BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= brP-0343 (580 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharo... 93 2e-20 SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizo... 61 1e-10 SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pomb... 27 2.6 SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr... 26 3.5 SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc... 26 4.6 SPBC16D10.07c |sir2||Sir2 family histone deacetylase Sir2|Schizo... 25 6.1 >SPBC4C3.07 |||translation initiation factor eIF3f|Schizosaccharomyces pombe|chr 2|||Manual Length = 302 Score = 93.5 bits (222), Expect = 2e-20 Identities = 56/167 (33%), Positives = 89/167 (53%), Gaps = 15/167 (8%) Frame = +3 Query: 3 LFQIVDAYERRNADSHRVIGTLLGT--SDKGVVEVTNCFCVPHKEHADQVEAELNYAMDV 176 LF I+D R++ ++ RVIGTLLGT D +E+ +CF VPH E ++QVE E+ Y + Sbjct: 30 LFSILDHSTRKSENNQRVIGTLLGTRSEDGREIEIKSCFAVPHNESSEQVEVEMEYHRAM 89 Query: 177 YELNRRVNSSESIVGWWATGNEVTNHSSVIHEYYSRECREP------------VHVTLDT 320 Y L+ + N E +VGW+AT ++ S++I Y+ EP VH+T++T Sbjct: 90 YHLHLKANPREVVVGWYATSPDLDAFSALIQNLYASPA-EPGTAPLGTYPHPCVHLTVNT 148 Query: 321 SLAGGRMGLRAYVCVPLGVPNG-KQGCMFTPVDVTLTCYEPEIVGLQ 458 ++ + ++ YV P+G+ C F P T+ E GL+ Sbjct: 149 DVS-SPLAIKTYVSSPVGITERLADSCAFVPTPFTIRDDEAVRSGLK 194 >SPCC1682.10 |rpn8||19S proteasome regulatory subunit Rpn8|Schizosaccharomyces pombe|chr 3|||Manual Length = 324 Score = 61.3 bits (142), Expect = 1e-10 Identities = 39/157 (24%), Positives = 71/157 (45%), Gaps = 5/157 (3%) Frame = +3 Query: 3 LFQIVDAYERR-NADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVEA---ELNYAM 170 L VD+Y R RV+G LLG ++ VV V N + +P +E + N+ Sbjct: 24 LLSAVDSYNRSAKGTKRRVVGILLGQNNGDVVNVANSYAIPFEEDEKNASVWFLDHNFME 83 Query: 171 DVYELNRRVNSSESIVGWWATGNEVTNHSSVIHEYYSRECREPVHVTLDTSLAGGRMGLR 350 + E+ +++N++E +VGW+ TG ++ I+ + PV V +D + Sbjct: 84 SMNEMFKKINANEKLVGWYHTGPQLRPSDLEINNLLKKYIPNPVLVIIDVKPKSVGLPTN 143 Query: 351 AYVCVPLGVPNG-KQGCMFTPVDVTLTCYEPEIVGLQ 458 AY + +G K F + ++ E E +G++ Sbjct: 144 AYFAIDEIEDDGSKSSRTFVHLPSSIEAEEAEEIGVE 180 >SPCC645.07 |rgf1||RhoGEF for Rho1, Rgf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1334 Score = 26.6 bits (56), Expect = 2.6 Identities = 13/42 (30%), Positives = 21/42 (50%) Frame = +3 Query: 24 YERRNADSHRVIGTLLGTSDKGVVEVTNCFCVPHKEHADQVE 149 Y+ R DSHR I L GT + + + + +K H ++E Sbjct: 483 YDHRLRDSHREIYQLQGTGYRPFLRANDNASINNKNHHKELE 524 >SPAPJ691.02 |||yippee-like protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 131 Score = 26.2 bits (55), Expect = 3.5 Identities = 6/11 (54%), Positives = 10/11 (90%) Frame = +2 Query: 398 HVHSCRCHSHV 430 H+H CRCH+++ Sbjct: 69 HIHCCRCHTYI 79 >SPAC24H6.05 |cdc25|sal2|serine/threonine protein phosphatase Cdc25|Schizosaccharomyces pombe|chr 1|||Manual Length = 596 Score = 25.8 bits (54), Expect = 4.6 Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 1/49 (2%) Frame = +2 Query: 35 KCRFSQSYRHLIGHERQRSGG-SNQLLLRATQRTCRSSRSGT*LRDGCL 178 + R S SY I ++ SGG + + L A RTC S + T L + CL Sbjct: 201 RSRSSSSYS--INKKKGTSGGQATRHLTYALSRTCSQSSNTTSLLESCL 247 >SPBC16D10.07c |sir2||Sir2 family histone deacetylase Sir2|Schizosaccharomyces pombe|chr 2|||Manual Length = 475 Score = 25.4 bits (53), Expect = 6.1 Identities = 13/27 (48%), Positives = 17/27 (62%) Frame = +1 Query: 205 LKVLLVGGRLAMK*PTTPLLYTSITPV 285 LKV V +++ PTTP +Y S TPV Sbjct: 376 LKVAPVSELISVIPPTTPQIYISRTPV 402 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,263,104 Number of Sequences: 5004 Number of extensions: 47328 Number of successful extensions: 131 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 126 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 130 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 248115846 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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