Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= brP-0328
(750 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 231 1e-61
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 113 2e-26
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 109 3e-25
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 95 7e-21
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 94 2e-20
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 93 4e-20
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 84 2e-17
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 81 1e-16
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 79 9e-16
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 77 3e-15
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 66 5e-12
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 59 8e-10
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 56 7e-09
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 38 0.002
SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces ... 30 0.31
SPBC4B4.03 |rsc1||RSC complex subunit Rsc1 |Schizosaccharomyces ... 28 1.6
SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|... 28 1.6
SPBC16G5.15c |fkh2||fork head transcription factor Fkh2 |Schizos... 27 3.8
SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces po... 25 8.7
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 231 bits (564), Expect = 1e-61
Identities = 96/146 (65%), Positives = 120/146 (82%)
Frame = +3
Query: 138 MSGIASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKL 317
MS + RL EERK WR+DHPFGF A+P K+ DG L+LM W+ IPGK T WEGGLYKL
Sbjct: 1 MSSLCKTRLQEERKQWRRDHPFGFYAKPCKSSDGGLDLMNWKVGIPGKPKTSWEGGLYKL 60
Query: 318 RMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDLL 497
M F ++YP+ PPKC+F PPLFHPNVYPSGTVCLS+L+EE+ W+PAITIKQILLGIQDLL
Sbjct: 61 TMAFPEEYPTRPPKCRFTPPLFHPNVYPSGTVCLSILNEEEGWKPAITIKQILLGIQDLL 120
Query: 498 NEPNVKDPAQAEAYTIYCQNRLEYDK 575
++PN+ PAQ EAYT++ ++++EY+K
Sbjct: 121 DDPNIASPAQTEAYTMFKKDKVEYEK 146
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 113 bits (273), Expect = 2e-26
Identities = 58/144 (40%), Positives = 80/144 (55%)
Frame = +3
Query: 138 MSGIASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKL 317
MS A RL + K ++D P G A P+ + N+M W I G TP+E G +KL
Sbjct: 1 MSTTARRRLMRDFKRMQQDPPAGVSASPVSD-----NVMLWNAVIIGPADTPFEDGTFKL 55
Query: 318 RMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDLL 497
+ F + YP+ PP KF +FHPNVY +G +CL +L + W P + IL IQ LL
Sbjct: 56 VLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCLDIL--QNRWSPTYDVAAILTSIQSLL 113
Query: 498 NEPNVKDPAQAEAYTIYCQNRLEY 569
N+PN PA AEA ++ +N+ EY
Sbjct: 114 NDPNNASPANAEAAQLHRENKKEY 137
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 109 bits (263), Expect = 3e-25
Identities = 59/152 (38%), Positives = 80/152 (52%), Gaps = 11/152 (7%)
Frame = +3
Query: 147 IASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLRMI 326
+A RL +E K ++ P G A P D TW+C I G GTP+EGGLY +
Sbjct: 5 MALRRLMKEYKELTENGPDGITAGPSNEDD----FFTWDCLIQGPDGTPFEGGLYPATLK 60
Query: 327 FKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKD-----------WRPAITIKQI 473
F DYP PP KFE FHPNVY GTVC+S+L D W P ++++I
Sbjct: 61 FPSDYPLGPPTLKFECEFFHPNVYKDGTVCISILHAPGDDPNMYESSSERWSPVQSVEKI 120
Query: 474 LLGIQDLLNEPNVKDPAQAEAYTIYCQNRLEY 569
LL + +L EPN + A +A ++ ++R EY
Sbjct: 121 LLSVMSMLAEPNDESGANIDACKMWREDREEY 152
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 95.5 bits (227), Expect = 7e-21
Identities = 49/142 (34%), Positives = 76/142 (53%)
Frame = +3
Query: 147 IASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLRMI 326
+A R+ E KD P A P+ + +L W+ I G +P+ GG++ L +
Sbjct: 1 MALKRINRELADLGKDPPSSCSAGPVGD-----DLFHWQATIMGPADSPYAGGVFFLSIH 55
Query: 327 FKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDLLNEP 506
F DYP PPK F ++HPN+ +G++CL +L ++ W PA+TI ++LL I LL +P
Sbjct: 56 FPTDYPFKPPKVNFTTRIYHPNINSNGSICLDILRDQ--WSPALTISKVLLSICSLLTDP 113
Query: 507 NVKDPAQAEAYTIYCQNRLEYD 572
N DP E +Y +R Y+
Sbjct: 114 NPDDPLVPEIAHVYKTDRSRYE 135
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 93.9 bits (223), Expect = 2e-20
Identities = 44/115 (38%), Positives = 65/115 (56%)
Frame = +3
Query: 231 PDGSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGT 410
PD NL+ W I G T +EG +K+ M F +YP SPP F P++HPNV SG
Sbjct: 52 PDSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTSPMWHPNVDMSGN 111
Query: 411 VCLSLLDEEKDWRPAITIKQILLGIQDLLNEPNVKDPAQAEAYTIYCQNRLEYDK 575
+CL +L ++ W ++ ILL +Q LL EPN P A+A ++ ++ +EY +
Sbjct: 112 ICLDILKDK--WSAVYNVQTILLSLQSLLGEPNNASPLNAQAAELWSKDPIEYKR 164
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 93.1 bits (221), Expect = 4e-20
Identities = 47/133 (35%), Positives = 74/133 (55%)
Frame = +3
Query: 159 RLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDD 338
R+ +E + +D P G VA P ++ NL ++ + G + + +EGG + L + D+
Sbjct: 6 RIIKEIETLTRDPPPGIVAAPTED-----NLRYFKITMEGPQQSAYEGGKFHLELFLPDE 60
Query: 339 YPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQILLGIQDLLNEPNVKD 518
YP PP +F ++HPNV G +CLS L +KDW PA+ I+ +LL IQ L+ PN D
Sbjct: 61 YPMMPPNVRFLTKIYHPNVDKLGRICLSTL--KKDWSPALQIRTVLLSIQALMGAPNPDD 118
Query: 519 PAQAEAYTIYCQN 557
P + I+ +N
Sbjct: 119 PLDNDVAKIWKEN 131
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 83.8 bits (198), Expect = 2e-17
Identities = 48/156 (30%), Positives = 79/156 (50%), Gaps = 11/156 (7%)
Frame = +3
Query: 141 SGIASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLR 320
S + L ++ K +K+ P GF + + ++ WE I G + T +EGG +
Sbjct: 3 SSASEQLLRKQLKEIQKNPPQGFSVGLVDDK----SIFEWEVMIIGPEDTLYEGGFFHAT 58
Query: 321 MIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLL-----------DEEKDWRPAITIK 467
+ F DYP PPK KF ++HPNV+P+G VC+S+L D + W P + +
Sbjct: 59 LSFPQDYPLMPPKMKFTTEIWHPNVHPNGEVCISILHPPGDDKYGYEDAGERWLPVHSPE 118
Query: 468 QILLGIQDLLNEPNVKDPAQAEAYTIYCQNRLEYDK 575
IL+ + +L+ PN + PA +A + +N E+ K
Sbjct: 119 TILISVISMLSSPNDESPANIDAAKEFRENPQEFKK 154
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 81.4 bits (192), Expect = 1e-16
Identities = 46/145 (31%), Positives = 73/145 (50%), Gaps = 1/145 (0%)
Frame = +3
Query: 138 MSGIASARLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKL 317
MS S R+A+E ++D G + + L M G +GTP+EGG + +
Sbjct: 1 MSDNRSRRIAKELADVQQDKQAGIQVWTINDDISHLKGM-----FRGPEGTPYEGGYFVV 55
Query: 318 RMIFKDDYPSSPPKCKFEPPLFHPNV-YPSGTVCLSLLDEEKDWRPAITIKQILLGIQDL 494
+ DYP PPK F+ ++HPNV +G +CL +L ++ W P T+K L+ +Q L
Sbjct: 56 DIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAICLDILKDQ--WSPVYTMKSALISLQSL 113
Query: 495 LNEPNVKDPAQAEAYTIYCQNRLEY 569
L P +P A+ +Y QN ++
Sbjct: 114 LCTPEPSNPQDAQVAQVYLQNYQQF 138
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 78.6 bits (185), Expect = 9e-16
Identities = 38/110 (34%), Positives = 58/110 (52%)
Frame = +3
Query: 246 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 425
NL W C G + + GG + + F DYP PP +F ++HPN G VCL++
Sbjct: 35 NLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIYHPNFDSEGNVCLAI 94
Query: 426 LDEEKDWRPAITIKQILLGIQDLLNEPNVKDPAQAEAYTIYCQNRLEYDK 575
L +++ ++P+I ++ +L I LL EPN DP A Y +R +DK
Sbjct: 95 L-KQQVFKPSIKLRSVLEQILQLLREPNPDDPLVASIAEQYRNDRPSFDK 143
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 77.0 bits (181), Expect = 3e-15
Identities = 33/81 (40%), Positives = 49/81 (60%)
Frame = +3
Query: 294 WEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSLLDEEKDWRPAITIKQI 473
++GG +K R+ D+YP PPK K ++HPN+ G VCL++L +DW P + + I
Sbjct: 65 YKGGKFKFRIQIDDNYPHDPPKVKCLNKIYHPNIDIEGNVCLNIL--RQDWNPVLNLNSI 122
Query: 474 LLGIQDLLNEPNVKDPAQAEA 536
L+G+Q L PN +DP EA
Sbjct: 123 LVGLQFLFLSPNAEDPLNKEA 143
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 66.1 bits (154), Expect = 5e-12
Identities = 32/103 (31%), Positives = 54/103 (52%), Gaps = 1/103 (0%)
Frame = +3
Query: 231 PDGSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNV-YPSG 407
P +++ W+ I G TP+EGG + L + + YP SPP F+ + HPN+ + +G
Sbjct: 28 PVTDVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPNISWTNG 87
Query: 408 TVCLSLLDEEKDWRPAITIKQILLGIQDLLNEPNVKDPAQAEA 536
VC+ +L + W PA +++ L I LL+ + P +A
Sbjct: 88 EVCMDIL--KTHWSPAWSLQSACLAIISLLSNYDASSPLNVDA 128
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 58.8 bits (136), Expect = 8e-10
Identities = 31/99 (31%), Positives = 49/99 (49%), Gaps = 2/99 (2%)
Frame = +3
Query: 246 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYP-SGTVCLS 422
N+ + G TP+ GG++K+ + +YP P F +FHPN+ SG+VCL
Sbjct: 28 NMQEFYVRFHGPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSVCLD 87
Query: 423 LLDEEKDWRPAITIKQIL-LGIQDLLNEPNVKDPAQAEA 536
++++ W P + I + + LL PN DP EA
Sbjct: 88 VINQ--TWSPMFDMINIFEVFLPQLLRYPNASDPLNGEA 124
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 55.6 bits (128), Expect = 7e-09
Identities = 28/90 (31%), Positives = 41/90 (45%), Gaps = 1/90 (1%)
Frame = +3
Query: 246 NLMTWECAIPGKKGTPWEGGLYKLRMIFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSL 425
N++ W I G TP+EGG Y +IF DYP PP + P + +CLS
Sbjct: 33 NILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMITP--SGRFQTNTRLCLSF 90
Query: 426 LD-EEKDWRPAITIKQILLGIQDLLNEPNV 512
D K W P+ + IL+G+ + +
Sbjct: 91 SDFHPKSWNPSWMVSTILVGLVSFMTSDEI 120
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 37.9 bits (84), Expect = 0.002
Identities = 31/132 (23%), Positives = 57/132 (43%), Gaps = 2/132 (1%)
Frame = +3
Query: 159 RLAEERKAWRKDHPFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYKLRMIFKDD 338
+L EE + K + + N D + L W I G + E +Y L++ +
Sbjct: 9 KLLEELEKGEKGLGESSCSYGLTNAD-DITLSDWNATILGPAHSVHENRIYSLKIHCDAN 67
Query: 339 YPSSPPKCKFEPPLFHPNV-YPSGTVCLSLLDEEKDWRPAITIKQILLGI-QDLLNEPNV 512
YP +PP F + P V +G V +D + W+ +++ +LL + +++ + N
Sbjct: 68 YPDAPPIVTFVSRINLPGVDGETGKVNPHKIDCLRHWKREYSMETVLLDLKKEMASSSNR 127
Query: 513 KDPAQAEAYTIY 548
K P E T +
Sbjct: 128 KLPQPPEGSTFF 139
>SPAC12G12.14c |pfs2||WD repeat protein Pfs2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 509
Score = 30.3 bits (65), Expect = 0.31
Identities = 20/58 (34%), Positives = 28/58 (48%), Gaps = 4/58 (6%)
Frame = -3
Query: 367 NLHFGGLDG*SSLKIIRSLYSPPSHGVPFLPGIAHSQVI-KFNEPS---GFFIGLATK 206
N F G+ L + S SP HG P +PG+ H+ + + EPS F GL +K
Sbjct: 442 NPQFSGVLNLPGLGTMPSFPSPYQHGQPQIPGMLHASLSNSYAEPSTQNSFIPGLTSK 499
>SPBC4B4.03 |rsc1||RSC complex subunit Rsc1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 803
Score = 27.9 bits (59), Expect = 1.6
Identities = 17/50 (34%), Positives = 23/50 (46%)
Frame = -1
Query: 309 TAHLPMESPFYQVSHIPKSLNSMNHLDSS*ASRQNQRDGPYAKLSVLQLN 160
T+ +P PFY S +SLN+ H + + PYAKLS N
Sbjct: 613 TSPIPAR-PFYAQSGSLQSLNTTQHSHQISGGHSGRMNVPYAKLSYTSHN 661
>SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 574
Score = 27.9 bits (59), Expect = 1.6
Identities = 12/36 (33%), Positives = 22/36 (61%)
Frame = -3
Query: 196 WSLRQAFRSSAKRALAIPDIFYKNNSIYEPSFNQGL 89
W LR+ +S++ + ++F K NSIY +F++ L
Sbjct: 71 WQLREQEQSNSHISRNENELFSKENSIYNGNFSENL 106
>SPBC16G5.15c |fkh2||fork head transcription factor Fkh2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 642
Score = 26.6 bits (56), Expect = 3.8
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +3
Query: 198 PFGFVARPMKNPDGSLNLMTWECAIPGKKGTPWEGGLYK 314
P V+R + L+ TW C++ G+ G +G L+K
Sbjct: 123 PSKVVSRKHAVVEYDLDDQTWNCSVYGRNGIKVDGKLFK 161
>SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 472
Score = 25.4 bits (53), Expect = 8.7
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = -1
Query: 138 FSTKITRFMSRASIKDYS*CWAID 67
FS +ITRF R D+ CW D
Sbjct: 343 FSAEITRFADRNFYDDWWNCWTWD 366
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,018,484
Number of Sequences: 5004
Number of extensions: 65544
Number of successful extensions: 175
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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