR1 (AXR1) id... 88 5e-18
At2g32410.1 68415.m03960 auxin-resistance protein, putative stro... 85 6e-17
At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly ... 59 2e-09
At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly ... 59 2e-09
At5g19180.1 68418.m02284 ubiquitin activating enzyme, putative (... 55 4e-08
At5g37530.1 68418.m04520 thiF family protein similar to SP|P3013... 46 3e-05
At1g05350.1 68414.m00542 thiF family protein low similarity to S... 45 6e-05
At5g55130.1 68418.m06872 molybdenum cofactor synthesis protein 3... 44 1e-04
At1g05180.2 68414.m00521 auxin-resistance protein AXR1 (AXR1) id... 34 0.079
At2g06120.1 68415.m00672 hypothetical protein contains Pfam prof... 29 3.0
At4g15900.1 68417.m02416 PP1/PP2A phosphatases pleiotropic regul... 29 3.9
At3g16650.1 68416.m02128 PP1/PP2A phosphatases pleiotropic regul... 29 3.9
At1g63020.1 68414.m07117 DNA-directed RNA polymerase alpha subun... 29 3.9
At5g45300.1 68418.m05561 glycosyl hydrolase family 14 protein si... 27 9.0
At5g05740.2 68418.m00632 peptidase M50 family protein / sterol-r... 27 9.0
At5g05740.1 68418.m00631 peptidase M50 family protein / sterol-r... 27 9.0
>At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a)
identical to SUMO activating enzyme 1a [Arabidopsis
thaliana] GI:22652850; contains Pfam profile PF00899:
ThiF family
Length = 322
Score = 107 bits (258), Expect = 5e-24
Identities = 63/173 (36%), Positives = 93/173 (53%), Gaps = 5/173 (2%)
Frame = +2
Query: 116 ELSEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNE 295
EL+E E YDRQIR+WG ++Q+RL A +L+ G+ G AE KN++L GV SV L+D+
Sbjct: 5 ELTEQETALYDRQIRVWGANAQRRLTKAHILVSGIKGTVAEFCKNIVLAGVGSVTLMDDR 64
Query: 296 KLKQIDLYSQFLCPPDK---IGVNRAEGSLERARGLNPMVDVTSHTKGVDELPDSFFTEF 466
L + FL PPD+ G AE + + NPMV V+ + L FF +F
Sbjct: 65 LANMEALNANFLIPPDENVYSGKTVAEICSDSLKDFNPMVRVSVEKGDLSMLGTDFFEQF 124
Query: 467 DVVCATGLKQEQFERINNACRDSNKK--FICGDVWGTYGYMFSDLVDHEYSEE 619
DVV + + +N CR K+ F D + G +F DL D++Y+++
Sbjct: 125 DVVVIGYGSRATKKYVNEKCRKLKKRVAFYTVDCRDSCGEIFVDLQDYKYTKK 177
>At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b)
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50580;
contains Pfam profile PF00899: ThiF family
Length = 320
Score = 103 bits (248), Expect = 9e-23
Identities = 62/173 (35%), Positives = 93/173 (53%), Gaps = 5/173 (2%)
Frame = +2
Query: 116 ELSEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNE 295
EL+E E YDRQIR+WG +Q+RL + VL+ G+ G AE KN++L GV SV LLD+
Sbjct: 5 ELTEQETALYDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCKNIVLAGVGSVTLLDDR 64
Query: 296 KLKQIDLYSQFLCPPDK---IGVNRAEGSLERARGLNPMVDVTSHTKGVDELPDSFFTEF 466
+ + FL PD+ +G AE + + NPMV V+ + L FF +F
Sbjct: 65 LVTTEVFNANFLILPDENAYVGKTVAEICCDSLKDFNPMVHVSIEKGDLSTLGVDFFEKF 124
Query: 467 DVVCATGLKQEQFERINNACRDSNK--KFICGDVWGTYGYMFSDLVDHEYSEE 619
DVV + + +N CR+ K F D G+ G +F DL +++Y+++
Sbjct: 125 DVVVIGYSSRATKKAVNEKCRNLAKDVAFYTVDCRGSCGEIFVDLQNYKYTKK 177
>At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50680;
contains Pfam profile PF00899: ThiF family
Length = 320
Score = 103 bits (248), Expect = 9e-23
Identities = 62/173 (35%), Positives = 93/173 (53%), Gaps = 5/173 (2%)
Frame = +2
Query: 116 ELSEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNE 295
EL+E E YDRQIR+WG +Q+RL + VL+ G+ G AE KN++L GV SV LLD+
Sbjct: 5 ELTEQETALYDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCKNIVLAGVGSVTLLDDR 64
Query: 296 KLKQIDLYSQFLCPPDK---IGVNRAEGSLERARGLNPMVDVTSHTKGVDELPDSFFTEF 466
+ + FL PD+ +G AE + + NPMV V+ + L FF +F
Sbjct: 65 LVTTEVFNANFLILPDENAYVGKTVAEICCDSLKDFNPMVHVSIEKGDLSTLGVDFFEKF 124
Query: 467 DVVCATGLKQEQFERINNACRDSNK--KFICGDVWGTYGYMFSDLVDHEYSEE 619
DVV + + +N CR+ K F D G+ G +F DL +++Y+++
Sbjct: 125 DVVVIGYSSRATKKAVNEKCRNLAKDVAFYTVDCRGSCGEIFVDLQNYKYTKK 177
>At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly
identical to SUMO activating enzyme 1b [Arabidopsis
thaliana] GI:22652852; nearly identical to At5g50680;
contains Pfam profile PF00899: ThiF family
Length = 318
Score = 103 bits (247), Expect = 1e-22
Identities = 62/172 (36%), Positives = 92/172 (53%), Gaps = 5/172 (2%)
Frame = +2
Query: 116 ELSEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNE 295
EL+E E YDRQIR+WG +Q+RL + VL+ G+ G AE KN++L GV SV LLD+
Sbjct: 5 ELTEQETALYDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCKNIVLAGVGSVTLLDDR 64
Query: 296 KLKQIDLYSQFLCPPDK---IGVNRAEGSLERARGLNPMVDVTSHTKGVDELPDSFFTEF 466
+ + FL PD+ +G AE + + NPMV V+ + L FF +F
Sbjct: 65 LVTTEVFNANFLILPDENAYVGKTVAEICCDSLKDFNPMVHVSIEKGDLSTLGVDFFEKF 124
Query: 467 DVVCATGLKQEQFERINNACRDSNK--KFICGDVWGTYGYMFSDLVDHEYSE 616
DVV + + +N CR+ K F D G+ G +F DL +++Y++
Sbjct: 125 DVVVIGYSSRATKKAVNEKCRNLAKDVAFYTVDCRGSCGEIFVDLQNYKYTK 176
>At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1;
identical to GB:U80808
Length = 1080
Score = 100 bits (239), Expect = 1e-21
Identities = 58/158 (36%), Positives = 92/158 (58%), Gaps = 2/158 (1%)
Frame = +2
Query: 125 EAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLK 304
E + + + RQ+ ++G ++ +RL A+ VLI G+ GLGAEIAKN+IL GVKSV L D ++
Sbjct: 72 EIDEDLHSRQLAVYGRETMRRLFASNVLISGMHGLGAEIAKNLILAGVKSVTLHDERVVE 131
Query: 305 QIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHTKGVDELPDSFFTEFDVVCAT 484
DL S F+ D +G NRA+ S+++ + LN V V+S TK +++ + F VV +
Sbjct: 132 LWDLSSNFVFSEDDVGKNRADASVQKLQDLNNAVVVSSLTKSLNK---EDLSGFQVVVFS 188
Query: 485 GLKQEQFERINNACRDSNK--KFICGDVWGTYGYMFSD 592
+ E+ ++ C F+ DV G +G +F D
Sbjct: 189 DISMERAIEFDDYCHSHQPPIAFVKADVRGLFGSVFCD 226
Score = 50.4 bits (115), Expect = 1e-06
Identities = 32/113 (28%), Positives = 56/113 (49%), Gaps = 5/113 (4%)
Frame = +2
Query: 140 QYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVK-----SVCLLDNEKLK 304
+YD QI ++G QK+L AKV +G LG E KN+ L GV + + D++ ++
Sbjct: 474 RYDAQISVFGAKFQKKLEDAKVFTVGSGALGCEFLKNLALMGVSCGSQGKLTVTDDDIIE 533
Query: 305 QIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHTKGVDELPDSFFTE 463
+ +L QFL IG ++ + A +NP ++ + V ++ F +
Sbjct: 534 KSNLSRQFLFRDWNIGQAKSTVAASAAAVINPRFNIEALQNRVGAETENVFDD 586
>At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1;
identical to gi:1703477
Length = 1077
Score = 99.1 bits (236), Expect = 2e-21
Identities = 60/170 (35%), Positives = 92/170 (54%), Gaps = 2/170 (1%)
Frame = +2
Query: 101 GNNEVELSEAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVC 280
GN+ E + + + RQ+ ++G ++ ++L A+ VLI G+ GLG EIAKN+IL GVKSV
Sbjct: 61 GNDNSNNQEIDEDLHSRQLAVYGRETMRKLFASNVLISGMQGLGVEIAKNIILAGVKSVT 120
Query: 281 LLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHTKGVDELPDSFFT 460
L D ++ DL S F+ + IG NRA S+ + + LN V V++ T +L +
Sbjct: 121 LHDENVVELWDLSSNFVFTEEDIGKNRALASVHKLQELNNAVAVSTLT---GKLTKEQLS 177
Query: 461 EFDVVCATGLKQEQFERINNACRDSNK--KFICGDVWGTYGYMFSDLVDH 604
+F VV + E+ I++ C FI DV G +G +F D H
Sbjct: 178 DFQVVVFVDISFEKATEIDDYCHSHQPPIAFIKADVRGLFGSLFCDFGPH 227
Score = 52.0 bits (119), Expect = 4e-07
Identities = 31/113 (27%), Positives = 58/113 (51%), Gaps = 5/113 (4%)
Frame = +2
Query: 140 QYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVK-----SVCLLDNEKLK 304
+YD QI ++G QK+L A+V ++G LG E KN+ L GV + + D++ ++
Sbjct: 471 RYDAQISVFGSTLQKKLEDARVFVVGAGALGCEFLKNLALMGVSCGTQGKLTVTDDDVIE 530
Query: 305 QIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHTKGVDELPDSFFTE 463
+ +L QFL IG ++ + A G+N +++ + V ++ F +
Sbjct: 531 KSNLSRQFLFRDWNIGQAKSTVAATAAAGINSRLNIDALQNRVGPETENVFDD 583
>At1g05180.1 68414.m00522 auxin-resistance protein AXR1 (AXR1)
identical to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family; identical to cDNA ubiquitin activating
enzyme E1-related protein (AXR1) GI:304103
Length = 540
Score = 88.2 bits (209), Expect = 5e-18
Identities = 52/192 (27%), Positives = 95/192 (49%), Gaps = 6/192 (3%)
Frame = +2
Query: 140 QYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLY 319
+YDRQ+R+WG Q L A + ++ G+E KN++L GV S+ ++D K++ DL
Sbjct: 23 KYDRQLRIWGEVGQAALEEASICLLNCGPTGSEALKNLVLGGVGSITVVDGSKVQFGDLG 82
Query: 320 SQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHTKGVDEL---PDSFFTEFDVVCATGL 490
+ F+ +G ++A+ + LN V+ + D L SFF++F +V AT L
Sbjct: 83 NNFMVDAKSVGQSKAKSVCAFLQELNDSVNAKFIEENPDTLITTNPSFFSQFTLVIATQL 142
Query: 491 KQEQFERINNACRDSNKKFICGDVWGTYGYMFSDLVDHEYSEEIVQHXAXK---RGPDDE 661
++ +++ CRD+N K + +G G++ + +H + H P E
Sbjct: 143 VEDSMLKLDRICRDANVKLVLVRSYGLAGFVRISVKEHPIIDSKPDHFLDDLRLNNPWPE 202
Query: 662 XKNARKTVSITV 697
K+ +T+ + V
Sbjct: 203 LKSFVETIDLNV 214
>At2g32410.1 68415.m03960 auxin-resistance protein, putative strong
similarity to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family
Length = 523
Score = 84.6 bits (200), Expect = 6e-17
Identities = 45/167 (26%), Positives = 84/167 (50%), Gaps = 3/167 (1%)
Frame = +2
Query: 140 QYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLY 319
+YDRQ+R+WG Q L A + ++ G+E KN+++ G+ S+ ++D K++ DL
Sbjct: 7 KYDRQLRIWGELGQSALETASICLLNCGPTGSEALKNLVIGGIGSITIVDGSKVEIGDLG 66
Query: 320 SQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHTKGVDEL---PDSFFTEFDVVCATGL 490
+ F+ +G +RA+ + LN V + D L SFF++F +V AT L
Sbjct: 67 NNFMVDAKSVGQSRAKTVCGFLQELNDSVKANFVEENPDTLISTDPSFFSQFTLVIATQL 126
Query: 491 KQEQFERINNACRDSNKKFICGDVWGTYGYMFSDLVDHEYSEEIVQH 631
++ +++ CR++N + +G G++ + +H E H
Sbjct: 127 VEDSMVKLDRICREANVMLVLARSYGLTGFVRISVKEHTAIETKPDH 173
>At2g21470.2 68415.m02555 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 700
Score = 59.3 bits (137), Expect = 2e-09
Identities = 34/119 (28%), Positives = 62/119 (52%), Gaps = 3/119 (2%)
Frame = +2
Query: 179 QKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKIGVN 358
Q ++ AKVL++G G+G E+ K + L+G + + ++D + ++ +L QFL +G +
Sbjct: 7 QSAIKGAKVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQS 66
Query: 359 RAEGSLERARGLNPMVDVTSHTKGV--DELPDSFFTEFDVVCATGLKQEQFER-INNAC 526
+A+ + + P +++ S+ V E FF +FDVV GL R +N C
Sbjct: 67 KAKVARDAVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVV-LNGLDNLDARRHVNRLC 124
>At2g21470.1 68415.m02554 SUMO activating enzyme 2 (SAE2) nearly
identical to SUMO activating enzyme 2 [Arabidopsis
thaliana] GI:22652854; contains Pfam profiles PF00899:
ThiF family, PF02134: Repeat in ubiquitin-activating
(UBA) protein
Length = 625
Score = 59.3 bits (137), Expect = 2e-09
Identities = 34/119 (28%), Positives = 62/119 (52%), Gaps = 3/119 (2%)
Frame = +2
Query: 179 QKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKIGVN 358
Q ++ AKVL++G G+G E+ K + L+G + + ++D + ++ +L QFL +G +
Sbjct: 7 QSAIKGAKVLMVGAGGIGCELLKTLALSGFEDIHIIDMDTIEVSNLNRQFLFRRSHVGQS 66
Query: 359 RAEGSLERARGLNPMVDVTSHTKGV--DELPDSFFTEFDVVCATGLKQEQFER-INNAC 526
+A+ + + P +++ S+ V E FF +FDVV GL R +N C
Sbjct: 67 KAKVARDAVLRFRPNINIRSYHANVKNPEFDVDFFKQFDVV-LNGLDNLDARRHVNRLC 124
>At5g19180.1 68418.m02284 ubiquitin activating enzyme, putative
(ECR1) identical to putative ubiquitin activating enzyme
E1 [Arabidopsis thaliana] GI:2952433; similar to NEDD8
activating enzyme [Mus musculus] GI:17061821
Length = 454
Score = 55.2 bits (127), Expect = 4e-08
Identities = 26/97 (26%), Positives = 58/97 (59%)
Frame = +2
Query: 200 KVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKIGVNRAEGSLE 379
++L+IG GLG E+ K++ L+G +++ ++D ++++ +L QFL + +G +AE + +
Sbjct: 48 RILVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVTNLNRQFLFRIEDVGKPKAEVAAK 107
Query: 380 RARGLNPMVDVTSHTKGVDELPDSFFTEFDVVCATGL 490
R V++ H +++ F+ +F+++ A GL
Sbjct: 108 RVMERVSGVEIVPHFSRIEDKEIEFYNDFNII-ALGL 143
>At5g37530.1 68418.m04520 thiF family protein similar to SP|P30138
Adenylyltransferase thiF (EC 2.7.7.-) {Escherichia
coli}; contains Pfam profile PF00899: ThiF family
Length = 457
Score = 45.6 bits (103), Expect = 3e-05
Identities = 22/80 (27%), Positives = 45/80 (56%)
Frame = +2
Query: 125 EAEAEQYDRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLK 304
E +E R I+ +GL+SQ ++ + V++IGL G+G+ A ++ +GV + L+D +++
Sbjct: 70 EIVSEHLTRNIQFFGLESQHKVTGSYVVVIGLGGVGSHAASMLLRSGVGKLLLVDFDQVS 129
Query: 305 QIDLYSQFLCPPDKIGVNRA 364
L + +G+ +A
Sbjct: 130 LSSLNRHAVATRADVGIPKA 149
>At1g05350.1 68414.m00542 thiF family protein low similarity to
SP|P30138 Adenylyltransferase thiF (EC 2.7.7.-)
{Escherichia coli}; contains Pfam profile PF00899: ThiF
family
Length = 445
Score = 44.8 bits (101), Expect = 6e-05
Identities = 22/92 (23%), Positives = 51/92 (55%)
Frame = +2
Query: 158 RLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLYSQFLCP 337
R+ +D+ +R+R V I+G+ G+G+ A+ + G+ + L D + ++ ++ F
Sbjct: 70 RMGIVDNYERIREFSVAIVGIGGVGSVAAEMLTRCGIGRLLLYDYDTVELANMNRLFF-R 128
Query: 338 PDKIGVNRAEGSLERARGLNPMVDVTSHTKGV 433
PD++G+ + + +++ +NP V + S T +
Sbjct: 129 PDQVGMTKTDAAVQTLAEINPDVVLESFTMNI 160
>At5g55130.1 68418.m06872 molybdenum cofactor synthesis protein 3 /
molybdopterin synthase sulphurylase (CNX5) identical to
SP|Q9ZNW0 Molybdenum cofactor synthesis protein 3
(Molybdopterin synthase sulfurylase) (MPT synthase
sulfurylase) {Arabidopsis thaliana}; contains Pfam
profiles PF00899: ThiF family, PF00581: Rhodanese-like
domain, PF05237: MoeZ/MoeB domain
Length = 464
Score = 43.6 bits (98), Expect = 1e-04
Identities = 31/158 (19%), Positives = 69/158 (43%), Gaps = 3/158 (1%)
Frame = +2
Query: 119 LSEAEAEQYDRQIRL--WGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVKSVCLLDN 292
LS + +Y RQ+ L + ++ Q L + VL+IG GLG+ + GV + ++D+
Sbjct: 64 LSPDQIYRYSRQLLLPSFAVEGQSNLLKSSVLVIGAGGLGSPALLYLAACGVGQLGIIDH 123
Query: 293 EKLKQIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHTKGV-DELPDSFFTEFD 469
+ ++ +++ Q + IG + + + R +N + V + + + +++D
Sbjct: 124 DVVELNNMHRQIIHTEAFIGHPKVKSAAAACRSINSTIKVDEYVEALRTSNALEILSQYD 183
Query: 470 VVCATGLKQEQFERINNACRDSNKKFICGDVWGTYGYM 583
++ I++ C K + G G G +
Sbjct: 184 IIVDATDNPPSRYMISDCCVLLGKPLVSGAALGMEGQL 221
>At1g05180.2 68414.m00521 auxin-resistance protein AXR1 (AXR1)
identical to SP|P42744 Auxin-resistance protein AXR1
{Arabidopsis thaliana}; contains Pfam profile PF00899:
ThiF family; identical to cDNA ubiquitin activating
enzyme E1-related protein (AXR1) GI:304103
Length = 436
Score = 34.3 bits (75), Expect = 0.079
Identities = 22/91 (24%), Positives = 43/91 (47%), Gaps = 3/91 (3%)
Frame = +2
Query: 434 DELPDSFFTEFDVVCATGLKQEQFERINNACRDSNKKFICGDVWGTYGYMFSDLVDHEYS 613
++ P+ FFT +V AT L ++ +++ CRD+N K + +G G++ + +H
Sbjct: 23 NQKPNLFFT---LVIATQLVEDSMLKLDRICRDANVKLVLVRSYGLAGFVRISVKEHPII 79
Query: 614 EEIVQHXAXK---RGPDDEXKNARKTVSITV 697
+ H P E K+ +T+ + V
Sbjct: 80 DSKPDHFLDDLRLNNPWPELKSFVETIDLNV 110
>At2g06120.1 68415.m00672 hypothetical protein contains Pfam profile
PF03384: Drosophila protein of unknown function, DUF287
Length = 473
Score = 29.1 bits (62), Expect = 3.0
Identities = 14/45 (31%), Positives = 24/45 (53%)
Frame = +2
Query: 488 LKQEQFERINNACRDSNKKFICGDVWGTYGYMFSDLVDHEYSEEI 622
L + F+ +N +D +KK+ +G YG SD+ ++E E I
Sbjct: 286 LMENGFKSMNKRMKDFSKKYEDTGTYGAYGSKESDVRENELDEGI 330
>At4g15900.1 68417.m02416 PP1/PP2A phosphatases pleiotropic
regulator 1 (PRL1) identical to PP1/PP2A phosphatases
pleiotropic regulator PRL1 (SP:Q42384) [Arabidopsis
thaliana], PRL1 [Arabidopsis thaliana] GI:577733;
contains Pfam PF00400: WD domain, G-beta repeat (7
copies)
Length = 486
Score = 28.7 bits (61), Expect = 3.9
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +2
Query: 146 DRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVK-SVC 280
D+Q++ W L+ K +R+ + G+ L +V+LTG + SVC
Sbjct: 239 DKQVKCWDLEQNKVIRSYHGHLSGVYCLALHPTLDVLLTGGRDSVC 284
>At3g16650.1 68416.m02128 PP1/PP2A phosphatases pleiotropic
regulator 2 (PRL2) identical to SP|Q39190 PP1/PP2A
phosphatases pleiotropic regulator PRL2 {Arabidopsis
thaliana}, GB:Q39190 from [Arabidopsis thaliana];
contains Pfam PF00400: WD domain, G-beta repeat (7
copies, 1 weak)
Length = 479
Score = 28.7 bits (61), Expect = 3.9
Identities = 15/46 (32%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = +2
Query: 146 DRQIRLWGLDSQKRLRAAKVLIIGLSGLGAEIAKNVILTGVK-SVC 280
D+Q++ W L+ K +R+ + G+ L +V+LTG + SVC
Sbjct: 233 DKQVKCWDLEQNKVIRSYHGHLHGVYCLALHPTLDVVLTGGRDSVC 278
>At1g63020.1 68414.m07117 DNA-directed RNA polymerase alpha subunit
family protein low similarity to RNA polymerase IIA
largest subunit [Trypanosoma brucei] GI:162215; contains
InterPro accession IPR000722: RNA polymerase, alpha
subunit
Length = 1453
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/44 (29%), Positives = 21/44 (47%)
Frame = +2
Query: 218 LSGLGAEIAKNVILTGVKSVCLLDNEKLKQIDLYSQFLCPPDKI 349
L LG + L V+ C L+ +++Q+ +Y F PP I
Sbjct: 486 LLSLGQDSLTAAYLVNVEKNCYLNRAQMQQLQMYCPFQLPPPAI 529
>At5g45300.1 68418.m05561 glycosyl hydrolase family 14 protein
similar to beta-amylase GI:13560977 from [Castanea
crenata]
Length = 689
Score = 27.5 bits (58), Expect = 9.0
Identities = 17/59 (28%), Positives = 29/59 (49%)
Frame = +2
Query: 293 EKLKQIDLYSQFLCPPDKIGVNRAEGSLERARGLNPMVDVTSHTKGVDELPDSFFTEFD 469
E LK+ + +F+CP ++ N E +L GL+ V + KG+ ++ T FD
Sbjct: 572 ETLKKYSVTVKFVCPGPQMSPNAHEEALADPEGLSWQVINAAWDKGLQIGGENAITCFD 630
>At5g05740.2 68418.m00632 peptidase M50 family protein /
sterol-regulatory element binding protein (SREBP) site 2
protease family protein contains Pfam PF02163:
Sterol-regulatory element binding protein (SREBP) site 2
protease
Length = 527
Score = 27.5 bits (58), Expect = 9.0
Identities = 10/28 (35%), Positives = 18/28 (64%)
Frame = +2
Query: 161 LWGLDSQKRLRAAKVLIIGLSGLGAEIA 244
+WG + RL A + ++GLS L +++A
Sbjct: 439 IWGRKTATRLTGASIALLGLSALFSDVA 466
>At5g05740.1 68418.m00631 peptidase M50 family protein /
sterol-regulatory element binding protein (SREBP) site 2
protease family protein contains Pfam PF02163:
Sterol-regulatory element binding protein (SREBP) site 2
protease
Length = 556
Score = 27.5 bits (58), Expect = 9.0
Identities = 10/28 (35%), Positives = 18/28 (64%)
Frame = +2
Query: 161 LWGLDSQKRLRAAKVLIIGLSGLGAEIA 244
+WG + RL A + ++GLS L +++A
Sbjct: 468 IWGRKTATRLTGASIALLGLSALFSDVA 495
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,634,688
Number of Sequences: 28952
Number of extensions: 264502
Number of successful extensions: 834
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 808
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 826
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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