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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0343
         (580 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharo...    93   2e-20
SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizo...    61   1e-10
SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb...    27   2.6  
SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr...    26   3.5  
SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc...    26   4.6  
SPBC16D10.07c |sir2||Sir2 family histone deacetylase Sir2|Schizo...    25   6.1  

>SPBC4C3.07 |||translation initiation factor
           eIF3f|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 302

 Score = 93.5 bits (222), Expect = 2e-20
 Identities = 56/167 (33%), Positives = 89/167 (53%), Gaps = 15/167 (8%)
 Frame = +3

Query: 3   LFQIVDAYERRNADSHRVIGTLLGT--SDKGVVEVTNCFCVPHKEHADQVEAELNYAMDV 176
           LF I+D   R++ ++ RVIGTLLGT   D   +E+ +CF VPH E ++QVE E+ Y   +
Sbjct: 30  LFSILDHSTRKSENNQRVIGTLLGTRSEDGREIEIKSCFAVPHNESSEQVEVEMEYHRAM 89

Query: 177 YELNRRVNSSESIVGWWATGNEVTNHSSVIHEYYSRECREP------------VHVTLDT 320
           Y L+ + N  E +VGW+AT  ++   S++I   Y+    EP            VH+T++T
Sbjct: 90  YHLHLKANPREVVVGWYATSPDLDAFSALIQNLYASPA-EPGTAPLGTYPHPCVHLTVNT 148

Query: 321 SLAGGRMGLRAYVCVPLGVPNG-KQGCMFTPVDVTLTCYEPEIVGLQ 458
            ++   + ++ YV  P+G+       C F P   T+   E    GL+
Sbjct: 149 DVS-SPLAIKTYVSSPVGITERLADSCAFVPTPFTIRDDEAVRSGLK 194


>SPCC1682.10 |rpn8||19S proteasome regulatory subunit
           Rpn8|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 324

 Score = 61.3 bits (142), Expect = 1e-10
 Identities = 39/157 (24%), Positives = 71/157 (45%), Gaps = 5/157 (3%)
 Frame = +3

Query: 3   LFQIVDAYERR-NADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVEA---ELNYAM 170
           L   VD+Y R       RV+G LLG ++  VV V N + +P +E          + N+  
Sbjct: 24  LLSAVDSYNRSAKGTKRRVVGILLGQNNGDVVNVANSYAIPFEEDEKNASVWFLDHNFME 83

Query: 171 DVYELNRRVNSSESIVGWWATGNEVTNHSSVIHEYYSRECREPVHVTLDTSLAGGRMGLR 350
            + E+ +++N++E +VGW+ TG ++      I+    +    PV V +D       +   
Sbjct: 84  SMNEMFKKINANEKLVGWYHTGPQLRPSDLEINNLLKKYIPNPVLVIIDVKPKSVGLPTN 143

Query: 351 AYVCVPLGVPNG-KQGCMFTPVDVTLTCYEPEIVGLQ 458
           AY  +     +G K    F  +  ++   E E +G++
Sbjct: 144 AYFAIDEIEDDGSKSSRTFVHLPSSIEAEEAEEIGVE 180


>SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1334

 Score = 26.6 bits (56), Expect = 2.6
 Identities = 13/42 (30%), Positives = 21/42 (50%)
 Frame = +3

Query: 24  YERRNADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE 149
           Y+ R  DSHR I  L GT  +  +   +   + +K H  ++E
Sbjct: 483 YDHRLRDSHREIYQLQGTGYRPFLRANDNASINNKNHHKELE 524


>SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 131

 Score = 26.2 bits (55), Expect = 3.5
 Identities = 6/11 (54%), Positives = 10/11 (90%)
 Frame = +2

Query: 398 HVHSCRCHSHV 430
           H+H CRCH+++
Sbjct: 69  HIHCCRCHTYI 79


>SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase
           Cdc25|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 596

 Score = 25.8 bits (54), Expect = 4.6
 Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 1/49 (2%)
 Frame = +2

Query: 35  KCRFSQSYRHLIGHERQRSGG-SNQLLLRATQRTCRSSRSGT*LRDGCL 178
           + R S SY   I  ++  SGG + + L  A  RTC  S + T L + CL
Sbjct: 201 RSRSSSSYS--INKKKGTSGGQATRHLTYALSRTCSQSSNTTSLLESCL 247


>SPBC16D10.07c |sir2||Sir2 family histone deacetylase
           Sir2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 475

 Score = 25.4 bits (53), Expect = 6.1
 Identities = 13/27 (48%), Positives = 17/27 (62%)
 Frame = +1

Query: 205 LKVLLVGGRLAMK*PTTPLLYTSITPV 285
           LKV  V   +++  PTTP +Y S TPV
Sbjct: 376 LKVAPVSELISVIPPTTPQIYISRTPV 402


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,263,104
Number of Sequences: 5004
Number of extensions: 47328
Number of successful extensions: 131
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 126
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 130
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 248115846
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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