Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0345
(750 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 30 1.9
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 30 1.9
At5g49730.1 68418.m06158 ferric reductase-like transmembrane com... 29 3.3
At5g26860.1 68418.m03204 Lon protease homolog 2, mitochondrial a... 29 4.4
At4g09400.1 68417.m01549 hypothetical protein similar to At3g453... 29 4.4
At3g47240.1 68416.m05129 hypothetical protein 29 4.4
At3g49590.2 68416.m05420 expressed protein 28 5.8
At3g49590.1 68416.m05419 expressed protein 28 5.8
At2g35270.1 68415.m04326 DNA-binding protein-related contains Pf... 28 5.8
At1g30690.1 68414.m03752 SEC14 cytosolic factor family protein /... 28 5.8
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 29.9 bits (64), Expect = 1.9
Identities = 20/56 (35%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
Frame = +2
Query: 551 SEEAAGQQAHRDQLRSGRPSSPRGA-LRKQRPRLGAGLSMTPKTWPTAPILNLIKL 715
S + +A L + RP SPR A LR PRL A TP+ P +P+ + +L
Sbjct: 212 SSKPPSPRAEPPTLDTPRPPSPRAASLRADPPRLDAARPTTPR--PPSPLADAPRL 265
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 29.9 bits (64), Expect = 1.9
Identities = 20/56 (35%), Positives = 28/56 (50%), Gaps = 1/56 (1%)
Frame = +2
Query: 551 SEEAAGQQAHRDQLRSGRPSSPRGA-LRKQRPRLGAGLSMTPKTWPTAPILNLIKL 715
S + +A L + RP SPR A LR PRL A TP+ P +P+ + +L
Sbjct: 211 SSKPPSPRAEPPTLDTPRPPSPRAASLRADPPRLDAARPTTPR--PPSPLADAPRL 264
>At5g49730.1 68418.m06158 ferric reductase-like transmembrane
component family protein similar to ferric-chelate
reductase (FRO1) [Pisum sativum] GI:15341529; contains
Pfam profile PF01794: Ferric reductase like
transmembrane componenent
Length = 738
Score = 29.1 bits (62), Expect = 3.3
Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 1/40 (2%)
Frame = -3
Query: 484 MIPKNRGMRAM-IFSLAMAKRGRSCLIFFFLPRASSSDLL 368
++PKNR + + + L + G C++F FLP + S LL
Sbjct: 161 VLPKNRSILLLEVTGLRLGMIGLLCMVFLFLPISRGSILL 200
>At5g26860.1 68418.m03204 Lon protease homolog 2, mitochondrial
almost identical to Lon protease homolog 2
mitochondrial precursor SP:P93655, GI:1848290 from
[Arabidopsis thaliana]
Length = 940
Score = 28.7 bits (61), Expect = 4.4
Identities = 14/39 (35%), Positives = 21/39 (53%)
Frame = +3
Query: 246 LPEEPKAREAQVESRLVDGVADFLENYVVQFKLPSSAVE 362
+PEEP E+ +V V + +EN+ V+ SSA E
Sbjct: 674 VPEEPAVASDPEEAEIVADVGESIENHTVEENTVSSAEE 712
>At4g09400.1 68417.m01549 hypothetical protein similar to At3g45350,
At2g14330
Length = 513
Score = 28.7 bits (61), Expect = 4.4
Identities = 20/62 (32%), Positives = 31/62 (50%)
Frame = -3
Query: 469 RGMRAMIFSLAMAKRGRSCLIFFFLPRASSSDLLMPSTAEDGSLNCTT*FSKKSATPSTS 290
R +R I S+ A+ GR +F+F SS +++ ED + T F +K+ PST
Sbjct: 285 RSIRTAI-SILRARGGRD--LFWFYTGKRSSGIVLTLGCEDRHICSTRTFHRKTTAPSTP 341
Query: 289 LD 284
D
Sbjct: 342 DD 343
>At3g47240.1 68416.m05129 hypothetical protein
Length = 239
Score = 28.7 bits (61), Expect = 4.4
Identities = 21/74 (28%), Positives = 30/74 (40%), Gaps = 2/74 (2%)
Frame = +2
Query: 461 HPPILGYH--RLRSRQSRRTCQDLTPGRRYHCSEEAAGQQAHRDQLRSGRPSSPRGALRK 634
HP ++G RL S + L PG S E Q+ +GR S R +
Sbjct: 85 HPEVVGRKGVRLGEYNSTELMRILPPGPTVRWSVEDGEDQSSDGSDGTGRSSDGRDKCIR 144
Query: 635 QRPRLGAGLSMTPK 676
PR+ G ++T K
Sbjct: 145 THPRVAHGCNLTGK 158
>At3g49590.2 68416.m05420 expressed protein
Length = 603
Score = 28.3 bits (60), Expect = 5.8
Identities = 30/112 (26%), Positives = 42/112 (37%), Gaps = 8/112 (7%)
Frame = +2
Query: 419 PPSLGHRQTKDH----GPHPPILGYHRLRSRQSRRTCQDLTPGRR-YHCSEEAAGQQAHR 583
PP G + H G H P + S L+PG +H S A H+
Sbjct: 276 PPLTGSSAERPHSWTSGFHRPPAQFATPNQSFSPAQSHQLSPGLHDFHWSRTDAFGDNHQ 335
Query: 584 DQ---LRSGRPSSPRGALRKQRPRLGAGLSMTPKTWPTAPILNLIKLLAKNF 730
SG PS+PR PR+ P T P++ LN + ++ NF
Sbjct: 336 LSPPFSPSGSPSTPRYISGGNSPRINVRPGTAPVTIPSSATLN--RYVSSNF 385
>At3g49590.1 68416.m05419 expressed protein
Length = 603
Score = 28.3 bits (60), Expect = 5.8
Identities = 30/112 (26%), Positives = 42/112 (37%), Gaps = 8/112 (7%)
Frame = +2
Query: 419 PPSLGHRQTKDH----GPHPPILGYHRLRSRQSRRTCQDLTPGRR-YHCSEEAAGQQAHR 583
PP G + H G H P + S L+PG +H S A H+
Sbjct: 276 PPLTGSSAERPHSWTSGFHRPPAQFATPNQSFSPAQSHQLSPGLHDFHWSRTDAFGDNHQ 335
Query: 584 DQ---LRSGRPSSPRGALRKQRPRLGAGLSMTPKTWPTAPILNLIKLLAKNF 730
SG PS+PR PR+ P T P++ LN + ++ NF
Sbjct: 336 LSPPFSPSGSPSTPRYISGGNSPRINVRPGTAPVTIPSSATLN--RYVSSNF 385
>At2g35270.1 68415.m04326 DNA-binding protein-related contains Pfam
domain PF03479: Domain of unknown function (DUF296),
found in AT-hook motifs Pfam:PF02178
Length = 285
Score = 28.3 bits (60), Expect = 5.8
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +3
Query: 561 LLASKHTETSYEVVAHPHHEEHYASSGHGWG 653
++A+ T +YE + HEEH S G G G
Sbjct: 212 VMAASFTNVAYERLPLDEHEEHLQSGGGGGG 242
>At1g30690.1 68414.m03752 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to cytosolic factor
(Phosphatidylinositol/phosphatidylcholine transfer
protein) (PI/PCTP) (SP:P24280) [Saccharomyces
cerevisiae]; ESTs gb|T76582, gb|N06574 and gb|Z25700
come from this gene
Length = 540
Score = 28.3 bits (60), Expect = 5.8
Identities = 19/72 (26%), Positives = 34/72 (47%)
Frame = +3
Query: 105 DGDVTLCLKEKALRYVETLRSKREITLVDGVTLDSKGSPRSARALEPLPEEPKAREAQVE 284
+ D LKE + + L+SK E +VD L +K + + ++ EE EA+VE
Sbjct: 57 ESDFFADLKESEKKALSDLKSKLEEAIVDNTLLKTK--KKESSPMKEKKEEVVKPEAEVE 114
Query: 285 SRLVDGVADFLE 320
+ + + +E
Sbjct: 115 KKKEEAAEEKVE 126
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,583,522
Number of Sequences: 28952
Number of extensions: 275972
Number of successful extensions: 879
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 833
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 876
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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