BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0353 (749 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual 79 5e-16 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 30 0.40 SPBC11B10.06 |sws1||SWIM domain containing-Srs2 interacting prot... 29 0.94 SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po... 27 2.9 SPBC3B9.05 |||helper of TIM |Schizosaccharomyces pombe|chr 2|||M... 27 3.8 SPAC664.15 |||CCR4-Not complex subunit Caf4/Mdv1 |Schizosaccharo... 26 5.0 SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom... 26 5.0 SPAC56F8.16 |esc1||transcription factor Esc1 |Schizosaccharomyce... 26 6.6 SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces po... 25 8.7 SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyce... 25 8.7 SPBC12C2.11 ||SPBC21D10.02|glutamine-fructose-6-phosphate transa... 25 8.7 SPBC685.09 |orc2|orp2|origin recognition complex subunit Orc2|Sc... 25 8.7 >SPBC26H8.04c |||DEP domain|Schizosaccharomyces pombe|chr 2|||Manual Length = 1496 Score = 79.4 bits (187), Expect = 5e-16 Identities = 64/226 (28%), Positives = 105/226 (46%), Gaps = 7/226 (3%) Frame = +3 Query: 12 DWKSLTIPACLPITTDYFPDKRSLQNDYLVSDYNLLPDDVNADFAQNRAIYKDPLTTMEV 191 +W S+ P LP+T YFP + L + + Y + ++ +F Q + E+ Sbjct: 798 NWLSMCTPGALPLTFSYFPSEEELNDKFEEYTYTI---GIDPEFTQ--------MNQQEL 846 Query: 192 FKELVSQRLAQGFQLIVG----VNENEVIESHCASTATPPPSKVAPQCGKPANAAPTKRY 359 E++ QRL++G+Q++V V+ + +++ A T T V Q + Sbjct: 847 LAEMICQRLSRGYQIVVNPAAAVSYSGNPKANKAGTITDFSRSVNNQRNGHLTVG-SDAV 905 Query: 360 LLSIGRI-FHKLTL--VGSTITVTRYRPRHPYPPFNIHYRYRFHAPNHDTYEVSWVSFTT 530 LS+G FH+L+ VG I V RY + + Y Y F + N Y S ++F+ Sbjct: 906 CLSLGDSQFHRLSCDSVGYNIEVKRY-VKKVSGQGKMRYDYNFWSKNECKYVKSNITFSA 964 Query: 531 EKLETYYWNYMDHYICTRGDSDFALVEPLKYWRFRTLLLPLYNPAT 668 Y WNY+D IC G + L + +KYWR R +LLP+ +T Sbjct: 965 NDSANYNWNYVDQLIC--GFETY-LPDSVKYWRARFVLLPMSTTST 1007 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 29.9 bits (64), Expect = 0.40 Identities = 17/56 (30%), Positives = 25/56 (44%) Frame = +3 Query: 282 STATPPPSKVAPQCGKPANAAPTKRYLLSIGRIFHKLTLVGSTITVTRYRPRHPYP 449 ST PPP + PA A+P +G +H T G+ + + +P HP P Sbjct: 905 STLPPPPPTASMTASAPAIASPPPP---KVGETYHPPTASGTRVPPVQ-QPSHPNP 956 >SPBC11B10.06 |sws1||SWIM domain containing-Srs2 interacting protein 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 209 Score = 28.7 bits (61), Expect = 0.94 Identities = 14/50 (28%), Positives = 18/50 (36%) Frame = -1 Query: 458 IEWRIWMXXXXXXXXXXGPDQRKFVEDPPNREQVTLGGCCVGWFPALWGH 309 I+ + W R F E P EQ T GG C+ P + H Sbjct: 145 IDLKYWYCSCSQFSYNAFNSSRSFDEPMPKNEQETWGGRCLSHHPTICSH 194 >SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces pombe|chr 1|||Manual Length = 949 Score = 27.1 bits (57), Expect = 2.9 Identities = 12/34 (35%), Positives = 17/34 (50%) Frame = +3 Query: 405 STITVTRYRPRHPYPPFNIHYRYRFHAPNHDTYE 506 +TI R+R YPP N+ Y + H H+ E Sbjct: 571 TTINNLRFRVLDTYPPNNVIYLFSLHIIFHNELE 604 >SPBC3B9.05 |||helper of TIM |Schizosaccharomyces pombe|chr 2|||Manual Length = 116 Score = 26.6 bits (56), Expect = 3.8 Identities = 10/36 (27%), Positives = 18/36 (50%) Frame = -3 Query: 681 REFVSLPDCREAIVESGTASTSEAQLVRNHCRRAYK 574 R ++ +C I + TSE L+ CR+A++ Sbjct: 18 RRWIDCIECHNEIADHPLLKTSELTLICKKCRKAFR 53 >SPAC664.15 |||CCR4-Not complex subunit Caf4/Mdv1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 651 Score = 26.2 bits (55), Expect = 5.0 Identities = 13/34 (38%), Positives = 17/34 (50%) Frame = -3 Query: 729 HDASXLXICRSGSNRLREFVSLPDCREAIVESGT 628 HD S I S + + F+ DC +A V SGT Sbjct: 480 HDTSTSQILVSDTYKQEPFIRALDCLDAAVASGT 513 >SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 1462 Score = 26.2 bits (55), Expect = 5.0 Identities = 14/52 (26%), Positives = 27/52 (51%) Frame = -3 Query: 624 STSEAQLVRNHCRRAYKCNGPCSSSSKFPVFLS*TKPTTPRTCRGSVRGNDN 469 ST + + + + +K NG SSS ++P+ + ++ T R S+ G D+ Sbjct: 51 STLKDEFLADGLESRWKTNGEKSSSIRYPLVMEFSQLKEDLTNRASLNGYDS 102 >SPAC56F8.16 |esc1||transcription factor Esc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 413 Score = 25.8 bits (54), Expect = 6.6 Identities = 15/48 (31%), Positives = 23/48 (47%) Frame = +1 Query: 313 PHSAGNQPTQHPPSVTCSRLGGSSTNLRWSGPPSPSLDTDPDIHIRHS 456 P SA HP +++ GSS++ +S PP+ S T +HS Sbjct: 94 PTSATLSTAAHPVHTNAAQVAGSSSSYVYSVPPTNS--TTSQASAKHS 139 >SPBP22H7.02c |||RNA-binding protein Mrd1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 833 Score = 25.4 bits (53), Expect = 8.7 Identities = 11/33 (33%), Positives = 19/33 (57%) Frame = -3 Query: 744 LVGPNHDASXLXICRSGSNRLREFVSLPDCREA 646 L P + + I +G+ + EF++ PDCR+A Sbjct: 525 LFSPFGELGRILIPPAGTIAIIEFLNAPDCRQA 557 >SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1210 Score = 25.4 bits (53), Expect = 8.7 Identities = 9/31 (29%), Positives = 15/31 (48%) Frame = +3 Query: 441 PYPPFNIHYRYRFHAPNHDTYEVSWVSFTTE 533 P PF + + HA + Y++SW T+ Sbjct: 1107 PREPFKLVLEFFGHADTNMRYKISWTQLNTQ 1137 >SPBC12C2.11 ||SPBC21D10.02|glutamine-fructose-6-phosphate transaminase |Schizosaccharomyces pombe|chr 2|||Manual Length = 696 Score = 25.4 bits (53), Expect = 8.7 Identities = 15/50 (30%), Positives = 24/50 (48%) Frame = +3 Query: 198 ELVSQRLAQGFQLIVGVNENEVIESHCASTATPPPSKVAPQCGKPANAAP 347 E+V+ R +G LIVGV + ++ P P++ P KP + P Sbjct: 187 EVVATR--RGSPLIVGVKSEQKLKVDFVDVEFPEPAEGLPGTPKPTSLHP 234 >SPBC685.09 |orc2|orp2|origin recognition complex subunit Orc2|Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 25.4 bits (53), Expect = 8.7 Identities = 11/30 (36%), Positives = 16/30 (53%) Frame = +1 Query: 340 QHPPSVTCSRLGGSSTNLRWSGPPSPSLDT 429 Q P ++ +LG R S P SPSL++ Sbjct: 138 QSPQGISVEKLGKEDNRSRSSTPASPSLES 167 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,288,604 Number of Sequences: 5004 Number of extensions: 71817 Number of successful extensions: 209 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 196 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 205 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 357280532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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