Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= brP-0327
         (683 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBP8B7.24c |atg8||autophagy associated protein Atg8 |Schizosacc...   136   4e-33
SPBC18H10.04c |sce3|tif48|translation initiation factor eIF4B|Sc...    27   3.3  
SPBC21C3.17c |||conserved fungal protein|Schizosaccharomyces pom...    26   5.8  

>SPBP8B7.24c |atg8||autophagy associated protein Atg8
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 121

 Score =  136 bits (328), Expect = 4e-33
 Identities = 59/117 (50%), Positives = 82/117 (70%)
 Frame = +3

Query: 66  SNTKKNIHLRRERLRAKKSAGKYPDRVPVIVEKAPKARLGDLDKKKYLVPSDLTVGQFYF 245
           S  K +    + +  +++   KYPDR+PVI EK  K+ +  +DKKKYLVPSDLTVGQF +
Sbjct: 3   SQFKDDFSFEKRKTESQRIREKYPDRIPVICEKVDKSDIAAIDKKKYLVPSDLTVGQFVY 62

Query: 246 LIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHDEDFFLYIAFSDENVYGN*F 416
           +IRKRI L PE A+F F++ ++PPT+A M ++Y+EH  ED FLYI +S EN +G  F
Sbjct: 63  VIRKRIKLSPEKAIFIFIDEILPPTAALMSTIYEEHKSEDGFLYITYSGENTFGTVF 119



 Score = 28.3 bits (60), Expect = 1.1
 Identities = 10/20 (50%), Positives = 16/20 (80%)
 Frame = +2

Query: 59  MKFQYKEEHSFEKRKAEGEK 118
           M+ Q+K++ SFEKRK E ++
Sbjct: 1   MRSQFKDDFSFEKRKTESQR 20


>SPBC18H10.04c |sce3|tif48|translation initiation factor
           eIF4B|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 388

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 19/62 (30%), Positives = 31/62 (50%), Gaps = 3/62 (4%)
 Frame = +3

Query: 48  KIST*NSNTKKNIHLRRERLRAKK-SAGKYP--DRVPVIVEKAPKARLGDLDKKKYLVPS 218
           K++    + ++  +  RER  ++K SA K    D+   I EK    RLGD +KK     S
Sbjct: 293 KLAKRTQSFRREDNANRERSTSRKPSADKAEKTDKTDAIAEKVSDIRLGDGEKKSSETDS 352

Query: 219 DL 224
           ++
Sbjct: 353 EV 354


>SPBC21C3.17c |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 186

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 11/40 (27%), Positives = 21/40 (52%)
 Frame = +2

Query: 404 WKLIPNYSLSNSFTLSDHMVKFNLSLRLKLCIILWYFFYY 523
           WK++ + S + S    D  +   LS+ L  CI++  F ++
Sbjct: 124 WKMVVDVSPAFSVNPIDKKLAIGLSVGLSCCILIVLFLHF 163


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,639,971
Number of Sequences: 5004
Number of extensions: 55912
Number of successful extensions: 127
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 127
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)